PCR analysis and sequencing of the treated organ tissues revealed large amounts of CaCV DNA and canine parvovirus-2 DNA and ... Rolling circle replication is a method for replicating genomes that is seen most commonly in circular plasmids and genomes of ... These regions contain distinctive sequences for stemloop structures, in which the single stranded DNA pairs up and a ring is ... The genome of CaCV is made up of a single circular strand of DNA 2,063 nucleotides in length. DNA in general is made up of four ...
2000). "DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae" (PDF). Nature. 406 (6795): 477-83. Bibcode: ... And, unlike plasmids, chromosomes are not self-transmissible. However, the second chromosome may have once been a megaplasmid ... V. cholerae (and Vibrionaceae in general) has two circular chromosomes, together totalling 4 million base pairs of DNA sequence ... V. cholerae has two circular DNA. One DNA produces the cholera toxin (CT), a protein that causes profuse, watery diarrhea ( ...
Finally this method is independent of the length of DNA template sequence, and requires a small amount of parental DNA.[page ... The DNA cassette and the target plasmid are both treated with endonucleases to cleave these restriction sites and create sticky ... and BALIBASE in order to cross reference target protein sequences with known sequences. Multiple sequence alignment techniques ... These selected phages are then subjected to DNA sequencing.[page needed] Cell surface display systems can also be utilized to ...
The nucleotide sequences of interest are preserved as inserts to a plasmid or the genome of a bacteriophage that has been used ... To extract DNA for genomic DNA (also known as gDNA) libraries, a DNA mini-prep may be useful. cDNA libraries require care to ... but in general each DNA fragment is uniquely inserted into a cloning vector and the pool of recombinant DNA molecules is then ... the tissue source of the genomic DNA is unimportant because each cell of the body contains virtually identical DNA (with some ...
Relic DNA can be up to 40% of the sequenced DNA in soil, and was up to 33% of the total bacterial DNA on average in a broader ... As phages, viruses, plasmids, prions, viroids, and free DNA are usually not considered as living microorganisms, they do not ... Heather, James M.; Chain, Benjamin (2016). "The sequence of sequencers: The history of sequencing DNA" (PDF). Genomics. 107 (1 ... Discovery of DNA, the development of sequencing technologies, PCR, and cloning techniques enabled the investigation of ...
A plasmid named pCG113 containing kanamycin resistance genes and the IS1096 insertion sequence was engineered to contain ... The two ends are brought together, and through oligomerization of DNA, the sequence is cut out of the chromosome. After ... The IS50R sequence codes for two proteins, Tnp and Inh. These two proteins are identical in sequence, save for the fact that ... The binding interactions between transposase and these sequences is very complicated, and is affected by DNA methylation and ...
Staden R (June 1979). "A strategy of DNA sequencing employing computer programs". Nucleic Acids Research. 6 (7): 2601-10. doi: ... Bristol (strain N2)". Plasmid. 22 (1): 10-21. doi:10.1016/0147-619x(89)90031-0. PMID 2550981. "Caenorhabditis". Merriam-Webster ... The C. elegans Sequencing Consortium (December 1998). "Genome sequence of the nematode C. elegans: a platform for investigating ... These findings indicate that DNA repair capability underlies longevity. The capacity to repair DNA damage by the process of ...
The Haseltine lab was among the first to apply the new techniques of DNA sequencing to the understanding of DNA damage and its ... Plasmid. 11 (3): 253-259. doi:10.1016/0147-619x(84)90032-5. PMID 6087393. Royer-Pokora, B; Haseltine WA (1984). "Isolation of ... Grunberg, SM; Haseltine WA (1980). "Use of an Indicator Sequence of Human DNA to Study DNA Damage by Methylbis(2-chloreothyl) ... "Cleavage of Pyrimidine Dimers in Specific DNA Sequences by a Pyrimidine Dimer DNA-Glycosylase of M.luteus". Nature. 285 (5767 ...
Zhurkin VB, Tolstorukov MY, Xu F, Colasanti AV, Olson WK (2005). "Sequence-Dependent Variability of B-DNA". DNA Conformation ... the same team from the Scripps Research Institute reported that they synthesized a stretch of circular DNA known as a plasmid ... A base-paired DNA sequence: ATCGATTGAGCTCTAGCG TAGCTAACTCGAGATCGC The corresponding RNA sequence, in which uracil is ... DNA sequences have been described which use newly created nucleobases to form a third base pair, in addition to the two base ...
... for work she did with Frederick Sanger at the MRC Laboratory of Molecular Biology developing methods to sequence DNA using RNA ... Blackburn and colleague Jack Szostak showed the unstable replicating plasmids of yeast were protected from degradation, proving ... Since DNA polymerase only synthesizes DNA in the leading strand direction, the shortening of the telomere results. Through ... Telomerase works by adding base pairs to the overhang of DNA on the 3' end, extending the strand until DNA polymerase and an ...
The tree was constructed by comparing the peptide sequences of ten different types of proteins. The proteins used were the DNA ... There are neither any plasmids nor any other mobile genetic elements. The genome contains a total of 263 genes: 227 protein ... K. muelleri" has a minimal set of genes assigned for DNA housekeeping purposes. The only genes it has for DNA repair are the ... K. muelleri", strain GWSS genome was completely sequenced at McDonnell Genome Institute using Illumina dye sequencing. The ...
... an important role in regulating gene expression since they mark the end of a gene transcript and determine which DNA sequences ... In this process, the target gene is inserted into a plasmid and is regulated by the T7 promoter; T7 RNA polymerase is used to ... unwinds the helical structure of the DNA, and uses the single-stranded DNA as a template to synthesize RNA. Once RNA polymerase ... termination occurs when a specific sequence on the growing RNA strand elicits detachment of RNA polymerase from the RNA-DNA ...
... and DNA recovery by ethanol precipitation were used. The 16S ribosomal RNA gene sequence of H. larsenii was studied using ARB ... The genome consists of 2.79 Mega-bases on a circular chromosome with four circular plasmids. The genome includes 4,246 genes of ... In 2014, the complete genome of H. larsenii was sequenced using Illumina dye sequencing HiSeq 2000 by Iain Anderson as part of ... The GC-content of the genome is 61%. In a 2008 study by Castillo, et al., chromosomal DNA was isolated using the Marmur methods ...
First, restriction enzymes are used to cleave near the target sequence on DNA contained in a suitable vector. This step removes ... can be performed in the same plasmid. Worrall, Andrew (1994). "Site-Directed Mutagenesis by the Cassette Method". Methods in ... To use this method, the sequence of the target sequence and nearby restriction sites must be known. Since restriction enzymes ... Finally, the resultant construct is sequenced to check that the target sequence contains the intended mutation. The use of ...
Arsenite-Antimonite efflux Silver S, Misra TK, Laddaga RA (1989). "DNA sequence analysis of bacterial toxic heavy metal ... preliminary x-ray analysis of the catalytic subunit of the ATP-dependent arsenite pump encoded by the Escherichia coli plasmid ...
... fuses with a host plasmid (the target plasmid). In the process, the transposon and a short section of host DNA are replicated. ... Akopian A, He J, Boocock MR, Stark WM (July 2003). "Chimeric recombinases with designed DNA sequence recognition". Proc. Natl. ... one on each strand of the donor plasmid and one on each strand of the target plasmid. The donor and target plasmids are ligated ... Reed RR, Grindley ND (September 1981). "Transposon-mediated site-specific recombination in vitro: DNA cleavage and protein-DNA ...
Sequence the DNA: After final step of selection and electrophoresis, clone the DNA into a cloning vector and sequence it. ... The recombinant plasmids were transformed into competent Escherichia coli strain BL21 and Quox1 fusion proteins were isolated ... After 5 set of the same procedure, the purified DNA was cloned into pMD 18T and sequenced. Finally the sequence CAATC was ... Isolate the DNA bound protein by EMSA: the DNA bound protein, migrated in acrylamide gel, is isolated by autoradiography as per ...
Chang C, Meyerowitz EM (March 1986). "Molecular cloning and DNA sequence of the Arabidopsis thaliana alcohol dehydrogenase gene ... involves simply dipping flowers into a solution containing Agrobacterium carrying a plasmid of interest and a detergent. This ... The DNA of plants is vulnerable to ultraviolet light, and DNA repair mechanisms have evolved to avoid or repair genome damage ... Arabidopsis Biological Resource Center (seed and DNA stocks) Nottingham Arabidopsis Stock Centre (seed and DNA stocks) Artade ...
The DNA sequences bound by mecI and blaI are identical; therefore, blaI can also bind the mecA operator to repress ... Carriage of large plasmids, such as SCCmecI-III, is costly to the bacteria, resulting in a compensatory decrease in virulence ... in 2001, and can be explained by the fitness differences associated with carriage of a large or small SCCmec plasmid. ... Examples of such elements include plasmids, transposable genetic elements, and genomic islands, which can be transferred ...
Target proteins or DNA sequences are immobilized to the wells of a microtiter plate. Many genetic sequences are expressed in a ... Loss of phage infectivity can be avoided by using a phagemid plasmid and a helper phage so that the resultant phage contains ... from which the phagemids can be collected and the relevant DNA sequence excised and sequenced to identify the relevant, ... The proteins that the phages are displaying can then be screened against other proteins, peptides or DNA sequences, in order to ...
A conjugative plasmid capable of chromosome integration is also called an episome (a segment of DNA that can exist as a plasmid ... cells do not receive the complete F factor sequence, and do not become F + due to its inability to form a sex pilus. In ... Thus F-prime plasmid is the plasmid, containing part of the chromosomal DNA which can be transferred to recipient cell, along ... F-prime cell contains F-plasmid that integrates with the chromosomal DNA and carries part of the chromosomal DNA along with it ...
When this strain was sequenced, it was found to have a 4.6 Mb circular chromosome and three plasmids. A comparative genomic and ... Two strains of P. stutzeri can be classified in a single genomovar if DNA-DNA similarity is at least 70% similar. Seven ... bacterium into the Pseudomonas genus was confirmed by DNA-DNA hybridization and similarity comparisons of the rRNA sequences. ... Sequencing the genome of the P. stutzeri strain DSM4166 revealed some genes for nitrogen fixation, along with 42 genes that ...
... derived from the 2 µ plasmid of baker's yeast Saccharomyces cerevisiae. The 34bp minimal FRT site sequence has the sequence ... Senecoff JF, Rossmeissl PJ, Cox MM (May 1988). "DNA recognition by the FLP recombinase of the yeast 2 mu plasmid. A mutational ... It does so by causing recombination between the two inverted repetitions on the 2 µ plasmid during DNA replication. This ... The nucleophilic properties of the tyrosine attack and bind to the 3'-phosphate at the point of DNA cleavage. The resulting 5'- ...
X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Research. 5 (3): ... "Ultrastructural localization of ras-related proteins using epitope-tagged plasmids". The Journal of Histochemistry and ... "Entrez Gene: RHOB ras homolog gene family, member B". Chardin P, Madaule P, Tavitian A (March 1988). "Coding sequence of human ... Chardin P, Madaule P, Tavitian A (March 1988). "Coding sequence of human rho cDNAs clone 6 and clone 9". Nucleic Acids Research ...
DNA sequences in or around genes that act as target sites for DNA-binding proteins. By binding to cis-regulatory sequences and ... Transformation of a bacterial host with two different plasmids is required. One is designed to express a DNA-binding protein-of ... positive clones are sequenced and examined with motif-finding tools in order to resolve the favoured DNA target sequence. ... The bacterial one-hybrid (B1H) system is a method for identifying the sequence-specific target site of a DNA-binding domain. In ...
... variation in DNA sequence within the recognition sites for enzymes, short distance between regions of unusual DNA sequences and ... When examining the preferential double strand cleavage of DNA, the restriction endonuclease PstI bind to pSM1 plasmid DNA. PstI ... PstI cleaves DNA at the recognition sequence 5′-CTGCA/G-3′ generating fragments with 3′-cohesive termini. This cleavage yields ... is a useful enzyme for DNA cloning as it provides a selective system for generating hybrid DNA molecules. These hybrid DNA ...
Sometimes this limitation is circumvented simply by cloning the isolated genomic DNA into a plasmid vector and then using ... Alternatively, when one wants to find where the protein binds on a genome-wide scale, ChIP-sequencing is used and has recently ... The purified protein-DNA complexes are then heated to reverse the formaldehyde cross-linking of the protein and DNA complexes, ... By using an antibody that is specific to a putative DNA binding protein, one can immunoprecipitate the protein-DNA complex out ...
... yielding different proteins from the same DNA sequence". He has been selected to receive the 2015 Othmer Gold Medal. Sharp's ... where he studied plasmids. Later, he studied gene expression in human cells at the Cold Spring Harbor Laboratory as a senior ... and has discovered a class of miRNAs that are produced from sequences adjacent to transcription start sites. His laboratory ...
Thus the sequence of scAAV is likely unwound by a virally encoded DNA helicase before being packaged into viral protein capsid ... In lab use, this obstacle is overcome by addition of the helper plasmids, which exogenously expresses replication genes which ... These viruses are nonenveloped, single-strand DNA (ssDNA) viruses. Within Parvoviridae, scAAV further belongs to the ... "higher in vivo DNA stability and more effective circularization." scAAV is an attractive vector for use in gene therapy for ...
"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans". DNA Research. 11 (3): 179-197. doi: ... Like Y. pseudotuberculosis and Y. enterocolitica, Y. pestis is host to the plasmid pCD1. It also hosts two other plasmids, ... Plasmids of Y. pestis were detected in archaeological samples of the teeth of seven Bronze Age individuals, in the Afanasievo ... DNA evidence published in 2015 indicates Y. pestis infected humans 5,000 years ago in Bronze Age Eurasia, but genetic changes ...
The resulting double stranded DNA is then incorporated into E.coli plasmids. Each bacterium now contains one unique cDNA and is ... The resultant part of the sequence was termed the expressed sequence tag (EST) and is always at the end of the sequence close ... The sequencing of cDNA will produce the entire mRNA transcript that generated it. Practically, only part of the sequence is ... The library can then sequenced by high-throughput sequencing techniques. This can characterise both the different genes ...
This sequence is used to package the genome into the virion. As opposed to lentiviruses, adenoviral DNA does not integrate into ... One or more plasmids, generally referred to as packaging plasmids, encode the virion proteins, such as the capsid and the ... Because these diseases result from mutations in the DNA sequence for specific genes, gene therapy trials have used viruses to ... While this limits the introduction of many genomic sequences, most cDNA sequences can still be accommodated. The primary ...
... recognising a particular short DNA sequence, or restriction site, and cutting both DNA strands at specific points within this ... for example the relatively small DNA molecules of viruses and plasmids can be identified simply by their restriction fragment ... Many cuts are made by one restriction enzyme because of the chance repetition of these sequences in a long DNA molecule, ... A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction ...