A cell-free system is an in vitro tool widely used to study biological reactions that happen within cells apart from a full ... where a cell-free translation system based on Escherichia coli (E. coli), of the cell extract-based type, had the mRNA template ... Cell-free systems may be divided into two primary classifications: cell extract-based, which remove components from within a ... Notably, in work leading to a Nobel prize the Nirenberg and Matthaei experiment used a cell-free system, of the cell extract- ...
... is the production of protein using biological machinery in a cell-free system, that is, without the use of living cells. The in ... "High-Level Cell-Free Production of Membrane Proteins with Nanodiscs". Cell-Free Protein Synthesis. Methods in Molecular Biology ... They used a cell-free system to translate a poly-uracil RNA sequence (or UUUUU... in biochemical terms) and discovered that the ... Spirin, A.; Baranov, V.; Ryabova, L.; Ovodov, S.; Alakhov, Y. (1988). "A continuous cell-free translation system capable of ...
"Establishing a Cell-free Vibrio natriegens Expression System". ACS Synthetic Biology. 7 (10): 2475-2479. doi:10.1021/acssynbio. ... Recently, V. natriegens crude extract has been shown by multiple research groups to be a promising platform for cell-free ... Des Doye, BJ; Davidson, SR; Weinstock, MT; Gibson, DG; Jewett, MC (2018-09-06). "Establishing a High-Yielding Cell-Free Protein ... Wiegand, Daniel J.; Lee, Henry H.; Ostrov, Nili; Church, George M. (2019-03-15). "Cell-free Protein Expression Using the ...
Lamfrom, Hildegard (June 1961). "Factors determining the specificity of hemoglobin synthesized in a cell-free system". Journal ... "The Synthesis of Hemoglobin in a Cell-Free System". Proc. Natl. Acad. Sci. U.S.A. 44 (10): 1029-1035. Bibcode:1958PNAS... ... An experimental system was developed in which an intron-containing rRNA precursor from the nucleus of the ciliated protozoan ... doi:10.1016/j.cell.2009.01.035. PMC 2675692. PMID 19239886. Bonasio, R.; Tu, S.; Reinberg, D. (2010). "Molecular Signals of ...
"A cell-free protein synthesis system for high-throughput proteomics". Proceedings of the National Academy of Sciences. 99 (23 ... In molecular biology, wheat germ extract is used to carry out cell-free in vitro translation experiments since the plant embryo ...
Establishment of cell-free protein synthesis system for practical usage. 2004 - Atushi Miyawaki. The multidisciplinary ... Creation of cell sheet engineering based on intelligent surfaces. 2010 - Teruhiko Wakayama [ja]. Development of novel animal ... The research and development of Dy free Nd-Fe-B anisotropic bonded magnet and its applications to motors. 2013 - Hiroshi Harada ... High-level synthesis and verification on system LSI. 2005 - Mitsuo Usami, Ryo Imura. Development of ultra-small chip for IC ...
... is a Japanese biochemist and professor well known for developing the cell-free protein expression system based on ... Endo, Yaeta (11 May 2021). "Development of a cell-free protein synthesis system for practical use". Proceedings of the Japan ... Sawasaki, Tatsuya; Ogasawara, Tomio; Morishita, Ryo; Endo, Yaeta (12 November 2002). "A cell-free protein synthesis system for ... Madono, Masaki; Sawasaki, Tatsuya; Morishita, Ryo; Endo, Yaeta (April 2011). "Wheat germ cell-free protein production system ...
"Development of Solid-State Storage for Cell-Free Expression Systems". ACS Synthetic Biology. doi:10.1021/acssynbio.3c00111. ... These are a vacuum chamber, shelves, process condenser, shelf-fluid system, refrigeration system, vacuum system, and control ... Cell extracts that support cell-free biotechnology applications such as point-of-care diagnostics and biomanufacturing are also ... "Thermostable lyoprotectant-enhanced cell-free protein synthesis for on-demand endotoxin-free therapeutic production". New ...
Boman HG, Faye I, Gudmundsson GH, Lee JY, Lidholm DA (October 1991). "Cell-free immunity in Cecropia. A model system for ... Boman HG, Hultmark D (1987). "Cell-free immunity in insects". Annual Review of Microbiology. 41: 103-26. doi:10.1146/annurev.mi ... A study in which cecropin genes were expressed in a human bladder carcinoma cell line showed that tumor cells bearing cecropin ... leading to cancer cell death. These same genes did not show significant expression changes in healthy cells upon treatment with ...
EFFECT OF TOXIN ON AMINO ACID INCORPORATION IN CELL-FREE SYSTEM". Journal of Experimental Medicine. 120 (6): 1019-1039. doi: ... doi:10.1016/j.cell.2013.03.040. ISSN 0092-8674. PMC 3676968. PMID 23622245. Pope, M. R.; Saari, L. L.; Ludden, P. W. (August 5 ... For the next 15 years, it was thought to be the only enzyme capable of adding a chain of ADP-ribose in mammalian cells. During ... If a PARP becomes overactive the cell will have decreased levels of NAD+ cofactor as well as decreased levels of ATP and thus ...
"Spatial organization of cytokinesis signaling reconstituted in a cell-free system". Science. 346 (6206): 244-247. Bibcode: ... Timothy John Mitchison FRS is a cell biologist and systems biologist and Hasib Sabbagh Professor of Systems Biology at Harvard ... Cell. 166 (3): 637-650. doi:10.1016/j.cell.2016.06.051. ISSN 1097-4172. PMC 5082712. PMID 27471966. Eggert, Ulrike S.; ... "Cell Biology Tree - Timothy J. Mitchison". academictree.org. Mitchison, T. J. (2013). "A question of taste". Molecular Biology ...
1979-1980: The development of cell-free systems leads to the identification of complex sets of proteins called accessory ... 1977-1979: Roeder develops cell-free systems to better study transcription. Composed of the purified RNA polymerases and ... the first cell-specific coactivator, discovered by Roeder in 1992, is unique to immune system B cells. 1996: Roeder's ... or specific to one particular cell type. Roeder and colleagues introduce the concept of cell specificity after they demonstrate ...
"Four-base codon-mediated saturation mutagenesis in a cell-free translation system". Journal of Bioscience and Bioengineering. ... Hong SH, Kwon YC, Jewett MC (2014). "Non-standard amino acid incorporation into proteins using Escherichia coli cell-free ... September 2015). "Robust production of recombinant phosphoproteins using cell-free protein synthesis". Nature Communications. 6 ... Thus far, this system has only been shown to work in an in-vitro translation setting where the aminoacylation of the orthogonal ...
"Replication independent formation of extrachromosomal circular DNA in mammalian cell-free system". PLOS ONE. 4 (7): e6126. ... For example, muscle and liver cells contain more copies of mtDNA per mitochondrion than blood and skin cells do. Due to the ... Chloroplasts contain multiple copies of cpDNA and the number can vary not only from species to species or cell type to cell ... This type of ecDNA is commonly seen in cancer cells of various histologies, but virtually never in normal cells. ecDNA are ...
It can be expressed efficiently by a cell-free protein expression system. This enzyme belongs to the family of transferases, ... "Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes". The Journal ... Higashi Y, Strominger JL, Sweeley CC (June 1967). "Structure of a lipid intermediate in cell wall peptidoglycan synthesis: a ...
2006). "An efficient mammalian cell-free translation system supplemented with translation factors". Protein Expr. Purif. 46 (2 ... Cell. Biol. 24 (8): 3295-306. doi:10.1128/MCB.24.8.3295-3306.2004. PMC 381664. PMID 15060152. Van Haren K, van der Voorn JP, ... Cell. Biol. 20 (11): 3965-76. doi:10.1128/MCB.20.11.3965-3976.2000. PMC 85753. PMID 10805739. Anthony TG, Fabian JR, Kimball SR ... 1996). "T-cell activation leads to rapid stimulation of translation initiation factor eIF2B and inactivation of glycogen ...
Appanna DL, Grundy BJ, Szczepan EW, Viswanatha T (1984). "Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62 ...
2006). "An efficient mammalian cell-free translation system supplemented with translation factors". Protein Expr. Purif. 46 (2 ... Cell. Proteomics. 6 (4): 575-88. doi:10.1074/mcp.M600249-MCP200. PMID 17220478. Ewing RM, Chu P, Elisma F, et al. (2007). " ... Cell. 8 (1): 31-42. doi:10.1016/j.devcel.2004.10.018. PMID 15669143. Marques AC, Dupanloup I, Vinckenbosch N, et al. (2006). " ... of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells". Mol. ...
Appanna DL, Grundy BJ, Szczepan EW, Viswanatha T (1984). "Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62 ...
LAMFROM H. Factors determining the specificity of hemoglobin synthesized in a cell-free system. J Mol Biol. 1961 Jun;3:241-52. ... Lamfrom, Hildegard (1961-06-01). "Factors determining the specificity of hemoglobin synthesized in a cell-free system". Journal ... "Factors determining the specificity of hemoglobin synthesized in a cell-free system". Journal of Molecular Biology. 3 (3): 241- ... in a cell-free context. This allowed her to make a number of contributions to the field including providing some of the first ...
Gruenberg, J.; Howell, K. E. (1988). "Fusion in the endocytic pathway reconstituted in a cell-free system using immuno-isolated ... Using innovative approaches such as phospholipid-specific antibodies and reconstituted cell-free systems, Jean Gruenberg and ... "Reconstitution of vesicle fusions occurring in endocytosis with a cell-free system". The EMBO Journal. 5 (12): 3091-3101. doi: ... and motor-dependent fusion in vitro between apical and basolateral endocytic vesicles from MDCK cells". Cell. 62 (4): 719-731. ...
"Functional protein expression from a DNA based wheat germ cell-free system". J. Struct. Funct. Genomics. 8 (4): 199-208. doi: ... Promega was an early supplier in the cell-free protein synthesis field and is continuing to develop its portfolio in this area ... The company also sells their own Maxwell RSC and Maxwell RSC 48 Systems, bench-top automated purification systems for low and ... The Promega PowerPlex STR systems were the first commercially available systems for STR analysis that contained all of the ...
Chen CM, Melitz DK (1979). "Cytokinin biosynthesis in a cell-free system from cytokinin-autotrophic tobacco tissue cultures". ...
Jinnai H, Nakamura S (2000). "Characterization of phospholipase D activation by GM2 activator in a cell-free system". Kobe ... 1]. In melanocytic cells GM2A gene expression may be regulated by MITF. Mutations in this gene, inherited in an autosomal ... GM2A is a lipid transfer protein that stimulates the enzymatic processing of gangliosides, and also T-cell activation through ... 2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res. 21 (6): 665-76. doi ...
Reduction of aromatic acids to aldehydes and alcohols in the cell-free system. 2. Purification and properties of aryl-alcohol: ...
Jinnai H, Nakamura S (August 1999). "Characterization of phospholipase D activation by GM2 activator in a cell-free system". ... The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by the GPLD1 gene is a GPI degrading enzyme that ... Xiaotong H, Hannocks MJ, Hampson I, Brunner G (2002). "GPI-specific phospholipase D mRNA expression in tumor cells of different ... April 1994). "Release of GPI-anchored membrane proteins by a cell-associated GPI-specific phospholipase D". The EMBO Journal. ...
Gross GG, Zenk MH (1969). "[Reduction of aromatic acids to aldehydes and alcohols in the cell-free system. 1. Purification and ...
Clarke CA, Clarke PR (2005). "DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system". Biochem. J. 388 ( ... Cell. 23 (3): 319-29. doi:10.1016/j.molcel.2006.06.013. PMID 16885022. Mamely I, van Vugt MA, Smits VA, et al. (2006). "Polo- ... Cell. Biol. 26 (16): 6056-64. doi:10.1128/MCB.00492-06. PMC 1592810. PMID 16880517. Mailand N, Bekker-Jensen S, Bartek J, Lukas ... Cell. 23 (3): 307-18. doi:10.1016/j.molcel.2006.06.016. PMID 16885021. Peschiaroli A, Dorrello NV, Guardavaccaro D, et al. ( ...
Eukaryotic cell extracts may also be used in other cell-free systems, for example, the wheat germ cell-free expression systems ... Brödel AK, Wüstenhagen DA, Kubick S (2015). "Cell-Free Protein Synthesis Systems Derived from Cultured Mammalian Cells". ... Mammalian cell-free systems have also been produced. Expression vector in an expression host is now the usual method used in ... Wheat Germ Cell-Free Expression System for Protein Production". Current Protocols in Protein Science. Vol. Chapter 5. pp. 5.18. ...
Rohmer M, Anding C, Ourisson G (December 1980). "Non-specific biosynthesis of hopane triterpenes by a cell-free system from ... These cold- and desiccation-resistant cell structures are dormant and therefore not photosynthetically active, further ... Welander PV (August 2019). "Deciphering the evolutionary history of microbial cyclic triterpenoids". Free Radical Biology & ... methods and systems", issued 2016-10-19, assigned to California Institute of Technology (Articles with short description, Short ...
"A Ubiquitous Amino Acid Source for Prokaryotic and Eukaryotic Cell-Free Transcription-Translation Systems". Frontiers in ... leaving many smaller peptide chains alongside the free amino acids. Casamino acid is used as a component of microbiological ...