The iCapTag™-target protein complex can be expressed in a wide range of expression hosts (e.g. CHO and E.coli cells). It is not ... Zhang, Jin; Campbell, Robert; Ting, Alice; Tsien, Roger (2002). "Creating new fluorescent probes for cell biology". Nat Rev Mol ... Zhang, Jin; Campbell, Robert; Ting, Alice; Tsien, Roger (2002). "Creating new fluorescent probes for cell biology". Nat Rev Mol ... Cell Chemical Biology. 29 (2): 339-350.e10. doi:10.1016/j.chembiol.2021.07.005. ISSN 2451-9456. PMC 8878318. PMID 34324879. Tan ...
It is produced in CHO cells. In September 2020, phase I clinical trials of UB-612 started in Taiwan. and in January 2021, phase ... May 2022). "A multitope SARS-CoV-2 vaccine provides long-lasting B cell and T cell immunity against Delta and Omicron variants ... UB-612 provides B-cell and T-cell memory immunity and offers a potential as a universal vaccine to fend off the Omicron variant ... It is composed of SARS-CoV-2 S1-RBD protein and synthetic peptides representing T cell (Th and CTL) epitopes on the ...
Efti is manufactured in CHO cells. Immutep worked with Henogen as the contracted manufacturing organization to provide efti for ... Activated T cells. The eight patients experienced sustained CD8+ T-cell activation (as measured by percentage of CD8+ T cells ... Increase in relevant cell numbers. There was a sustained increase in the number of monocytes, NK cells, and activated CD8+ T ... Cell Genesys' lead product, GVAX, consisted of whole tumor cells genetically modified to secrete GM-CSF. When mice that had ...
... it is manufactured in CHO cells. As of 2016, it was in phase III studies for induction and maintenance therapy in people with ... Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, ... "Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, ...
It is manufactured recombinantly in CHO cells. The monoclonal antibody 2C4 appears to have first been published in 1990 by ... sets off signal transduction through several pathways that stimulate cell proliferation and cell growth; if overexpressed it ... Badache A, Hynes NE (April 2004). "A new therapeutic antibody masks ErbB2 to its partners". Cancer Cell. 5 (4): 299-301. doi: ... In both uses, more than 10% of people additionally experienced: loss of red blood cells, hypersensitivity or allergic reaction ...
Cho repeatedly placed harassing cell phone calls to Koch as "Cho's brother, 'Question Mark'," a name Cho also used when ... She said that Cho resisted speaking in class and took cell phone pictures of her. After Roy became concerned with Cho's ... Cho's grandfather reported his concerns about Cho's behavior during childhood. According to Cho's grandfather, Cho never made ... Cho was sent to a psychiatrist. Cho graduated from Westfield High School in 2003. Cho was diagnosed with selective mutism. The ...
It is recombinantly manufactured in Chinese hamster ovary (CHO) cells. Pembrolizumab was invented by scientists at Organon ... Normally, the PD-1 receptor on activated T-cells binds to the PD-L1 or PD-L2 ligands present on normal cells in the body, ... deactivating any potential cell-mediated immune response against these cells. Many cancers make proteins such as PD-L1 that ... Results of a Phase II clinical trial in Merkel-cell carcinoma were reported in The New England Journal of Medicine in June 2016 ...
"Phase I Trial of a Recombinant COVID-19 Vaccine (CHO Cell)". ClinicalTrials.gov. United States National Library of Medicine. 19 ... "Efficacy, safety and immunogenicity of a recombinant protein subunit vaccine (CHO cells) against COVID-19 in adults: an ... "Phase II Clinical Trial of Recombinant SARS-CoV-2 Spike Protein Vaccine (CHO Cell)". ClinicalTrials.gov. 4 August 2021. ... "Phase I Clinical Trial of Recombinant SARS-CoV-2 Spike Protein Vaccine (CHO Cell)". ClinicalTrials.gov. 4 July 2021. ...
The FDA has approved ERTs that are derived from other human cells, animal cells (i.e. Chinese hamster ovary cells, or CHO cells ... These cells have the ability to mature into the many cell types that comprise blood, including red blood cells, platelets, and ... Hematopoietic stem cell (HSC) transplantation is another treatment for lysosomal storage diseases. HSCs are derived from bone- ... Once inside the body the vector introduces the therapeutic gene into host cells, and the protein encoded by the newly inserted ...
"Phleomycin resistance as a dominant selectable marker in CHO cells". Somatic Cell and Molecular Genetics. 14 (3): 243-52. doi: ... and animal cells. It causes cell death by intercalating into DNA and inducing double stranded breaks of the DNA. Zeocin is a ... When Zeocin enters a cell, the Cu2+ is reduced to Cu+ and then removed. Subsequently, Zeocin becomes activated and can then ... especially for the selection of eukaryotic cell lines when used in conjunction with vectors containing a selectable marker for ...
Thielemans L, Depoortere I, Vanden Broeck J, Peeters TL (May 2002). "The motilin pharmacophore in CHO cells expressing the ... It is produced by enteroendocrine cells in the proximal small intestine and secreted cyclically. Motilin mimetics could be used ... "Demonstration of a functional motilin receptor in TE671 cells from human cerebellum". Brain Research. 895 (1-2): 119-128. doi: ...
CHO) cells were isolated, and this gene restored UV resistance to cells of complementation group 1. The human ERCC1 gene ... Wood RD, Burki HJ, Hughes M, Poley A (February 1983). "Radiation-induced lethality and mutation in a repair-deficient CHO cell ... Hayashi T, Takao M, Tanaka K, Yasui A (June 1998). "ERCC1 mutations in UV-sensitive Chinese hamster ovary (CHO) cell lines". ... Mammalian cells with mutant ERCC1-XPF are moderately more sensitive than normal cells to agents (such as ionizing radiation) ...
Hayashi T, Takao M, Tanaka K, Yasui A (Jun 1998). "ERCC1 mutations in UV-sensitive Chinese hamster ovary (CHO) cell lines". ... DNA repair protein complementing XP-A cells is a protein that in humans is encoded by the XPA gene. Nucleotide excision repair ... "Analysis of 133 meioses places the genes for nevoid basal cell carcinoma (Gorlin) syndrome and Fanconi anemia group C in a 2.6- ...
Yokomizo T, Noiri E, Izumi T, Shimizu T (2003). "In vivo chemotaxis using CHO cells expressing human leukotriene B4 receptor". ... Kato K, Yokomizo T, Izumi T, Shimizu T (2000). "Cell-specific transcriptional regulation of human leukotriene B(4) receptor ... "Molecular cloning of a novel P2 purinoceptor from human erythroleukemia cells". J Biol Chem. 271 (31): 18363-7. doi:10.1074/jbc ... "Leukotriene B4 receptor antagonist LY293111 inhibits proliferation and induces apoptosis in human pancreatic cancer cells". ...
Wood RD, Burki HJ, Hughes M, Poley A (Feb 1983). "Radiation-induced lethality and mutation in a repair-deficient CHO cell line ... If the stem cells at the base of the crypt express ERCC4 (XPF), generally all several thousand cells of the crypt will also ... Multiple independent complementation groups of Chinese hamster ovary (CHO) cells have been isolated, and this gene restored UV ... Nuclei of cells in the lamina propria, cells which are below and surround the epithelial crypts, largely show hematoxylin blue- ...
Kober L, Zehe C, Bode J (April 2013). "Optimized signal peptides for the development of high expressing CHO cell lines". ... "Unconventional mechanisms of protein transport to the cell surface of eukaryotic cells". Annual Review of Cell and ... I. Detection in the microsomal membrane of a receptor for the signal recognition particle". The Journal of Cell Biology. 95 (2 ... Signal peptides function to prompt a cell to translocate the protein, usually to the cellular membrane. In prokaryotes, signal ...
The DNA was damaged in CHO cells as well as in mice. In mice (given 20 mg nivalenol /kg bw orally or 3.7 mg /kg bw ip) the DNA ... CHO) cells by slightly increased frequencies of chromosomal aberrations and sister chromatid exchange. ... NF-κB is a transcription factor that can be found in almost all human cells, and regulates the expression of its target genes ... Again, this effect is reduced by NF-κB inhibition which shows, that nivalenol and NF-κB interact to influence the cell. It was ...
The clastogen-suppressing effects of Tochu tea in CHO cells and mice". Mutation Research. 388 (1): 7-20. doi:10.1016/s1383-5718 ... inhibit AP-1 transactivation and cell transformation in the mouse epidermal JB6 cell line". Cancer Research. 61 (15): 5749-56. ...
Kober L, Zehe C, Bode J (April 2013). "Optimized signal peptides for the development of high expressing CHO cell lines". ... this gives cells the flexibility to adapt to a variable environment, external signals, damage to the cell, and other stimuli. ... Cell. 172 (4): 650-665. doi:10.1016/j.cell.2018.01.029. PMID 29425488. Grossman SR, Engreitz J, Ray JP, Nguyen TH, Hacohen N, ... Cell. 171 (7): 1573-1588.e28. doi:10.1016/j.cell.2017.11.008. PMC 5785279. PMID 29224777. Lambert SA, Jolma A, Campitelli LF, ...
Three primary expression systems have been established: EBNA-1-engineered CHO cell line CHO EBNA LT cell line, which is carried ... Recently, however, attempts to engineer CHO cells for transient protein production have garnered recognition. CHO cells were ... Codamo J, Munro TP, Hughes BS, Song M, Gray PP (June 2011). "Enhanced CHO cell-based transient gene expression with the epi-CHO ... Traditionally, Chinese hamster ovary (CHO) cells are associated with the establishment of stable cell lines for biologics. ...
Barron N, Kumar N, Sanchez N, Doolan P, Clarke C, Meleady P, O'Sullivan F, Clynes M (January 2011). "Engineering CHO cell ... "Alterations of microRNAs and their targets are associated with acquired resistance of MCF-7 breast cancer cells to cisplatin". ... a methylation-sensitive microRNA is involved in cell proliferation and invasion in human colorectal cancer". Carcinogenesis. 32 ... cellular motility and oxidative phosphorylation in neural precursors derived from human umbilical cord mesenchymal stem cells ...
Skalski M, Coppolino MG (Oct 2005). "SNARE-mediated trafficking of alpha5beta1 integrin is required for spreading in CHO cells ... Bves demonstrates dynamic localization, dependent upon cell-cell junction formation. Prior to cell-cell contact, Bves is ... Disruption of Bves results in decreased cell speed and increased cell roundness, which are cell processes modulated by the Rho ... Grossly, cell motility and cell adhesion are impaired. Only recently have the molecular mechanisms underlying the function of ...
"Antagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells". Mol. Pharmacol. 35 (4): 469-76. ...
"Antagonism of the five cloned human muscarinic cholinergic receptors expressed in CHO-K1 cells by antidepressants and ... "Antagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells". Mol. Pharmacol. 35 (4): 469-76. ... Acetylcholine (often abbreviated ACh) is a neurotransmitter whose receptors are proteins found in synapses and other cell ... "Decreased binding affinity of olanzapine and clozapine for human muscarinic receptors in intact clonal cells in physiological ...
Barron N, Kumar N, Sanchez N, Doolan P, Clarke C, Meleady P, O'Sullivan F, Clynes M (January 2011). "Engineering CHO cell ... "Differences in osteoblast miRNA induced by cell binding domain of collagen and silicate-based synthetic bone". Journal of ... "miR-489 is a tumour-suppressive miRNA target PTPN11 in hypopharyngeal squamous cell carcinoma (HSCC)". British Journal of ... "Functional profiling reveals critical role for miRNA in differentiation of human mesenchymal stem cells". PLOS ONE. 4 (5): ...
2015-03-04). "Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells". mAbs. 7 (2): 403-12. ... Therefore, up to 50% of F2A-linked proteins can remain in the cell as a fusion protein, which might cause some unpredictable ... 2011-04-29). Thiel V (ed.). "High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, ... April 2019). "Fusion of Reprogramming Factors Alters the Trajectory of Somatic Lineage Conversion". Cell Reports. 27 (1): 30-39 ...
This was followed by production of clinical grade alpha-glucosidase in Chinese hamster ovary (CHO) cells and in the milk of ... Genzyme also had its own product (Myozyme) grown in CHO cells under development. In November 2001, Genzyme chief executive ... muscle cells) or in white blood cells. The choice of sample depends on the facilities available at the diagnostic laboratory.[ ... Administration of the enzyme lead to its uptake by the liver and not the muscle cells where it is needed. In the early 1990s ...
September 2001). "Metastin suppresses the motility and growth of CHO cells transfected with its receptor". Biochemical and ... "Transcriptional expression of genes involved in cell invasion and migration by normal and tumoral trophoblast cells". The ... April 2014). "Glucagon regulates hepatic kisspeptin to impair insulin secretion". Cell Metabolism. 19 (4): 667-681. doi:10.1016 ... Journal of Cell Science. 117 (Pt 8): 1319-1328. doi:10.1242/jcs.00971. PMID 15020672. "KiSS1-Derived Peptide Receptors". IUPHAR ...
Kim YS, Jung JA, Kim HJ, Ahn YH, Yoo JS, Oh S, Cho C, Yoo HS, Ko JH (2011). "Galectin-3 binding protein promotes cell motility ... PTPkappa mediates homophilic cell-cell aggregation via its extracellular domain. PTPkappa only mediates binding between cells ... The reduction of PTPkappa expression in CHO cells with PTPkappa siRNA increased EGFR phosphorylation. Therefore, the hypothesis ... In these cells, adhesion to matrigel, transwell migration, and cell growth were all increased following the reduction of PTPRK ...
2004). "Mass spectrometry analysis of recombinant human ZP3 expressed in glycosylation-deficient CHO cells". Biochemistry. 43 ( ... Cell. 143 (3): 404-15. doi:10.1016/j.cell.2010.09.041. PMID 20970175. S2CID 18583237. PDB: 3NK3​ PDB: 3NK4​ Jovine L, Qi H, ... Cell. 143 (3): 404-15. doi:10.1016/j.cell.2010.09.041. PMID 20970175. S2CID 18583237. PDB: 3NK3, 3NK4​ Bansal P, Chakrabarti K ... Cell. Biol. 22 (9): 3111-20. doi:10.1128/MCB.22.9.3111-3120.2002. PMC 133755. PMID 11940668. ZP3+protein,+human at the U.S. ...