Loading...
*  Mixed inhibition
... seen in cases where the inhibitor favours binding to the free enzyme. More closely mimics competitive binding. An increase in ... in which the inhibitor can only bind the enzyme if the substrate has already bound. If the ability of the inhibitor to bind the ... it is known as a non-competitive inhibitor. Non-competitive inhibition is sometimes thought of as a special case of mixed ... a site different from the active site where the substrate binds. However, not all inhibitors that bind at allosteric sites are ...
*  Cocaine intoxication
"Competitive binding between cocaine and lidocaine". Journal of the American College of Cardiology. 27 (2): 80. doi:10.1016/ ... Cocaine pharmacodynamics involve multiple complex mechanisms, although its half-life is short (~ 1 hour). This drug binds and ...
*  Chromium(III) picolinate
Quarles, C.; Marcus, R.; Brumaghim, Julia (2011). "Competitive binding of Fe3+, Cr3+, and Ni2+ to transferrin". Journal of ... The bicarbonate ligand is crucial in binding Cr3+ as when bicarbonate concentrations are very low, the binding affinity is also ... Low-molecular-weight chromium-binding substance (LMWCr; also known as chromodulin) is an oligopeptide that seems to bind ... to bind as well. The binding sites consist of a C-lobe and an N-lobe which are nearly identical in structure. Each lobe ...
*  ELISA
A third use of ELISA is through competitive binding. The steps for this ELISA are somewhat different from the first two ... The steps are: A surface is prepared to which a known quantity of capture antibody is bound. Any nonspecific binding sites on ... In between the washes, only the ligand and its specific binding counterparts remain specifically bound or "immunosorbed" by ... thus ELISA falls under the bigger category of ligand binding assays. The ligand-specific binding reagent is "immobilized", i.e ...
*  Theories of general anaesthetic action
Franks NP, Lieb WR (August 1984). "Do general anaesthetics act by competitive binding to specific receptors?". Nature. 310 (16 ... Thus the volume of the n-alkanol chain at the cutoff length provides an estimate of the binding site volume. This objection ... It was initially hypothesized that general anaesthetic binds to its target ion channel by a key-lock mechanism and changes its ... General anaesthetics could equally well be binding to hydrophobic target sites on proteins in the brain. The main reason that ...
*  GRIN2B
Wyszynski M, Lin J, Rao A, Nigh E, Beggs AH, Craig AM, Sheng M (January 1997). "Competitive binding of alpha-actinin and ... Irie M, Hata Y, Takeuchi M, Ichtchenko K, Toyoda A, Hirao K, Takai Y, Rosahl TW, Südhof TC (September 1997). "Binding of ... a novel postsynaptic protein that binds to the third PDZ domain of PSD-95/SAP90". Neuron. 20 (4): 693-707. doi:10.1016/s0896- ... The NR2 subunit acts as the agonist binding site for glutamate, one of the predominant excitatory neurotransmitter receptors in ...
*  Actinin alpha 2
Wyszynski M, Lin J, Rao A, Nigh E, Beggs AH, Craig AM, Sheng M (January 1997). "Competitive binding of alpha-actinin and ... Wyszynski M, Lin J, Rao A, Nigh E, Beggs AH, Craig AM, Sheng M (January 1997). "Competitive binding of alpha-actinin and ... Galliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E (May 2000). "Binding of ADAM12, a marker of skeletal muscle ... Galliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E (May 2000). "Binding of ADAM12, a marker of skeletal muscle ...
*  RecF pathway
Demonstration of competitive binding and the lack of a specific protein-protein interaction". Journal of Biological Chemistry. ... When present, SSBP binds to the 3' overhang remaining after RecJ finishes cutting back the 5' strand. By binding to the single- ... In the RecFOR pathway, the RecFR complex binds where the single-strand DNA of the 3' meets the double-strand DNA. RecO then ... The RecF pathway begins when RecJ, an exonuclease that cleaves single-stranded DNA in the 5 → 3′ direction, binds to the 5' end ...
*  EFR (EF-Tu receptor)
This has been proven analytically through competitive binding assays and SDS-PAGE analysis. When EFR binds to EF-Tu, the basal ... Instead of binding to EF-Tu, it binds to flagellin, another highly conserved structure present on many pathogens. It, like EF- ... This receptor is an important part of the plant immune system as it allows the plant cells to recognize and bind to EF-Tu, ... They are part of the innate immune system and bind to and prevent the proliferation of pathogens with the PAMPs that they can ...
*  K-server problem
... reducing the competitive ratio to k or providing an improved lower bound remains open as of 2014[update]. The most common ... a randomized algorithm with competitive bound Õ(log2k log3n) was found. To make the problem more concrete, imagine sending ... The competitive ratio of our algorithm on this input is 20,500/6000 or approximately 3.4, and by adjusting the parameters of ... 1990) first proved that there exists an algorithm with finite competitive ratio for any constant k and any metric space, and ...
*  Drug test
Urine drug testing is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine ... A drug, if present in the urine specimen below its cut-off concentration, will not saturate the binding sites of its specific ... specimen compete against their respective drug conjugate for binding sites on their specific antibody. During testing, a urine ...
*  Thymidylate synthase
FdUMP is able to inhibit TS by binding to the nucleotide-binding site of dUMP. This competitive binding inhibits the normal ... which acts as an antimetabolite that irreversibly inhibits TS by competitive binding. However, due to a low level of 5-FU found ... A cysteine residue is involved in the catalytic mechanism (it covalently binds the 5,6-dihydro-dUMP intermediate). The sequence ...
*  Optical mapping
Later technologies use DNA melting, DNA competitive binding or enzymatic labelling in order to create the optical mappings. The ... "Competitive binding-based optical DNA mapping for fast identification of bacteria - multi-ligand transfer matrix theory and ...
*  2-Methoxyestradiol
Thekkumkara T, Snyder R, Karamyan VT (2016). "Competitive Binding Assay for the G-Protein-Coupled Receptor 30 (GPR30) or G- ... It also acts as a vasodilator.[citation needed] 2-ME2 is derived from estradiol, although it binds poorly (2000-fold lower ...
*  2-Hydroxyestradiol
Thekkumkara, Thomas; Snyder, Russell; Karamyan, Vardan T. (2016). "Competitive Binding Assay for the G-Protein-Coupled Receptor ... Moreover, 2-hydroxyestradiol has been found to bind to the α1-adrenergic receptor with slightly more than half the affinity of ... It was noted that this could be due to 2-hydroxyestradiol binding to and antagonizing the D2 receptor. However, the researchers ... Kuiper GG, Carlsson B, Grandien K, Enmark E, Häggblad J, Nilsson S, Gustafsson JA (1997). "Comparison of the ligand binding ...
*  Butylparaben
... displayed the most competitive binding to rat estrogen receptors when tested along with methyl, ethyl, and ...
*  Nuclear receptor
However they block the effect of agonist through competitive binding to the same binding site in the nuclear receptor. These ... Type IV nuclear receptors bind either as monomers or dimers, but only a single DNA binding domain of the receptor binds to a ... C) DNA-binding domain (DBD): Highly conserved domain containing two zinc fingers that binds to specific sequences of DNA called ... Binding of antagonist ligands to nuclear receptors in contrast induces a conformation of the receptor that preferentially binds ...
*  Arachidonate 5-lipoxygenase
"Competitive binding assay of src homology domain 3 interactions between 5-lipoxygenase and growth factor receptor binding ... ALOX5 binds with the F actin-binding protein, coactin-like protein. Based on in vitro studies, this protein binding serves to ... may serve as a mobile lid over ALOX5's substrate-binding site An Adenosine triphosphate (ATP) binding site; ATP is crucial for ... The binding of ALOX5 to membranes as well as its interaction with FLAP likewise cause the enzyme to alter its relative levels ...
*  GRB2
"Competitive binding assay of src homology domain 3 interactions between 5-lipoxygenase and growth factor receptor binding ... The C-terminal SH3 domain binds to peptides conforming to a P-X-I/L/V/-D/N-R-X-X-K-P motif that allows it to specifically bind ... Zamora-Leon SP, Lee G, Davies P, Shafit-Zagardo B (Oct 2001). "Binding of Fyn to MAP-2c through an SH3 binding domain. ... The N-terminal SH3 domain binds to proline-rich peptides and can bind to the Ras-guanine exchange factor SOS. ...
*  Irazepine
It is a non-competitive benzodiazepine binding site antagonist. Irazepine and other alkylating benzodiazepine, kenazepine, bind ... to brain benzodiazepine receptors in a non-competitive (covalent) fashion in vitro, and may exert a long-lasting anticonvulsant ...
*  Adduct
... upon Small-Molecule Binding and the Competitive Binding of CO and Ethylene". Journal of the American Chemical Society. 128 (41 ...
*  Antibiotic use in livestock
MOS works as a competitive binding site, as bacteria bind to it rather than the intestine and are carried out. Bacteriophages ... In another study it was found that using probiotics, competitive exclusion, enzymes, immunomodulators and organic acids ...
*  Quorum sensing
... through its competitive binding to LsrR. Glyceraldehyde 3-phosphate has also been shown to inhibit the lsr operon through cAMP- ... When gram-positive bacteria detect high concentration of AIP in the environment, AIP bind to a receptor to activate kinase. The ... Another possible mechanism is that AIP is transported into the cytosol, and binds directly to a transcription factor to ... Usually AHLs do not need additional processing, and bind directly to transcription factors to regulate gene expression. Some ...
*  Dissociation constant
Acid Equilibrium constant Ki Database Competitive inhibition pH Scatchard plot Ligand binding Bisswanger, Hans (2008). Enzyme ... Then, the concentration of bound ligands [ L ] bound {\displaystyle {{\ce {[L]_{bound}}}}} becomes [ L ] bound = n [ M ] 0 [ L ... bound = [ LM ] + 2 [ L 2 M ] + 3 [ L 3 M ] + ⋅ ⋅ ⋅ + n [ L n M ] {\displaystyle {\ce {[L]_{bound}={[LM]}+{2[L_{2}M]}+{3[L_{3}M ... though the derivation can be extended to explicitly allow for and describe competitive binding.[citation needed] It is useful ...
*  WDFW Toxics in Biota fish monitoring
Quantification of vitellogenin is performed by measuring competitive binding to an enzyme in the presence of another ...