Molecular cloning and overexpression in HepG2 cells". Biochem. J. 277 ( Pt 3) (Pt 3): 659-64. doi:10.1042/bj2770659. PMC ... Schooltink H, Stoyan T, Lenz D, Schmitz H, Hirano T, Kishimoto T, Heinrich PC, Rose-John S (1991). "Structural and functional ... "HIV-gp 160-induced T cell-dependent B cell differentiation. Role of T cell-B cell activation molecule and IL-6". J. Immunol. ... "Human osteoclast-like cells are formed from peripheral blood mononuclear cells in a coculture with SaOS-2 cells transfected ...

"Human skin mast cell carboxypeptidase: functional characterization, cDNA cloning, and genealogy". The Journal of Investigative ... "Cloning of cDNAs that encode human mast cell carboxypeptidase A, and comparison of the protein with mouse mast cell ... A new mast cell subtype reported to appear in mucosa is implicated in allergic inflammation and these mast cells have high ... Chen ZQ, He SH (February 2004). "Cloning and expression of human colon mast cell carboxypeptidase". World Journal of ...

Consequently, all clone of cells derived from GSC are marked with a functional lacZ gene. By tracking the marked cells, they ... Stem cells will turn into certain cells as the body needs them. Stem cells divide more than non stem cells so the tendency of ... First, different cells may have different lifespans even though they originate from the same stem cells (See T-cells and ... have reviewed evidence that age-dependent accumulation of DNA damage in both stem cells and cells that comprise the stem cell ...

"Cloning and functional expression of endothelin-converting enzyme from rat endothelial cells". The Journal of Biological ... Cell. 78 (3): 473-85. doi:10.1016/0092-8674(94)90425-1. PMID 8062389. S2CID 7872049. Endothelin-converting+enzyme+1 at the U.S ...

Kang JS, Kohlhuber F, Hug H, Marmé D, Eick D, Ueffing M (1996). "Cloning and functional analysis of the hematopoietic cell- ... activation in erythropoietin-stimulated cells". Cell. Signal. 14 (10): 869-78. doi:10.1016/s0898-6568(02)00036-0. PMID 12135708 ... Cell. Biol. 13 (9): 5877-87. doi:10.1128/MCB.13.9.5877. PMC 360336. PMID 8395016. Sidorenko SP, Law CL, Klaus SJ, Chandran KA, ... Cell. Biol. 18 (12): 6951-61. doi:10.1128/mcb.18.12.6951. PMC 109278. PMID 9819383. Tomassetti A, Bottero F, Mazzi M, Miotti S ...

"Molecular cloning and functional expression of human connexin37, an endothelial cell gap junction protein". The Journal of ... Molecular cloning, expression, and chromosome mapping". The Journal of Biological Chemistry. 267 (3): 2057-64. doi:10.1016/ ... Seul KH, Kang KY, Lee KS, Kim SH, Beyer EC (July 2004). "Adenoviral delivery of human connexin37 induces endothelial cell death ... This protein, like other Connexin proteins, forms connections between cells known as gap junctions. Connexin 37 can be found in ...

"Cloning of the human platelet endothelial cell adhesion molecule-1 promoter and its tissue-specific expression. Structural and ... DeLisser HM, Newman PJ, Albelda SM (October 1994). "Molecular and functional aspects of PECAM-1/CD31". Immunology Today. 15 (10 ... macrophages and Kupffer cells, granulocytes, lymphocytes (T cells, B cells, and NK cells), megakaryocytes, and osteoclasts. In ... CD31 on endothelial cells binds to the CD38 receptor on natural killer cells for those cells to attach to the endothelium. ...

Staunton DE, Dustin ML, Springer TA (May 1989). "Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ... ICAM-2 molecules regulate spermatid adhesion on Sertoli cell on the apical side of the blood-testis barrier (towards the lumen ... This protein may also play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive ... Lalor PF, Shields P, Grant A, Adams DH (February 2002). "Recruitment of lymphocytes to the human liver". Immunology and Cell ...

This usually involves the cloning and in vitro modification of genes and proteins outside cells. Since the pioneering work of ... That is, within a single family of proteins numerous structural and functional mechanisms can evolve. Functional constructivism ... of a cell or virus. Mutations result from errors in DNA replication during cell division and by exposure to radiation, ... Functional evolution. Numerous enzymes and other proteins have been shown to change their function over the course of evolution ...

cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis". ... cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis". ... The Journal of Cell Biology. 140 (1): 49-60. doi:10.1083/jcb.140.1.49. PMC 2132588. PMID 9425153. Will GK, Soukupova M, Hong X ... The Journal of Cell Biology. 148 (5): 931-44. doi:10.1083/jcb.148.5.931. PMC 2174547. PMID 10704444. Gavva NR, Wen SC, Daftari ...

cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis". ... cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis". ... "Recognition of a functional peroxisome type 1 target by the dynamic import receptor pex5p". Molecular Cell. 24 (5): 653-63. doi ... The Journal of Cell Biology. 147 (4): 761-74. doi:10.1083/jcb.147.4.761. PMC 2156163. PMID 10562279. Okumoto K, Abe I, Fujiki Y ...

"Cloning and functional characterization of human septin 10, a novel member of septin family cloned from dendritic cells". ... "Are surrogates of IGHV gene mutational status useful in B-cell chronic lymphocytic leukemia? The example of Septin-10". ... "Identification of a novel role of Septin 10 in paclitaxel-resistance in cancers through a functional genomics screen". Cancer ...

"Genetic transfer of a functional human interferon alpha receptor into mouse cells: cloning and expression of its cDNA". Cell. ... 2015). "DNA-Damage-Induced Type I Interferon Promotes Senescence and Inhibits Stem Cell Function". Cell Reports. 11 (5): 785- ... Cell. Biol. 17 (4): 2048-56. doi:10.1128/mcb.17.4.2048. PMC 232052. PMID 9121453. Uddin S, Chamdin A, Platanias LC (Oct 1995 ... Cell. Biol. 14 (12): 8133-42. doi:10.1128/mcb.14.12.8133. PMC 359352. PMID 7526154. Uddin S, Chamdin A, Platanias LC (1995). " ...

Yamaguchi S (Jun 1995). "[Subtraction cloning of growth arrest inducible genes in normal human epithelial cells]". Kokubyo ... Gaither LA, Eide DJ (Mar 2000). "Functional expression of the human hZIP2 zinc transporter". J Biol Chem. 275 (8): 5560-4. doi: ...

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... cloning and functional expression of a truncated form in COS cells". Biochemical and Biophysical Research Communications. 169 ( ... Tateishi K, Arakawa F, Misumi Y, Treston AM, Vos M, Matsuoka Y (November 1994). "Isolation and functional expression of human ... Harrison JF, Greenlee KJ, Verberk WC (January 2018). "Functional Hypoxia in Insects: Definition, Assessment, and Consequences ... December 1989). "Molecular and functional characterization of amylin, a peptide associated with type 2 diabetes mellitus". ...

... molecular cloning and functional characterization as an hepatocyte-stimulating factor". Proc. Natl. Acad. Sci. U.S.A. 97 (18): ... 2004). "The T-cell lymphokine interleukin-26 targets epithelial cells through the interleukin-20 receptor 1 and interleukin-10 ... 1997). "Identification and functional characterization of a second chain of the interleukin-10 receptor complex". EMBO J. 16 ( ... Gibbs VC, Pennica D (1997). "CRF2-4: isolation of cDNA clones encoding the human and mouse proteins". Gene. 186 (1): 97-101. ...

... by a functional cloning to inhibit tumor necrosis factor-induced cell death". The Journal of Biological Chemistry. 277 (32): ... Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (June 2002). "Construction of expression-ready cDNA clones for ... The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 7 (1): 65-73. ... 2005). "A genome annotation-driven approach to cloning the human ORFeome". Genome Biology. 5 (10): R84. doi:10.1186/gb-2004-5- ...

... molecular cloning and functional characterization as an hepatocyte-stimulating factor". Proceedings of the National Academy of ... IL-22 takes effect on non-hematopoietic cells - mainly stromal and epithelial cells. Effects involve stimulation of cell ... IL-22 is produced by several populations of immune cells at a site of inflammation. Producers are αβ T cells classes Th1, Th22 ... December 2009). "Th22 cells represent a distinct human T cell subset involved in epidermal immunity and remodeling". The ...

2001). "Ca2+-activated K+ channels in human leukemic Jurkat T cells. Molecular cloning, biochemical and functional ... 2007). "Cell-cycle-dependent regulation of Ca2+-activated K+ channel in Jurkat T-lymphocyte". J. Pharmacol. Sci. 104 (1): 94-8 ... 2004). "Molecular identification and functional roles of a Ca2+-activated K+ channel in human and mouse hearts". J. Biol. Chem ... 2006). "Ca2+-activated K+ channels in human melanoma cells are up-regulated by hypoxia involving hypoxia-inducible factor-1α ...

"Functional cloning of ARM-1, an adhesion-regulating molecule upregulated in metastatic tumor cells". Clinical & Experimental ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. Qiu XB, Ouyang SY, Li CJ, Miao S, Wang L, ... Cell. 125 (4): 801-14. doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685. Jørgensen JP, Lauridsen AM, Kristensen P ... To meet such functional complexity, 19S regulatory particle contains at least 18 constitutive subunits. These subunits can be ...

1995). "Human fetal adrenal hydroxysteroid sulphotransferase: cDNA cloning, stable expression in V79 cells and functional ... 1995). "Human dehydroepiandrosterone sulfotransferase gene: molecular cloning and structural characterization". DNA Cell Biol. ... Cell. Endocrinol. 112 (1): 53-60. doi:10.1016/0303-7207(95)03585-U. PMID 7589785. S2CID 39327051. Luu-The V, Dufort I, Paquet N ... Cell Genet. 70 (1-2): 45-7. doi:10.1159/000133988. PMID 7736787. Falany CN, Wheeler J, Oh TS, Falany JL (1994). "Steroid ...

1993). "Cloning, functional expression and tissue distribution of human cDNA for the alpha 1C-adrenergic receptor". Biochem. ... 2003). "alpha 1-Adrenergic receptor subtypes differentially control the cell cycle of transfected CHO cells through a cAMP- ... 1995). "Cloning, functional expression and tissue distribution of human alpha 1c-adrenoceptor splice variants". FEBS Lett. 363 ... 1999). "Human cloned alpha1A-adrenoceptor isoforms display alpha1L-adrenoceptor pharmacology in functional studies". Eur. J. ...

mTECs are important for proper T-cell maturation and negative selection of autoreactive clones, required for development of ... Mechanistically, EFS expressed in medullary thymic epithelial cells (mTECs) is crucial for their functional maturation and ... Consistent with this mechanism, EFS overexpression in T cell-derived cell lines decreased IL-2 concentration in supernatants in ... EFS regulates T-cell function and maturation, preventing expansion of autoreactive clones and pathological immune responses. ...

"Benzophenone synthase and chalcone synthase from Hypericum androsaemum cell cultures: cDNA cloning, functional expression, and ... A passage cell is short cells that form a thin layer along the long axis of the plant exodermis. These cells are a structural ... Exodermal cells can be found very close to the tip of the root, with some plants demonstrating exodermal cells as close to 30 ... Similar to gap junctions found in animal cells, they allow an easy connection between the two cells permitting the transfer of ...

If the insert provided no resistance, the cell died and did not form a colony. Based on selection of cell colonies that ... This is not a requirement with functional cloning. TOPO Cloning is a cloning method that uses Taq polymerase. This is because ... A way to differentiate between functional and positional cloning is to visualize genes as words. Functional cloning is like ... As with other cloning techniques, vector and host choice affect the success of gene identification via functional cloning due ...

... a putative ATPase cloned by functional complementation on a peroxisome-deficient mammalian cell mutant". Nat. Genet. 11 (4): ...

For example, only isoform 1 expression is related to the cloning efficiency of embryonic stem cells. In a similar way to MSI1, ... they are different on a functional way and they play different roles in some aspects of the process. ... including stem cells, and both normal and leukemic blood cells. Musashi-2 also appears to be expressed in stem cells and in a ... MSI2 is linked to tissue stem cells and has an influence in asymmetric cell division, germ and somatic stem cell function and ...

The B cells in the body have two important phenotypes (functional forms)-the antibody secreting, terminally differentiated ( ... so they are technically a single clone of cells. However, during course of cell division, one of the cells can get mutated ... bone marrow cells. Most other cells cannot divide indefinitely as after a few cycles of cell division the cells stop expressing ... A clone is a group of identical cells that share a common ancestry, meaning they are derived from the same cell. Clonality ...