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*  Droplet-based microfluidics
Droplet-based microfluidic systems can be coupled to separation methods for specific tasks. Common separation techniques ... One of the key advantages of droplet-based microfluidics is the ability to use droplets as incubators for single cells. Devices ... ╬╝CGE devices are also practical for separation purposes because they use online separation, characterization, encapsulation, ... For example, 2D separation (two-dimensional chromatography) is possible with these devices (i.e. HPLC x LC, LC x LC, and HPLC x ...
*  Polygalacturonase
An additional complication is the difference in PG enzymatic activity between fruit and leaf cell-separation zones. In peach, ... Phytopathogenic fungi expose plant cell walls to cell wall degrading enzymes (CWDEs) like PGs. In response, most plants have ... Pectin is one of the three polysaccharides present in the plant cell wall, and it plays a role in maintaining the barrier ... Specifically, pectin in the middle lamella holds neighboring cells together. Fruit ripening The first GM food available in ...
*  Immunomagnetic separation
... (IMS) is a method that deals with the isolation of cells, proteins, and nucleic acids within a cell ... Immunomagnetic separation (IMS) is a laboratory tool that can efficiently isolate cells out of body fluid or cultured cells. It ... A mixture of cell population will be put into a magnetic field where cells then are attached to super paramagnetic beads, ... between magnetized beads coated materials are cleavable DNA linker cell separation from the beads when the culturing of cells ...
*  Dynabeads
Cell separation: fundamentals, analytical and preparative methods. Springer. ISBN 978-3-540-75262-2. Bead separation Ebook. ... Cell-types often of interest to purify may be specific leukocytes, such as CD4+ T cells, stem cells, or circulating tumor cells ... "The History of Dynal and Biomagnetic Separation". Invitrogen. Archived from the original on May 27, 2010. "Cell Isolation & ... Immunomagnetic cell sorting--pushing the limits. Thiel A, Scheffold A, Radbruch A. Immunotechnology. 1998 Oct;4(2):89-96. ...
*  Ficoll
This separation allows easy harvest of PBMCs. Note that some red blood cell trapping (presence of erythrocytes & granulocytes) ... to prevent clotting and is used as a mononuclear cell separator. At one time, fairly early in the research for diabetes ... a layer of mono-nuclear cells called buffy coat (PBMC/MNC), Ficoll-Paque, and erythrocytes & granulocytes which should be ...
*  Hydrodynamic trapping
This can lead to deformability-selective cell separation. This technique is especially useful to identify cancerous cells, ... Several approaches were applied in microfluidics facilitating single cell trapping for single cell analysis, which can be ... This way, cells which may be affected due to the presence of an external field/force are preserved. Apart from the simplicity ... The way target cells are separated in a solution depends on several types of effects. The first is inertial effects. The ...
*  Counterflow centrifugation elutriation
Since cell size is correlated with cell cycle stages this method also allows the separation of cells at different stages of the ... lyses of cell membrane) effect on the cells, so the cell will remain unchanged before and after the separation. Because of this ... so the speed of separation is fast. In summary: Minimum effect on the cells High recovery viability Separated cells can be used ... During the separation, the cell only needs to be suspended in a buffer solution and enter a centrifuge, the whole processes ...
*  Cell sorting
SEP system provides a column-free cell separation technique in which a tube of labeled cells is placed inside a magnetic field ... Three major types of cell sorting are fluorescent activated cell sorting, magnetic cell selection and single cell sorting. ... "Cell Separation Terminology, Uses, Methods and Technologies". Akadeum Life Sciences. Retrieved 2017-09-11. - Magnetic Bead Cell ... Cell sorting is a method used to separate cells isolated from an organisms tissues according to their type. Cells are mostly ...
*  Microfluidic whole genome haplotyping
Other automated techniques for metaphase cell separation would improve throughput. In addition, this method is only applicable ... to cells in metaphase, which inherently limits the technique to cell types and tissues that undergo mitosis. Single cell ... A single metaphase cell is isolated from solution. The chromosomes are then released from the nucleus, and the cytoplasm is ... Finally, since this entire process is based on amplification from a single cell, the accuracy of any genetic analysis is ...
*  Comparison of mobile phone standards
GSM uses TDMA and FDMA for user and cell separation. UMTS, IS-95 and CDMA-2000 use CDMA. WiMAX and LTE use OFDM. Time-division ... In effect, CDMA cell coverage decreases as the number of active users increases. This is called cell breathing. Less signal ... This is used in GSM to separate cells, which then use TDMA to separate users within the cell. Code-division multiple access ( ... After this if the noise still rises (more people join the party/cell) the listener cannot make out what the talker is talking ...
*  Circulating tumor cell
"Computational Analysis of Microfluidic Immunomagnetic Rare Cell Separation from a Particulate Blood Flow". Analytical Chemistry ... To obtain vital cells and to reduce stress of those cells, blood cells are prepared by only one centrifugation step and ... This method involves technology to separate nucleated cells from red blood cells, which lack a nucleus. All nucleated cells, ... Only vital, propidium excluding EpCAM positive cells are counted as potential tumor cells. Only living cells can grow into ...
*  Carbohydrate-binding module
Kuranda, M. J.; Robbins, P. W. (1991). "Chitinase is required for cell separation during growth of Saccharomyces cerevisiae". ... CBMs are the most common non-catalytic modules associated with enzymes active in plant cell-wall hydrolysis. Many putative CBMs ... In anaerobic bacteria that degrade plant cell walls, exemplified by Clostridium thermocellum, the dockerin domains of the ... Carbohydrate-binding module family 32 (CBM32) binds to diverse substrates, ranging from plant cell wall polysaccharides to ...
*  Cedarlane Laboratories
Cedarlane sells density-gradient cell separation media under the Lympholyte trade name. Cedarlane offers cell line platforms ... Cedarlane's manufactured products include monoclonal antibodies, polyclonal antibodies, cell separation media, complement for ...
*  Yeast
Yeong FM (2005). "Severing all ties between mother and daughter: cell separation in budding yeast". Molecular Microbiology. 55 ... Here, a small bud (also known as a bleb), or daughter cell, is formed on the parent cell. The nucleus of the parent cell splits ... The bud continues to grow until it separates from the parent cell, forming a new cell. The daughter cell produced during the ... The dying yeast cells are then heated to complete their breakdown, after which the husks (yeast with thick cell walls that ...
*  InGeneron
... develops, manufactures, licenses and sells regenerative cell separation and diagnostics tools. InGeneron's ... tissue prior to stem cell extraction and uses proprietary agitation to assist in the separation of the regenerative cells. This ... In 2015, the German-based stem cell banking company eticur) was acquired with the idea of supporting eticur) with scientific ... This system relies on a proprietary enzyme blend and centrifuge technology to isolate and prepare regenerative cells with high ...
*  Cell fractionation
Cell disruption Media for cell separation by density: Percoll Ficoll Alberts, B; Johnson, A. "Fractionation of Cells". ... It is the formation of homogenous mass of cells (cell homogenate or cell suspension). It involves grinding of cells in a ... Cell fractionation is the process used to separate cellular components while preserving individual functions of each component ... This step may not be necessary depending on the source of the cells. Animal tissue however is likely to yield connective tissue ...
*  Extensin
In: Cell Separation in Plants, edited by D.J. Osborne, et al, pp. 101-113. Springer-Verlag, Berlin. Schnabelrauch,L.S., ... Extensins are thought to act as self-assembling amphiphiles essential for cell-wall assembly and growth by cell extension and ... Lamport, D.T.A. (1965) Advances in Botanical Research 2:151-218 The protein component of primary cell walls Lamport,D.T.A.; ... Lamport,D.T.A. (1963) Oxygen fixation into hydroxyproline of plant cell wall protein. J.Biol.Chem., 238, 1438-1440. Lamport,D.T ...
*  Cell synchronization
Physical fractionation or cell separation techniques, based on the following characteristics are in use. Cell density Cell size ... The cell separation is carried out in a specially designed centrifuge and rotor. Fluorescence-activated cell sorting (FACS) is ... Cell Synchronization is a process by which cells at different stages of the cell cycle in a culture are brought to the same ... "Cell synchrony" is required to study the progression of cells through the cell cycle. The types of synchronizations are broadly ...
*  Erythrocytapheresis
This blood separation is also used in patients with severe malaria. After removal of the affected cells, the patient can then ... The centrifugal force will separate the red blood cells from other cells due to their high specific weight. These cells can ... For the separation of erythrocytes, whole blood is passed through an apparatus that isolates the red blood cells from the ... In addition, blood separation on microdevices containing capillary channels has been identified as a potential blood cell ...
*  Cells4Life
For volume-reduced service, samples are additionally processed in a closed cell separation system designed to remove the plasma ... Since stem cells can repair or replace almost any cell in the body, their storage may eliminate the need to search for a ... It is also the second private firm behind Smart Cells in the UK to provide umbilical stem cell storage and collection services ... Stem cells could be used to treat over 80 illnesses and are currently being used on clinical trials for cerebral palsy, hearing ...
*  Saccharomyces cerevisiae
Both mother and daughter cells can initiate bud formation before cell separation has occurred. In yeast cultures growing more ... The second reason is that haploid cells of one mating type, upon cell division, often produce cells of the opposite mating type ... Two haploid yeast cells of opposite mating type can mate to form diploid cells that can either sporulate to form another ... Similarly, stem cells use asymmetric division for self-renewal and differentiation. For many cells, M phase does not happen ...
*  Septum (cell biology)
... and the primary septum degraded during cell separation. "Cell Division: Stages of Mitosis , Learn Science at Scitable". www. ... A septum in cell biology is the new cell wall that forms between two daughter cells during the telophase of cell division. In ... Cabib E; Roh DH; Schmidt M; Crotti LB; Varma A (2001). "The yeast cell wall and septum as paradigms of cell growth and ... Lesage G; Bussey H (2006). "Cell wall assembly in Saccharomyces cerevisiae". Microbiol. Mol. Biol. Rev. 70 (2): 317-43. doi: ...
*  Magnetic nanoparticles
This technology is potentially relevant to cellular labelling/cell separation, detoxification of biological fluids, tissue ... Magnetic nanoparticles target human cancer cells Magnetic Nanoparticles Remove Ovarian Cancer Cells from the Abdominal Cavity ... Thanks to the easy separation by applying a magnetic field and the very large surface to volume ratio, magnetic nanoparticles ... In the case of magnetic nanoparticles it adds the property of facile a separation. An early example involved a rhodium ...
*  Miltenyi Biotec
The company offers solutions covering techniques of sample preparation, cell separation, cell sorting, flow cytometry, cell ... NK cells, dendritic cells, stem cells, tumor cells and neural cells. Isolated, adult progenitor / stem cell populations that ... The selected stem cells, monocytes, DCs, NK cells, B cells and T cell subsets allow research in the fields of regenerative ... cell separation, cell sorting, flow cytometry, molecular applications, cell culture up to GMP grade, preclinical imaging, ...
*  Lab-on-a-chip
Manufacturing and wetting low-cost microfluidic cell separation devices, Biomicrofluidics 7, 056501 (2013); doi:10.1063/ ... Recently such a cytometer was developed for just $5. Another active area of LOC research is for controlled separation and ... Sub-micrometre and nano-sized channels, DNA labyrinths, single cell detection and analysis, and nano-sensors, might become ... Biotechnology portal Molecular and cellular biology portal Biochemical assays Dielectrophoresis: detection of cancer cells and ...
*  STS-47
Life sciences included experiments on human health, cell separation and biology, developmental biology, animal and human ... Test subjects included the crew, Japanese koi fish (carp), cultured animal and plant cells, chicken embryos, fruit flies, fungi ...
*  Magnetic-activated cell sorting
... (MACS) is a method for separation of various cell populations depending on their surface ... S. Miltenyi, W. Muller, W. Weichel, and A. Radbruch, "High Gradient Magnetic Cell Separation With MACS," Cytometry, vol. 11, no ... Afterwards the cell solution is transferred on a column placed in a strong magnetic field. In this step, the cells attached to ... After administration of the cells/magnetic nanoparticles solution onto the column the cells expressing these antigens bind to ...