DNA-(apurinic or apyrimidinic site) lyase is an enzyme that in humans is encoded by the APEX1 gene. Apurinic/apyrimidinic (AP) ... "Abasic site recognition by two apurinic/apyrimidinic endonuclease families in DNA base excision repair: the 3' ends justify the ... sites (also called "abasic sites") occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by ... "Isolation of cDNA clones encoding a human apurinic/apyrimidinic endonuclease that corrects DNA repair and mutagenesis defects ...
... dna ligases MeSH D08.811.074.750 - DNA-(apurinic or apyrimidinic site) lyase MeSH D08.811.074.781 - muts dna mismatch-binding ... DNA-(apurinic or apyrimidinic site) lyase MeSH D08.811.520.241.300 - hydro-lyases MeSH D08.811.520.241.300.050 - aconitate ... site-specific dna-methyltransferase (adenine-specific) MeSH D08.811.913.555.500.350.850 - site-specific dna methyltransferase ( ... deoxyribodipyrimidine photo-lyase MeSH D08.811.074.249 - dna glycosylases MeSH D08.811.074.249.500 - DNA-formamidopyrimidine ...
DNA-(apurinic or apyrimidinic site) lyase EC 4.2.99.19: transferred entry, now EC 4.4.1.23 EC 4.2.99.20: 2-succinyl-6-hydroxy-2 ... pectin lyase EC 4.2.2.11: poly(a-L-guluronate) lyase EC 4.2.2.12: xanthan lyase EC 4.2.2.13: exo-(1-4)-α-D-glucan lyase EC 4.2. ... pectate lyase EC 4.2.2.3: poly(b-D-mannuronate) lyase EC 4.2.2.4: chondroitin ABC lyase EC 4.2.2.5: chondroitin AC lyase EC 4.2 ... oligogalacturonide lyase EC 4.2.2.7: heparin lyase EC 4.2.2.8: heparin-sulfate lyase EC 4.2.2.9: pectate disaccharide-lyase EC ...
... apurinic/apyrimidinic) lyase activity introduces nicks in the DNA strand, cleaving the DNA backbone by beta-delta elimination ... phosphodiester bonds of the resulting apurinic/apyrimidinic site (AP lyase activity;EC 4.2.99.18). Fpg has a preference for ... is a DNA-binding domain found in DNA glycosylase/AP lyase enzymes, which are involved in base excision repair of DNA damaged by ... These enzymes are primarily from bacteria, and have both DNA glycosylase activity EC 3.2.2.- and AP lyase activity EC 4.2.99.18 ...
In enzymology, DNA-(apurinic or apyrimidinic site) lyase, also referred to as DNA-(apurinic or apyrimidinic site) 5'- ... Several names for DNA AP lyase include: AP lyase; AP endonuclease class I; endodeoxyribonuclease (apurinic or apyrimidinic); ... DNA-(apurinic or apyrimidinic site) lyase activity". HumanCyc. SRI International. Retrieved March 9, 2015. Müller TA, Andrzejak ... AP site-DNA 5'-phosphomonoester-lyase; and X-ray endonuclease III. Since DNA AP lyase is a class of structures who have ...
The excision of the bases causes the formation of an apurinic/ apyrimidinic (AP site) gap. These gap sites are mutagenic in ... namely polymerase and dRP lyase. DNA ligase is used to seal the fragments after dRP excision causes the formation of 5'PO4 that ... MUTYH (mutY DNA glycosylase) is a human gene that encodes a DNA glycosylase, MUTYH glycosylase. It is involved in oxidative DNA ... The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine ...
... apurinic/apyrimidinic site), also known as an abasic site, is a location in DNA (also in RNA but much less likely) that has ... see DNA structure). In alternative fashion, bifunctional glycosylase-lyases can cleave the AP site, leaving a 5' phosphate ... In a given mammalian cell, 5000-10,000 apurinic sites are estimated to form per day. Apyrimidinic sites form at a rate roughly ... It has been estimated that under physiological conditions 10,000 apurinic sites and 500 apyrimidinic may be generated in a cell ...
... creating an apurinic/apyrimidinic site, commonly referred to as an AP site. In this Figure, the 8-OHdG is left in the DNA, ... Levin, Joshua D; Demple, Bruce (1990). "Analysis of class II (hydrolytic) and class I (beta-lyase) apurinic/apyrimidinic ... In the mature oocyte, about 40% of its CpG sites in DNA are methylated. While somatic cells of mammals have three main DNA ... The mouse sperm genome is 80-90% methylated at its CpG sites in DNA, amounting to about 20 million methylated sites.[citation ...
... creating an apurinic/apyrimidinic site, commonly referred to as an AP site. This is accomplished by flipping the damaged base ... Aguis, F.; Kapoor, A; Zhu, J-K (2006). "Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation". Proc. ... Uracil-DNA glycosylases are DNA repair enzymes that excise uracil residues from DNA by cleaving the N-glycosydic bond, ... Lindahl, T. (1986). "DNA Glycosylases in DNA Repair". Mechanisms of DNA Damage and Repair. 38: 335-340. doi:10.1007/978-1-4615- ...
Apurinic/apyrimidinic (AP) endonuclease is an enzyme that is involved in the DNA base excision repair pathway (BER). Its main ... Class I AP endonucleases (EC 4.2.99.18) cleave 3′ to AP sites by a β-lyase mechanism, leaving an unsaturated aldehyde, termed a ... Levin, Joshua D; Demple, Bruce (1990). "Analysis of class II (hydrolytic) and class I (beta-lyase) apurinic/apyrimidinic ... This extreme kinking forces the baseless portion of DNA into APE1's active site. This active site is bordered by Phe266, Trp280 ...
5'-deoxyribose-5-phosphate lyase activity. • transcription factor binding. • DNA-(apurinic or apyrimidinic site) endonuclease ... DNA unwinding involved in DNA replication. • transcription, DNA-templated. • transcription by RNA polymerase II. • nuclear ... regulation of transcription, DNA-templated. • positive regulation of transcription, DNA-templated. • positive regulation of ... DNA binding. • GO:0001077, GO:0001212, GO:0001213, GO:0001211, GO:0001205 DNA-binding transcription activator activity, RNA ...
found that HGMA2 protein can efficiently cleave DNA containing apurinic/apyrimidinic (AP) sites (is an AP lyase). In addition, ... showed that overexpression of HMGA2 delayed the release of DNA-PKcs (needed for non-homologous end joining DNA repair) from ... "HMGA2 exhibits dRP/AP site cleavage activity and protects cancer cells from DNA-damage-induced cytotoxicity during chemotherapy ... Zha L, Wang Z, Tang W, Zhang N, Liao G, Huang Z (2012). "Genome-wide analysis of HMGA2 transcription factor binding sites by ...
"Coordination of Steps in Single-nucleotide Base Excision Repair Mediated by Apurinic/Apyrimidinic Endonuclease 1 and DNA ... Some lesions, such as oxidized or reduced AP sites, are resistant to pol β lyase activity and, therefore, must be processed by ... MBD4 protein binds preferentially to fully methylated CpG sites and to the altered DNA bases at those sites. These altered ... This was the most frequent DNA repair abnormality found among the 8 DNA repair genes tested. NEIL1 was also one of six DNA ...
... only a single nucleotide is added and DNA Polymerase acts as a lyase to excise the AP site. In LP-BER, several bases are ... "Reconstitution of proliferating cell nuclear antigen-dependent repair of apurinic/apyrimidinic sites with purified human ... the DNA ligase becomes associated with the DNA, locates a nick and catalyzes a reaction at the 5' phosphate site of the DNA ... First, a DNA glycosylase cleaves the N-glycosidic bond, releasing the damaged base and creating an AP site- a site that lacks a ...