Peters, K.; Richards, F. M. (1977). "Chemical Cross-Linking: Reagents and Problems in Studies of Membrane Structure". Annual ... Richards FM, Knowles JR (1968). "Glutaraldehyde as a protein cross-linking reagent". Journal of Molecular Biology. 37 (1): 231- ... In the 1970s, with a succession of students and postdocs, the lab developed a series of chemical, photochemical, and cross-link ... doi:10.1016/S0021-9258(19)67984-6. Quiocho FA, Richards FM (1964). "Intermolecular cross linking of a protein in the ...
Futaki S. and Kitagawa K. (1994). "Peptide-Unit Assembling Using Disulfide Cross-Linking - a New Approach for Construction of ... doi:10.1016/0040-4039(94)88040-9. "Special Reagents for Thiol Groups". Aldrichimica Acta. 4 (3): 33-46. Thalmann A., Oertle K. ... Reagents for organic chemistry, All stub articles, Organic compound stubs). ...
This reagent is mainly used to form intramolecular crosslinks and preparation of polymers from monomers. It is ideal for ... In a study on blood substitutes, DSS was shown to cross-link Hemoglobin intramolecularly, yielding a relatively stable protein ... Disuccinimidyl suberate (DSS) is a six-carbon lysine-reactive non-cleavable cross-linking agent. It consists of functional ... It is membrane permeable, therefore permitting intracellular cross-linking, has high purity, is non-cleavable, and is water- ...
The dicarboxylic acids, especially the small and linear ones, can be used as crosslinking reagents. Dicarboxylic acids where ... The PubChem links gives access to more information on the compounds, including other names, ids, toxicity and safety. Acids ... Pages containing links to subscription-or-libraries content, Articles with short description, Short description is different ...
Sulfur forms cross-linking bridges between sections of polymer chains which affects the mechanical and electronic properties. ... The cure package consists of various reagents that modify the kinetics and chemistry of crosslinking. These include accelerants ... They act to boost the cure speed and increase cross-link density, but also shorten the induction time, which can lead to ... Ultimately, activators promote the efficient use of sulfur to give a high density of cross-links. Due to the low solubility of ...
Selective crosslinking of proteins to CDAP-activated polysaccharides. Vaccine. 18:1273, 2000. Shafer, D. E, J. K. Inman, and A ... John Inman, Lees learned bioconjugation, the linking together of biologically relevant molecules to increase their utility. In ... Activation of soluble polysaccharides with CDAP for use in protein-polysaccharide conjugate vaccines and immunological reagents ... Versatile and efficient synthesis of protein-polysaccharide conjugate vaccines using aminooxy reagents and oxime chemistry. ...
This re-forming of the hydrogen bonds, known as cross linking, is the reason for wrinkles, or creases, in the fabric. DMEU ... A new hydrophilic crosslinking reagent for anti-crease finishing of cotton fabrics". Carbohydrate Research. 486: 107783. doi: ... Masters Thesis Petersen, H. (1968). "Reaction Mechanisms, Structure, and Properties of Methylol Compounds in Cross-Linking ... As a result of these health concerns there are alternate areas of research in ionic crosslinking compounds as opposed to DMEU ...
An in-vitro cross-linking method is PICUP (photo-induced cross-linking of unmodified proteins). Typical reagents are ammonium ... UV cross-linking). Branching (polymer chemistry) Cross-linked enzyme aggregate Cross-linked polyethylene (PEX) Crosslinking of ... Chemical covalent cross-links are stable mechanically and thermally. Therefore, cross-linked products like car tires cannot be ... Low cross-link densities increase the viscosities of polymer melts. Intermediate cross-link densities transform gummy polymers ...
In addition, the cross-linked protein yield is very low due to the multifunctionality of the cross-linking reagents. The ... Photo-Induced Cross-Linking of Unmodified Proteins (PICUP) is a protein cross-linking method by visible light irradiation of a ... Cross-linking methods developed prior to PICUP, including the use of physical, oxidative, and chemical cross-linkers, often ... The techniques in the 20th century were not sufficient to be applied to cross-link fast and transient changes of these proteins ...
Cross-linking the protein precursor of marine mussel adhesives: bulk measurements and reagents for curing. Langmuir 20 (9), ... The Fe(L-DOPA3) complex can itself account for much cross-linking and cohesion in mussel plaque, but in addition the iron ... 1999;80(1). Sever M.J.; Weisser, J.T.; Monahan, J.; Srinivasan, S.; Wilker, J.J. (2004) Metal-mediated cross-linking in the ... Proteins in the oothecal foam of the mantis are cross-linked covalently by small molecules related to L-DOPA via a tanning ...
... the experimenter may apply SDS-PAGE after first treating the intact complex with chemical cross-link reagents. Some ...
... thiol modification and cross-linking reagents". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1458 (2-3): 443-456. doi: ... This link is tenuous, however, as the overall structure of flagellar motors is far more complex than that of the FO particle ... Cross RL, Taiz L (January 1990). "Gene duplication as a means for altering H+/ATP ratios during the evolution of FOF1 ATPases ... Cross RL, Müller V (October 2004). "The evolution of A-, F-, and V-type ATP synthases and ATPases: reversals in function and ...
... thiol modification and cross-linking reagents". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1458 (2-3): 443-56. doi: ... All articles with dead external links, Articles with dead external links from October 2022, Articles with permanently dead ... "ATP synthase protein 8". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB).[permanent dead link] "MT-ATP8 mitochondrially ... linked together by a central stalk and a peripheral stalk. As an A subunit, MT-ATP8 is contained within the non-catalytic, ...
... is used as a crosslinker for the synthesis of cleavable photo-cross-linking reagent. Mono-BOC-cystamine is ... Peter E Nielsen, John B Hansen and Ole Buchard (1984). "Photochemical cross-linking of protein and DNA in chromatin". Biochem J ... All articles with dead external links, Articles with dead external links from February 2018, Articles with permanently dead ... Biotin-Peg2-Amine Biotin-Peg2-Maleimide Biotin-Peg3-Amine Biotin-Peg4-NHS Mono BOC Cystamine[permanent dead link], Anachem ...
"Methyl 4-mercaptobutyrimidate as a cleavable cross-linking reagent and its application to the Escherichia coli 30S ribosome". ... 2-Iminothiolane is a cyclic thioimidate compound also known as Traut's reagent. It is a thiolating reagent that reacts with ... Thus it allows for crosslinking or labeling of molecules such as proteins through use of disulfide or thioether conjugation. It ... "Traut's Reagent Instructions" (PDF). Thermo Scientific. Retrieved 2015-07-08. (Chemical articles with multiple compound IDs, ...
The third category involves cross-linking of enzyme aggregates or crystals, using a bifunctional reagent, to prepare carrier- ... In biochemistry, a cross-linked enzyme aggregate is an immobilized enzyme prepared via cross-linking of the physical enzyme ... such as cross-linked enzyme crystals (CLECs) and cross-linked enzyme aggregates (CLEAs) that offer the advantages of highly ... The use of cross-linked enzyme crystals (CLECs) as industrial biocatalysts was pioneered by Altus Biologics in the 1990s. CLECs ...
It works by cross-linking DNA. Testing for cyanuric acid concentration is commonly done with a turbidometric test, which uses a ... reagent, melamine, to precipitate the cyanuric acid. The relative turbidity of the reacted sample quantifies the CYA ... dead link 8 April 2018) Panuwet P, Wade EL, Nguyen JV, Montesano MA, Needham LL, Barr DB. Quantification of cyanuric acid ... Because of its trifunctionality, CYA is a precursor to crosslinking agents, especially for polyurethane resins and ...
It is also used as a cross-linking agent to cross-link chitosan membranes, and also improves the membrane's integrity. " ... Suberoyl chloride is used as a reagent to synthesize hydroxyferrocifen hybrid compounds that have antiproliferative activity ...
... is used as a solubilizer and cross-linking agent in polymer chemistry. Glyoxal is a valuable building block in organic ... A convenient form of the reagent for use in the laboratory is its bis(hemiacetal) with ethylene glycol, 1,4-dioxane-2,3-diol. ... a DNA cross-link precursor. Int J Biol Macromol. 2017 May;98:664-675. doi: 10.1016/j.ijbiomac.2017.01.140. Epub 2017 Feb 10. ... These adducts may then produce DNA crosslinks. Glycation of DNA may also lead to mutation, breaks in DNA and cytotoxicity. In ...
Seven residues and longer are sufficient for cross-linking, leading to precipitation of the glycans with the Yariv ... 3-linked Gal units to make aggregates with the Yariv reagent. After translation, the AGP protein backbones are highly decorated ... The structure of the AG glycans consists of a backbone of β-1,3 linked galactose (Gal), with sidechains of β-1,6 linked Gal and ... The AG found in AGPs is of type II (type II AGs) - that is, a galactan backbone of (1-3)-linked β-D-galactopyranose (Galp) ...
... and are used as crosslinking reagents in thermoset polymer chemistry. Compounds containing a maleimide group linked with ... Maleimides linked to polyethylene glycol chains are often used as flexible linking molecules to attach proteins to surfaces. ... Maleimides linked to rubber chains are often used as flexible linking molecules to reinforce rubber in tires. The double bond ... see SMCC reagent). Only a handful of natural maleimides - exemplified by the cytotoxic showdomycin from Streptomyces ...
Enhanced cross-linking and immunoprecipitation followed by high-throughput sequencing) sCLIP (Simple cross-linking and ... Long-wavelength UV and SDA reagents are first supplied to living cells to crosslink protein residues with RNA by forming amide ... Photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation) iCLIP (Individual nucleotide-resolution cross- ... CLIP analyzes protein interactions with RNA by combining UV cross-linking and immunoprecipitation. CLIP-based techniques are ...
Synthesis of extended equilibrium transfer alkylating cross-link reagents and their use in the formation of macrocycles Stephen ... applicable for reactions betwixt allylic compounds and reagents like SOCl2. The synthetic utility can be extended to ...
... much less toxic than glutaraldehyde and many other commonly used synthetic cross-linking reagents. Furthermore, genipin can be ... Genipin is an excellent natural cross-linker for proteins, collagen, gelatin, and chitosan cross-linking. It has a low acute ... link) Zhang, CY; Parton, LE; Ye, CP; Krauss, S; Shen, R; Lin, CT; Porco Jr, JA; Lowell, BB (2006). "Genipin inhibits UCP2- ...
... cross-linking reagents MeSH D27.720.470.410.360 - intercalating agents MeSH D27.720.470.410.505 - luminescent agents MeSH ... reagent strips MeSH D27.720.470.410.690 - reducing agents MeSH D27.720.470.410.700 - sulfhydryl reagents MeSH D27.720.470.410. ... indicators and reagents MeSH D27.720.470.410.080 - affinity labels MeSH D27.720.470.410.080.600 - photoaffinity labels MeSH ... D27.720.470.410.505.500 - fluorescent dyes MeSH D27.720.470.410.650 - radiopharmaceuticals MeSH D27.720.470.410.680 - reagent ...
... and by using cross-linking reagents within some experiments, a fragile complex was identified called the pre-loading complex ( ... Dpb11 and Sld2 interact with Polymerase ɛ and cross-linking experiments have indicated that Dpb11 and Polymerase ɛ ... However, the two DNA molecules will remain linked together. This issue is handled by decatenation of the two DNA molecules by a ... The importance of this direct link between the helicase and the leading-strand polymerase is underscored by results in cultured ...
Photolithography can also be used to cross-link cell-seeded photo-polymerizable ECM for three-dimensional studies. Using ECM ... This approach uses less energy and has high throughput, but has large reagent consumption and gas bubbles can form inside the ... Other drawbacks of conventional PCR is the high consumption of expensive reagents, preference for amplifying short fragments, ... Digital PCR eliminates sample/reagent surface adsorption and contamination by carrying out PCR in microdroplets or ...
... performed by creating crosslinks between the two molecules of interest and can require a wide variety of different reagents ... Enzyme linked immunosorbent assays are used most commonly as diagnostic tests to detect HIV antibodies in blood samples to test ... The resulting product will not need purification to remove the enzyme because it will remain linked to the beads in the column ... Proteins are polymers that are made up of amino acid chains linked with peptide bonds. They have four distinct levels of ...
... improve the cross-linking efficiency. If the cross-linking is done in vivo, within living cells, these must be exposed to the ... that involved the use of moderately reactive bifunctional reagent, commonly attached to free amino groups. However, ... In addition, photochemical cross-linking does not interfere with the antibodies recognition whilst the former does. But photo- ... Traditionally, the recognition of protein-protein interactions was carried out through chemical cross-linking, ...
... synthesis and a component in many car airbag systems Psoralen combined with ultraviolet radiation causes DNA cross-linking and ... Sodium azide, an azide salt that is a common reagent in organic ... Sex-Linked Recessive Lethal Test - Males from a strain with ... These ROS may result in the production of many base adducts, as well as DNA strand breaks and crosslinks. Deaminating agents, ... The first mutagens to be identified were carcinogens, substances that were shown to be linked to cancer. Tumors were described ...