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*  Molecular cloning
... a selectable genetic marker gene that can be used to enable the survival of cells that have taken up vector sequences a tag ... These include--(1) medically useful proteins whose administration can correct a defective or poorly expressed gene (e.g. ... The idea arose that different DNA sequences could be inserted into a plasmid and that these foreign sequences would be carried ... vector DNA with no recombinant sequence inserted). In these vectors, foreign DNA is inserted into a sequence that encodes an ...
*  Expressed sequence tag
In genetics, an expressed sequence tag (EST) is a short sub-sequence of a cDNA sequence. ESTs may be used to identify gene ... Because of the way ESTs are sequenced, many distinct expressed sequence tags are often partial sequences that correspond to the ... Jun 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. doi: ... In an effort to reduce the number of expressed sequence tags for downstream gene discovery analyses, several groups assembled ...
*  Warren Gish
Boguski, M.S.; Lowe, T.M.; Tolstoshev, C.M. (1993). "dbEST--database for "expressed sequence tags"". Nature Genetics. 4 (4): ... in part to aid multiplexing with MPBLAST and in part to aid analysis of segmented query sequences from shotgun sequencing ... Construction of the DFA was O(n), where n is the sum of the lengths of the query sequences. The DFA could then be used to scan ... Sense from Sequences: Stephen F. Altschul on Bettering BLAST Aho, Alfred V.; Corasick, Margaret J. (June 1975). "Efficient ...
*  Transcriptomics technologies
... low-throughput sequencing using the Sanger method was used to sequence random transcripts, producing Expressed Sequence Tags ( ... An expressed sequence tag (EST) is a short nucleotide sequence generated from a single RNA transcript. RNA is first copied as ... Legend: RAM - random access memory; MPI - message passing interface; EST - expressed sequence tag. Quantification of sequence ... June 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. PMID ...
*  NAS Award for the Industrial Application of Science
... using expressed sequence tags in genetic analyses, ... sequencing of microbial genomes, and his leadership in ... sequencing ...
*  Digital transcriptome subtraction
using human expressed sequence tag (EST) datasets. In a proof of principle experiment, Meyerson et al. demonstrated that it was ... Other vertebrate sequences Unannotated sequences After stringent rounds of subtraction, the remaining sequences are clustered ... The cDNA library must be sequenced to great depth (i.e. number of clones sequenced) in order to detect a theoretical rare ... BLAST and exclude E. coli genome sequences. Using MEGABLAST, Hi-Fi reads are then matched to sequences in annotated databases ...
*  CD34
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... Human HSCs express the CD34 marker.[citation needed] CD34 is expressed in roughly 20% of murine hematopoietic stem cells, and ... It is expressed on lymph node endothelia, whereas the L-selectin to which it binds is on the T cell. Conversely, under other ... Cells expressing CD34 (CD34+ cell) are normally found in the umbilical cord and bone marrow as hematopoietic cells, or in ...
*  RBMX
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... This gene, an active X chromosome homolog of the Y chromosome RBMY gene, is widely expressed whereas the RBMY gene evolved a ... sequence and characterization of a glycosylated RNA-binding protein". Nucleic Acids Research. 21 (18): 4210-7. doi:10.1093/nar/ ...
*  BANP
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ...
*  GPR34
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins ...
*  Cyclic nucleotide-gated channel alpha 1
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... amino acid sequence, gene structure, and functional expression". The Journal of Neuroscience. 12 (8): 3248-56. PMID 1379636. ... exchanger oligomers with cGMP-gated channel subunits as probed with heterologously expressed cDNAs". Biochemistry. 42 (15): ... "Identification and characterization of a partial cDNA expressing interleukin-1-like activity in keratinocytes". Molecular ...
*  RBBP5
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... The protein encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD- ...
*  SORBS2
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... ArgBP2 is highly abundant in cardiac muscle cells at sarcomeric Z-disc structures, and is expressed in other cells at actin ... sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research. 11 (3): 422-35. doi:10.1101/gr. ...
*  SH3KBP1
September 1996). "Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi: ... "Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence" (PDF). ...
*  ETV6
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... which binds to the consensus DNA sequence, 5-GGAA/T-3 within a 9-to-10 bp sequence, in the target genes it regulates. ETV6 ... Fainstein E, Einat M, Gokkel E, Marcelle C, Croce CM, Gale RP, Canaani E (Dec 1989). "Nucleotide sequence analysis of human abl ... the gene is expressed in virtually all cell types and tissues. Mice depleted of the ETV6 gene by Gene knockout die between day ...
*  Haptoglobin
"Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... In addition, the haptoglobin gene is expressed in murine and human adipose tissue. Haptoglobin had been shown to be expressed ... Raugei G, Bensi G, Colantuoni V, Romano V, Santoro C, Costanzo F, Cortese R (1983). "Sequence of human haptoglobin cDNA: ... Maeda N (1985). "Nucleotide sequence of the haptoglobin and haptoglobin-related gene pair. The haptoglobin-related gene ...
*  UHRF1
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... "A new growth-regulated complementary DNA with the sequence of a putative trans-activating factor". Cell Growth & ...
*  Sphingosine kinase 1
Sudo K, Chinen K, Nakamura Y (Nov 1994). "2058 expressed sequence tags (ESTs) from a human fetal lung cDNA library". Genomics. ... "Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ...
*  HIVEP2
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... These proteins bind specific DNA sequences, including the kappa-B motif (GGGACTTTCC), in the promoters and enhancer regions of ... protein structure consisting of a pair of C2H2 zinc fingers with an acidic-rich region and a serine/threonine-rich sequence. ...
*  Oct-4
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... Wey E, Lyons GE, Schäfer BW (Mar 1994). "A human POU domain gene, mPOU, is expressed in developing brain and specific adult ... However, in cells that are not actively expressing Oct3/4, an exogenous transfection of Oct3/4 will lead to the activation of ... Ben-Shushan E, Thompson JR, Gudas LJ, Bergman Y (Apr 1998). "Rex-1, a gene encoding a transcription factor expressed in the ...
*  CHRNA7
"Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... The CHRNA7 gene is primarily expressed in the posterior amygdalar nucleus and the field CA3 of Ammon's horn in the mouse, and ... An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a ... Groot Kormelink PJ, Luyten WH (1997). "Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic ...
*  SMPD4
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 7 (1): 65-73. ... Nagase T, Kikuno R, Ishikawa KI, Hirosawa M, Ohara O (Feb 2000). "Prediction of the coding sequences of unidentified human ... sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research. 11 (3): 422-35. doi:10.1101/gr. ...
*  CNOT7
"Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... Adams MD, Dubnick M, Kerlavage AR, Moreno R, Kelley JM, Utterback TR, Nagle JW, Fields C, Venter JC (March 1992). "Sequence ...
*  CMAS (gene)
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... "NotI flanking sequences: a tool for gene discovery and verification of the human genome". Nucleic Acids Research. 30 (14): 3163 ... "Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence" (PDF). ...
*  ADARB2
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... Adenosine deamination of pre-mRNA results in a change in the amino acid sequence of the gene product, which differs from that ... predicted by the genomic DNA sequence. Other members of this family include DRADA (ADAR) and RED1 (ADARB1). Unlike ADAR1 and ...
*  Heroes and Villains
"Part 2" is the group's brief cover of "Gee" later sequenced to follow "Our Prayer". For Brian Wilson Presents Smile, "Gee" was ... Bandmate Al Jardine later expressed dissatisfaction with the final composite, calling it "a pale facsimile" of Wilson's ... ", "Part 1 Tag", and "All Day" were also recorded, and so was a keyboard/vocal section called "Bicycle Rider". "Cantina", ... with no specific designation on how it would fit in sequence within the song's final edit. However, the outro consisted of the ...
*  Lumican
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... Lumican is highly expressed in pleural effusions (lung fluid) of patients with adenocarcinoma. Its expression was low in cancer ... "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proceedings of the National ... "Lumican is down-regulated in cells expressing endoglin. Evidence for an inverse correlationship between Endoglin and Lumican ...