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*  Thermal cycler
The earliest thermal cyclers were designed for use with the Klenow fragment of DNA polymerase I. Since this enzyme is destroyed ... including restriction enzyme digestion or rapid diagnostics. The device has a thermal block with holes where tubes holding the ... The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to ... Weier, HU; Gray, JW (Jul-Aug 1988). "A programmable system to perform the polymerase chain reaction". DNA (Mary Ann Liebert, ...
*  Amplified fragment length polymorphism
... cellular DNA with one or more restriction enzymes and ligation of restriction half-site specific adaptors to all restriction ... AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction ... Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Office, publication 0 ... the restriction site sequence and a few nucleotides inside the restriction site fragments (as described in detail below). The ...
*  Terminal restriction fragment length polymorphism
... using one or more restriction enzymes and detecting the size of each of the individual resulting terminal fragments using a DNA ... The mixture of amplicons is then subjected to a restriction reaction, normally using a four-cutter restriction enzyme. ... Attempts to minimize (but not overcome) this problem are often done by applying several restriction enzymes and/ or labeling ... give a single peak on the electropherogram due to the presence of a restriction site for the particular restriction enzyme used ...
*  Inverse polymerase chain reaction
... cutting restriction enzyme. Under low DNA concentrations, self-ligation is induced to give a circular DNA product. PCR is ... PCR does not require linear products and the use of another restriction enzyme to cut the known sequence could also cut within ... Then, like other polymerase chain reaction processes, the DNA is amplified by the temperature-sensitive DNA polymerase: A ... genomic DNA. The amplified product can then be sequenced and compared with DNA databases to locate the sequence which has been ...
*  Restriction site
Some restriction enzymes cut DNA at a restriction site in a manner which leaves no overhang, called a blunt end. Blunt ends are ... because restriction enzymes usually bind as homodimers), and a particular restriction enzyme may cut the sequence between two ... Restriction sites, or restriction recognition sites, are locations on a DNA molecule containing specific (4-8 base pairs in ... For example, the common restriction enzyme EcoRI recognizes the palindromic sequence GAATTC and cuts between the G and the A on ...
*  Molecular cloning
... see DNA end). Typically, this is done by cleaving the vector DNA and foreign DNA with the same restriction enzyme, for example ... enzymes that could cleave DNA molecules only when specific DNA sequences were encountered. They showed that restriction enzymes ... Using a second enzyme, DNA ligase, fragments generated by restriction enzymes could be joined in new combinations, termed ... The purified DNA is then treated with a restriction enzyme to generate fragments with ends capable of being linked to those of ...
*  Escherichia coli in molecular biology
The work of Stanley Norman Cohen and Herbert Boyer in E. coli, using plasmids and restriction enzymes to create recombinant DNA ... E. coli genotypes - OpenWetWare Meselson, M; Yuan, R (1968). "DNA restriction enzyme from E. Coli". Nature. 217 (5134): 1110-4 ... One of the first useful applications of recombinant DNA technology was the manipulation of E. coli to produce human insulin. ... Modified E. coli have been used in vaccine development, bioremediation, and production of immobilised enzymes. E. coli have ...
*  Daniel Nathans
Nobel prizes for the studies on DNA restriction enzymes]". Postepy Biochem. 25 (2): 251-3. PMID 388391. Desiderio, S; Boyer S ( ... He shared the 1978 Nobel Prize in Physiology or Medicine for the discovery of restriction enzymes. Nathans was born in ... Restriction enzymes and the boon to modern molecular biology". Proc. Natl. Acad. Sci. U.S.A. 102 (17): 5909. doi:10.1073/pnas. ...
*  Werner Arber
Nobel prizes for the studies on DNA restriction enzymes". Postepy biochemii. 25 (2): 251-3. ISSN 0032-5422. PMID 388391. Berg, ... Their work would lead to the development of recombinant DNA technology. Arber studied chemistry and physics at the Swiss ... Werner Arber shared the 1978 Nobel Prize in Physiology or Medicine for the discovery of restriction endonucleases. ...
*  Timeline of biology and organic chemistry
1970 - Hamilton Smith and Daniel Nathans discovered DNA restriction enzymes. 1970 - Howard Temin and David Baltimore ... 1955 - Arthur Kornberg discovered DNA polymerase enzymes. 1958 - John Gurdon used nuclear transplantation to clone an African ... 1952 - Rosalind Franklin concluded that DNA is a double helix with a diameter of 2 nm and the sugar-phosphate backbones on the ... 1948 - Erwin Chargaff showed that in DNA the number of guanine units equals the number of cytosine units and the number of ...
*  Biological small-angle scattering
1 Jan 2012). "Structure and operation of the DNA-translocating type I DNA restriction enzymes". Genes & Development. 26: 92-104 ... An example in which SAXS, SANS and EM data has been used to build an atomic model of a large multi-subunit enzyme has recently ... Ho, D. L.; Byrnes, W. M.; Ma, W.-p.; Shi, Y.; Callaway, D. J. E.; Bu, Z. (2004). "Structure-specific DNA-induced Conformational ... "Quaternary structures of tumor suppressor p53 and a specific p53-DNA complex". Proc Natl Acad Sci USA. 104 (30): 12324-12329. ...
*  Small-angle neutron scattering
1 Jan 2012). "Structure and operation of the DNA-translocating type I DNA restriction enzymes". Genes & Development. 26 (4): 92 ... An example in which SAXS, SANS and EM data has been used to build an atomic model of a large multi-subunit enzyme has recently ... This can be based on inherent scattering differences, e.g. DNA vs. protein, or arise from differentially labeled components, e. ...
*  Rhesus macaque
... as revealed by mitochondrial DNA restriction enzyme analysis". International Journal of Primatology. 14 (4): 587. doi:10.1007/ ... DNA from different branches of the primate tree will allow us "to trace back the evolutionary changes that occurred at various ... The DNA microarray showed the macaque genomic response to human influenza on a cellular level in each tissue. Both viruses ... The DNA response showed a transition from innate to adaptive immune response over seven days. The full sequence and annotation ...
*  Pygmy slow loris
... and mitochondrial DNA restriction enzyme analysis. The phylogenetic relationships within the genus Nycticebus have been studied ... an approach using mitochondrial DNA restriction enzyme analysis". International Journal of Primatology. 14 (1): 167-175. doi: ... inferred from ribosomal DNA restriction maps". Zoological Research. 17 (1): 89-93. CNKI:SUN:DWXY.0.1996-01-015. (Subscription ... using DNA sequences derived from the mitochondrial DNA markers D-loop and cytochrome b from 22 slow loris individuals. In this ...
*  HpaII
... is a restriction enzyme obtained from the microorganism called Haemophilus parainfluenzae. It is a DNA restriction enzyme, ... the enzyme will cut 300 times slower than unmethylated DNA and 50 times slower if the DNA is hemi-methylated. This feature is ... therefore it has the ability to cut the DNA from certain region as demonstrated below. It has the ability to produce cohesive ...
*  Endodeoxyribonucleases
Examples include: DNA restriction enzymes micrococcal nuclease Endodeoxyribonucleases at the US National Library of Medicine ... Endodeoxyribonucleases are enzymes which are both deoxyribonucleases and endonucleases. They are classified with EC numbers 3.1 ...
*  Rebase (database)
Restriction enzymes DNA methyltransferases Roberts, Richard J; Vincze Tamas; Posfai Janos; Macelis Dana (Jan 2010). "REBASE--a ... In molecular biology, REBASE is a database of information about restriction enzymes and DNA methyltransferases. REBASE contains ... database for DNA restriction and modification: enzymes, genes and genomes". Nucleic Acids Res. England. 38 (Database issue): ... REBASE is one of the longest running biological databases having its roots in a collection of restriction enzymes maintained by ...
*  Argonaute
... along with guide DNA to edit DNA as artificial restriction enzymes. However, evidence for Argonaute proteins as DNA-guided DNA ... Revolutionizing Biotechnology with Artificial Restriction Enzymes. (reporting on Programmable DNA-Guided Artificial Restriction ... Because it has been widely known that many viruses have RNA rather than DNA as their genetic material and go through at least ... In many eukaryotes, including animals, the RNA interference pathway is found, and it is initiated by the enzyme Dicer. Dicer ...
*  Raccoonpox virus
Differences between terminal region patterns of DNA restriction enzyme digests of different RCN isolates suggest that varied ... RCN is a double-stranded DNA virus. It is an orthopoxvirus in the family Poxviridae and subfamily Chordopoxvirinae. DNA ... Raccoonpox virus (RCN) is a double-stranded DNA virus and a member of the orthopoxviruses in the family Poxviridae and ... RCN tandem repeat sequences found on the terminal Sal1 restriction fragment were found to be distinct from varicella and cowpox ...
*  List of restriction enzyme cutting sites: Bst-Bv
Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds. Cut: Cutting site and DNA ... "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7 ... This article contains a list of the most studied restriction enzymes whose names start with Bst to Bv inclusive. It contains ... Further reading: see the section "Nomenclature" in the article "Restriction enzyme".) PDB code: Code used to identify the ...
*  Richard J. Roberts
subscription required) Roberts, Richard John (2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing ... The realisation that individual genes could exist as separate, disconnected segments within longer strands of DNA first arose ... a co-discoverer of the structure of DNA and a fellow Nobel laureate. In 1992, he moved to New England Biolabs. The following ... "Hhal methyltransferase flips its target base out of the DNA helix". Cell. 76 (2): 357-369. doi:10.1016/0092-8674(94)90342-5. ...
*  Phasevarion
"A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7 ... and serve to protect host DNA from the action of the associated restriction enzyme. However, in many bacterial pathogens, mod ... and the associated restriction enzyme encoding gene (res) is inactive. In these organisms the DNA methyltransferase phase ... Methylation of unique DNA sequences results in different Mod enzymes that regulate the expression of different sets of genes; i ...
*  Ribotyping
It involves the digestion of bacterial genomic DNA with specific restriction enzymes. Each restriction enzyme cuts DNA at a ... "Restriction Enzymes , ASU - Ask A Biologist". askabiologist.asu.edu. Retrieved 2016-03-13. "ribotype". courses.cit.cornell.edu ... Conceptually, ribotyping is similar to probing restriction fragments of chromosomal DNA with cloned probes (randomly cloned ... The pattern is then digitized and used to identify the origin of the DNA by a comparison with reference organisms in a computer ...
*  R.EcoRII
2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids ... EcoRI, another nuclease enzyme from Escherichia coli. EcoRV, another nuclease enzyme from Escherichia coli. B3 DNA binding ... Restriction endonuclease (REase) EcoRII (pronounced "eco R two") is an enzyme of restriction modification system (RM) naturally ... "Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific ...
*  STR analysis
... since STR analysis does not cut the DNA with restriction enzymes. Instead, probes are attached to desired regions on the DNA, ... However, DNA database searches showed much more frequent than expected false DNA profile matches. Moreover, since there are ... National Commission on the Future of DNA Evidence (July 2002). "Using DNA to Solve Cold Cases" (pdf). U.S. Department of ... The system of DNA profiling used today is based on PCR and uses simple sequences or short tandem repeats (STR). This method ...
*  Phage display
... and in searching for protein-DNA interactions using specially-constructed DNA libraries with randomised segments. Competing ... The size restriction seems to have less to do with structural impediment caused by the added section and more to do with the ... It is used for finding new ligands (enzyme inhibitors, receptor agonists and antagonists) to target proteins. The technique is ... In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or ...
*  Ice-minus bacteria
... syringae's DNA with restriction enzymes. Insert the individual DNA pieces into a plasmid. Pieces will insert randomly, allowing ... The plasmid will be taken in by the bacteria, rendering it part of the organism's DNA. Identify the ice-gene from the numerous ... He was later successful at developing the ice-minus strain of P. syringae through recombinant DNA technology as well. In 1983, ... Both strains of P. syringae occur naturally, but recombinant DNA technology has allowed for the synthetic removal or alteration ...