Taraxein
I: Demonstration of antibrain globulins by fluorescent antibody techniques. Arch Gen Psychiat 16: 1-9. (Schizophrenia, Blood ...
Cryptosporidiosis
This can be done with direct fluorescent antibody (DFA) techniques. It can also be achieved through indirect immunofluorescence ... Another form of microscopy is fluorescent microscopy done by staining with auramine. Other staining techniques include acid- ... vaccine has produced an antibody response in a large group of cows and also antibody response in calves fed rCP15/60-immune ... Antibodies in the serum of humans and animals infected with Cryptosporidium parvum react with several antigens, one of which is ...
International Society for Microbial Ecology
Also, differential staining methods and fluorescent antibody techniques were introduced for in-situ observation of ... Various new techniques, ranging from the determination of growth and activity of microorganisms in the environments to their ... The new methods and techniques were proved to be successful for the analysis of microbial community in various fields, soil and ...
Pseudomonas melophthora
... by fluorescent-antibody technique". Journal of Invertebrate Pathology. 11 (2): 251-259. doi:10.1016/0022-2011(68)90158-4. ...
Mary Osborn
Later, Osborn and Weber pioneered fluorescent antibody staining of cellular substructures, a major technique called indirect ... then used fluorescently-tagged secondary antibodies (antibodies to the first set of antibodies) to light up the locations of ... and unreactive to actin antibodies. They developed new antibodies against proteins of the microtubules, intermediate filaments ... Many of their antibodies have been licensed to companies for commercial development. Klaus and Osborn used their method to ...
Willy Burgdorfer
Burgdorfer, W. Evaluation of the fluorescent antibody technique for the detection of Rocky Mountain spotted fever rickettsiae ... A technique for detection of rickettsiae in ticks, Amer. J. Trop. Med. 19:1010-1014, 1970. Burgdorfer, W., Barbour, A. G., ...
Spring viraemia of carp
... fluorescent antibody and cell culture isolation techniques for detection of antigen". Journal of Fish Diseases. 7: 57-64. doi: ...
Direct fluorescent antibody
Adam Reich, Katarzyna Marcinow, and Rafal Bialynicki-Birula Direct+Fluorescent+Antibody+Technique at the U.S. National Library ... A direct fluorescent antibody (DFA or dFA), also known as "direct immunofluorescence", is an antibody that has been tagged in a ... Direct fluorescent antibody can also be used to detect parasitic infections, as was pioneered by Sadun, et al. (1960). ... where the primary antibody binds the target antigen, with a secondary antibody directed against the primary, and a tag attached ...
Elvio Sadun
... he and two other researchers were the first to apply a recently developed technique, fluorescent antibody imaging, to the ... "Home". Sadun, E.H, Williams, J.S, Anderson, R.I. Fluorescent antibody technic for sero-diagnosis of schistosomiasis in humans. ... and is known for the first application of fluorescent antibody imaging in the diagnosis of parasitic diseases. Sadun was born ...
Neuronal lineage marker
Immunohistochemistry is a technique that uses antibodies with fluorescent staining tags that target a specific antigen present ... He created an immunoflorescent technique for labelling the antibodies. This technique continues to be widely used in ... Albert Coons used for the first time a revolutionary technique that uses the principle of antibodies binding specifically to ... These examples use FISH (Fluorescent in situ hybridization). With this technique we can understand the physiological processes ...
Spatiotemporal gene expression
This distribution of this antibody can then be visualized by a technique such as fluorescent labeling. Immunohistochemistry has ... In this technique, DNA that encodes a reporter gene is randomly inserted into the genome. Depending on the gene promoters ... Techniques that require fixation of tissue can only generate a single temporal time point per individual organism. However, ... For example, the reporter gene green fluorescent protein can be visualized by stimulating it with blue light and then using a ...
Gaffkaemia
To reduce the four-day waiting time needed for diagnosis, a method using the indirect fluorescent antibody technique (IFAT) was ...
Richard O. Spertzel
Benador Associates ^ "Detection of air-borne Pasteurella tularensis using the fluorescent antibody technique" by R. F. Jaeger, ...
Ifat
... a Muslim sultanate in the northern Horn of Africa Indirect fluorescent antibody technique, a diagnostic process employing ...
List of MeSH codes (E05)
... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 - fluorescent antibody technique, direct MeSH E05.200.750.551. ... fluorescent antibody technique, direct MeSH E05.478.588.375.310 - fluorescent antibody technique, indirect MeSH E05.478.588.375 ... fluorescent antibody technique MeSH E05.478.588.375.050 - antibody-coated bacteria test, urinary MeSH E05.478.588.375.300 - ... 512.240.310 - fluorescent antibody technique, indirect MeSH E05.200.750.551.790 - periodic acid-schiff reaction MeSH E05.200. ...
Cornelia Mitchell Downs
She is also well known for her work in the development of the fluorescent antibody technique-a diagnostic technique used to ... "Isothiocyanate Compounds as Fluorescent Labeling Agents for Immune Serum". The American Journal of Pathology. 34 (6): 1081-1097 ...
List of MeSH codes (E01)
... fluorescent antibody technique, direct MeSH E01.450.495.225.230 - fluorescent antibody technique, indirect MeSH E01.450.495.225 ... fluorescent antibody technique MeSH E01.450.495.225.050 - antibody-coated bacteria test, urinary MeSH E01.450.495.225.225 - ... fluorescent treponemal antibody absorption test MeSH E01.450.495.735.850.800 - treponema immobilization test MeSH E01.450. ... enzyme multiplied immunoassay technique MeSH E01.450.495.410.380 - immunosorbent techniques MeSH E01.450.495.410.380.200 - ...
Infectious hematopoietic necrosis virus
... indirect fluorescent antibody testing, ELISA, PCR, and DNA probe technology techniques can be used to confirm diagnosis. The ...
Edwardsiella tarda
... be cultured on specific growth mediums such as brain-heart infusion agar and techniques such as indirect fluorescent antibody ...
Immunofluorescence
... an antigen can also be conjugated to the antibody with a fluorescent probe in a technique called fluorescent antigen technique ... This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets ... recognizes the primary antibody and binds to it. Multiple secondary antibodies can bind a single primary antibody. This ... Since the number of fluorescent molecules that can be bound to the primary antibody is limited, direct immunofluorescence is ...
Escherichia coli O157:H7
... coli DNA extraction method plus polymerase chain reaction techniques. Newer technologies using fluorescent and antibody ... Tzipori S, Sheoran A, Akiyoshi D, Donohue-Rolfe A, Trachtman H (October 2004). "Antibody therapy in the management of shiga ... such as the use of anti-induction strategies to prevent toxin production and the use of anti-Shiga toxin antibodies, have also ...
Rabies
6: The fluorescent antibody test". In Kaplan MM, Koprowski H (eds.). Laboratory techniques in rabies. Monograph series. Vol. 23 ... The reference method for diagnosing rabies is the fluorescent antibody test (FAT), an immunohistochemistry procedure, which is ... to bind to and allow the visualisation of rabies antigen using fluorescent microscopy techniques. Microscopic analysis of ... Bat rabies antibodies (but not the virus) have been found in bats. On Svalbard, animals can cross the arctic ice from Greenland ...
STED microscopy
These cells are STED fluorescent dyes bound to antibodies through amide bonds. The first use of this technique coupled MR-121SE ... a red dye, with a secondary anti-mouse antibody. Since that first application, this technique has been applied to a much wider ... However, only fluorescent proteins provide the ability to visualize any organelle or protein in a living cell. This method was ... Using two fluorescent dyes and beam pairs, colocalized imaging of synaptic and mitochondrial protein clusters is possible with ...
Echinococcus
Antiechinococcus antibodies can be detected with serodiagnostic tests - indirect fluorescent antibody, complement fixation, ... Cysts are detected with ultrasound, X-ray computed tomography, or other imaging techniques. ...
Expansion microscopy
After expansion, the tissue is far less dense and often allows for better reception of fluorescent antibodies. Markoff J (2015- ... However, this technique has since been adopted into many different fields of research and continues to grow and be applied in ... "Expansion microscopy with conventional antibodies and fluorescent proteins". Nature Methods. 13 (6): 485-8. doi:10.1038/nmeth. ... Expansion microscopy with conventional antibodies and fluorescent proteins. Nature Methods, 2016, 13, 485-488. "Microscopy ...
Complete blood count
... such as fluorescent staining, multi-angle light scatter and monoclonal antibody tagging. Most analyzers directly measure the ... In light scattering techniques, light from a laser or a tungsten-halogen lamp is directed at the stream of cells to collect ... These techniques work on the same principle of measuring the interruption in current as cells pass through an aperture, but ... The first techniques for measuring hemoglobin were devised in the late 19th century, and involved visual comparisons of the ...
High-content screening
... or by fluorescent antibodies. The technology may be used to determine whether a potential drug is disease modifying. For ... in the amounts of proteins synthesized by cells are measured using a variety of techniques such as the green fluorescent ... The wide use of the green fluorescent protein, a natural fluorescent protein molecule from jellyfish, then accelerated the ... In addition to fluorescent labeling, various label free assays have been used in high content screening. High-content screening ...
Single-cell analysis
... fluorescent protein based methods, and mass-spectroscopy based methods. The antibody based methods use designed antibodies to ... Secondary ion mass spectrometry (SIMS) is a technique similar to DESI, but while DESI is an ambient ionization technique, SIMS ... Imaging: Antibodies can be bound to fluorescent molecules such as quantum dots or tagged with organic fluorophores for ... Antibody-DNA quantification: another antibody-based method converts protein levels to DNA levels. The conversion to DNA makes ...
Nanoscale secondary ion mass spectrometry
In a separate study, antibody-antigen binding was studied without the need for a fluorescent label to be added to the antibody ... Other microscopy techniques are commonly used in tandem with NanoSIMS that allow for multiple types of information to be ... Immunogold labeling uses antibodies to target specific proteins, and subsequently labels the antibodies with gold nano ... This technique can be used to study nutrient exchange. The mouse gut microbiome was investigated to determine which microbes ...
Infection
For example, the use of antibodies made artificially fluorescent (fluorescently labeled antibodies) can be directed to bind to ... Complex serological techniques have been developed into what are known as immunoassays. Immunoassays can use the basic antibody ... and can be made exquisitely specific when used in combination with antibody based techniques. ... Other techniques (such as X-rays, CAT scans, PET scans or NMR) are used to produce images of internal abnormalities resulting ...
Histology
This process is called immunohistochemistry, or when the stain is a fluorescent molecule, immunofluorescence. This technique ... Recently, antibodies have been used to specifically visualize proteins, carbohydrates, and lipids. ... Ramón y Cajal won the prize for his correct theory, and Golgi for the silver-staining technique that he invented to make it ... In contrast to H&E, which is used as a general stain, there are many techniques that more selectively stain cells, cellular ...