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*  Southern blot
Other blotting methods (i.e., western blot, northern blot, eastern blot, southwestern blot) that employ similar principles, but ... acids Restriction fragment Genetic fingerprint Northern blot Western blot Eastern blot Southwestern blot Northwestern blot ... A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern ... Southern blots performed with restriction enzyme-digested genomic DNA may be used to determine the number of sequences (e.g., ...
*  Molecular biology
Named after its inventor, biologist Edwin Southern, the Southern blot is a method for probing for the presence of a specific ... Southern blotting is less commonly used in laboratory science due to the capacity of other techniques, such as PCR, to detect ... Brown, T. (1 May 2001). "Southern blotting". Current Protocols in Immunology. Chapter 10: Unit 10.6A. doi:10.1002/0471142735. ... The eastern blotting technique is used to detect post-translational modification of proteins. Proteins blotted on to the PVDF ...
*  Biomedicine
"Southern blot". Schopf, J. William. Life's Origin. University of California Press. ISBN 978-0-520-23391-1. The Cancer Genome ... Macromolecule blotting is a process performed after gel electrophoresis. An alkaline solution is prepared in a container. A ... and macromolecule blotting to manipulate DNA. Polymerase chain reaction is done by placing a mixture of the desired DNA, DNA ...
*  Mega-telomere
These large regions were termed "ultra-long" telomeres in the literature when they were identified using southern blotting and ... In slot blot (or dot blot), total genomic DNA is attached to a membrane and labeled with a telomere-probe that produces a ... Southern blot). Specialized protocols have demonstrated the ability to isolate high molecular weight Class III telomeric DNA ... Slot blot, however, is conducted without DNA fragmentation or separation, rather whole genomic DNA is used to quantify the ...
*  Gel electrophoresis of nucleic acids
This process is termed Southern blotting. For fluorescent dyes, after electrophoresis the gel is illuminated with an ...
*  DNA profiling
... this procedure is called a Southern blot. The DNA fragments within the blot are permanently fixed to the filter, and the DNA ... The Southern blot technique is laborious, and requires large amounts of undegraded sample DNA. Also, Karl Brown's original ... The blot is then exposed to an X-ray film. Fragments of DNA that have bound to the probe molecules appear as fluoresent bands ... Early assays such as the HLA-DQ alpha reverse dot blot strips grew to be very popular due to their ease of use, and the speed ...
*  Bulked segregant analysis
Bulked DNA samples can be analysed using Southern blotting. Use of restriction enzymes or PCR amplification on the DNA is ...
*  Tiling array
Historically, the method uses Southern blotting to find digested fragments. Tiling arrays have allowed researchers to apply the ...
*  Pearson syndrome
Diagnosing Pearson Syndrome utilizes leukocyte DNA with the Southern Blot analysis. This type of mitochondrial DNA deletion are ...
*  Phosphorus-32
This technique is extensively used in Southern blot analysis of DNA samples. In this case a phosphorus-32-containing DNA probe ...
*  Merkel cell polyomavirus
Detection of viral DNA is performed by PCR or by Southern blot. Caution is needed in interpreting results from PCR since it is ...
*  Reverse northern blot
Gene expression Northern Blot Southern Blot Differential display DNA Microarray qPCR RNA Seq Primrose, Sandy B.; Twyman, ... Reverse Northern blot, much like the northern blot upon which it is based, is used to determine levels of gene expression in ... While northern blot or q-PCR are often used to confirm results, both techniques have drawbacks. Northern blot is limited by its ... In comparison to the Northern blot, the reverse northern blot is able to probe a large number of transcripts at once with less ...
*  Bacteriophage T12
An image of this southern blot can be seen in this article. Bacteriophages are very robust biological agents, are very hard to ... Biochemical tests such as southern blots can also be used to detect the speA that the phage produces from the speA gene. This ...
*  Medical genetics
Southern blotting is an early technique basic on detection of fragments of DNA separated by size through gel electrophoresis ... Southern blotting is still useful in the diagnosis of disorders caused by trinucleotide repeats. Short tandem repeats are ...
*  Mycobacterium ulcerans
Southern blot analysis to detect IS2404 and IS2606 shows inconclusive RFLP patterns between different strains. Due to the high ... limiting the value of the Southern blot method to type M. ulcerans isolates. Jackson et al. have used pTBN12, a well-defined ...
*  Zoo blot
A zoo blot or garden blot is a type of Southern blot that demonstrates the similarity between specific, usually protein-coding ... A zoo blot compares animal species while a garden blot compares plant species. The purpose of the zoo blot is to detect the ... Southern blot Fluorescent in situ hybridization Khan, Firdos Alam (20 September 2011). Biotechnology Fundamentals. CRC Press. p ... As a result, zoo blotting is used to detect similar or exact relationships between the DNA in question and other organisms. It ...
*  Bt brinjal
The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' ...
*  Archaeogenetics
conducted southern blot hybridization on Neanderthal aDNA (extracted from fossil remain W-NW and Krapina). The results showed ... Studying haplogroups has led some scientists to conclude that a southern migration into the Americas from one small population ... Genetic analysis has supported archaeological hypotheses of a large-scale migrations of Bantu speakers into Southern Africa ...
*  Breus' mole
Evidence from Southern blot test reveals that 85 percent of the clotted material is maternal blood. Breus mole is reported to ...
*  PstI
Its use is not limited to molecular cloning; it is also used in restriction site mapping, genotyping, southern blotting, ...
*  Polysomy
Mutations in chromosome 4 are able to be visualized when RFLP is used in conjunction with Southern blot analysis. Human ...
*  Restriction enzyme
In a similar manner, restriction enzymes are used to digest genomic DNA for gene analysis by Southern blot. This technique ...
*  Northern blot
Southern blot Western blot Northwestern blot Far-Eastern blotting Far-western blotting Eastern blot Differential display ... Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist ... Northern blots can be searched by a blot ID, paper reference, gene identifier, or by tissue. The results of a search provide ... The northern blot, or RNA blot, is a technique used in molecular biology research to study gene expression by detection of RNA ...
*  Chimeric RNA
Using Southern blotting and fluorescence in situ hybridization (FISH) on the genome, the researchers found no evidence of DNA ...
*  Transposon mutagenesis
Colonies that underwent random transposition events were identified by BamHI digestion and Southern blotting using an internal ...