Loading...
*  Molecular cloning
Obtaining the molecular clone of a gene can lead to the development of organisms that produce the protein product of the cloned ... In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated ... Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and ... In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host ...
*  Subcloning
Cloning Molecular cloning Polymerase chain reaction TA cloning Williams, Steven A.; et al. (2006). Laboratory Investigations in ... Subcloning is not to be confused with molecular cloning, a related technique. Restriction enzymes are used to excise the gene ... In molecular biology, subcloning is a technique used to move a particular DNA sequence from a parent vector to a destination ... Molecular Biology. p. 213. CS1 maint: Explicit use of et al. (link). ...
*  Phenol-chloroform extraction
Sambrook, Joseph; Russell, David W. (2001). "Commonly Used Techniques in Molecular Cloning". Molecular Cloning. 3. ... Phenol-chloroform extraction is a liquid-liquid extraction technique in molecular biology used to purify nucleic acids and ...
*  DNA extraction
Molecular Cloning. (4th ed. ed.). Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Pr. ISBN 1936113422. How to extract ... "Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification." Proceedings of the National Academy ... The Hirt extraction process gets rid of the high molecular weight nuclear DNA, leaving only low molecular weight mitochondrial ... Currently it is a routine procedure in molecular biology or forensic analyses. For the chemical method, there are many ...
*  HIST1H2BA
Molecular cloning and characterization". J Biol Chem. 277 (45): 43474-80. doi:10.1074/jbc.M206065200. PMID 12213818. "Entrez ...
*  SLC25A5
Molecular cloning and sequence". The Journal of Biological Chemistry. 265 (27): 16060-3. PMID 2168878. Vandewalle J, Bauters M ... It is a mode of cell death defined by characteristic morphological, biochemical and molecular changes. It was first described ... Molecular aspects of medicine. 34 (2-3): 485-93. doi:10.1016/j.mam.2012.05.006. PMID 23506884. Check date values in: ,date= ( ... Somatic Cell and Molecular Genetics. 16 (2): 143-9. doi:10.1007/BF01233044. PMID 2157297. Schlattner U, Dolder M, Wallimann T, ...
*  GYPE
Molecular cloning and expression". Eur. J. Biochem. 191 (3): 619-625. doi:10.1111/j.1432-1033.1990.tb19166.x. PMID 2390989. ... by isolation of genomic clones and complementary DNA clones utilizing polymerase chain reaction". J. Biol. Chem. 265 (2): 1102- ... 1992). "Molecular analysis of human glycophorin MiIX gene shows a silent segment transfer and untemplated mutation resulting ...
*  CHSY1
Kitagawa H, Izumikawa T, Uyama T, Sugahara K (2003). "Molecular cloning of a chondroitin polymerizing factor that cooperates ... 2003). "Chondroitin sulfate synthase-3. Molecular cloning and characterization". J. Biol. Chem. 278 (41): 39711-25. doi:10.1074 ... "Molecular cloning and expression of a human chondroitin synthase". J Biol Chem. 276 (42): 38721-6. doi:10.1074/jbc.M106871200. ... The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 6 (1): 63-70. doi: ...
*  DPP3
Molecular cloning and expression". Biochem. J. 329 ( Pt 2) (Pt 2): 275-82. PMC 1219041 . PMID 9425109. Simaga S, Babić D, Osmak ...
*  Restriction enzyme
European Molecular Biology Laboratory - Hamburg. Retrieved 2008-06-07. Russell DW, Sambrook J (2001). Molecular cloning: a ... These enzymes are routinely used for DNA modification in laboratories, and they are a vital tool in molecular cloning. The term ... Clark DP (2005). Molecular biology. Amsterdam: Elsevier Academic Press. ISBN 0-12-175551-7. Goodsell DS (2002). "The molecular ... this characteristic is widely used to perform in-vitro cloning techniques such as Golden Gate cloning. These enzymes may ...
*  Amidophosphoribosyltransferase
Iwahana H, Oka J, Mizusawa N, Kudo E, Ii S, Yoshimoto K, Holmes EW, Itakura M (Jan 1993). "Molecular cloning of human ... Molecular and Cellular Biology portal This article incorporates text from the United States National Library of Medicine, which ... Zalkin H, Dixon JE (1992). "De novo purine nucleotide biosynthesis". Progress in Nucleic Acid Research and Molecular Biology. ...
*  Genomic library
A plasmid is a double stranded circular DNA molecule commonly used for molecular cloning. Plasmids are generally 2 to 4 ... Once a clone from a genomic library is sequenced, the sequence can be used to screen the library for other clones containing ... ISBN 0-8053-9592-X. Russell, David W.; Sambrook, Joseph (2001). Molecular cloning: a laboratory manual. Cold Spring Harbor, N.Y ... This is particularly determined by the number of clones needed to have in a library. The number of clones to get a sampling of ...
*  Mannosylglycoprotein endo-beta-mannosidase
Purification, molecular cloning, and characterization". J. Biol. Chem. 279: 38555-38562. doi:10.1074/jbc.m406886200. PMID ... Mannosylglycoprotein endo-beta-mannosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and ...
*  Chromogenic in situ hybridization
2012) "Labeling of DNA Probes by Nick Translation". Molecular Cloning: A Laboratory Manual. Park, K; Kim, J; Lim, S; Han, S; ... Methods in molecular biology (Clifton, N.J.). Methods in Molecular Biology. 659. pp. 3-20. doi:10.1007/978-1-60761-789-1_1. ... Methods in molecular biology (Clifton, N.J.). Methods in Molecular Biology. 659. pp. 51-70. doi:10.1007/978-1-60761-789-1_4. ... Next, the clones are sequenced and their position on the genome is verified. Probe labelling can be carried out by using either ...
*  Recombinant DNA
The choice of vector for molecular cloning depends on the choice of host organism, the size of the DNA to be cloned, and ... One is that molecular cloning involves replication of the DNA within a living cell, while PCR replicates DNA in the test tube, ... Molecular cloning is the laboratory process used to create recombinant DNA. It is one of two most widely used methods, along ... These steps are described in some detail in a related article (molecular cloning). Following transplantation into the host ...
*  Pleckstrin homology domain
MOLECULAR CLONING AND FUNCTIONAL CHARACTERIZATION". Journal of Biological Chemistry. 277 (26): 23294-23300. doi:10.1074/jbc. ... Molecular Cell. 13 (5): 677-688. doi:10.1016/S1097-2765(04)00083-8. PMID 15023338. Sugiura, M.; Kono, K.; Liu, H.; Shimizugawa ...
*  Blue-white screen
Molecular cloning is one of the most commonly used procedures in molecular biology. A gene of interest may be inserted into a ... "Chapter 1". Molecular Cloning - A Laboratory Manual. 1 (3rd ed.). p. 1.27. ISBN 978-0-87969-577-4. J., Ninfa, Alexander (1998 ... "White and green screening with circular polymerase extension cloning for easy and reliable cloning". Protein Science. 22 (6): ... The recombinant clones can be further analyzed by isolating and purifying small amounts of plasmid DNA from the transformed ...
*  Succinic semialdehyde dehydrogenase deficiency
Molecular cloning and chromosomal localization". Advances in Experimental Medicine and Biology. 414: 253-260. doi:10.1007/978-1 ... Saronwala, A.; Tournay, A.; Gargus, J. J. "Genetic inborn error of metabolism provides a unique window into molecular ... "Two exon-skipping mutations as the molecular basis of succinic semialdehyde dehydrogenase deficiency (4-hydroxybutyric aciduria ...
*  D-threonine aldolase
Molecular cloning and cofactor characterization". J. Biol. Chem. 273: 16678-16685. doi:10.1074/jbc.273.27.16678. PMID 9642221. ... Liu, J.Q.; Odani, M.; Yasuoka, T.; Dairi, T.; Itoh, N.; Kataoka, M.; Shimizu, S.; Yamada, H. (2000). "Gene cloning and ... D-threonine aldolase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular Biology ...
*  Isoamyl alcohol
"Purification of Nucleic Acids: Extraction with Phenol-Chloroform". Molecular Cloning: A Laboratory Manual. Cold Spring Harbor ...
*  COASY
"Molecular cloning of CoA Synthase. The missing link in CoA biosynthesis". J Biol Chem. 277 (25): 22107-10. doi:10.1074/jbc. ...
*  Gel electrophoresis
Sambrook J, Russel DW (2001). Molecular Cloning: A Laboratory Manual 3rd Ed. Cold Spring Harbor Laboratory Press. Cold Spring ... "Chapter 5, protocol 1". Molecular Cloning - A Laboratory Manual. 1 (3rd ed.). p. 5.2-5.3. ISBN 978-0879691363. Smisek, D. L.; ... There are molecular weight size markers available that contain a mixture of molecules of known sizes. If such a marker was run ... There are also limitations in determining the molecular weight by SDS-PAGE, especially if you are trying to find the MW of an ...
*  Ceramide kinase
Molecular cloning and functional characterization". J. Biol. Chem. 277 (26): 23294-300. doi:10.1074/jbc.M201535200. PMID ... A mouse homolog has been cloned as well. The complete gene of human CERK contains 4459bp, which consists of a 123bp-5'- ...
*  Agarose
"Chapter 5, protocol 1". Molecular Cloning - A Laboratory Manual. 1 (3rd ed.). p. 5.7. ISBN 978-0-87969-577-4. Keren, David (26 ... "Chapter 5, protocol 6". Molecular Cloning - A Laboratory Manual. 1. p. 5.29. ISBN 978-0879695774. Lee PY, Costumbrado J, Hsu CY ... "Chapter 5, protocol 1". Molecular Cloning - A Laboratory Manual. 1. p. 5.4. ISBN 978-0879691363. Alistair M. Stephen; Glyn O. ... "Chapter 5, protocol 1". Molecular Cloning - A Laboratory Manual. 1. p. 5.2-5.3. ISBN 978-0879691363. David Freifelder (1982). ...
*  Gel electrophoresis of nucleic acids
"Chapter 5, protocol 1". Molecular Cloning - A Laboratory Manual. 1 (3rd ed.). p. 5.2. ISBN 978-0-87969-577-4. Aaij C, Borst P ( ... "Chapter 5, protocol 1". Molecular Cloning - A Laboratory Manual. 1 (3rd ed.). pp. 5.5-5.6. ISBN 978-0-87969-577-4. Blasiak J, ... G. Lucotte; F. Baneyx (1993). Introduction to Molecular Cloning Techniques. Wiley-Blackwell. p. 32. ISBN 978-0471188490. Joseph ... However, the migration of DNA molecules in solution, in the absence of a gel matrix, is independent of molecular weight during ...
*  Phage display
Multiple cloning sites are sometimes used to ensure that the fragments are inserted in all three possible reading frames so ... This technology was further developed and improved by groups at the Laboratory of Molecular Biology with Greg Winter and John ... Initial work was done by laboratories at the MRC Laboratory of Molecular Biology (Greg Winter and John McCafferty), the Scripps ... Danner S, Belasco JG (November 2001). "T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from ...