Upon binding to DNA (the "Gyrase-DNA" state), there is a competition between DNA wrapping and dissociation, where increasing ... whose single circular DNA is cut by DNA gyrase and the two ends are then twisted around each other to form supercoils. Gyrase ... DNA gyrase, or simply gyrase, is an enzyme within the class of topoisomerase and is a subclass of Type II topoisomerases that ... DNA cleavage and reunion is performed by a catalytic center located in DNA-gates build by all gyrase subunits. C-gates are ...

Novel DNA Gyrase inhibitors. At Trius Pharmaceuticals, Shaw lead the microbiology efforts in designing a screening regimen for ... "Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) and topoisomerase IV (ParE). Part I: Structure guided discovery and ... a novel class of DNA gyrase/Topoisomerase IV antibacterial agents that target both Gram-positive and Gram-negative organisms.[2 ... novel DNA gyrase/topoisomerase IV program. These efforts led to the discovery of the TriBE inhibitors, a novel class of agents ...

... including DNA gyrase) achieve this by adding negative supercoils to the DNA helix. Bare single-stranded DNA tends to fold back ... Replication machineries include primosotors are replication enzymes; DNA polymerase, DNA helicases, DNA clamps and DNA ... DNA Topoisomerases Prevent DNA Tangling During Replication Reece RJ, Maxwell A (26 September 2008). "DNA gyrase: structure and ... DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule. DNA ...

The potency of novobiocin is considerably higher than that of the fluoroquinolones that also target DNA gyrase, but at a ... X-ray crystallographic studies have found that the drug-receptor complex of Novobiocin and DNA Gyrase shows that ATP and ... Aminocoumarins are very potent inhibitors of bacterial DNA gyrase and work by targeting the GyrB subunit of the enzyme involved ... Lewis RJ, Tsai FT, Wigley DB (August 1996). "Molecular mechanisms of drug inhibition of DNA gyrase". BioEssays. 18 (8): 661-71 ...

... does not bind to DNA gyrase but does bind to the substrate DNA. A review in 2001 suggests that cytotoxicity of ... "Mechanism of inhibition of DNA gyrase by analogues of nalidixic acid: the target of the drugs is DNA". Proceedings of the ... It functions by inhibiting DNA gyrase, a type II topoisomerase, and topoisomerase IV, enzymes necessary to separate bacterial ... Drlica K, Zhao X (September 1997). "DNA gyrase, topoisomerase IV, and the 4-quinolones". Microbiology and Molecular Biology ...

Drlica K, Zhao X (September 1997). "DNA gyrase, topoisomerase IV, and the 4-quinolones". Microbiology and Molecular Biology ... Cinoxacin mode of action involves the inhibiting of DNA gyrase, a type II topoisomerase, and topoisomerase iv, which is an ... the older gyrase-inhibitors such as cinoxacin are no longer indicated. Cinoxacin is one of the original quinolone drugs, which ... enzyme necessary to separate replicated DNA, thereby inhibiting cell division. Within the most recent package insert (c. 1999) ...

"DNA gyrase, topoisomerase IV, and the 4-quinolones". Microbiol. Mol. Biol. Rev. 61 (3): 377-92. doi:10.1128/mmbr.61.3.377- ... 2 protein core L Klevan, JC Wang - Biochemistry, 1980 - ACS Publications Stabilization of Z-DNA by polyarginine near ... Wiley Online Library 31P NMR studies of the solution structure and dynamics of nucleosomes and DNA L Klevan, IM Armitage, DM ... Klevan acted as president of Applied Markets for ABI which produced and marketed reagent kits for forensic DNA, paternity ...

It functions by inhibiting DNA gyrase, a type II topoisomerase, and topoisomerase IV, enzymes necessary to separate bacterial ... Drlica K, Zhao X (September 1997). "DNA gyrase, topoisomerase IV, and the 4-quinolones". Microbiology and Molecular Biology ... It usually kills bacteria by blocking their ability to duplicate DNA. Moxifloxacin was patented in 1988 and approved for use in ... DNA, thereby inhibiting cell replication. About 52% of an oral or intravenous dose of moxifloxacin is metabolized via ...

It functions by inhibiting two bacterial type II topoisomerases, DNA gyrase and topoisomerase IV. Topoisomerase IV is an enzyme ... Drlica K, Zhao X (September 1997). "DNA gyrase, topoisomerase IV, and the 4-quinolones". Microbiology and Molecular Biology ... It works by interfering with the bacterium's DNA. Ofloxacin was patented in 1980 and approved for medical use in 1985. It is on ... necessary to separate (mostly in prokaryotes, in bacteria in particular) replicated DNA, thereby inhibiting bacterial cell ...

It functions by inhibiting DNA gyrase, a type II topoisomerase, and topoisomerase IV, enzymes necessary to separate bacterial ... 3, 1988 Drlica K, Zhao X (September 1997). "DNA gyrase, topoisomerase IV, and the 4-quinolones". Microbiology and Molecular ... Quinolone induced DNA damage was first reported in 1986 (Hussy et al.). Recent studies have demonstrated a correlation between ... There continues to be considerable debate as to whether or not this DNA damage is to be considered one of the mechanisms of ...

It functions by inhibiting DNA gyrase, a type II topoisomerase, and topoisomerase IV, which is an enzyme necessary to separate ... Drlica K, Zhao X (1 September 1997). "DNA gyrase, topoisomerase IV, and the 4-quinolones". Microbiol Mol Biol Rev. 61 (3): 377- ... replicated DNA, thereby inhibiting cell division. Tendinitis and rupture, usually of the Achilles tendon, are class-effects of ...

DNA gyrase, on the other hand, is responsible for supercoiling the DNA, so that it will fit in the newly formed cells. Both ... Drlica K, Zhao X (1 September 1997). "DNA gyrase, topoisomerase IV, and the 4-quinolones". Microbiol Mol Biol Rev. 61 (3): 377- ... Like all quinolones, it functions by inhibiting the DNA gyrase and topoisomerase IV, two bacterial type IIA topoisomerases. ... This enantiomer binds more effectively to the DNA gyrase enzyme and to topoisomerase IV than its (+)-(R)-counterpart. ...

Ashiuchi, M.; Kuwana, E; Yamamoto, T; Komatsu, K; Soda, K; Misono, H (2002). "Glutamate Racemase is an Endogenous DNA Gyrase ... Racemization and DNA gyrase inhibition are two independent activities of the enzyme". Microbiology. 154 (9): 2796-803. doi: ... Deprez, E.; Tauc, P.; Leh, H.; Mouscadet, J.-F.; Auclair, C.; Hawkins, M. E.; Brochon, J.-C. (2001). "DNA binding induces ... Streaker, Emily D.; Beckett, Dorothy (1998). "Coupling of Site-Specific DNA Binding to Protein Dimerization in Assembly of the ...

Its main target is the ATPase site of the DNA Gyrase GyrB subunit. Chemically induced dimerization Heide L (2009). "Genetic ... Vanden Broeck A, McEwen AG, Chebaro Y, Potier N, Lamour V (April 2019). "Structural Basis for DNA Gyrase Interaction with ...

Their affinity for gyrase is considerably higher than that of modern fluoroquinolones, which also target DNA gyrase but at the ... Aminocoumarin is a class of antibiotics that act by an inhibition of the DNA gyrase enzyme involved in the cell division in ... X-ray crystallography studies have confirmed binding at the ATP-binding site located on the gyrB subunit of DNA gyrase. ... Galm, Ute, Heller, Stefanie, Shapiro, Stuart, Page, Malcolm, Li, Shu-Ming, Heide, Lutz Antimicrobial and DNA Gyrase-Inhibitory ...

The antibiotic works by inhibiting the enzyme DNA gyrase. It also acts as a dopamine reuptake inhibitor and has stimulant ...

It accomplishes this by inhibiting DNA gyrase, and thereby preventing the replication of chloroplast DNA. As such it plays a ... As a DNA gyrase inhibitor, albicindin also has potential therapeutic use as an antibiotic. Its antibiotic properties were ... Hashimi, Saeed Mujahid (2019). "Albicidin, a potent DNA gyrase inhibitor with clinical potential". The Journal of Antibiotics. ... "The Phytotoxin Albicidin is a Novel Inhibitor of DNA Gyrase". Antimicrobial Agents and Chemotherapy. 51 (1): 181-187. doi: ...

"Inhibition of translesion DNA polymerase by archaeal reverse gyrase". Nucleic Acids Research. 37 (13): 4287-4295. doi:10.1093/ ... Using DNA-DNA hybridization, the total DNA-DNA relatedness of the two organisms was found to be 34.6 percent which, along with ... Reverse gyrase is theorized to keep the genome stable and prevent damage by the extreme heat. Along with the ability to fix ... Analysis of this gene demonstrated that this organism shared 97.8 percent of its DNA for this gene with the related bacterium ...

ParD forms a dimer and also regulates its own promoter (parDE). As with CcdB the toxin target is DNA gyrase. Induction of ParE ... ParD is a plasmid anti-toxin that forms a ribbon-helix-helix DNA binding structure. It stabilises plasmids by inhibiting ParE ... ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding ...

... is a bactericidal drug that inhibits bacterial DNA gyrase and topoisomerase IV. Like other quinolones and ... "Mechanism of action of quinolones against Escherichia coli DNA gyrase". Antimicrobial Agents and Chemotherapy. 37 (4): 839-45. ... fluoroquinolones the compound eradicates bacteria by interfering with DNA replication (bacterial DNA replication, transcription ...

Like other fluoroquinolones, enoxacin functions by inhibiting bacterial DNA gyrase and topoisomerase IV. The inhibition of ... "Mechanism of action of quinolones against Escherichia coli DNA gyrase". Antimicrobial Agents and Chemotherapy. 37 (4): 839-845 ... Quinolones and fluoroquinolones are bactericidal drugs, eradicating bacteria by interfering with DNA replication. ... these enzymes prevents bacterial DNA replication, transcription, repair and recombination. Recently, it was shown that Enoxacin ...

Quinolones inhibit the bacterial DNA gyrase or the topoisomerase IV enzyme, thereby inhibiting DNA replication and ... For many gram-negative bacteria DNA gyrase is the target, whereas topoisomerase IV is the target for many gram-positive ... Eukaryotic cells do not contain DNA gyrase or topoisomerase IV. Quinolones Psaty BM (December 2008). "Clinical trial design and ...

"New Antibacterial Agents Derived from the DNA Gyrase Inhibitor Cyclothialidine". Journal of Medicinal Chemistry. 47 (6): 1487- ...

Yamashiro, K.; Yamagishi, A. (2005). "Characterization of the DNA Gyrase from the Thermoacidophilic Archaeon Thermoplasma ...

Kampranis SC, Bates AD, Maxwell A (July 1999). "A model for the mechanism of strand passage by DNA gyrase". Proc. Natl. Acad. ... It appears that Hsp90 can act as a "protector" of less stable proteins produced by DNA mutations. Hsp90 is also required for ... Once in the nucleus, the GR dimerizes and binds to specific sequences of DNA and thereby upregulates the expression of GR ... Gyrase, Hsp90, Histidine Kinase, MutL) superfamily. A common binding pocket for ATP and the inhibitor geldanamycin is situated ...

Like other quinolone antibiotics, ozenoxacin targets DNA gyrase and topoisomerase IV. Its activity against bacteria with ...

... is an aminocoumarin antibacterial that inhibits the enzyme DNA gyrase. [1], http://www.drugbank.ca/drugs/DB03966. ...

It has the same mechanism of action as fluouroquinolones; it inhibits DNA gyrase, preventing DNA synthesis, gene duplication, ...

"Posttranslational modifications in microcin B17 define an additional class of DNA gyrase inhibitor". Proc Natl Acad Sci U S A. ... His work defined an origin of DNA replication that led to the development of many suicide cloning vectors still in use today. ... Finkel, S. E.; Kolter, R. (November 2001). "DNA as a nutrient: novel role for bacterial competence gene homologs". Journal of ... Kolter, R; Helinski, DR (1982). "Plasmid R6K DNA replication. II. Direct nucleotide sequence repeats are required for an active ...

Mutations in DNA gyrase are commonly associated with antibiotic resistance in P. aeruginosa. These mutations, when combined ... which protects DNA gyrase and topoisomerase IV from the effects of quinolone (fluoroquinolone) antibiotics such as ... LigDs form a subfamily of the DNA ligases. These all have a LigDom/ligase domain, but many bacterial LigDs also have separate ... September 2015). "Pel is a cationic exopolysaccharide that cross-links extracellular DNA in the Pseudomonas aeruginosa biofilm ...