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*  Genetic recombination
Recombination can be artificially induced in laboratory (in vitro) settings, producing recombinant DNA for purposes including ... It is a process by which a DNA sequence is copied from one DNA helix (which remains unchanged) to another DNA helix, whose ... creating what is called recombinant DNA. A prime example of such a use of genetic recombination is gene targeting, which can be ... or by the breaking and rejoining of DNA strands, which forms new molecules of DNA (see DHJ pathway in Figure). Recombination ...
*  BacMam
Using complementary DNA (cDNA) microarray technology to examine in vitro and in vivo global cellular gene expression profiles ... US patent #5,871,986 Kost, T; Condreay, JP (2002). "Recombinant baculoviruses as mammalian cell gene-delivery vectors". Trends ... "BacMam Recombinant Baculoviruses in G Protein?Coupled Receptor Drug Discovery". Receptors and Channels. 10 (3-4): 99-107. doi: ... "BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies". Protein Expr ...
*  Homologous recombination
Many methods for introducing DNA sequences into organisms to create recombinant DNA and genetically modified organisms use the ... New DNA synthesis fills in any gaps, and ligation restores the DNA duplex as two continuous strands. The DNA sequence between ... Resolution of the Holliday junction by some combination of RuvABC or RecG can produce two recombinant DNA molecules with ... Foreign, bacterial DNA is sometimes misincorporated in the capsid head of bacteriophage virus particles as DNA is packaged into ...
*  Baculoviridae
Altmann, Friedrich; Staudacher, E; Wilson, IB; März, L (1999). "Insect cells as hosts for the expression of recombinant ... that encloses the DNA with p6.9 protein. BV acquires its envelope from the cell membrane and requires a glycoprotein, gp64, to ... Kost, T; Condreay, JP (1999). "Recombinant baculoviruses as expression vectors for insect and mammalian cells". Current Opinion ... Baculovirus expression in insect cells represents a robust method for producing recombinant glycoproteins or membrane proteins ...
*  Molecular cloning
Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is ... Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into host organism, (6) ... and the recombinant DNA molecule, are commonly referred to as "clones". Strictly speaking, recombinant DNA refers to DNA ... termed recombinant DNA. By recombining DNA segments of interest with vector DNA, such as bacteriophage or plasmids, which ...
*  Recombinant DNA
For example, plant DNA may be joined to bacterial DNA, or human DNA may be joined with fungal DNA. In addition, DNA sequences ... Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into the host organism, ( ... and incorporated into recombinant molecules. Using recombinant DNA technology and synthetic DNA, literally any DNA sequence may ... Animation illustrating construction of recombinant DNA and foreign protein production by recombinant bacteria Recombinant DNA ...
*  Asilomar Conference on Recombinant DNA
The Basics of Recombinant DNA Provides an introduction to the science behind recombinant DNA. china The Recombinant DNA Debate ... "Recombinant DNA Technology and Its Social Transformation", p. 303 Wright, "Recombinant DNA Technology and Its Social ... scientists should halt experiments involving recombinant DNA technology. The Asilomar Conference on Recombinant DNA took place ... One of these capacities was recombinant DNA technology. This technology entails the joining of DNA from different species and ...
*  Norman Wengert
Science Policy Implications of DNA Recombinant Molecule Research. 95th Cong., 1st sess. Washington, D.C.: U.S. Government ... "Public Participation in Scientific and Technical Decision Making." In: Science Policy Implications of DNA Recombinant Molecule ...
*  Marker gene
Recombinant DNA. ... only one in a several million to billion cells may take up DNA ... a marker gene is a gene used to determine if a nucleic acid sequence has been successfully inserted into an organism's DNA. In ... scientists use a selective agent to kill all cells that do not contain the foreign DNA, leaving only the desired ones. ...
*  X-gal
Sandhu, Sardul Singh (2010). Recombinant DNA Technology. I K International Publishing House. p. 116. ISBN 978-9380578446. "5- ... Joung J, Ramm E, Pabo C (2000). "A bacterial two-hybrid selection system for studying protein-DNA and protein-protein ...
*  Anwar Nasim
Dillon, J.R., NASIM, A., Nestmann, E.R. (1985). Recombinant DNA Methodology. John Wiley & Sons, New York . Translated into ... Repairable Lesions in Microorganisms[permanent dead link] Recombinant DNA Methodology[permanent dead link] Molecular Biology of ... In 1978 he was chairman of the organizing committee for the International Symposium on DNA repair, Mutation and Human III ...
*  Gonadotropin preparations
Initial results demonstrates similar pregnancy rates as daily recombinant FSH injections. Prepared from recombinant DNA. ... as well as recombinant preparations. The recombinant preparations are more pure and more easily administered, but they are more ... There are also recombinant variants. hMG (human Menopausal Gonadotrophins), FSH and LH prepared from human urine collected from ... "Corifollitropin alfa demonstrates similar pregnancy rates as compared to daily recombinant FSH treatment in a controlled ...
*  Transgenesis
"Transgenic organisms". Gene Manipulation & Recombinant DNA. University of Westminster. Retrieved June 28, 2014. Kyndt, Tina; ... This allows the foreign DNA to hybridise with the plasmid DNA and be sealed by DNA ligase enzyme, creating a genetic code not ... The plasmid DNA is cut using restriction enzymes, while the DNA to be copied is also cut with the same restriction enzyme, ... DNA microinjection does not have a high success rate (roughly 2% of all injected subjects), even if the new DNA is incorporated ...
*  Maxine Singer
In the wake of the 1973 report of the first use of recombinant DNA techniques to introduce genes from one species into another ... Singer, M.; Berg, P. (1976-07-16). "Recombinant DNA: NIH Guidelines". Science. 193 (4249): 186-188. doi:10.1126/science. ... she raised concerns over the potential health effects and risks in the relatively new field of recombinant DNA technology. She ... and she helped to organize the 1975 Asilomar Conference on Recombinant DNA that resulted in guidelines for dealing with the ...
*  Heterologous expression
Recombinant DNA Binder, Marc D.; Hirokawa, Nobutaka; Windhorst, Uwe, eds. (2009). Encyclopedia of Neuroscience. Springer Berlin ... not naturally have this gene or gene fragment.Insertion of the gene in the heterologous host is performed by recombinant DNA ... After being inserted in the host, the gene may be integrated into the host DNA causing permanent expression, or not integrated ...
*  Neurogenetics
The use of recombinant DNA is an example of a reverse genetics, where researchers create a mutant genotype and analyze the ... Recombinant DNA is an important method of research in many fields, including neurogenetics. It is used to make alterations to ... By the late 1980s new advances in genetics such as recombinant DNA technology and reverse genetics allowed for the broader use ... Kuure-Kinsey, Matthew; McCooey, Beth (2006). The Basics of Recombinant DNA. Ambrose, Victor (2011). Reverse Genetics. Pfeiffer ...
*  Ice-minus bacteria
Insert the individual DNA pieces into a plasmid. Pieces will insert randomly, allowing for different variations of recombinant ... He was later successful at developing the ice-minus strain of P. syringae through recombinant DNA technology as well. In 1983, ... Both strains of P. syringae occur naturally, but recombinant DNA technology has allowed for the synthetic removal or alteration ... 18 (1). CS1 maint: Uses authors parameter (link) Parrott, Carolyn C. (1993). "Recombinant DNA to Protect Crops". Archived from ...
*  Quantitative genetics
Watson, James D.; Gilman, Michael; Witkowski, Jan; Zoller, Mark (1998). Recombinant DNA (Second (7th printing) ed.). New York: ... A QTL is a region in the DNA genome that effects, or is associated with, quantitative phenotypic traits. ...
*  Tissue plasminogen activator
... recombinant tPA is recommended. tPA was first produced by recombinant DNA techniques at Genentech in 1982.[citation needed] ... "Pharmaceutical Products of Recombinant DNA Technology". Biology Discussion. 2015-09-21. Retrieved 2017-12-10. Pennica, Diane; ... tPA can be manufactured using recombinant biotechnology techniques and is then referred to as recombinant tissue plasminogen ... Wardlaw JM, Murray V, Berge E, del Zoppo G, Sandercock P, Lindley RL, Cohen G (June 2012). "Recombinant tissue plasminogen ...
*  Biomolecular engineering
Recombinant DNA is used for a wide range of applications. The traditional method for creating recombinant DNA typically ... Recombinant DNA are DNA biomolecules that contain genetic sequences that are not native to the organism's genome. Using ... Ligases then joins the sticky ends to the corresponding sticky ends of the foreign DNA fragments creating a recombinant DNA ... Gam functions to protect the DNA insert from being destroyed by native nucleases within the cell. Recombinant DNA can be ...
*  BioDrugs
... recombinant DNA and gene regulation technology; gene therapy; nucleic acid therapeutics and diagnostics; stem cell therapies; ...
*  Economic importance of bacteria
It is also called recombinant DNA technology. In genetic engineering, pieces of DNA (genes) are introduced into a host by a ... The foreign DNA becomes a permanent feature of the host, and is replicated and passed on to daughter cells along with the rest ... of its DNA. Bacterial cells are transformed and used in production of commercially important products. The examples are ...
*  Arthur Riggs (geneticist)
In the 1980s, Riggs became convinced that the type of splicing approach used with recombinant DNA also could be used to create ... Riggs and Itakura collaborated with Herbert Boyer at Genentech, and used recombinant DNA technology to become the first to ... doi:10.1016/0022-2836(68)90261-1. Wu, Ray; Grossman, Lawrence; Moldave, Kivie (2014). Recombinant DNA Methodology Selected ... "It could have been anyone's DNA, but as a pioneer in DNA methylation epigenetics, there is something special to me about it ...
*  Science for the contemporary world
The Recombinant DNA technology and genetic engineering. Applications. - Assisted reproduction. Cloning and applications. Stem ...
*  Filgrastim
... is a human granulocyte colony stimulating factor (G-CSF) produced by recombinant DNA technology. G-CSF regulates the ... It is produced by recombinant DNA technology. The gene for human granulocyte colony-stimulating factor is inserted into the ... NEUPOGEN (filgrastim) is the name for recombinant methionyl human granulocyte colony stimulating factor (r-metHuG-CSF). ...
*  Phage display
... and in searching for protein-DNA interactions using specially-constructed DNA libraries with randomised segments. Competing ... "Recombinant human Fab fragments neutralize human type 1 immunodeficiency virus in vitro". Proc. Natl. Acad. Sci. U.S.A. 89 (19 ... In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or ... By immobilizing a relevant DNA or protein target(s) to the surface of a microtiter plate well, a phage that displays a protein ...
*  Sir William Dunn School of Pathology
Rescue of influenza A virus from recombinant DNA. Fodor E, Devenish L, Engelhardt OG, Palese P, Brownlee GG, García-Sastre A. J ... 10.1038/315683a0 Expression of active human clotting factor IX from recombinant DNA clones in mammalian cells.D. S. Anson*, D. ... providing a recombinant source of this protein for Haemophilia B patients who had proviously relied on the hazardous blood- ...