Yang, Z.; Sismour, A.M.; Sheng, P.; Puskar, N.L.; Benner, S.A. (2007). "Enzymatic incorporation of a third nucleobase pair". ... Noren, C.J.; Anthony-Cahill, S.J.; Griffith, M.C.; Schultz, P.G. (1989). "A general method for site-specific incorporation of ... Altering the codon: tRNA synthetases pairs may lead to the in vivo incorporation of the non-canonical amino acids into proteins ... Although proteins are the most common components of cellular enzymatic activity, nucleic acids are also used in the cell to ...
Hurwitz J (1959). "The enzymatic incorporation of ribonucleotides into polydeoxynucleotide material". J. Biol. Chem. 234: 2351- ...
Hurwitz J (1959). "The enzymatic incorporation of ribonucleotides into polydeoxynucleotide material". J. Biol. Chem. 234: 2351- ... gene expression and enzymatic activity coinciding with cell cycle progression and cell growth". DNA Cell Biol. 13 (5): 461-471 ...
Veedu, Rakesh N.; Vester, Birte; Wengel, Jesper (2007-03-26). "Enzymatic Incorporation of LNA Nucleotides into DNA Strands". ... This structure provides for increased stability against enzymatic degradation. LNA also offers improved specificity and ... Incorporation of LNA-Type Monomers into DNAzymes Markedly Increases RNA Cleavage". Journal of the American Chemical Society. ...
With respect to fuel cells, enzymes have several advantages to their incorporation. An important enzymatic property to consider ... An enzymatic biofuel cell is a specific type of fuel cell that uses enzymes as a catalyst to oxidize its fuel, rather than ... A fully enzymatic hydrogen fuel cell was constructed by the Armstrong group who used the cell to power a watch. The fuel cell ... Enzymatic biofuel cells work on the same general principles as all fuel cells: use a catalyst to separate electrons from a ...
Switzer C, Moroney SE, Benner SA (1989). "Enzymatic incorporation of a new base pair into DNA and RNA". J. Am. Chem. Soc. 111 ( ... The successful incorporation of a third base pair is a significant breakthrough toward the goal of greatly expanding the number ... September 2006). "An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA ... Trautner TA, Swartz MN, Kornberg A (March 1962). "Enzymatic synthesis of deoxyribonucleic acid. X. Influence of bromouracil ...
Switzer, Christopher; Moroney, Simon E.; Benner, Steven A. (October 1989). "Enzymatic incorporation of a new base pair into DNA ... "Enzymatic incorporation of a new base pair into DNA and RNA extends the genetic alphabet". Nature. 343 (6253): 33-37. Bibcode: ... Switzer, CY; Moroney, SE; Benner, SA (5 October 1993). "Enzymatic recognition of the base pair between isocytidine and ...
Biosynthetic incorporation of the aminobutyl group of spermidine into pyrrolizidine alkaloids. Evolution: the gene for ... Pyrrolizidine alkaloids Biosynthesis: importance of putrescine and spermidine as substrates of enzymatic homospermidine ... G. Graser, T. Hartmann: Biosynthetic incorporation of the aminobutyl group of spermidine into pyrrolizidine alkaloids, in ... Putrescine and spermidine are essential substrates of enzymatic homospermidine formation, in Canadian Journal of Chemistry ( ...
Osborn MJ, D'Ari L (August 1964). "Enzymatic incorporation of N-acetylglucosamine into cell wall lipopolysaccharide in a mutant ...
described an enzymatic pathway for the synthesis of psilocybin and psilocin, publishing their results in 2017. Sherwood et al. ... Incorporation of labelled tryptamine derivatives". Acta Chemica Scandinavica. 22 (4): 1210-1218. doi:10.3891/acta.chem.scand.22 ... Fricke J, Blei F, Hoffmeister D (September 2017). "Enzymatic synthesis of psilocybin". Angewandte Chemie. 56 (40): 12352-12355 ...
This inclusion is crucial for enzymatic activity. Vicilin is made up of one α subunit, a single glycerol, and a phosphate ion. ... This axis is responsible for all copper ligand incorporation. This copper center's structure has four main residues: Cys-338, ... The enzymatic activity is connected to copper binding via histidine residues. These copper ligands act catalytically on ...
PDB: 3VSY​; Kobe A, Caaveiro JM, Tashiro S, Kajihara D, Kikkawa M, Mitani T, Tsumoto K (March 2013). "Incorporation of rapid ... Pollack RM (October 2004). "Enzymatic mechanisms for catalysis of enolization: ketosteroid isomerase". Bioorganic Chemistry. 32 ... implicating Asp-99 as important for enzymatic activity. Wu et al. proposed a mechanism that involves both Tyr-14 and Asp-99 ... the Herschlag group utilized unnatural amino acid incorporation to assay the importance of Tyr-14 to KSI catalysis. The natural ...
The amino acid incorporation which forms the E-ring is a chemical reaction, rather than enzymatic, an uncommon occurrence in ... Studies have implicated JadG, an FAD-dependent oxygenase, in the ring cleavage required for incorporation of amino acids. JadS ... incorporation of non-natural and natural amino acids". Bioorganic & Medicinal Chemistry Letters. 15 (5): 1447-1449. doi:10.1016 ... Implications for Non-enzymatic Natural Products Biosynthesis". Journal of the American Chemical Society. 137 (9): 3271-3275. ...
... allowing for the incorporation and protection against enzymatic degradation. Gelatin Microparticles are versatile particles and ...
Post-synthesis labeling avoids the problems found in direct enzymatic incorporation of Cy-labeled dNTPs by generating probes ... After the enzymatic addition of the NTPs, amine reactant fluorescent dyes can be added for detection of the DNA molecule. When ... the method of aminoallyl nucleotide incorporation was opted instead. Using aminoallyl nucleotides as indirect fluorescent ...
For example, the incorporation of diarylethenes as a self-assembled monolayer on a gold surface shows promise in optoelectronic ... regulation of enzymatic activity, and photoregulated ion channels. For example, optical control of ligand binding in human ... Incorporation of photoswitching molecules such as donor-acceptor Stenhouse adducts into polymersomes has been used to form ... Incorporation of photoswitches into nematic liquid crystals can change self-assembly, crystal packing, and the light reflecting ...
Biosensors, tissue engineering, drug delivery, or enzymatic catalysis is just a few of the possible examples. The incorporation ...
Once the Argonaute is associated with the small RNA, the enzymatic activity conferred by the PIWI domain cleaves only the ... RNA strand separation and incorporation into the Argonaute protein are guided by the strength of the hydrogen bond interaction ... PfAgo based artificial restriction enzymes were also used for storing data on native DNA sequences via enzymatic nicking. Cox ... April 2020). "DNA punch cards for storing data on native DNA sequences via enzymatic nicking". Nature Communications. 11 (1): ...
The pyrophosphates are a necessary component of the nucleotide incorporation in the RNA replication. Pyrophosphate inhibitors ... During the nucleotidyl-transfer reaction, an enzymatic reaction fundamental for DNA synthesis, pyrophosphate is released, which ...
The incorporation of polymer chains by performing in situ ATRP on the outer surface of or inside the protein nanocages can be ... They benefit from monodispersity, intrinsic high stability for protection of internalized drugs from enzymatic degradation and ... Gauthier, Marc A.; Klok, Harm-Anton (2010). "Polymer-protein conjugates: an enzymatic activity perspective". Polymer Chemistry ... mechanism for increasing in vitro enzymatic resilience, and siRNA release characteristics". Acta Biomaterialia. 6 (11): 4277- ...
The methodology is widely used for enzymatic synthesis of DNA or RNA-bearing fluorescent, redox, or reactive labels, as well as ... followed by polymerase incorporation. They have discovered some modified dNTPs that are better substrates for polymerases than ... Hocek M (2014). "Synthesis of Base-Modified 2'-Deoxyribonucleoside Triphosphates and Their Use in Enzymatic Synthesis of ... Better Substrates for DNA Polymerases than dATP in Competitive Incorporations". Angew. Chem. Int. Ed. 53 (29): 7552-7555. doi: ...
Membrane fusion activity helps in incorporation viral genome into the host cell cytoplasm by enhancing the attachment between ... proteins that encompass enzymatic activities, whereas hemagglutinin-esterase fusion (HEF) proteins have been found to be the ... primary single spike protein that combines all of the enzymatic activities listed above. HEF proteins have been tested to be ...
Müller R, Thiele J, Klages U, Lingens F (October 1984). "Incorporation of [18O]water into 4-hydroxybenzoic acid in the reaction ... Heppel LA, Porterfield VT (November 1948). "Enzymatic dehalogenation of certain brominated and chlorinated compounds". The ...
The oxygen incorporated need not be derived from O2, with NADH or NADPH as one donor, and incorporation of one atom of oxygen ... This enzymatic activity is now known to be performed by the product of the CYP8B1 gene. This step leads to the formation of the ... with O2 as oxidant and incorporation or reduction of oxygen. ...
The peptide was eventually determined to be 39 amino acids in length, however enzymatic and mild acid cleavage suggested that a ... apparent from the outset that ACTH contained the amino acid Arginine and thus methods had to be developed for the incorporation ... full enzymatic activity was restored. Hofmann speculated that this system might be a model for the way peptide hormones ...
This enzymatic activity can be measured with high time resolution in real time. The material consumption of the all optical MST ... Radiometric assays measure the incorporation of radioactivity into substrates or its release from substrates. The radioactive ... ISBN 0-89573-236-X. Passonneau, J.V.; Lowry, O.H. (1993). Enzymatic Analysis. A Practical Guide. Totowa NJ: Humana Press. pp. ... Enzyme assays are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and ...
Enzymatic cleavage of the pregnane side chain". The Journal of Biological Chemistry. 241 (15): 3615-23. PMID 5950688. Portal: ... with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O miscellaneous.[ ...
Prairie RL, Talalay P (1963). "Enzymatic formation of testololactone". Biochemistry. 2: 203-8. doi:10.1021/bi00901a039. PMID ... with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derive from O miscellaneous. ...
SARS-CoV-2 RdRp has lower enzymatic activity and lower thermal stability compared to the RdRp domain in SARS-CoV. Nsp12 is ... Remdesivir is a nucleoside analog which can compete with ATP for incorporation into the RNA strand and prematurely terminate ...
The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms of oxygen ... I Enzymatic formation of catechol from benzene". Biochemistry. 7 (7): 2653-62. doi:10.1021/bi00847a031. PMID 4298226. Portal: ... with O2 as oxidant and incorporation or reduction of oxygen. ...
... the low membrane permeability and vulnerability to enzymatic degradation of endomorphins limits their incorporation into drugs ...
This PP-fold has been found to protect the peptide against enzymatic attack as well as producing a hydrophobic pocket which is ... The PP-fold is formed through the incorporation of certain residues which are predominately Pro2, Pro5, Pro8, Gly9, Tyr20 and ... Peptide YY cannot be produced as the result of enzymatic breakdown of crude fish proteins and ingested as a food product. ...
The formation of sulfate in aerobic conditions entails the incorporation of four oxygen atoms from water, and when coupled with ... enzymatic processes usually discriminate against the heavy isotopes, and, as a consequence, biological fractionations of ... suggest a kinetic isotope effect in the incorporation of oxygen from water to sulfate and the role of nitrate as a potential ... the isotopic fractionation associated with the incorporation of oxygen atoms from water to sulfate and a potential exchange of ...
... deletion or mis-incorporation. Enzymatic DNA editing Cytidine deaminase enzymes: This family of enzymes are part of the innate ... Direct DNA oxidation damage leads to the creation of 8-Oxoguanine, which if remains un-repaired, will lead to incorporation of ... APOBEC3 family of cytidine deaminase enzymes respond to viral infections by editing viral genome, but the enzymatic activity of ... and DNA enzymatic editing. The term is used for two distinct concepts, often conflated: mutagen signatures and tumor signatures ...
... incorporation of nucleotides using an engineered polymerase and (4) detection of the incorporation of nucleotide. Then steps 3- ... The 3' blocking groups were originally conceived as either enzymatic or chemical reversal The chemical method has been the ... An engineered polymerase is used to synthesize a copy of a single strand of DNA and the incorporation of each nucleotide is ... A fluorescently-labeled terminator is imaged as each dNTP is added and then cleaved to allow incorporation of the next base. ...