A bacterial DNA transposon. A transposable element (TE or transposon) is a DNA sequence that can change its position within a ... The DNA-transposase complex then inserts its DNA cargo at specific DNA motifs elsewhere in the genome, creating short TSDs upon ... A DNA polymerase fills in the resulting gaps from the sticky ends and DNA ligase closes the sugar-phosphate backbone. This ... In bacteria, transposons can jump from chromosomal DNA to plasmid DNA and back, allowing for the transfer and permanent ...
"Bacterial genetics". Nature. Macmillan Publishers Limited. Retrieved 8 November 2015.. *^ Chen I, Dubnau D (2004). "DNA uptake ... Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge ... Natural transformation is a bacterial adaptation for DNA transfer between two cells through the intervening medium. The uptake ... Bacterial conjugation has been extensively studied in Escherichia coli, but also occurs in other bacteria such as Mycobacterium ...
... natural bacterial transformation involves the transfer of DNA from one bacterium to another and integration of the donor DNA ... Chen I; Dubnau D (March 2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): 241-249. doi:10.1038/ ... The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments ... All cells possess DNA, the hereditary material of genes, and RNA, containing the information necessary to build various ...
Setlow P (April 2007). "I will survive: DNA protection in bacterial spores". Trends in Microbiology. 15 (4): 172-80. doi: ... correctly described Clostridium botulinum as the bacterial source of the toxin. Thirty-four attendees at a funeral were ...
... , a bacterial histone-like DNA-binding protein. *Wu Hu (disambiguation), an ancient Chinese term for multiple groups in China ...
Bacterial artificial chromosomes (BACs) are circular DNA molecules, usually about 7kb in length, that are capable of holding ... The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a ... Use the enzyme DNA ligase to seal the DNA fragments into the vector. This creates a large pool of recombinant molecules. These ... Extract and purify DNA. Digest the DNA with a restriction enzyme. This creates fragments that are similar in size, each ...
C content of bacterial chromosomes by monitoring fluorescence intensity during DNA denaturation in a capillary tube". Int. J. ... "DNA-DNA hybridization determined in micro-wells using covalent attachment of DNA". Int. J. Syst. Evol. Microbiol. 50: 1095-1102 ... Euzeby, JP (1997). "List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet". Int. J. Syst. ... Palys, T; Nakamura LK; Cohan FM (1997). "Discovery and classification of ecological diversity in the bacterial world: the role ...
"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity". Science. 337 (6096): 816-821. doi:10.1126/ ... acting as a programmable DNA binding protein, to cleave DNA at a site of interest.[1][2] ... Exposing various ectopic DNA loci to natural lncRNAs can help show the effects of lncRNAs on gene expression and chromatin ... and inability to distinguish lncRNA function from other confounding factors like cryptically encoded peptides or functional DNA ...
DNA vector, episomal, lasting expression, immunogenic Bacterial vector species (bacterial minicells can carry plasmids, siRNAs ... and/or chromatic or DNA modification.[7][8][9] In the context in which the phenomenon was first studied, small RNA was found to ... including repetitive DNA and transposons.[44] However, the biogenesis of piRNAs is also the least well understood.[45] piRNAs ... like DNA, is a double stranded series of nucleotides. If the mechanism didn't use dsRNAs, but only single strands, there would ...
Wilkinson A, Day J, Bowater R (2001). "Bacterial DNA ligases". Mol. Microbiol. 40 (6): 1241-8. PMID 11442824. doi:10.1046/j. ... "A newly identified DNA ligase of Saccharomyces cerevisiae involved in RAD52-independent repair of DNA double-strand breaks". ... Chambon P, Weill JD, Mandel P (1963). "Nicotinamide mononucleotide activation of new DNA-dependent polyadenylic acid ...
Other NAD-dependent enzymes include bacterial DNA ligases, which join two DNA ends by using NAD+ as a substrate to donate an ... Wilkinson A, Day J, Bowater R (2001). "Bacterial DNA ligases". Mol. Microbiol. 40 (6): 1241-8. doi:10.1046/j.1365-2958.2001. ... as well as acting as a substrate for bacterial DNA ligases and a group of enzymes called sirtuins that use NAD+ to remove ... "A newly identified DNA ligase of Saccharomyces cerevisiae involved in RAD52-independent repair of DNA double-strand breaks". ...
The DNA sequence could be distinguished by the specific modulating effect of the four bases on the ionic current through the ... Loman, N.J.; Quick, J.; Simpson, J.T. (2015). "A complete bacterial genome assembled de novo using only nanopore sequencing ... His work led to a novel method of DNA sequencing and a more complete understanding of the role of membranes in the origin of ... The first publications appeared in 2015, one of which used the MinION to sequence E. coli DNA with 99.4% accuracy relative to ...
Jayaraman R (2008). "Bacterial persistence: some new insights into an old phenomenon" (PDF). J Biosci. 33 (5): 795-805. doi: ... As a result of the DNA damage, yeast persisters are also enriched for random genetic mutations that occurred prior to the ... Bacterial multidrug or antibiotic tolerance poses medically important challenges. It is largely responsible for the inability ... Yeast persisters are triggered in a small subset of unperturbed exponentially growing cells by spontaneously occurring DNA ...
Other NAD-dependent enzymes include bacterial DNA ligases, which join two DNA ends by using NAD+ as a substrate to donate an ... Wilkinson A, Day J, Bowater R (2001). "Bacterial DNA ligases". Mol. Microbiol. 40 (6): 1241-8. doi:10.1046/j.1365-2958.2001. ... This contrasts with eukaryotic DNA ligases, which use ATP to form the DNA-AMP intermediate. In recent years, NAD+ has also been ... as well as acting as a substrate for bacterial DNA ligases and a group of enzymes called sirtuins that use NAD+ to remove ...
Chen I, Dubnau D. DNA uptake during bacterial transformation. Nat. Rev. Microbiol.. 2004, s. 241-9. DOI:10.1038/nrmicro844. ... Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA.. ... V roce 1972 Paul Berg vytvořil první molekuly rekombinantní DNA spojením DNA opičího viru SV40 a lambda viru.[5] V roce 1973 ... která pak může existovat jako extrachromozomální DNA nebo se začlenit do jejich genomu. Do živočišných buněk se DNA může vnášet ...
Wilkinson A, Day J, Bowater R «Bacterial DNA ligases». Mol. Microbiol., 40, 6, 2001, pàg. 1241-8. DOI: 10.1046/j.1365-2958.2001 ... d'un extrem del DNA. Aquest intermediari és seguidament atacat pel grup hidroxil 3' de l'altre extrem de DNA, formant un nou ... A newly identified DNA ligase of Saccharomyces cerevisiae involved in RAD52-independent repair of DNA double-strand breaks». ... Chambon P, Weill JD, Mandel P «Nicotinamide mononucleotide activation of new DNA-dependent polyadenylic acid synthesizing ...
Quinolones inhibit the bacterial DNA gyrase or the topoisomerase IV enzyme, thereby inhibiting DNA replication and ... Castora, F. J.; Vissering, F. F.; Simpson, M. V. (September 1983). "The effect of bacterial DNA gyrase inhibitors on DNA ... Structure of bacterial DNA gyrase complexed with DNA and two ciprofloxacin molecules (green) ... Naphthyridone and quinolone classes of antibiotics prevent bacterial DNA replication by inhibition of DNA unwinding events, and ...
... self-replicating DNA molecules that are separate from the bacterial chromosome.[10] Plasmids can carry genes responsible for ... Transformation is a bacterial process for transferring DNA from one cell to another, and is apparently an adaptation for ... allows for efficient recombinational repair of DNA damage [13] and a greater range of genetic diversity by combining the DNA of ... Under stressful environmental conditions that cause DNA damage, some species of archaea aggregate and transfer DNA between ...
"DNA Research. 15 (6): 387-396. doi:10.1093/dnares/dsn027. ISSN 1340-2838. PMC 2608843 . PMID 18940874.. ... Additionally, some bacterial initiation regions, such as rpsA in E.coli completely lack identifiable SD sequences.[4] ... Bacterial mRNA are usually polycistronic and contain multiple ribosome binding sites. Translation initiation is the most highly ... Mostly, RBS refers to bacterial sequences, although internal ribosome entry sites (IRES) have been described in mRNAs of ...
After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these ... A ZFP is itself a DNA-binding protein used in the construction of custom DNA-binding domains that bind to a desired DNA ... including some that detect protein-DNA interactions or DNA-DNA interactions, as well as methods that use different host ... It may be of note that the methylation activity of certain E. coli DNA methyltransferase proteins may interfere with some DNA- ...
Often other DNA, such as bacterial DNA, will be present in the original sample. To avoid contamination it is necessary to take ... The first phase is bacterial putrefaction, which is estimated to cause a 15-fold degradation of DNA. Phase 2 is when bone ... The best time to extract DNA from a fossil is when it is freshly out of the ground as it contains six times the DNA when ... Nuclear DNA in specific is used over mitochondrial and chloroplast DNA because of its faster mutation rate as well as its ...
Mitochondrial DNA resembles bacterial DNA. If bacteria triggers leukocytes, mitochondrial DNA may do the same. When confronted ... mitochondrial DNA is the leading cause of severe inflammation due to a massive amount of Mitochondrial DNA that leaks into the ... 2014). "Mitochondrial DNA neutrophil extracellular traps are formed after trauma and subsequent surgery". Journal of Critical ... Simple intestinal obstruction causes bacterial translocation in man. Arch Surg 1989; 124: 699-701. PMID 2730322 McIlroy DJ; et ...
Natural transformation is a common bacterial adaptation for DNA transfer that employs numerous bacterial gene products. For a ... 496-8. ISBN 0-387-24144-2. Chen I, Dubnau D (2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): ... The DNA-uptake process of naturally competent V. cholerae involves an extended competence-induced pilus and a DNA binding ... V. cholerae has been used in discoveries of many bacterial small RNAs. Using sRNA-Seq and Northern blot candidate sRNAs were ...
doi:10.1111/j.1472-765x.1991.tb00606.x. Day, edited by John C. Fry, Martin J. (1990). Bacterial Genetics in Natural ... "Detection and quantification of Streptomyces violaceolatus plasmid DNA in soil". Letters in Applied Microbiology. 13 (4): 193- ...
specifically identified bacterial DNA as the underlying component of the lysate that elicited the response. Then, in 1995 Krieg ... Bauer, S; Wagner, H (2002). "Bacterial CpG-DNA licenses TLR9". Current topics in microbiology and immunology. Current Topics in ... demonstrated that the CpG motif within bacterial DNA was responsible for the immunostimulatory effects and developed synthetic ... "CpG motifs in bacterial DNA trigger direct B-cell activation". Nature. 374 (6522): 546-9. doi:10.1038/374546a0. PMID 7700380. ...
Natural bacterial transformation involves the transfer of DNA from one bacterium to another through the surrounding medium. ... The genome of S. pneumoniae is a closed, circular DNA structure that contains between 2.0 and 2.1 million base pairs depending ... Competence in S. pneumoniae is induced by DNA-damaging agents such as mitomycin C, fluoroquinolone antibiotics (norfloxacin, ... The ability of S. pneumoniae to repair the oxidative DNA damages in its genome, caused by this host defense, likely contributes ...