Electroporation. Early versions of electroporation used bulk electrodes to apply electrical pulses of defined voltage to cells ... The nano and micro versions of electroporation feature much higher precision and control over the size and location of membrane ... By performing intracellular delivery with electroporation, bleomycin potency can be increased more than a hundred-fold . As for ... Patino, C (2022). "Multiplexed high-throughput localized electroporation workflow with deep learning-based analysis for cell ...

Takei Y (2014). "Electroporation-mediated siRNA delivery into tumors". Electroporation Protocols. Methods in Molecular Biology ... For cells that are difficult to transfect electroporation is advantageous however cell death is more probable under this ... delivery into bovine monocyte-derived macrophages by transfection and electroporation". Veterinary Immunology and ...

Electroporation is a method that uses high voltage to create pores in the membranes of mammalian cells. By pulsing with ... Spencer, Sarah C. (1991), Murray, E. J. (ed.), "Electroporation Technique of DNA Transfection", Gene Transfer and Expression ... "Electroporation-Mediated Gene Delivery". Advances in Genetics. 89: 49-88. doi:10.1016/bs.adgen.2014.10.003. ISBN 9780128022726 ...

S. Roberts, "Electroporation: galvanizing cells into action," ''J. NIH Res.,'' vol. 2, pp. 93-94, 1990. Li, C. J.; Heim, R.; Lu ... This is called "electroporation". Chang invented a technique using a pulsed radio frequency electric field to achieve the ... In: Neumann E., Sowers A.E., Jordan C.A. (eds) Electroporation and Electrofusion in Cell Biology. Springer, Boston, MA Guide to ... Chang, D. C.; Reese, T. S. (1990). "Changes in membrane structure induced by electroporation as revealed by rapid-freezing ...

Because of this, electroporation is one of the key methods of transfection as well as bacterial transformation. It has even ... In contrast, during electroporation the lipid molecules are not chemically altered but simply shift position, opening up a pore ... Electroporation is the rapid increase in bilayer permeability induced by the application of a large artificial electric field ... Experimentally, electroporation is used to introduce hydrophilic molecules into cells. It is a particularly useful technique ...

Chen C, Smye SW, Robinson MP, Evans JA (March 2006). "Membrane electroporation theories: a review". Med Biol Eng Comput. 44 (1- ... August 2008). "The effect of electroporation pulses on functioning of the heart". Med Biol Eng Comput. 46 (8): 745-57. doi: ... Weaver, J.C. (February 2000). "Electroporation of cells and tissues" (PDF). IEEE Transactions on Plasma Science. 28 (1): 24-33 ... This phenomenon is called electroporation (or electropermeabilization) and is becoming widely used to improve anticancer drug ...

"Inovio's License of Electroporation Intellectual Property Includes Skin Delivery Technology Used in Anthrax Collaboration". ... "Electroporation device and injection apparatus". USPTO. US7328064B2. "Proprietary smart devices (Cellectra)". Inovio ... intratumoral and intramuscular electroporation... The ex-vivo patents are relevant to the delivery of genes to dendritic and ...

Weaver JC, Chizmadzhev YA (December 1996). "Theory of electroporation: a review". Bioelectrochemistry and Bioenergetics. 41 (2 ... parameter in biological phenomena as it regulates the self-healing properties of the bilayer following electroporation or ...

A high-efficiency electroporation protocol for S.punctatus and two related chytrids species B. dendrobatidis and B. ... Electroporation using unsynchronized zoospores can also reach more than 80% efficiency. S.punctatus globular zoospores (3-5 mm ... Swafford AJ, Hussey SP, Fritz-Laylin LK (September 2020). "High-efficiency electroporation of chytrid fungi". Scientific ...

The bacteria is able to uptake a free plasmid using electroporation and the plasmid material will integrate into the host DNA ... Mack, K.; Titball, R. W. (1996-11-01). "Transformation of Burkholderia pseudomallei by electroporation". Analytical ... which showed that a Burkholderia pseudomallei 4845 was capable of transformation by electroporation, utilizing the incQ plasmid ...

014103 (2009) 4. Electroporation : J.K. Valley, et al., Lab Chip doi:10.1039/b821678a (2009) 5. Local Chemical Concentration ...

Electroporation is a method of promoting competence. Cells are briefly shocked with an electric field of 10-20 kV/cm, which is ... Due to the damage caused to the cells and DNA the transformation efficiency of biolistics and electroporation is lower than ... Plants cells can also be transformed using electroporation, which uses an electric shock to make the cell membrane permeable to ...

1989). Electroporation and Electrofusion in Cell Biology. Springer. ISBN 978-0-306-43043-5.[page needed] Malle, M.G.; Löffler, ... Fusion can also be artificially induced through electroporation in a process known as electrofusion. It is believed that this ... phenomenon results from the energetically active edges formed during electroporation, which can act as the local defect point ...

Electroporation is another method of promoting competence. In this method the cells are briefly shocked with an electric field ... Cells used in electroporation should be prepared first by washing in cold double-distilled water to remove charged particles ... Electroporation: Formation of transient holes in the cell membranes using electric shock; this allows DNA to enter as described ... Electroporation: Formation of transient holes in cell membranes using electric pulses of high field strength; this allows DNA ...

Damage due to current is through tissue heating and/or electroporation injury. For most cases of high-energy electrical trauma ... Andrei G. Pakhomov; Damijan Miklavcic; Marko S. Markov (2 June 2010). Advanced Electroporation Techniques in Biology and ... for example nerve conduction studies and electromyography Electroporation for gene delivery Mild electric shocks are also used ...

Bulk electroporation (BEP) is the most conventional electroporation method. Benefits come in the form of high throughput and ... Attempts have been made to miniaturize electroporation such as microelectroporation (MEP) and nanochannel electroporation (NEP ... Electroporation is a physical method which harnesses an electric field to open pores in the normally semi-permeable cell ... Shi J, Ma Y, Zhu J, Chen Y, Sun Y, Yao Y, Yang Z, Xie J (November 2018). "A Review on Electroporation-Based Intracellular ...

Electroporation is another method of promoting competence. In this method the cells are briefly shocked with an electric field ... Stressing the bacteria with a heat shock or electroporation can make the cell membrane permeable to DNA that may then be ... Due to the damage caused to the cells and DNA the transformation efficiency of biolistics and electroporation is lower than ... Plants cells can also be transformed using electroporation, which uses an electric shock to make the cell membrane permeable to ...

Irreversible electroporation video demonstration: Irreversible Electroporation (NanoKnife®) to Treat Prostate Tumors , Memorial ... Martin, Robert C. G. (June 2015). "Use of irreversible electroporation in unresectable pancreatic cancer". Hepatobiliary ... irreversible electroporation (IRE)) or freeze (cryoablation) solid tumors resulting in tumor cell death. Ablation techniques ... Microwave Ablation Used To Treat Cancer Quickly at Stanford Irreversible electroporation video demonstration: Stony Brook ...

"Intracellular recording of action potentials by nanopillar electroporation". Nature Nanotechnology. 7 (3): 185-190. Bibcode: ...

"Optimization of electroporation conditions for toyocamycin producer 1628". Journal of Basic Microbiology. 54 (4): 278-284. doi: ...

This can be assisted either 1) by electroporation; 2) by temporarily damaging muscle fibres with myotoxins such as bupivacaine ... "Increased DNA vaccine delivery and immunogenicity by electroporation in vivo". Journal of Immunology. 164 (9): 4635-40. doi: ...

Martin, Robert C. G. (June 2015). "Use of irreversible electroporation in unresectable pancreatic cancer". Hepatobiliary ... Irreversible electroporation video demonstration: https://www.mskcc.org/videos/irreversible-electroporation-nanoknife-treat- ... irreversible electroporation(IRE)) or freeze (cryoablation) solid tumors resulting in tumor cell death. Ablation techniques can ... inoperable tumors can be treated with image guided ablative techniques and more recently irreversible electroporation. ...

"Transfer of monoclonal antibodies into mammalian cells by electroporation". Journal of Biological Chemistry. 264 (26): 15494- ...

Cerda GA, Thomas JE, Allende ML, Karlstrom RO, Palma V (July 2006). "Electroporation of DNA, RNA, and morpholinos into ... Jubin R (2004). "Optimizing electroporation conditions for intracellular delivery of Morpholino antisense oligonucleotides ... electroporation, or scrape loading. Delivery into adult tissues is usually difficult, though there are a few systems allowing ... an alternative method for embryonic delivery is electroporation, which can deliver oligos into tissues of later embryonic ...

Mazy-Servais, C (15 June 1997). "Electroporation of intact cells of Streptomyces parvulus and Streptomyces vinaceus". FEMS ...

Electroporation is a method that uses short pulses of high voltage to carry DNA across the cell membrane. This shock is thought ... Electroporation is generally efficient and works across a broad range of cell types. However, a high rate of cell death ... Compared to electroporation, the technique resulted in greatly increased efficiency and less cellular damage. The use of ... More recently a newer method of electroporation, termed electron-avalanche transfection, has been used in gene therapy ...

Mazy-Servais, Cécile; Baczkowski, Dominique; Dusart, Jean (17 January 2006). "Electroporation of intact cells of Streptomyces ...

... such as electroporation. These methods are incapable of targeting specific cells, and are therefore not usable where efficiency ...

The plasmids were then transformed into M. tuberculosis cells by electroporation. Colonies were plated on kanamycin to select ...

Some non-viral methods are detailed below: Electroporation is a technique in which high voltage pulses are used to carry DNA ... Lambricht L, Lopes A, Kos S, Sersa G, Préat V, Vandermeulen G (2015). "Clinical potential of electroporation for gene therapy ... electroporation results in a high rate of cell death which has limited its use. An improved version of this technology, ...