Molecular cloning[edit]. Most strains used by molecular biologists are derivatives of E. coli K-12, and possess both Dam and ... Gardiner-Garden M, Frommer M (July 1987). "CpG islands in vertebrate genomes". Journal of Molecular Biology. 196 (2): 261-82. ... Molecular break light assay for DNA adenine methyltransferase activity - an assay that relies on the specificity of the ... Ying and Li-Byarlay (2015). "Physiological and Molecular Mechanisms of Nutrition in Honey Bees". Advances in Insect Physiology ...
Molecular cloning and expression". Biochem. J. 329 ( Pt 2) (Pt 2): 275-82. PMC 1219041 . PMID 9425109.. ... Molecular function. • zinc ion binding. • peptidase activity. • aminopeptidase activity. • protein binding. • hydrolase ...
"Molecular and Cellular Biology. 25 (14): 5846-58. doi:10.1128/MCB.25.14.5846-5858.2005. PMC 1168807. PMID 15988002.. ... Cloning and Stem Cells. 11 (3): 427-35. doi:10.1089/clo.2009.0024. PMID 19751112.. ... Molecular function. • cytokine activity. • transforming growth factor beta receptor binding. • growth factor activity. • ... "EMBO Molecular Medicine. 9 (4): 531-544. doi:10.15252/emmm.201607231. PMC 5376753. PMID 28270449.. ...
Sambrook, Joseph; Russell, David W. (2001). "Commonly Used Techniques in Molecular Cloning". Molecular Cloning. 3. ... Phenol-chloroform extraction is a liquid-liquid extraction technique in molecular biology used to purify nucleic acids and ...
Molecular Cloning. (4th ed. ed.). Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Pr. ISBN 1936113422. How to extract ... "Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification." Proceedings of the National Academy ... The Hirt extraction process gets rid of the high molecular weight nuclear DNA, leaving only low molecular weight mitochondrial ... Currently it is a routine procedure in molecular biology or forensic analyses. For the chemical method, there are many ...
Molecular cloning and chromosomal localization". Advances in Experimental Medicine and Biology. 414: 253-260. doi:10.1007/978-1 ... Saronwala, A.; Tournay, A.; Gargus, J. J. "Genetic inborn error of metabolism provides a unique window into molecular ... "Two exon-skipping mutations as the molecular basis of succinic semialdehyde dehydrogenase deficiency (4-hydroxybutyric ...
A plasmid is a double stranded circular DNA molecule commonly used for molecular cloning. Plasmids are generally 2 to 4 ... Once a clone from a genomic library is sequenced, the sequence can be used to screen the library for other clones containing ... ISBN 0-8053-9592-X. Russell, David W.; Sambrook, Joseph (2001). Molecular cloning: a laboratory manual. Cold Spring Harbor, N.Y ... This is particularly determined by the number of clones needed to have in a library. The number of clones to get a sampling of ...
Molecular cloning is one of the most commonly used procedures in molecular biology. A gene of interest may be inserted into a ... "Chapter 1". Molecular Cloning - A Laboratory Manual. 1 (3rd ed.). p. 1.27. ISBN 978-0-87969-577-4.. ... "White and green screening with circular polymerase extension cloning for easy and reliable cloning". Protein Science. 22 (6): ... The plasmid carries within the lacZα sequence an internal multiple cloning site (MCS). This MCS within the lacZα sequence can ...
Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. ...
"Future and applications of cloning". Methods Mol. Biol. Methods in Molecular Biology. 348: 319-32. doi:10.1007/978-1-59745-154 ... The attempt to clone a banteng bull was more successful, as were the attempts to clone mouflon (a form of wild sheep), both ... The cell used as the donor for the cloning of Dolly was taken from a mammary gland, and the production of a healthy clone ... Even though Dolly was not the first animal cloned, she received media attention because she was the first cloned from an adult ...
"European Molecular Biology Laboratory - Hamburg. Retrieved 2008-06-07.. *^ Russell DW, Sambrook J (2001). Molecular cloning: a ... and they are a vital tool in molecular cloning.[8][9][10] ... Enzymes of Molecular Biology. Methods of Molecular Biology. 16 ... "Microbiology and Molecular Biology Reviews. 64 (2): 412-34. doi:10.1128/MMBR.64.2.412-434.2000. PMC 98998. PMID 10839821.. ... this characteristic is widely used to perform in-vitro cloning techniques such as Golden Gate cloning. These enzymes may ...
Tomato as a model system: I. Genetic and physical mapping of jointless". MGG Molecular & General Genetics. 242 (6). doi:10.1007 ... Tomatoes have been used as a model in map-based cloning, where transgenic plants must be created to prove that a gene has been ... Foolad, M. R. (2007). "Current Status Of Breeding Tomatoes For Salt And Drought Tolerance". Advances in Molecular Breeding ... Wing, R.; Zhang, H. B.; Tanksley, S. (1994). "Map-based cloning in crop plants. ...
Molecular cloning and characterization". J Biol Chem. 277 (45): 43474-80. doi:10.1074/jbc.M206065200. PMID 12213818. "Entrez ...
Molecular cloning and sequence". The Journal of Biological Chemistry. 265 (27): 16060-3. PMID 2168878. Vandewalle J, Bauters M ... It is a mode of cell death defined by characteristic morphological, biochemical and molecular changes. It was first described ... Molecular aspects of medicine. 34 (2-3): 485-93. doi:10.1016/j.mam.2012.05.006. PMID 23506884. Check date values in: ,date= ( ... Somatic Cell and Molecular Genetics. 16 (2): 143-9. doi:10.1007/BF01233044. PMID 2157297. Schlattner U, Dolder M, Wallimann T, ...
20.109(S07): Start-up Genome Engineering [1] Phage Display: A Laboratory Manual Messing, J (1993). "M13 cloning vehicles. Their ... Journal of Molecular Biology. 228 (3): 720-4. doi:10.1016/0022-2836(92)90858-h. PMID 1469710. Huang, Yu; Chung-Yi Chiang; Soo ... Methods in Molecular Biology. 23. pp. 9-22. doi:10.1385/0-89603-248-5:9. ISBN 0-89603-248-5. PMID 8220775. Archived from the ...
Molecular cloning and expression". Eur. J. Biochem. 191 (3): 619-625. doi:10.1111/j.1432-1033.1990.tb19166.x. PMID 2390989. ... by isolation of genomic clones and complementary DNA clones utilizing polymerase chain reaction". J. Biol. Chem. 265 (2): 1102- ... 1992). "Molecular analysis of human glycophorin MiIX gene shows a silent segment transfer and untemplated mutation resulting ...
Ohara, O. (2009). "ORFeome Cloning". Reverse Chemical Genetics. Methods in Molecular Biology. 577. pp. 3-9. doi:10.1007/978-1- ... In, molecular genetics, an ORFeome refers to the complete set of open reading frames (ORFs) in a genome. The term may also be ... Complete ORF sets have been cloned for a number of organisms including Brucella melitensis Chlamydia pneumoniae Escherichia ... a resource for comparative molecular microbiology". BMC Genomics. 11: 470. doi:10.1186/1471-2164-11-470. PMC 3091666 . PMID ...
Kitagawa H, Izumikawa T, Uyama T, Sugahara K (2003). "Molecular cloning of a chondroitin polymerizing factor that cooperates ... 2003). "Chondroitin sulfate synthase-3. Molecular cloning and characterization". J. Biol. Chem. 278 (41): 39711-25. doi:10.1074 ... "Molecular cloning and expression of a human chondroitin synthase". J Biol Chem. 276 (42): 38721-6. doi:10.1074/jbc.M106871200. ... The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 6 (1): 63-70. doi: ...
Molecular cloning and expression". Biochem. J. 329 ( Pt 2) (Pt 2): 275-82. PMC 1219041 . PMID 9425109. Simaga S, Babić D, Osmak ...
Russell MW, Kemp P, Wang L, Brody LC, Izumo S (December 1998). "Molecular cloning of the human HAND2 gene". Biochimica et ... Molecular function. • DNA binding. • sequence-specific DNA binding. • RNA polymerase II regulatory region sequence-specific DNA ... Srivastava D, Gottlieb PD, Olson EN (2003). "Molecular mechanisms of ventricular hypoplasia". Cold Spring Harbor Symposia on ... Srivastava D (1999). "HAND proteins: molecular mediators of cardiac development and congenital heart disease". Trends in ...
"Molecular cloning of the human eosinophil peroxidase. Evidence for the existence of a peroxidase multigene family". J. Exp. Med ... Petrides, PE (September 1998). "Molecular genetics of peroxidase deficiency". Journal of molecular medicine (Berlin, Germany). ... Molecular weight: 57 kDa (heavy chain), 11 kDa (light chain) (predicted); 52 kDa, 15 kDa (observed) Isoelectric point pI = ... Lanza, F (September 1998). "Clinical manifestation of myeloperoxidase deficiency". Journal of molecular medicine (Berlin, ...
European Molecular Biology Laboratory - Hamburg. Retrieved 2008-06-07. Russell DW, Sambrook J (2001). Molecular cloning: a ... These enzymes are routinely used for DNA modification in laboratories, and they are a vital tool in molecular cloning. The term ... Clark DP (2005). Molecular biology. Amsterdam: Elsevier Academic Press. ISBN 0-12-175551-7. Goodsell DS (2002). "The molecular ... this characteristic is widely used to perform in-vitro cloning techniques such as Golden Gate cloning. These enzymes may ...
Iwahana H, Oka J, Mizusawa N, Kudo E, Ii S, Yoshimoto K, Holmes EW, Itakura M (Jan 1993). "Molecular cloning of human ... Molecular and Cellular Biology portal This article incorporates text from the United States National Library of Medicine, which ... Zalkin H, Dixon JE (1992). "De novo purine nucleotide biosynthesis". Progress in Nucleic Acid Research and Molecular Biology. ...
Purification, molecular cloning, and characterization". J. Biol. Chem. 279: 38555-38562. doi:10.1074/jbc.m406886200. PMID ... Mannosylglycoprotein endo-beta-mannosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and ...
2012) "Labeling of DNA Probes by Nick Translation". Molecular Cloning: A Laboratory Manual. Park, K; Kim, J; Lim, S; Han, S; ... Methods in molecular biology (Clifton, N.J.). Methods in Molecular Biology. 659. pp. 3-20. doi:10.1007/978-1-60761-789-1_1. ... Methods in molecular biology (Clifton, N.J.). Methods in Molecular Biology. 659. pp. 51-70. doi:10.1007/978-1-60761-789-1_4. ... Next, the clones are sequenced and their position on the genome is verified. Probe labelling can be carried out by using either ...
The choice of vector for molecular cloning depends on the choice of host organism, the size of the DNA to be cloned, and ... One is that molecular cloning involves replication of the DNA within a living cell, while PCR replicates DNA in the test tube, ... Molecular cloning is the laboratory process used to create recombinant DNA. It is one of two most widely used methods, along ... These steps are described in some detail in a related article (molecular cloning). Following transplantation into the host ...
Neuberger, M. S.; Honjo, T.; Alt, Frederick W. (2004). Molecular biology of B cells. Amsterdam: Elsevier. pp. 189-191. ISBN 0- ... the activation and growth of B cell clones able to secrete antibodies of higher affinity for the antigen. ...
MOLECULAR CLONING AND FUNCTIONAL CHARACTERIZATION". Journal of Biological Chemistry. 277 (26): 23294-23300. doi:10.1074/jbc. ... Molecular Cell. 13 (5): 677-688. doi:10.1016/S1097-2765(04)00083-8. PMID 15023338. Sugiura, M.; Kono, K.; Liu, H.; Shimizugawa ...