Some include: DNA sequencing; capillary electrophoresis; mass spectrometry; single-strand conformation polymorphism (SSCP); ... Haplotype mapping: sets of alleles or DNA sequences can be clustered so that a single SNP can identify many linked SNPs. ... Single nucleotide substitutions with an allele frequency of less than 1% are sometimes called single-nucleotide variants (SNVs ... An intronic SNP in DNA mismatch repair gene PMS2 (rs1059060, Ser775Asn) is associated with increased sperm DNA damage and risk ...

... and Single-Stranded DNA Polymers". Physical Review Letters. American Physical Society (APS). 92 (4): 048303. Bibcode:2004PhRvL ... Tang, Jau; Marcus, R. A. (2005-11-22). "Single particle versus ensemble average: From power-law intermittency of a single ... "Counting statistics of single molecule reaction events and reaction dynamics of a single molecule". Chemical Physics Letters. ... Colquhoun, D.; Hawkes, A. G. (1982-12-24). "On the Stochastic Properties of Bursts of Single Ion Channel Openings and of ...

Kikuchi M, Ohnishi K, Harayama S (2000-02-08). "An effective family shuffling method using single-stranded DNA". Gene. 243 (1 ... Ogiwara H, Kohno T (2011-12-14). "Essential factors for incompatible DNA end joining at chromosomal DNA double strand breaks in ... First, DNase I is used to fragment a set of parent genes into segments of double stranded DNA ranging from 10-50 bp to more ... In RACHITT, fragments of single stranded (ss) parent genes are annealed onto a ss template resulting in decreased mismatching ...

... sequences that melt become single-stranded and wash off. The temperatures at which labelled DNA comes off reflects the amount ... The mixture is incubated to allow DNA strands to dissociate and then cooled to form renewed hybrid double-stranded DNA. ... "DNA melting". To assess the melting profile of the hybridized DNA, the double-stranded DNA is bound to a column or filter and ... One method was introduced for hybridizing large numbers of DNA samples against large numbers of DNA probes on a single membrane ...

... single stranded DNA binding protein 3". Wu L (January 2006). "Structure and functional characterization of single-strand ... Single-stranded DNA-binding protein 3 is a protein that in humans is encoded by the SSBP3 gene. GRCh38: Ensembl release 89: ... Raval-Fernandes S, Kickhoefer VA, Rome LH (September 1999). "Cloning of a cDNA encoding a sequence-specific single-stranded-DNA ... evolutionarily conserved gene family with putative sequence-specific single-stranded DNA-binding activity". Genomics. 80 (1): ...

... is a family of single-stranded DNA viruses. The genomes of this family are small (2.3-2.8 kilobases in length). ... The name Smacoviridae stands for 'small circular genome virus'. The genomes are circular single-stranded DNA and encode rolling ... a Virus Phylum Unifying Seven Families of Rep-Encoding Viruses with Single-Stranded, Circular DNA Genomes". Journal of Virology ... a new family of animal-associated single-stranded DNA viruses". Archives of Virology. 163 (7): 2005-2015. doi:10.1007/s00705- ...

Single-stranded DNA-binding protein 2 is a protein that in humans is encoded by the SSBP2 gene. GRCh38: Ensembl release 89: ... "Entrez Gene: SSBP2 single-stranded DNA binding protein 2". Hu RM, Han ZG, Song HD, et al. (2000). "Gene expression profiling in ... 2007). "Adenoviral E1B55K oncoprotein sequesters candidate leukemia suppressor sequence-specific single-stranded DNA-binding ... evolutionarily conserved gene family with putative sequence-specific single-stranded DNA-binding activity". Genomics. 80 (1): ...

C. J. Chetsanga; V. Boyd; L. Peterson; K. Rushlow (1 January 1975). "Single-stranded regions in DNA of old mice" (PDF). Nature ... Chetsanga has discovered two enzymes involved in the repair of damaged DNA: firstly, formamidopyrimidine DNA glycosylase, which ... "Release of 7-methylguanine residues whose imidazole rings have been opened from damaged DNA by a DNA glycosylase from ... "Release of 7-methylguanine residues whose imidazole rings have been opened from damaged DNA by a DNA glycosylase from ...

Single-stranded DNA-binding protein, mitochondrial is a protein that in humans is encoded by the SSBP1 gene. SSBP 1 is a ... "Entrez Gene: SSBP1 single-stranded DNA binding protein 1". Tiranti V, Rocchi M, DiDonato S, Zeviani M (April 1993). "Cloning of ... Diez-Tascón C, Dodds KG, Crawford AM (December 2002). "Linkage mapping of the ovine single-stranded DNA-binding protein 1 ( ... Yang C, Curth U, Urbanke C, Kang C (February 1997). "Crystal structure of human mitochondrial single-stranded DNA binding ...

This is done to make DNA fragments in blunt ends. HaeIII is not effective for single stranded DNA cleavage. HaeIII has a ... Blakesley RW, Dodgson JB, Nes IF, Wells RD (October 1977). "Duplex regions in "single-stranded" phiX174 DNA are cleaved by a ... HaeIII cuts both strands of DNA in the same location, yielding restriction fragments with blunt ends. Heat denaturation occurs ... The enzyme's recognition site-the place where it cuts DNA molecules-is the GGCC nucleotide sequence which means it cleaves DNA ...

... es (+ssRNA viruses) are a group of related viruses that have positive-sense, single-stranded genomes ... Smith EC, Denison MR (5 December 2013). "Coronaviruses as DNA wannabes: a new model for the regulation of RNA virus replication ... Modrow S, Falke D, Truyen U, Schätzl H (2013). "Viruses with Single-Stranded, Positive-Sense RNA Genomes". Molecular Virology. ... Double-stranded RNA virus Negative-strand RNA virus Sense (molecular biology) "Current ICTV Taxonomy Release , ICTV". ictv. ...

... s bind to either RNA or single stranded DNA. The nucleic acid is bound in an extended conformation across one side of ... The type I KH domains have a three stranded beta-sheet where all three strands are anti-parallel. In the type II domain two of ... "Nucleic acid base-to-protein aromatic side chain stacking interactions which are prevalent in other types of single stranded ... While type I domains are usually found in multiple copies within proteins, the type II are typically found in a single copy per ...

"Breaking and joining single-stranded DNA: the HUH endonuclease superfamily". Nature Reviews Microbiology. Nature Portfolio. 11 ... However they do find it to be highly conserved, 98% of successfully infected plants testing positive for Clink DNA. Timchenko ...

Unlike the diagram, the probe binds to single stranded DNA.) TaqMan probes can be conjugated to a minor groove binder (MGB) ... As the Taq polymerase extends the primer and synthesizes the nascent strand (from the single-stranded template), the 5' to 3' ... TaqMan probes are designed such that they anneal within a DNA region amplified by a specific set of primers. ( ... Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures". Nucleic Acids Res. 28 (2): 655-661 ...

DNA polymerase turns single-stranded DNA into double-stranded DNA. Helicases separate double strands of nucleic acids prior to ... cyclically driving it from B-DNA to A-DNA and back again. A-DNA is 23% shorter than B-DNA, and the DNA shrink/expand cycle is ... transitions between A-DNA and B-DNA provide the driving force for genome packaging in double-stranded DNA bacteriophages". ... Viral DNA packaging motors inject viral genomic DNA into capsids as part of their replication cycle, packing it very tightly. ...

A single strand of DNA, called the T-strand, is cut at nic by an enzyme called relaxase. This single strand is eventually ... single-stranded nicks in DNA in vitro and then adding the nicked DNA to an environment rich in DNA polymerase and tagged ... daughter strand) from the template strand (parental strand). DNA mismatch repair (MMR) is an important DNA repair system that ... The DNA polymerase then replaces the DNA nucleotides with the tagged ones, starting at the site of the single-stranded nick. ...

The helicase is a monomeric translocase and utilizes ATP to unwind DNA. The preferred substrates are single-stranded DNA ... as the mechanism of translocation on single-stranded DNA. A Mexican-wave model has been proposed based on the changes in ... It catalyzes the unwinding of double-stranded plasmid DNA that has been nicked at the replication origin by the replication ... "DNA helicase activity of PcrA is not required for the displacement of RecA protein from DNA or inhibition of RecA-mediated ...

"Super-resolution mbPAINT for optical localization of single-stranded DNA". ACS Applied Materials & Interfaces. 5 (19): 9338-43 ... where the dynamic binding and unbinding of a dye-labeled DNA probe to a fixed DNA origami is used to achieve stochastic single- ... Pyle JR, Chen J (2 November 2017). "Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging". Beilstein ... Shah, Shalin; Dubey, Abhishek K.; Reif, John (10 April 2019). "Programming Temporal DNA Barcodes for Single-Molecule ...

These oxygenated metabolites bind to double-stranded and single-stranded. Empirical data show that MC tends to bind mostly to G ... When injected in lung, kidney or liver tissue of rats, it appears that the liver is able to reverse the MC-binding to DNA. Lung ... A single ip injection (in inducible fetal lung supernatants) of 100 mg/kg of 3-MC to the mothers resulted in a maximal 50-fold ... To be carcinogenic, the MC metabolite has to be covalently bound to DNA. Therefore, it is necessary for MC to be oxygenated in ...

It binds to both single-stranded and double-stranded DNA, making contact with G residues in the purine-rich strand of its ... "The HeLa Pur factor binds single-stranded DNA at a specific element conserved in gene flanking regions and origins of DNA ... Kobayashi S, Agui K, Kamo S, Li Y, Anzai K (October 2000). "Neural BC1 RNA associates with pur alpha, a single-stranded DNA and ... Pur-alpha may act in ALS directly by binding this DNA repeat expansion or its single-stranded RNA transcript. One potential ...

gp2.5 has similar function to single-stranded DNA binding protein. gp2.5 protects single-stranded DNA produced during ... T7 DNA polymerase possesses a 3'-5' single and double stranded DNA exonuclease activity. This exonuclease activity is activated ... During this process, the DNA polymerase "reads" existing DNA strands and creates two new strands that match the existing ones. ... and single-stranded DNA-binding protein gp2.5. Although these three proteins are the only ones required for template single- ...

The viral genome is single-stranded deoxyribonucleic acid (DNA) with a molecular weight of 1.4 × 106 (i.e., about 26.5% of the ... This rate is similar to other single stranded DNA viruses. Porcine parvovirus is ubiquitous among swine throughout the world. ... Many cells in such cultures are in the S phase (i.e., the DNA synthesis phase) of their cell cycle, wherein the DNA polymerases ... and extracted virion DNA are 1.38-1.395, 1.30-1.315, and 1.724 respectively. Viral infectivity, hemagglutinating activity, and ...

Paborsky, L. R.; McCurdy, S. N.; Griffin, L. C.; Toole, J. J.; Leung, L. L. (1993-10-05). "The single-stranded DNA aptamer- ... BC-007 is a single-stranded DNA oligonucleotide aptamer with the nucleotide sequence 5'-GGT TGG TGT GGT TGG-3'. The proposed ... Bock, Louis C.; Griffin, Linda C.; Latham, John A.; Vermaas, Eric H.; Toole, John J. (1992). "Selection of single-stranded DNA ... BC-007 is an oligonucleotide aptamer, a synthetic DNA compound designed to bind other chemicals. BC-007 is in early-stage ...

Other chemicals, known as DNA intercalators, fit into the gap between adjacent bases on a single strand and induce frameshift ... Putnam CD (September 2021). "Strand discrimination in DNA mismatch repair". DNA Repair. 105: 103161. doi:10.1016/j.dnarep. ... 1,000,000,000 bp For single-stranded DNA/RNA, units of nucleotides are used-abbreviated nt (or knt, Mnt, Gnt)-as they are not ... The following DNA sequences illustrate pair double-stranded patterns. By convention, the top strand is written from the 5′-end ...

Formation of the RNA-DNA hybrid would protect the invading single-stranded DNA from degradation. After the transient RNA-DNA ... Thus DNA synthesis fills in gaps left over from annealing, and extends both ends of the still present single stranded DNA break ... ended single DNA strand that in the next step invades a homologous DNA duplex. RNA polymerase III is reported to catalyze ... Kaur H, De Muyt A, Lichten M (February 2015). "Top3-Rmi1 DNA single-strand decatenase is integral to the formation and ...

This property is what gives DNA its semi-conservative nature where one strand of new DNA is from an original parent strand. ... Genes are arranged linearly along long chains of DNA base-pair sequences. In bacteria, each cell usually contains a single ... DNA normally exists as a double-stranded molecule, coiled into the shape of a double helix. Each nucleotide in DNA ... Restriction enzymes are commonly used enzymes that cut DNA at specific sequences, producing predictable fragments of DNA. DNA ...

... a group at the University of Washington has proposed using double stranded DNA (dsDNA) between each single stranded molecule to ... "Recognizing a single base in an individual DNA strand: a step toward DNA sequencing in nanopores". Angewandte Chemie. 44 (9): ... In 1989 he sketched out a plan to drive a single-strand of DNA through a protein nanopore embedded into a thin membrane as part ... Fologea D, Gershow M, Ledden B, McNabb DS, Golovchenko JA, Li J (October 2005). "Detecting single stranded DNA with a solid ...

... can be used to form single stranded DNA from double stranded DNA, which is then used for DNA sequencing in the ... Wooddell, C I; Burgess, R R (1996). "Use of Asymmetric PCR to Generate Long Primers and Single-stranded DNA for Incorporating ... Single stranded DNA is also important for aptamer generation. Citartan, Marimuthu; et al. (December 2011). "Asymmetric PCR for ... Asymmetric PCR is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other. The ...

Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2A, are ubiquitous and essential for a variety of DNA metabolic ... "Single-stranded DNA-binding protein hSSB1 is critical for genomic stability". Nature. 453 (7195): 677-81. Bibcode:2008Natur.453 ... "HSSB1 and hSSB2 form similar multiprotein complexes that participate in DNA damage response". The Journal of Biological ...

DNA becomes susceptible to deamination events when it is single stranded. When replication forks form, the strand not being ... Segments with labels on the inside reside on the B strand of DNA, segments with labels on the outside are on the A strand. ... Chloroplast DNA has long been thought to have a circular structure, but some evidence suggests that chloroplast DNA more ... Chloroplast DNA (cpDNA) is the DNA located in chloroplasts, which are photosynthetic organelles located within the cells of ...