Loading...
*  Far-western blotting
... blotting' step. In a western blot, specific proteins are then identified using an antibody probe. Far-western blotting employs ... Far-western blotting is a molecular biological method which is based on the technique of western blotting to detect protein- ... Whereas western blotting uses an antibody to detect a protein of interest, far-western blotting uses a non-antibody protein, ... Thus, whereas western blotting is used for the detection of certain proteins, far-western blotting is rather employed to detect ...
*  Western blot
Far-eastern blotting Far-western blotting Eastern blot Northwestern blot SDS-PAGE Fast parallel proteolysis (FASTpp) ... see western blot normalization) The colorimetric detection method depends on incubation of the western blot with a substrate ... a western blot is sometimes used to confirm FIV+ status in cats.[citation needed] Further applications of the western blot ... Some forms of Lyme disease testing employ western blotting.[citation needed] A western blot can also be used as a confirmatory ...
*  Western blot normalization
"Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS)". PLoS ... Normalization of Western blot data is an analytical step that is performed to compare the relative abundance of a specific ... When using fluorescent antibodies to image proteins in western blots, normalization requires that the user define the upper and ... Taylor, SC; Berkelman, T; Yadav, G; Hammond, M (2016-08-23). "A defined methodology for reliable quantification of Western blot ...
*  Antibody
Kurien B, Scofield R (2006). "Western blotting". Methods. 38 (4): 283-293. doi:10.1016/j.ymeth.2005.11.007. PMID 16483794. ... in Western blot analyses to identify proteins separated by electrophoresis, and in immunohistochemistry or immunofluorescence ... Western blot, immunodiffusion, immunoelectrophoresis, and magnetic immunoassay. Antibodies raised against human chorionic ... Monoclonal antibody Neutralizing antibody Secondary antibodies Single-domain antibody Slope spectroscopy Western blot ...
*  Molecular biology
Using western blotting techniques allows not only detection but also quantitative analysis. Analogous methods to western ... In western blotting, proteins are first separated by size, in a thin gel sandwiched between two glass plates in a technique ... The terms northern, western and eastern blotting are derived from what initially was a molecular biology joke that played on ... Kurien, Biji T.; Scofield, R. Hal (1 April 2006). "Western blotting". Methods (San Diego, Calif.). 38 (4): 283-293. doi:10.1016 ...
*  Polyacrylamide gel electrophoresis
Western blotting is a process by which proteins separated in the acrylamide gel are electrophoretically transferred to a stable ... Western blot). After staining, different species biomolecules appear as distinct bands within the gel. It is common to run ... Agarose gel electrophoresis Capillary electrophoresis DNA electrophoresis Eastern blotting Electroblotting Fast parallel ... Isoelectric focusing Isotachophoresis Native gel electrophoresis Northern blotting Protein electrophoresis Southern blotting ...
*  Lysis buffer
... western blot). Most lysis buffers contain salts (e.g. Tris-HCl or EDTA) to regulate the acidity and osmolarity of the lysate. ... "Sample preparation for western blot , Abcam". www.abcam.com. Retrieved 2016-03-16. https://www.thermofisher.com/order/catalog/ ...
*  Image Studio Lite
"Western Blot Quantification with LI-COR Image Studio Lite". How to WESTERN-BLOT. Retrieved 7 March 2014. "Densitometry Tutorial ... "Quantification of Western Blots Using ImageJ". How to WESTERN-BLOT. Retrieved 7 March 2014. "Image Studio Lite Software". licor ... The primary function of Image Studio Lite is to quantitate relative abundance of proteins on a Western blot or DNA/RNA in an ... Image Studio™ Lite is free image processing software used for quantitation of Western blot images and images from related ...
*  Amido black 10B
Western blot normalization "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017. MSDS at Oxford University. ... such as the western blot. It is also used in criminal investigations to detect blood present with latent fingerprints. It ... 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, ...
*  MChip
"ELISA/Western blot tests for HIV". Medline Plus. Retrieved 2011-08-16. ...
*  Transcription factor
The sample is detected on a western blot. By using electrophoretic mobility shift assay (EMSA), the activation profile of ...
*  Equine protozoal myeloencephalitis
Diagnosis of equine protozoal myeloencephalitis: Western blot analysis. Proc Am Coll Vet Intern Med Forum 587-590, 1993. ... A review: Determining the sensitivity and specificity of western blot tests for diagnosis of Equine protozoal myeloencephalitis ...
*  Electroblotting
Alternatively the proteins may react with ligands like lectins giving rise to the term affinity blotting. Western blotting SDS- ... 141-. ISBN 978-81-7993-379-4. United State Patent 4840714 Protein (Western) Blotting - Introduction to Antibodies Membrane ... Following electrophoresis, a standard tank or semi-dry blotting transfer system is set up. A stack is put together in the ... Because the proteins may retain or regain part of their structure during blotting they may react with specific antibodies ...
*  Thermal shift assay
Just as for FastPP the Western blot could be replaced by staining for a polyhistidine-tag in the SDS-PAGE gel. Additionally, ... Optionally, for instance for proteins expressed at low levels, a Western blot is then run to determine at what temperature a ... The presence of the target protein in each soluble fraction is determined by Western blotting and used to construct a CESTA ... To reduce the workload, Western Blots could be replaced by SDS-PAGE gel polyhistidine-tag staining, provided that the protein ...
*  Rocker (laboratory)
Retrieved on 31 Jan 2012 Western Blot Protocol. University of Idaho, College of Agricultural and Life Sciences. Retrieved on 31 ... Rockers are commonly used for staining and de-staining gels after electrophoresis, hybridization, washing, blotting, Cell ...
*  Lyme disease
ELISA screening and Western blot confirming); or 3) single-tier IgG (old infection) Western blot. Previously, the CDC only ... Studies show the Western blot IgM has a specificity of 94-96% for people with clinical symptoms of early Lyme disease. The ... Western blot, ELISA, and PCR can be performed by either blood test via venipuncture or cerebrospinal fluid (CSF) via lumbar ... The case definition now includes the use of Western blot without prior ELISA screen. The number of reported cases of the ...
*  Coomassie Brilliant Blue
Coomassie Blue staining can also be used as a loading control staining method in western blot analysis. It is applied as an ... Welinder, Charlotte; Ekblad, Lars (2011-03-04). "Coomassie Staining as Loading Control in Western Blot Analysis". Journal of ...
*  Epicocconone
... making it a powerful loading control for Western blots. In addition to the natural variant from the fungus, there are several ... A powerful loading control for Western blots". Proteomics. 14 (2-3): 162-8. doi:10.1002/pmic.201300089. PMID 24339236. Peixoto ...
*  Ponceau S
... sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on Western blots for blot- ... Coomassie Brilliant Blue Western blot normalization "Ponceau S solution". Sigma-Aldrich. Retrieved 2016-11-24. Al-Amoudi, M. S ... Western blotting), as well as on cellulose acetate membranes. A Ponceau S stain is useful because it does not appear to have a ...
*  Meir Wilchek
Examples include western blot, ELISA, ELISPOT and pull-down assays. More recently, Wilchek participated in structural studies ...
*  Calcitriol receptor
Signal requirements and characterization by western blots and DNA sequencing". J. Biol. Chem. 266 (12): 7588-95. PMID 1850412. ...
*  Multiplex (assay)
Ambroz K., (2006). "Improving quantitation accuracy for western blots".Image Analysis 09/2006.[1] Laxman B, Morris DS, Yu J, et ... for specific activities Multiplex detection enables the simultaneous detection of two or more targets on a western blot. ...
*  Phycobilisome
... versus cyanine dye detection capabilities in Western blot application. Light Harvesting by Phycobilisomes Annual ... Western blotting and protein microarrays. Some phycobilisomes have an absorption and emission profile similar to Cy5, they can ...
*  SH2D3A
The protein may be useful in Western Blots and ELISAs. SH2D3A has been shown to interact with the epidermal growth factor ...
*  Chemical specificity
Another technique that relies on chemical specificity is Western blotting, which is utilized to detect a certain protein of ... "An overview of technical considerations for Western blotting applications to physiological research". Scandinavian Journal of ...