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*  Direct fluorescent antibody
Adam Reich, Katarzyna Marcinow, and Rafal Bialynicki-Birula Direct Fluorescent Antibody Technique at the US National Library of ... A direct fluorescent antibody (DFA or dFA), also known as "direct immunofluorescence", is an antibody that has been tagged in a ... Direct fluorescent antibody can also be used to detect parasitic infections, as was pioneered by Sadun, et al. (1960). ... where the primary antibody binds the target antigen, with a secondary antibody directed against the primary, and a tag attached ...
*  List of MeSH codes (E05)
... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 --- fluorescent antibody technique, direct MeSH E05.200.750.551 ... fluorescent antibody technique, direct MeSH E05.478.588.375.310 --- fluorescent antibody technique, indirect MeSH E05.478. ... fluorescent antibody technique MeSH E05.478.588.375.050 --- antibody-coated bacteria test, urinary MeSH E05.478.588.375.300 ... fluorescent antibody technique, indirect MeSH E05.200.750.551.790 --- periodic acid-schiff reaction MeSH E05.200.750.551.810 ...
*  List of MeSH codes (E01)
... fluorescent antibody technique, direct MeSH E01.450.495.225.230 --- fluorescent antibody technique, indirect MeSH E01.450. ... fluorescent antibody technique MeSH E01.450.495.225.050 --- antibody-coated bacteria test, urinary MeSH E01.450.495.225.225 ... fluorescent treponemal antibody absorption test MeSH E01.450.495.735.850.800 --- treponema immobilization test MeSH E01.450. ... enzyme multiplied immunoassay technique MeSH E01.450.495.410.380 --- immunosorbent techniques MeSH E01.450.495.410.380.200 --- ...
*  Cryptosporidiosis
This can be done with direct fluorescent antibody (DFA) techniques. It can also be achieved through indirect immunofluorescence ... Another form of microscopy is fluorescent microscopy done by staining with auramine. Other staining techniques include acid- ... vaccine has produced an antibody response in a large group of cows and also antibody response in calves fed rCP15/60-immune ... Antibodies in the serum of humans and animals infected with Cryptosporidium parvum react with several antigens, one of which is ...
*  Immunofluorescence
... an antigen can also be conjugated to the antibody with a fluorescent probe in a technique called fluorescent antigen technique ... Since the number of fluorescent molecules that can be bound to the primary antibody is limited, direct immunofluorescence is ... This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets ... There are two classes of immunofluorescence techniques, primary (or direct) and secondary (or indirect). Primary (direct) ...
*  FluoProbes
They are designed for labeling biomolecules, cells, tissues or beads in advanced fluorescent detection techniques. FluoProbes ... while FluoProbes labeled antibodies and cellular probes (i.e. Phalloidin) suit direct use in immunoassays or cell assays. ... Similar lines of fluorescent dyes provide an alternative to the FluoProbes Dyes. "FluoProbes Dyes" (PDF). Interchim. 2010. ... The FluoProbes series of fluorescent dyes were developed by Interchim to improve performances of standard fluorophores. ...
*  Syphilis
... direct fluorescent antibody testing and nucleic acid amplification tests. Direct fluorescent testing uses antibodies tagged ... with fluorescein, which attach to specific syphilis proteins, while nucleic acid amplification uses techniques, such as the ... or fluorescent treponemal antibody absorption test (FTA-Abs). Treponemal antibody tests usually become positive two to five ... Confirmation is either via blood tests or direct visual inspection using microscopy. Blood tests are more commonly used, as ...
*  Rabies
6: The fluorescent antibody test". In Kaplan, M.M.; Koprowski, H. Laboratory techniques in rabies. Monograph series. 23 (3rd ed ... Microscopic analysis of samples is the only direct method that allows for the identification of rabies virus-specific antigen ... The reference method for diagnosing rabies is the fluorescent antibody test (FAT), an immunohistochemistry procedure, which is ... to bind to and allow the visualisation of rabies antigen using fluorescent microscopy techniques. ...
*  Infection
For example, the use of antibodies made artificially fluorescent (fluorescently labeled antibodies) can be directed to bind to ... Complex serological techniques have been developed into what are known as Immunoassays. Immunoassays can use the basic antibody ... Immunity mediated by these two factors may be manifested by: a direct effect upon a pathogen, such as antibody-initiated ... Transmission by direct contact, Some diseases that are transmissible by direct contact include athlete's foot, impetigo and ...
*  Neuronal lineage marker
Immunohistochemistry is a technique that uses antibodies with fluorescent staining tags that target a specific antigen present ... Cellular fates are analysed by staining with antibodies directed against antigens specific for astrocytes, oligodendrocytes, ... He created an immunoflorescent technique for labelling the antibodies. This technique continues to be widely used in ... Albert Coons used for the first time a revolutionary technique that uses the principle of antibodies binding specifically to ...
*  DFA
... the process of establishing a generation-by-generation descent of living persons Direct fluorescent antibody, a medical test ... a technique for gathering information about the possible set of values calculated at various points in a computer program ... a variation of the Hurst Exponent technique, used in the analysis of fractal time series Discriminant function analysis, a ...
*  Protein
This technique also uses an antibody to the protein of interest, along with classical electron microscopy techniques. The ... Fluorescent dyes are used to label cellular compartments for a similar purpose. Other possibilities exist, as well. For example ... Methods commonly used to study protein structure and function include immunohistochemistry, site-directed mutagenesis, X-ray ... antibodies have no such constraints. An antibody's binding affinity to its target is extraordinarily high. Many ligand ...
*  Willy Burgdorfer
Burgdorfer, W. Evaluation of the fluorescent antibody technique for the detection of Rocky Mountain spotted fever rickettssiae ... he directed the WHO-sponsored Reference Center for Rickettsial Diseases at RML in Montana, U.S. Burgdorfer was critical of the ... A technique for detection of rickettsiae in ticks, Amer. J. Trop. Med. 19:1010-1014, 1970. Burgdorfer, W., Barbour, A. G., ...
*  Immunology
Antibodies specific for a desired antigen can be conjugated with an isotopic (radio) or fluorescent label or with a color- ... and antigen has made the antibody an excellent tool for the detection of substances by a variety of diagnostic techniques. ... Symbiont-mediated defenses are also heritable across host generations, despite a non-genetic direct basis for the transmission ... The humoral (antibody) response is defined as the interaction between antibodies and antigens. Antibodies are specific proteins ...
*  Fluorescence polarization immunoassay
Despite originating as a method for direct interaction studies, the technique has been adopted by high-throughput screening ( ... The fluorescent lifetime is the amount of time between the absorption moment and the fluorescent emission moment. Typically, ... Detection of Antibody to Brucella abortus". Methods. 22: 71-76 - via Elsevier Science Direct. Jameson, David (2003). " ... However, if the excited fluorescent molecule is in motion (rotational or translational) during the fluorescent lifetime, it ...
*  Single-domain antibody
Screening techniques like phage display and ribosome display help to identify the clones binding the antigen. A different ... The fusion of a fluorescent protein to a nanobody generates a so-called chromobody. Chromobodies can be used to recognize and ... In mice infected with influenza A virus subtype H5N1, Nanobodies directed against hemaglutinin suppressed replication of the ... is an antibody fragment consisting of a single monomeric variable antibody domain. Like a whole antibody, it is able to bind ...
*  Immunostaining
... is perhaps the most commonly applied immunostaining technique. While the first cases of IHC staining used fluorescent dyes (see ... The primary antibody can be directly labeled using an enzyme or fluorophore. The primary antibody can be labeled using a small ... A flow cytometer can be used for the direct analysis of cells expressing one or more specific proteins. Cells are immunostained ... The primary antibody can be probed for using a broader species-specific secondary antibody that is labeled using an enzyme, or ...
*  Infectious hematopoietic necrosis virus
... indirect fluorescent antibody testing, ELISA, PCR, and DNA probe technology techniques can be used to confirm diagnosis. The ... IHNV is transmitted following shedding of the virus in the feces, urine, sexual fluids, and external mucus and by direct ...
*  Thymidine kinase
Those that produce the desired antibody are then selected and cultured to produce the monoclonal antibody. Hybridoma cells can ... The technique has also been validated for lung cancer, for colorectal carcinima, for lung cancer and for renal cell carcinoma. ... Direct determination of the thymidine kinase protein by immunoassay has also been used. The amounts of thymidine kinase found ... This reagent together with a fluorescent azide cause fluorescence of infected cells but not of uninfected cells. Therefore, ...
*  Circulating tumor cell
Antibodies against tumor specific biomarkers including EpCAM, Her2, PSA are used. The most common technique is magnetic ... Like CellSearch maintrac uses an EpCAM antibody. It is, however, not used for enrichment but rather as a fluorescent marker to ... The captured cells are ready for cell culture or for direct characterization using ViewRNA in situ hybridization assay. Another ... All nucleated cells, including normal white blood cells and CTCs, are exposed to fluorescent-tagged antibodies specific for ...
*  Photobleaching
However, photobleaching may also be used prior to applying the (primarily antibody-linked) fluorescent molecules, in an attempt ... or FLIP techniques, in which multiple rounds of photobleaching is done so that the spread of fluorescence loss can be observed ... "An improved and cost-effective methodology for the reduction of autofluorescence in direct immunofluorescence studies on ... In microscopy, photobleaching may complicate the observation of fluorescent molecules, since they will eventually be destroyed ...
*  Glycome
Anyway, direct MALDI-TOF-MS analysis can lead to a fast and straightforward illustration of the glycan pool. In recent years, ... This technique is being used to characterize the immune glycome. Table 1:Advantages and disadvantages of mass spectrometry in ... This method uses either naturally occurring lectins or artificial monoclonal antibodies, where both are immobilized on a ... January 2009). "Comparison of fluorescent labels for oligosaccharides and introduction of a new postlabeling purification ...
*  Thermal shift assay
Additionally, instead of using two antibodies the FRET method could be performed with a single fluorescent antibody and a ... A technique that has been shown to work on both complex mixtures and whole cells but unfortunately requires antibodies. The ... For pure or highly enriched proteins, direct SDS-PAGE detection is possible facilitating Commassie-fluorescence based direct ... The technique was developed by a team led by Patrick Schaeffer at James Cook University and published in Moreau et al. 2012. ...
*  Immunoperoxidase
Alternatively, the primary antibody can be detected using fluorescent label (immunofluorescence), or be attached to colloidal ... Related techniques are also useful in sub-typing lymphocytes which all look quite similar on light microscopy. In laboratory ... Other catalytic enzymes such as alkaline phosphatase can be used instead of peroxidases for both direct and indirect staining ... Example 3. An untagged primary antibody is detected using a general secondary antibody that recognises all antibodies ...
*  Immunoprecipitation
Antibody can be attached to solid support before this step (direct method) or after this step (indirect method). Continue the ... Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that ... followed by detection using a chemiluminescent or fluorescent secondary antibody. Keene JD, Komisarow JM, Friedersdorf MB (2006 ... irrelevant antibody of the same antibody subclass as the IP antibody is used instead of the IP antibody itself. This approach ...
*  Bovine spongiform encephalopathy
After amplifying and then concentrating any PrPSc, the samples are labelled with a fluorescent dye using an antibody for ... The technique allowed detection of PrPSc after many fewer cycles of conversion than others have achieved, substantially ... For many of the vCJD patients, direct evidence exists that they had consumed tainted beef, and this is assumed to be the ... Tiwana H, Wilson C, Pirt J, Cartmell W, Ebringer A (1999). "Autoantibodies to brain components and antibodies to Acinetobacter ...