... drug screening assays, antitumor MeSH E05.337.550.200.800 - tumor stem cell assay MeSH E05.337.550.200.900 - xenograft model ... drug screening assays, antitumor MeSH E05.200.500.388.930 - tumor stem cell assay MeSH E05.200.500.410 - electroporation MeSH ... multiphasic screening MeSH E05.318.308.250.580.580 - neonatal screening MeSH E05.318.308.250.580.925 - vision screening MeSH ... antitumor assays MeSH E05.337.550.200.900.830 - subrenal capsule assay MeSH E05.337.550.400 - microbial sensitivity tests MeSH ...

These isolated molecules are screened via in-vitro assays to measure their effects in standardized paradigms designed to select ... Many efforts to discover cancer therapeutic drugs focus on the screening of novel biomolecules produced and isolated from ... Kalkitoxin was originally isolated from Lyngbya majuscula as an effort to collect new molecules for testing as antitumor or ... One of the first tests of kalkitoxin tumor-selective cytotoxicity used an in-vitro assay to test solid tumor selectivity of ...

Studies related to antitumor antibiotics. Part V. Reactions of mitomycin C with DNA examined by ethidium fluorescence assay. ... Microbial drug discovery: 80 years of progress. J Antibiot. 62:5-16. Lown JW, Begetter A, Johnson D, Morgan AR. 1976. ... Research on Antibiotic Screening in Japan over the Last Decade: A Producing Microorganisms Approach. Actinomycetologica. 9:100- ... Many natural compounds have led to the discovery of drugs used to treat human disease. Out of the 22,500 biologically active ...

Using a line of CD4+ T cells that they had made, they developed an assay to screen drugs for their ability to protect CD4+ T ... leading to the development of the antitumor agent 6-mercaptopurine. Richard E. Beltz first synthesized AZT in 1961, but did not ... Drugs with non-standard legal status, Articles with changed EBI identifier, ECHA InfoCard ID from Wikidata, Drug has EMA link, ... This assay could simultaneously test both the anti-HIV effect of the compounds and their toxicity against infected T cells. In ...

It enhances antitumor activity of some other drugs such as vincristine. Dactolisib seems to inhibit effectively both wild-type ... New mTOR-specific inhibitors came forth from screening and drug discovery efforts. These compounds block activity of both mTOR ... Thus, this data is based on preclinical assays, based on in vitro cultured tumor cell lines, which suggest that the effects of ... Compared with drugs that inhibit either mTORC1 or PI3K, these drugs have the benefit of inhibiting mTORC1, mTORC2, and all the ...

October 2019). "Performance of Interferon-Gamma Release Assays for Tuberculosis Screening in Pediatric Inflammatory Bowel ... Many 5-ASA drugs have been developed with the aim of delivering the active compound to the large intestine to maintain ... July 2018). "A Prospective Study to Monitor for Tuberculosis During Anti-tumour Necrosis Factor Therapy in Patients With ... A series of drugs in development looks to disrupt the inflammation process by selectively targeting an ion channel in the ...

Applying the revised screen led to the discovery of the potent biological activity of ciclosporin on 31 January 1972. This drug ... Guided by Stähelin's in-vitro and in-vivo assays, they found the active compound responsible for the good antitumor activity of ... Stähelin's invention of this procedure initiated an innovative screening system for discovering immunosuppressant drugs lacking ... This screening process was first used in the pharmacology department in January 1970, before Jean F. Borel joined Stähelin's ...

2007). "Preclinical antitumor activity of a novel folate-targeted dual drug conjugate". Mol. Pharm. 4 (5): 659-67. doi:10.1021/ ... Because novel FR-targeted therapies are now being tested clinically, having the ability to screen patients for FR-positive ... These include an invasive tissue-based immunohistochemical assay, and a non-invasive radiodiagnostic approach. The latter ... Targeted drug therapy is advantageous because it deposits the drug at the specific location where it can be most useful in ...

October 2014). "In situ drug-receptor binding kinetics in single cells: a quantitative label-free study of anti-tumor drug ... Many ligand binding assays require a filtration step to separate bound and unbound ligands before screening. A method called ... 2009). Ligand-binding assays development, validation, and implementation in the drug development arena. Hoboken, N.J.: John ... A ligand binding assay (LBA) is an assay, or an analytic procedure, which relies on the binding of ligand molecules to ...

It was discovered in 1966 by M. E. Wall and M. C. Wani in systematic screening of natural products for anticancer drugs. It was ... The C- and D-rings have an essential role in the antitumor activity. Replacement in any position results in much less potent ... compound than parent compound in other cytotoxicity assay. The E-ring doesn't allow many structural changes without losing CPT ... Reduced drug-HSA interactions could result in improved activity. Studies have shown that substitution at position 7, 9, 10 and ...

These reagents are therefore important for future drug and vaccine development. Immudex developed a CMV Dextramer® assay for ... MHC tetramers are used in studies of pathogen immunity and vaccine development, in evaluation of antitumor responses, in ... covering a broad range of epitopes to assist with screening and monitoring CMV progression in future clinical settings. MHC ... Tetramer assays are used for single-cell phenotyping and cell counting, and offer an important advantage over other methods, ...

"Such cell cultures can lead to new insights into cell and organ function and be used for drug screening". A kidney-on-a-chip ... October 2018). "Live-cell phenotypic-biomarker microfluidic assay for the risk stratification of cancer patients via machine ... based biomimetic liver tumor-on-a-chip proved to be a suitable design for further anti-tumor studies. Zhou et al. analyzed ... Fan Y, Nguyen DT, Akay Y, Xu F, Akay M (May 2016). "Engineering a Brain Cancer Chip for High-throughput Drug Screening". ...

In vitro assays have shown them both to be selective AR agonists and that they inhibit proliferation of several prostate cancer ... Screening of chemical libraries for AR blockers led to the discovery of the first antiandrogen, cyproterone. An acetate group ... These drugs may therefore be effective as a second-line therapy for refractory prostate cancer previously treated with ... "Antitumour activity of MDV3100 in castration-resistant prostate cancer: a phase 1-2 study". Lancet. 375 (9724): 1437-46. doi: ...

"In vitro screening for inhibitors of the human mitotic kinesin Eg5 with antimitotic and antitumor activities". Mol Cancer Ther ... microtubule gliding assays, single molecule motility assays, and optical trap assays. In microtubule gliding assays, kinesins ... Drugs that specifically inhibit only human Kinesin-5 are alternatives to the taxanes and vinc alkaloids that target ... In single molecule motility assays, velocities for Kinesin-5 were similar to those seen in microtubule gliding assays, and the ...

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Additional screens ... Yeast three-hybrid assays have shown that this protein binds to the stem loops within the 3'UTR of the POLB mRNA, however the ... and drug resistance. The mitochondrial DNA of mammalian cells is constantly under attack from oxygen radicals released during ... impact on sensitivity towards antitumor agents". Oncogene. 20 (43): 6181-7. doi:10.1038/sj.onc.1204743. PMID 11593426. ...

... rationale and design of screening assays, and the application of such assays for screening of natural product extracts and the ... Antisense drugs are based on the fact that antisense RNA hybridizes with and inactivates mRNA. These drugs are short sequences ... SLR14 improved antitumor efficacy of anti-PD1 antibody over single-agent treatment. SLR14 was absorbed by CD11b+ myeloid cells ... Sahin U, Karikó K, Türeci Ö (October 2014). "mRNA-based therapeutics--developing a new class of drugs". Nature Reviews. Drug ...

... technical barriers to screening natural products in high-throughput assays]. The growing appreciation of functional assays and ... One example of successful use of this strategy is the screening for antitumor agents by the National Cancer Institute, which ... It is unlikely that a perfect drug candidate will emerge from these early screening runs. One of the first steps is to screen ... Zheng W, Thorne N, McKew JC (November 2013). "Phenotypic screens as a renewed approach for drug discovery". Drug Discovery ...

When anticancer drugs such as doxorubicin accumulate in microvesicles, the drug's cellular levels decrease. This can ultimately ... Several tumor markers accessible as proteins in blood or urine have been used to screen and diagnose various types of cancer. ... Circulating microvesicles isolated from cardiac surgery patients were found to be thrombogenic in both in vitro assays and in ... Like other EVs, they have been implicated in numerous physiologic processes, including anti-tumor effects, tumor immune ...

Can it be a Multifunctional Drug?". Current Drug Metabolism. 21 (7): 512-524. doi:10.2174/1389200221666200610172105. PMID ... Some screening protocols are currently not accurate (such as prostate-specific antigen testing). Others such as a colonoscopy ... are assayed on cultures of bacteria and mammalian cells. The goal of oncogenomics is to identify new oncogenes or tumor ... "Bifunctional cancer cell-based vaccine concomitantly drives direct tumor killing and antitumor immunity". Science Translational ...

... so researchers collect samples from around the world to analyze and evaluate in drug discovery screens or bioassays. This ... June 2020). Assay Guidance Manual. Bethesda: Eli Lilly & Company and the National Center for Advancing Translational Sciences. ... Other natural products derived from marine animals and under investigation as possible therapies include the antitumour agents ... Drug Discovery - Is Mother Nature still the number one source for promising new drugs? Patrick GL (2013). "12.4.2: Medical ...

Boelsterli UA, Ho HK, Zhou S, Leow KY (October 2006). "Bioactivation and hepatotoxicity of nitroaromatic drugs". Current Drug ... February 2004). "Comparison of the Hershberger assay and androgen receptor binding assay of twelve chemicals". Toxicology. 195 ... "Effects of combined treatment with niftolide and low doses of antitumor estrogen preparation, chlorotrianizene, on rat prostate ... evidence for complex I inhibition and mitochondrial dysfunction using toxicogenomic screening". Chemical Research in Toxicology ...

"Abraxane Drug Information Archived 2005-05-26 at the Wayback Machine." Food and Drug Administration. January 7, 2005. Retrieved ... In general, in vitro assays involving microtubules, such as motility assays, rely on paclitaxel to maintain microtubule ... The discovery of paclitaxel began in 1962 as a result of a NCI-funded screening program. A number of years later it was ... Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT (May 1971). "Plant antitumor agents. VI. The isolation and structure of taxol ...

Quintavalle M, Elia L, Price JH, Heynen-Genel S, Courtneidge SA (July 2011). "A cell-based high-content screening assay reveals ... Cdk5 has been proven to be directly linked with drug abuse. We know that drugs act in the reward system, reaching their action ... Cdk5 is involved in tumor proliferation, migration, angiogenesis and also chemotherapy resistance and anti-tumor immunity. It ... Hence, its relation to drug abuse and more specifically to the reward system, which is triggered by the consumption of drugs. ...

2008). Analogous successful use of IMC to determine the effects of antitumor drugs on tumor cells in culture within a few hours ... If absolute data are required (e.g. quantity of product produced by a process), then assays can be conducted in parallel on ... This is especially useful for comparative screening-e.g. the effects of different combinations of material composition and/or ... Also, IMC not only documents the general metabolic decline over time due to the drugs, but also the overall frequency of worm ...

Ther Drug Monit. 36 (1): 93-99. doi:10.1097/FTD.0b013e3182a04fc7. PMID 24061446. S2CID 21072472. "ARK Methotrexate Assay". Ark ... DNA-Interactive Alkylating Agents and Antitumour Platinum-Based Drugs". The Cancer Handbook. John Wiley & Sons, Ltd. doi: ... and hence it has been recommended that the babies be screened. Between 30 and 40 percent of people undergoing chemotherapy ... drug-to-drug interactions, genetics, and obesity, which have major impacts on the actual concentration of the drug in the ...

... and drug screening applications. Using MitoBloCK-6 and/or PluriSIn # 1 the differentiated progenitor cells can be further ... iPSCs have been shown to support the development of all-iPSC mice using a tetraploid (4n) embryo, the most stringent assay for ... They augment anti-tumor responses by producing interferon-gamma (IFN-γ). The approach of collection, reprogramming/ ... of drugs, and toxicity testing of various drugs. The tissue grown from iPSCs, placed in the "chimeric" embryos in the early ...

... survivin was expressed in all 60 different human tumour lines used in the National Cancer Institute's cancer drug-screening ... In vitro assays and other tests were also performed to validate the idea of the occurrence of an actual immune response to ... "Survivin-derived peptide epitopes and their role for induction of antitumor immunity in hematological malignancies". Leuk. ... A. Cellular T cell response The first evidence of survivin-specific CTL recognition and killing was shown in an assay wherein ...

Some cationic drugs, such as chloroquine and sertraline, are known as lysosomotropic drugs. These drugs are weak bases that ... During a screen seeking to identify microbial secondary metabolites whose activity mimicked that of two cardiac glycosides, ... In a rabbit contractility assay, bafilomycin was used to pre-treat isolated rabbit aorta. The lipophilic agent xylometazoline, ... Bafilomycins exhibit a wide range of biological activity, including anti-tumor, anti-parasitic, immunosuppressant and anti- ...

Drugs Controller General of India is the head of department of the Central Drugs Standard Control Organization of the ... Pregnant women are often screened for continued resistance to rubella. The human papillomavirus vaccine is recommended in the U ... van Oirschot JT, Gielkens AL, Moormann RJ, Berns AJ (June 1990). "Marker vaccines, virus protein-specific antibody assays and ... "Prophylactic vaccines are potent activators of monocyte-derived dendritic cells and drive effective anti-tumor responses in ...

A number of similar analogues were found and assayed. Structurally similar series of c-Met inhibitors in which a phenolic hinge ... "Orphan Drug Designation". Merck KGaA, Darmstadt, Germany (Press release). 20 November 2019. Retrieved 8 November 2020. (CS1 ... "The discovery of benzanilides as c-Met receptor tyrosine kinase inhibitors by a directed screening approach", Bioorganic & ... offered the first proof that relatively selective c-Met inhibitors could be identified and that the inhibition leads to an anti ...