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*  Bacterial artificial chromosome
These constructs are referred to as "infectious clones", as transfection of the BAC construct into host cells is sufficient to ... A similar cloning vector called a PAC has also been produced from the DNA of P1 bacteriophage. BACs are often used to sequence ... parA and parB for partitioning F plasmid DNA to daughter cells during division and ensures stable maintenance of the BAC. A ... Shizuya H, Birren B, Kim UJ, Mancino V, Slepak T, Tachiiri Y, Simon M (1992). "Cloning and stable maintenance of 300-kilobase- ...
*  Molecular cloning
... cDNA cloning is usually used to obtain clones representative of the mRNA population of the cells of interest, while synthetic ... free of living cells. In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven ... The methods used to get DNA into cells are varied, and the name applied to this step in the molecular cloning process will ... This complex mixture is sorted out in subsequent steps of the cloning process, after the DNA mixture is introduced into cells. ...
*  Clone (cell biology)
Clone (B-cell biology) Cloning List of animals that have been cloned Polyclonal antibodies Polyclonal response "Clone ... so they are technically a single clone of cells. However, during course of cell division, one of the cells can get mutated ... bone marrow cells. Most other cells cannot divide indefinitely as after a few cycles of cell division the cells stop expressing ... A clone is a group of identical cells that share a common ancestry, meaning they are derived from the same cell. Clonality ...
*  Clone (B-cell biology)
Clone (cell biology) Nossal, G. J. V. & Lederberg, J. (1958). "Antibody production by single cells". Nature. 181: 1419-1420. ... The expansion of a particular clone of immune B cells is usually interpreted by clinicians as evidence of unrestricted cell ... This differentiation and activation of the B cell occurs most rapidly after exposure to antigen by antigen-presenting cells in ... The process of immunological B-cell maturation involves transformation from an undifferentiated B cell to one that secretes ...
*  2014 in science
Alice Park (April 17, 2014). "Researchers Clone Cells From Two Adult Men". TIME. Retrieved April 17, 2014. "Leukaemia: Cardiff ... fat cells into brown, or "good," fat cells. Two compounds have already been shown to achieve this in human cells. 10 December ... "Stem cells created from a drop of blood: DIY finger-prick technique opens door for extensive stem cell banking". Science Daily ... "Stap cells: research paper on stem cell breakthrough was partly falsified". The Guardian. 1 April 2014. Retrieved 1 April 2014 ...
*  Human cloning
... therapeutic cloning and reproductive cloning. Therapeutic cloning would involve cloning cells from a human for use in medicine ... 24 January 2018). "Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer". Cell (journal). doi:10.1016/j.cell.2018.01.020 ... Trounson A, DeWitt ND (2013). "Pluripotent stem cells from cloned human embryos: success at long last". Cell Stem Cell. 12 (6 ... "Human somatic cell nuclear transfer using adult cells". Cell Stem Cell. 14 (6): 777-80. doi:10.1016/j.stem.2014.03.015. PMID ...
*  Xenbase
This process (transfer of nuclei from cloned cells) is referred to as serial transplantation. To provide examples of how ... a liver cell nucleus remains a liver cell nucleus and cannot return to an undifferentiated state). Specifically, John Gurdon's ... Genome browser BLAST Expression Search and Clone Search (search by gene symbol, gene name, or Affymetrix id) Gene nomenclature ... Importance: Gurdon's experiments challenged the dogma of the time which suggested that the nucleus of a differentiated cell is ...
*  Katherine Sanford
Additionally, cloning allowed the development of new vaccines and advanced the study of stem cells. Later in her career Sanford ... Virginia Evans and a group of tissue-culture workers she became the first to successfully clone a mammalian cell in vitro. This ... In the 1940s she became the first person to successfully clone a mammalian cell in vitro, which allowed for more efficient ... The cell cultures were exposed to fluorescent light that damaged the DNA of the cells. Following this, they were treated with ...
*  TAL1
J. Cell Cloning. 10 (5): 269-76. doi:10.1002/stem.5530100504. PMID 1453013. Aplan PD, Lombardi DP, Reaman GH, Sather HN, ... T-cell acute lymphocytic leukemia protein 1 is a protein that in humans is encoded by the TAL1 gene. The protein encoded by ... Cell. Biol. 10 (12): 6426-35. PMC 362919 . PMID 2247063. Chen Q, Cheng JT, Tasi LH, Schneider N, Buchanan G, Carroll A, Crist W ... Cell. Biol. 20 (6): 2248-59. doi:10.1128/mcb.20.6.2248-2259.2000. PMC 110841 . PMID 10688671. Lécuyer E, Herblot S, Saint-Denis ...
*  Kevin Andrews (politician)
"Stem cell cloning ban overturned". News.com.au. 6 December 2006. Retrieved 31 August 2007. [dead link] "Andrews sets out ... In taking a stance against stem cell research in 2002, he stated that it was the "first time" that "human beings can be treated ... "Politicians prepare to vote on stem cell issue". Australian Broadcasting Corporation. 15 August 2002. Retrieved 31 August 2007 ... euthanasia or embryonic stem cell experimentation. Andrews has described his role with LDI as an "honorary patronage". In 2007 ...
*  Gábor Vajta
"Somatic Cell Cloning without Micromanipulators". Cloning. 3 (2): 89-95. doi:10.1089/15204550152475590. PMID 11900643. Vajta, G ... "Birth of a cloned calf derived from a vitrified hand-made cloned embryo". Reproduction, Fertility and Development. 15 (7): 361 ... OPS is also regarded as the most appropriate carrier tool for vitrification of human embryonic stem cells (hESCs). Vajta, G. B ... All work is done by hand, with simple handheld tools (blade and micropipette).The first cloned animals in Africa and ...
*  Prowler (comics)
Julia arrives and fends Electro off as she tells Hobie that Spider-Man stabilized the human and clone cells. When Electro gets ... The clone assists Jean DeWolff's clone in helping Spider-Man get away from the villains. Kaine Parker later told Spider-Man ... 2 #3 The Clone Conspiracy #3 Prowler vol. 2 #4 The Clone Conspiracy #4 House of M: Avengers #1-5 Ultimate Fantastic Four #25 ... It is revealed that this Prowler is a clone that Jackal had gathered to grow clones with false memories that span all the way ...
*  C. Garrison Fathman
Clones of alloreactive T cells. Nature 272:617-8. Kimoto, Masao, and Fathman, C. Garrison. (1981) Antigen-reactive T cell ... His research centers on a molecular and cellular understanding of CD4 T cell responsiveness in mice and man. President of the ... 1996) Monoclonal T cells identified in early NOD islet infiltrates. Immunity 4:189-194. Anandasabapathy, Niroshana, Ford, ... 2004) Two isoforms of otubain 1 regulate T cell anergy via GRAIL. Nat Immunol 5:45-54. Kodama, Keiichi, Butte, Atul J., Creusot ...
*  North East England
"Green light for stem cell clones". The Guardian. Retrieved 22 August 2013. Pearson, Adrian. "Nissan plans its expansion of ... Newcastle University was the first in the UK and the second in Europe to receive a licence to perform research on stem cells ... Newcastle University is now a leading institution in the development of stem cell technology being the first in the United ... Dr Karim Nayernia was the first to isolate spermatagonial stem cells at this University. Many new healthcare developments have ...
*  Boston Children's Hospital
Evan Snyder clones the first neural stem cells from the human central nervous system of a fetus,, offering the possibility of ... "Nerve Cell Clones Repair Brain Damage". The Harvard University Gazette. Archived from the original on 3 October 1999. Retrieved ... even in the absence of conventional T-helper cells. Moreover, NKT cells respond to a different class of antigens than are ... To date, cell lines have been distributed to over 65 laboratories worldwide.[citation needed] In 2010, a drug that boosts ...
*  Prototype pattern
When a cell splits, two cells of identical genotype result. In other words, the cell clones itself. The Prototype design ... method clone() is output: a WordOccurrences object containing the same data. Call clone() on the super class. On the returned ... The mitotic division of a cell - resulting in two identical cells - is an example of a prototype that plays an active role in ... You can see here, we have used Clone method to clone the prototype when required. // Prototype pattern public abstract class ...
*  Michael L. Brodman
"Amniotic Fluid Cells Are More Efficiently Reprogrammed to Pluripotency Than Adult Cells". Cloning Stem Cells. 12: 117-25. doi: ... 10.1089/cell.2009.0077. PMC 2998987 . PMID 20677926. Copperman, KB; Schertz, JC; Witkin, G; Sandler, B; Brodman, M; Copperman, ...
*  Glucocorticoid
... s cause B cells to express smaller amounts of IL-2 and of IL-2 receptors. This diminishes both B cell clone ... The effect is more prominent in immature T cells still inside in the thymus, but peripheral T cells are also affected. The ... including both B cells and T cells. The major mechanism for this immunosuppression through inhibition of nuclear factor kappa- ... including both B cells and T cells), monocytes, macrophages, and the anatomical barrier function of the skin. This suppression ...
*  Immunosuppressive drug
This diminishes both B cell clone expansion and antibody synthesis. Glucocorticoids influence all types of inflammatory events ... In this way, it prevents the cell from transitioning from the G0 into G1 phase of the cell cycle. Tacrolimus is more potent ... This lowers the number of available T-cells, perhaps by sensitizing them for the uptake by the epithelial reticular cells. The ... They affect the proliferation of both T cells and B cells. Due to their highest effectiveness, purine analogs are most ...
*  Tong Dizhou
Some of the clone cells grew into an embryo and developed into juvenile goldfish, the first artificially cloned fish in the ... Cellular cloning refers to producing genetically identical organisms by transferring the nucleus of a donor cell into a host ... The researchers found that some of the resultant clone cells in the juvenile fish had traits previously found just in Rhodeus ... Tong demonstrated that in hairy sea squirts, during the third division of cells in an embryo, cells divide unequally. Tong ...
*  Hwang Woo-suk
Hwang claimed he and his team had extracted stem cells from cloned human embryos. However, further examination of the cells' ... Hwang maintained that two of his 11 forged stem cell lines had been maliciously switched for cells from regular, not cloned, ... 100,000 each to have their dogs cloned. Hwang's intention to develop better technique for cloning was focused on stem cells ... each new cell has the potential to either remain a stem cell or become another type of cell with a more specialized function. ...
*  HEK 293 cells
... obtaining just one clone of cells that were cultured for several months. After presumably adapting to tissue culture, cells ... 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown ... As a consequence, HEK 293 cells should not be used as an in vitro model of typical kidney cells. HEK 293 cells have a complex ... HEK 293 Transfection and Selection Data @ Cell-culture Database A HEK293 Cell Database 293 Cells (CRL-1573) in the ATCC ...
*  Cloning
... in biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), ... Cloning a cell means to derive a population of cells from a single cell. In the case of unicellular organisms such as bacteria ... 24 January 2018). "Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer". Cell (journal). doi:10.1016/j.cell.2018.01.020 ... However, in the case of cell cultures from multi-cellular organisms, cell cloning is an arduous task as these cells will not ...
*  Ann Kiessling
Cook, G. (2001, Jul 13). WORCESTER FIRM AIMS TO CLONE HUMAN CELLS. Boston Globe. Krasner, J. (2003, Apr 09). KEEPING ACT ... 2001, Jul 13). Scientists pursue controversial goal: Cloning for tissue --- resulting cells might cure life-threatening ... Ann Kiessling Devotes Full Time to Bedford Stem Cell Research Foundation". Bedford Stem Cell Research Foundation. Retrieved 23 ... "About the director". Bedford Stem Cell Research Foundation. Retrieved 8 November 2012. "Bedford Stem Cell Research Foundation ...
*  Interleukin 3
... identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3". Cell. 47 (1): 3-10. doi: ... He found a T cell derived factor that induced the synthesis of 20alpha-hydroxysteroid dehydrogenase in hematopoietic cells and ... In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), ... Interleukin 3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the ...
*  Phage display
Multiple cloning sites are sometimes used to ensure that the fragments are inserted in all three possible reading frames so ... The use of a helper phage can be eliminated by using 'bacterial packaging cell line' technology. Elution can be done combining ... Then the expression of single chain Fv's (scFv), and single chain T cell receptors (scTCR) were expressed both with and without ... Endemann H, Model P (July 1995). "Location of filamentous phage minor coat proteins in phage and in infected cells". J. Mol. ...
*  Monoclonal B-cell lymphocytosis
February 2009). "B-Cell Clones as Early Markers for Chronic Lymphocytic Leukemia". N Engl J Med. 360 (7): 659-667. doi:10.1056/ ... Molecular techniques can detect monoclonal B cell levels as low as 3-5 B cells/microliter (comparable to the amount of stem ... "monoclonal B-cell lymphocytosis" was proposed by a consensus committee in 2005 to indicate a monoclonal B cell population in a ... Monoclonal B-cell lymphocytosis (MBL) is a condition that resembles chronic lymphocytic leukemia (CLL), but does not meet the ...