Loading...
*  Gábor Vajta
... a new tool for in vitro embryo culture: A technique report". Theriogenology. 48 (8): 1379. doi:10.1016/S0093-691X(97)00379-8. ... "New method for culture of zona-included or zona-free embryos: The Well of the Well (WOW) system". Molecular Reproduction and ... US20090119787 "Futi on Embryo Plus" "Futi on BBC" "Danish piglets" Kragh, P. M.; Nielsen, A. L.; Li, J.; Du, Y.; Lin, L.; ... The technique does not require micromanipulators only a simple stereomicroscope and an inexpensive fusion machine. All work is ...
*  New Rice for Africa
Because the different species do not naturally interbreed, a plant tissue culture technique called embryo-rescue was used to ...
*  Triticale
To improve the viability of the embryo and thus avoid its abortion, in vitro culture techniques were developed (Laibach, 1925)[ ... The in vitro culture of anthers and microspores is most often used in cereals, including triticale. These two techniques are ... Tissue culture techniques with respect to wheat and triticale have seen continuous improvements, but the isolation and ... This technique is applied rather extensively to wheat. Its success is in large part due to the insensitivity of maize pollen to ...
*  Embryo rescue
Ovule culture or in ovolo embryo culture is a modified technique of embryo rescue whereby embryos are cultured while still ... The most widely used embryo rescue procedure is referred to as embryo culture, and involves excising plant embryos and placing ... Embryo rescue is one of the earliest and successful forms of in-vitro culture techniques that is used to assist in the ... This technique nurtures the immature or weak embryo, thus allowing it the chance to survive. Plant embryos are multicellular ...
*  Grape
... must either use a seeded variety as the female parent or rescue embryos early in development using tissue culture techniques. ... The earliest archeological evidence for a dominant position of wine-making in human culture dates from 8,000 years ago in ...
*  Stenospermocarpy
Grape breeders have developed some new seedless grape cultivars by using the embryo rescue technique. Before the tiny embryo ... aborts, it is removed from the developing fruit and grown in tissue culture until it is large enough to survive on its own. ... Embryo rescue allows the crossing of two seedless grape cultivars. Seedless grapes are divided into white, red and black types ... however subsequent abortion of the embryo that began growing following fertilization leads to a near seedless condition. The ...
*  Jacques Cohen
1992). "Evaluation of Vero cell co-culture system for mouse embryos in various media". Human Reproduction. 7 (2): 276-80. PMID ... Cohen is known for the application of micromanipulation techniques to operate on eggs, sperm and embryos. Intracytoplasmic ... He also co-cultured human embryos with helper cells to promote growth. ... He developed a precursor technique of Intracytoplasmic Sperm Injection (ICSI), which is now used for treatment of nearly all ...
*  Embryonic stem cell
... revealing a new technique for culturing the mouse embryos in the uterus to allow for an increase in cell number, allowing for ... After 4-6 days of this intrauterine culture, the embryos are harvested and grown in in vitro culture until the inner cell mass ... She showed that embryos could be cultured in vitro and that ES cells could be derived from these embryos. In 1998, a ... She removed the embryos from the donor mother at approximately 76 hours after copulation and cultured them overnight in a ...
*  List of MeSH codes (E05)
... embryo culture techniques MeSH E05.200.249.484 --- organ culture techniques MeSH E05.200.249.617 --- tissue culture techniques ... cell culture techniques MeSH E05.200.249.374 --- coculture techniques MeSH E05.200.249.437 --- diffusion chambers, culture MeSH ... patch-clamp techniques MeSH E05.200.750.132 --- autoradiography MeSH E05.200.750.210 --- bone demineralization technique MeSH ... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 --- fluorescent antibody technique, direct MeSH E05.200.750.551 ...
*  Shell-less chick embryo culture
Using this technique, observations can be made, whether it is a induced-malformation caused due to effect of teratogens or the ... Shell-less chick embryo culture is the process of growing chick embryos in vitro, without their protective egg shells, for ... Tahara Y, Obara K. (2014). "A novel shell-less culture system for chick embryos using a plastic film as culture vessels". The ... Chick embryos and other avian embryos have been biological models to visualize the developmental stages of embryos for ...
*  Gladwyne, Pennsylvania
... pioneer in the development of techniques for manipulating the cellular and genetic composition of early mouse embryos and ... creator of the Brinster cell culture medium John Thompson Dorrance - American chemist who invented condensed soup; president of ...
*  Ralph L. Brinster
Brinster developed the first reliable in vitro culture system for early mammalian embryos. These techniques have been conserved ... During the 1960s, Brinster pioneered the development of techniques to manipulate mouse embryos-his techniques have made the ... This "Brinster Method" of embryo manipulation is so ubiquitous in modern biology that other scientists rarely cite the work in ... 2003 Wolf Prize in Medicine, Israel "for the development of procedures to manipulate mouse ova and embryos, which has enabled ...
*  Statutory interpretation
e.g. does the use of a new cloning technique create an embryo within the meaning of statute enacted when embryos could only be ... and new technologies and cultures make application of existing laws difficult. ( ...
*  Gastrulation
However, it is important to relate the observations in culture to the processes occurring in the embryo for context. To ... culture techniques using Embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs). These are associated with ... Before gastrulation, the embryo is a continuous epithelial sheet of cells; by the end of gastrulation, the embryo has begun ... The proximal-distal axis is formed when the cells of the embryo form the "egg cylinder," which consists of the extraembryonic ...
*  Fate mapping
Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured in Vitro. ... later modifications of this technique employed DNase or the ricin A chain. The single-cell injection technique is now also in ... Also, embryos had to be fixed in order to stain for the HRP, thus allowing only a single timepoint view of each individual ... Injected embryos must also be kept in the dark to avoid non-specific uncaging from ambient light. Aside from chemical tracers, ...
*  Human cloning
... and the hybrid cell was cultured, and developed into an embryo. The embryo was destroyed after 12 days. In 2004 and 2005, Hwang ... Work on cloning techniques has advanced our basic understanding of developmental biology in humans. Observing human pluripotent ... The embryos were developed only to the blastocyst stage, at which point they were studied in processes that destroyed them. ... Human embryos and stem cells are also permitted to be donated for research.[citation needed] There have been consistent calls ...
*  Beatrice Mintz
She developed a technique that involved mixing cells from a black mouse strain into the blastocysts of white or brown mice in ... The resultant mice developed normally and their tissues were a mixture of cells derived from the two donor embryos. Mintz went ... These experiments, which took eight years, utilized some of the first pluripotent stem cell cultures ever made. Mintz and ... Using these techniques Mintz was able to establish the genetic basis of certain kinds of cancer and in 1993 she produced the ...
*  Chemical imaging
Novel Techniques for Microspectroscopy and Chemical Imaging Analysis of Soybean Seeds and Embryos.(2002). Baianu, I.C., ... Chemical Imaging and NMR Analysis of Oil in Developing and Mutagenized Soybean Embryos in Culture. (2003). Baianu, I.C., ... Many imaging techniques can be used to analyze samples of all sizes, from the single molecule to the cellular level in biology ... Chemical imaging techniques are critical to understanding modern manufactured products and in some cases is a non-destructive ...
*  Tissue typing
One technique of tissue typing, "mixed leukocyte reaction", is performed by culturing lymphocytes from the donor together with ... An embryo can be tissue typed to ensure that the embryo implanted can be a cord-blood stem cell donor for a sick sibling. ... In this technique a donor's blood cells are MHC typed by mixing them with serum containing the anti-HLA antibodies. If the ... Another technique, known as a micro-cytotoxicity assay, utilizes serum with known anti-HLA antibodies that recognize particular ...
*  Gerald Fischbach
However, cultures containing isolated spinal cord cells were unable to send similar action potentials. This technique proved to ... When ARIA isolated from chick embryo brain was applied to chicken myotubes, which are developing chicken muscle fibers, it was ... Fischbach used this technique to reconstruct neuromuscular junctions from dissociated spinal cord and muscle cells from chick ... However, when wild-type neuregulin was added to homozygous mutant neuregulin explants (isolated tissue cell cultures) nine days ...
*  Montrose Thomas Burrows
These improvements include the substitution of blood plasma for lymph and the addition of embryo extract in the tissue culture ... One significant innovation was the method they developed for serial activation of tissues in culture. This technique ... Their batch of cultures lasted from 1912 to 1946. Because the tissue cultures far outlived their organisms, they were deemed " ... Jang, Liling, "Alexis Carrel's Immortal Chick Heart Tissue Cultures (1912-1946)," The Embryo Project Encyclopedia Burrows, ...
*  Preimplantation genetic diagnosis
The technique can be used for prenatal sex discernment of the embryo, and thus potentially can be used to select embryos of one ... This implies that the patient's oocytes should be fertilized in vitro and the embryos kept in culture until the diagnosis is ... The latter technique has proved to be less deletereous for the embryo, therefore it is advisable to perform the biopsy around ... Embryo transfer is usually performed on day three or day five post-fertilization, the timing depending on the techniques used ...
*  Plant breeding
There are many plant tissue culturing techniques that can be used to achieve the haploid plants, but microspore culturing is ... The classical plant breeder may also make use of a number of in vitro techniques such as protoplast fusion, embryo rescue or ... Such a method is referred to as Embryo Rescue. This technique has been used to produce new rice for Africa, an interspecific ... When distantly related species are crossed, plant breeders make use of a number of plant tissue culture techniques to produce ...
*  Organ culture
The media used for a growing organ culture are generally the same as those used for tissue culture. The techniques for organ ... They then introduced human stem cells inside the pig embryo to have the human DNA fill in the gaps. Embryonic organ culture is ... The following are four techniques employed for embryonic organ culture. The following are general steps in organ culture on ... Organ culture is a development from tissue culture methods of research, the organ culture is able to accurately model functions ...
*  Cell culture
... Basics - Introduction to cell culture, covering topics such as laboratory set-up, safety and aseptic technique ... It also offers to confirm single cell origin of somatic embryos and the asymmetry of the first cell division, which starts the ... Plant cell cultures are typically grown as cell suspension cultures in a liquid medium or as callus cultures on a solid medium ... These are generally performed using tissue culture methods that rely on aseptic technique. Aseptic technique aims to avoid ...
*  Paul J. Turek
This technique was subsequently applied to men with genetically immotile sperm with success and this technique is now used ... for men in the future without the need for embryos and all of the political and ethical issues that the use of human embryos ... embryonic-like stem cells when placed in an appropriate culture environment. This finding has been independently confirmed by ... This technique has been important because it has improved identification of men who are likely to have a successful sperm ...