"Genome sequences of Escherichia coli B strains REL606 and BL21(DE3)". Journal of Molecular Biology. 394 (4): 644-52. doi: ... E. coli strain BL21 and BL21(DE3) are two strains commonly used for protein production. As members of the B lineage, they lack ... The techniques for overexpression in E. coli are well developed and work by increasing the number of copies of the gene or ... For example, common hosts are bacteria (such as E.coli, B. subtilis), yeast (such as S.cerevisiae) or eukaryotic cell lines. ...
... coli factor-dependent terminators. Other termination factors discovered in E. coli include Tau and nusA. Rho-dependent ... terminators were first discovered in bacteriophage genomes. A Rho factor acts on an RNA substrate. Rho's key function is its ... In Escherichia coli, it is a ~274.6 kD hexamer of identical subunits. Each subunit has an RNA-binding domain and an ATP- ...
The E. coli genome encodes three paralogues, YbhL, YbhM and YccA. Distant homologues found in Drosophilia melanogaster and the ... the YccA protein of Escherichia coli and the YetJ protein of Bacillus subtilis. These proteins are about 200-250 residues in ...
The first living organism with 'artificial' expanded DNA code was presented in 2014; the team used E. coli that had its genome ... A simple genome might also work as a "chassis genome" that could be enlarged quickly by gene inclusion created for particular ... May 2019). "Total synthesis of Escherichia coli with a recoded genome". Nature. 569 (7757): 514-518. Bibcode:2019Natur.569.. ... To increase genome modularity, the practice of genome refactoring or improving "the internal structure of an existing system ...
It was reclassified in the genus Shimwellia based on phylogenetic analyses of its genome sequence. The genome of S. blattae is ... Although it is related to human pathogens, including Escherichia coli, S. blattae is not pathogenic to humans. It is notable ... It has 56.5% GC content, significantly higher than E. coli. Genomic analysis suggests that the ability of S. blattae to ... Brzuszkiewicz, E.; Waschkowitz, T.; Wiezer, A.; Daniel, R. (27 July 2012). "Complete Genome Sequence of the B12-Producing ...
position: 156E → K; loss of activity Nichols BP, Green JM (August 1992). "Cloning and sequencing of Escherichia coli ubiC and ... "KEGG PATHWAY: Ubiquinone and other terpenoid-quinone biosynthesis - Reference pathway". www.genome.jp. Retrieved 2021-04-09. " ... Siebert M, Severin K, Heide L (1994). "Formation of 4-hydroxybenzoate in Escherichia coli: characterization of the ubiC gene ... "EC 4.1.3.40". Siebert M, Severin K, Heide L (April 1994). "Formation of 4-hydroxybenzoate in Escherichia coli: characterization ...
"Genome evolution and adaptation in a long-term experiment with Escherichia coli". Nature. 461 (7268): 1243-7. Bibcode:2009Natur ... Even if an organism is used to express the gene of interest, by mutagenising only that gene the rest of the organism's genome ... Evolution of multiple functions for ebg enzyme in E. coli". Genetics. 89 (3): 453-65. doi:10.1093/genetics/89.3.453. PMC ... Bacher, J. M.; Ellington, A. D. (2001). "Selection and characterization of Escherichia coli variants capable of growth on an ...
Bonocora RP, Smith C, Lapierre P, Wade JT (October 2015). "Genome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, ... Hunt TP, Magasanik B (December 1985). "Transcription of glnA by purified Escherichia coli components: core RNA polymerase and ... v t e (Protein pages needing a picture, Escherichia coli genes, All stub articles, Gene stubs). ... coli σ54 has since been shown to play important regulatory roles in a variety of other cellular processes. Similarly, σ54 ...
The resulting double stranded DNA is then incorporated into E.coli plasmids. Each bacterium now contains one unique cDNA and is ... The goal of the Cancer Genome Anatomy Project Genome Annotation Initiative (CGAP-GAI) is to discover and catalogue single ... on Cancer Cancer Genome Project at the Wellcome Trust Sanger Institute International Cancer Genome Consortium The Cancer Genome ... Integration of cytogenetic and physical maps of the human genome Generate a clone repository of BAC clones across the genome ...
Its genome consists of linear double-stranded DNA, with repeats at either end. The phage is covered by a protective protein ... The T2 phage can quickly turn an E. coli cell into a T2-producing factory that releases phages when the cell ruptures. ... The first phages that were studied in detail included seven that commonly infect E. coli. They were named Type 1 (T1), Type 2 ( ... Enterobacteria phage T2 is a virus that infects and kills E. coli. It is in the genus Tequatrovirus, and the family Myoviridae ...
The genome sequences reveal much diversity in bacteria. Analysis of over 2000 Escherichia coli genomes reveals an E. coli core ... On the other hand, the core genome has remain static since 2012. Currently, the E. coli pan-genome is composed of about 90,000 ... there is relatively little variation in genome size when compared with the genome sizes of other major groups of life. Genome ... The descendant small genome content depends on the content of chromosomal deletions that occur in the early stages of genome ...
Human AOX1 genome location and AOX1 gene details page in the UCSC Genome Browser. Wang AG, Yoon SY, Oh JH, et al. (2006). " ... 2009). "Purification and mechanism of human aldehyde oxidase expressed in Escherichia coli". Drug Metab. Dispos. 37 (12): 2393- ... Bonaldo MF, Lennon G, Soares MB (1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome ...
"Entrez Gene: CALML3 calmodulin-like 3". Human CALML3 genome location and CALML3 gene details page in the UCSC Genome Browser. ... 1993). "Characterization of the human calmodulin-like protein expressed in Escherichia coli". Biochemistry. 31 (51): 12826-32. ... 2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome ...
It was discovered using a computational screen of the E. coli genome. Subsequent characterisation of ISO61 region has revealed ... "A bioinformatics based approach to discover small RNA genes in the Escherichia coli genome". Bio Systems. 65 (2-3): 157-177. ... The ISO61 (IsrA) RNA is a bacterial non-coding RNA that is found between the abgR and ydaL genes in Escherichia coli and ...
Escherichia coli is an example of a species with an open pangenome. Any E. coli genome size is in the range of 4000-5000 genes ... The cloud genome consists of those gene families shared by a minimal subset of the genomes in the pangenome, it includes ... 95% of the genomes, respectively. The core genome size and proportion to the pangenome depends on several factors, but it is ... More generally, it is the union of all the genomes of a clade. The pan-genome can be broken down into a "core pangenome" that ...
The number of two-component systems present in a bacterial genome is highly correlated with genome size as well as ecological ... "Structure of the Escherichia coli response regulator NarL". Biochemistry. 35 (34): 11053-61. CiteSeerX 10.1.1.580.6078. doi: ... Genome Biology. 12 (10): R99. doi:10.1186/gb-2011-12-10-r99. PMC 3333781. PMID 21992415. Baikalov I, Schröder I, Kaczor- ... they also appear much more rarely in the genomes of some archaea, yeasts, filamentous fungi, and plants. Two-component systems ...
... coli O104 genome and attributed the strain as a category of Shiga toxin-producing enteroaggregative Escherichia coli (STpEAEC ... Escherichia coli O104:H4 is an enteroaggregative Escherichia coli strain of the bacterium Escherichia coli, and the cause of ... "BGI Sequences Genome of the Deadly E. coli in Germany and Reveals New Super-Toxic Strain". BGI. 2011-06-02. Archived from the ... E. coli O104 is a Shiga toxin-producing E. coli (STEC). The toxins cause illness and the associated symptoms by sticking to the ...
Human APC genome location and APC gene details page in the UCSC Genome Browser. (All articles with dead external links, ... Adenomatous polyposis coli (APC) also known as deleted in polyposis 2.5 (DP2.5) is a protein that in humans is encoded by the ... The (Adenomatous Polyposis Coli) APC protein normally builds a "destruction complex" with glycogen synthase kinase 3-alpha and ... Rosenberg MM, Yang F, Giovanni M, Mohn JL, Temburni MK, Jacob MH (June 2008). "Adenomatous polyposis coli plays a key role, in ...
"BGI Sequences Genome of the Deadly E. coli in Germany and Reveals New Super-Toxic Strain". BGI. 2 June 2011. Archived from the ... coli and enterohemorrhagic E. coli. The strain has a number of virulence genes typical of enteroaggregative E. coli, including ... "Escherichia coli O104:H4 Pathogenesis: An Enteroaggregative E. coli/Shiga Toxin-Producing E. coli Explosive Cocktail of High ... "EU increases E coli compensation offer - RTÉ News". Rte.ie. 20 October 2010. Retrieved 9 June 2011. "German E. coli O104:H4 ...
Sequencing of the M. pneumoniae genome in 1996 revealed it is 816,394 bp in size. The genome contains 687 genes that encode for ... The table above depicts the mean path length for the metabolomes of M. pneumoniae, E. coli, L. lactis, and B. subtilis. This ... In addition to the potential for loss of pathway function, the reduced genome of M. pneumoniae outright lacks a number of ... This is in contrast to another model organism, Escherichia coli, in which only 15% of its metabolic enzymes are essential. In ...
"Complete Genome Sequence of Crohn's Disease-Associated Adherent-Invasive E. coli Strain LF82". PLOS ONE. Public Library of ... "Reconstructing Viral Genomes from the Environment Using Fosmid Clones: The Case of Haloviruses". PLOS ONE. Public Library of ... These were later developed into the first widespread genome editing tool, CRISPR-Cas9. Mojica was born in Elche, Spain, on 5 ... "Target Motifs Affecting Natural Immunity by a Constitutive CRISPR-Cas System in Escherichia coli". PLOS ONE. Public Library of ...
The genome of S. flexneri and Escherichia coli are nearly indistinguishable at the species level. S. flexneri has a circular ... It is smaller than that of E. coli but the genes are similar. S. flexneri has about 4,084 known genes in the genome. The ... Shigella are closely related to Escherichia coli, but can be differentiated from E.coli based on pathogenicity, physiology ( ... The genome of pINV is highly conserved between subspecies of S. flexneri. S. flexneri also has two other small multicopy ...
This method was tested by assembling the genome of an ''Escherichia coli'' strain. First, short reads were assembled using the ... De novo genome assembly is used when the genome to be assembled is not similar to any other organisms whose genomes have been ... Genome assembly is normally done by one of two methods: assembly using a reference genome as a scaffold, or de novo assembly. ... Genome assembly presents one of the most challenging tasks in genome sequencing as most modern DNA sequencing technologies can ...
For instance, DUF143 is present in most bacteria and eukaryotic genomes. However, when it was deleted in Escherichia coli no ...
"An expanded genome-scale model of Escherichia coli K-12 (iJR904 GSM/GPR)". Genome Biology. 4 (9): R54. doi:10.1186/gb-2003-4-9- ... Stone SR, Morrison JF (July 1988). "Dihydrofolate reductase from Escherichia coli: the kinetic mechanism with NADPH and reduced ... Helmstaedt K, Krappmann S, Braus GH (September 2001). "Allosteric regulation of catalytic activity: Escherichia coli aspartate ... "Global organization of metabolic fluxes in the bacterium Escherichia coli". Nature. 427 (6977): 839-843. arXiv:q-bio/0403001. ...
"An expanded genome-scale model of Escherichia coli K-12 (iJR904 GSM/GPR)". Genome Biol. 4 (9): R54. doi:10.1186/gb-2003-4-9-r54 ... In eukaryotes the genome is held within the cell nucleus, which is separated from the cytosol by nuclear pores that block the ... Estimates of the number of metabolites in single cells such as E. coli and baker's yeast predict that under 1,000 are made. ... In prokaryotes the cytosol contains the cell's genome, within a structure known as a nucleoid. This is an irregular mass of DNA ...
"An expanded genome-scale model of Escherichia coli K-12 (iJR904 GSM/GPR)". Genome Biology. 4 (9): R54. doi:10.1186/gb-2003-4-9- ... Edwards, J. S.; Ibarra, R. U.; Palsson, B. O. (2001). "In silico predictions of Escherichia coli metabolic capabilities are ... Forster, J.; Famili, I.; Fu, P.; Palsson, B. Ø.; Nielsen, J. (2003). "Genome-Scale Reconstruction of the Saccharomyces ... the development of analysis procedures for genome-scale models, and the experimental verification of these models in ...
"Genome-wide analysis of Fis binding in Escherichia coli indicates a causative role for A-/AT-tracts". Genome Research. 18 (6): ... An occurrence of the cognate sequence motif is about 3000 in the E. coli genome. The estimated abundance of IHF in the growth ... Stonington OG, Pettijohn DE (January 1971). "The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex". ... There are 1464 Fis binding regions distributed across the E. coli genome and a binding motif, identified computationally, ...
The IS128 RNA was initially identified in a computational screen of the E. coli genome. The function of this RNA is unknown. ... The IS128 RNA is a non-coding RNA found in bacteria such as Escherichia coli and Shigella flexneri. The RNA is 209 nucleotides ... "Transcriptome analysis of Escherichia coli using high-density oligonucleotide probe arrays". Nucleic Acids Research. 30 (17): ...
"Clean Genome. E.coli", where 15% of the genome of the parental strain (E. coli K-12 MG1655) were removed to aid in molecular ... "Engineering a reduced Escherichia coli genome". Genome Res. 12 (4): 640-7. doi:10.1101/gr.217202. PMC 187512. PMID 11932248.{{ ... Several differences are present in Mycoplasma mycoides JCVI-syn1.0 relative to the reference genome, notably an E.coli ... While the genome was synthesized chemically in many pieces, it was constructed to match the parent genome closely and ...
"Shotgun Optical Maps of the Whole Escherichia Coli O157:H7 Genome." Genome research 11.9 (2001): 1584-93. Lin, J., et al. " ... Commercial company, Opgen has provided optical mappings for microbial genomes. For larger eukaryotic genomes, only the David C ... Therefore, any sequence reads that are taken from the large template can be mapped onto the genome with a high degree of ... Zhou, S. et al.Validation of rice genome sequence by Optical Mapping. BMC Genomics 8 (2007): 278. Zhou, S. et al. A single ...
1995: The genome of bacterium Haemophilus influenzae is the first genome of a free living organism to be sequenced. 1996: ... 1976: Yeast genes expressed in E. coli for the first time. 1977: DNA is sequenced for the first time by Fred Sanger, Walter ... 2000: The full genome sequence of Drosophila melanogaster is completed. 2001: First draft sequences of the human genome are ... 2003: Successful completion of Human Genome Project with 99% of the genome sequenced to a 99.99% accuracy. 2003: Paul Hebert ...
In Escherichia coli spontaneous zygogenesis (Z-mating) involves cell fusion, and appears to be a form of true sexuality in ... Genome Biol Evol. 10 (11): 3118-3128. doi:10.1093/gbe/evy241. PMC 6263441. PMID 30380054. Gratia JP, Thiry M (September 2003 ... "Spontaneous zygogenesis in Escherichia coli, a form of true sexuality in prokaryotes". Microbiology (Reading, Engl.). 149 (Pt 9 ...