Viridans streptococci

Some taxonomists have lumped the S. sanguinis group in with the S. mitis group based on 16S rRNA gene sequence analysis, but S ... sequence homology in this gene. In light of the high degree of 16S rRNA gene sequence similarity, sequencing of alternative ... Other sequence-based identification systems have subsequently been introduced for VGS species level identification. In general ... Sequence-based identification. Historically, DNA-DNA hybridization studies have been used to confirm species level ...

NASBA (molecular biology)

Nucleic acid sequence based amplification (NASBA) is a method in molecular biology which is used to amplify RNA sequences. ... Mugasa, CM; Laurent, T; Schoone, GJ; Kager, PA; Lubega, GW; Schallig, HD (2009). "Nucleic acid sequence-based amplification ... Compton, J (1991). "Nucleic acid sequence-based amplification". Nature. 350 (6313): 91-2. doi:10.1038/350091a0. PMID 1706072. ... "Real-time nucleic acid sequence-based amplification is more convenient than real-time PCR for quantification of Plasmodium ...

Thomas J. Kelly (scientist)

Base sequence of the recognition site". Journal of Molecular Biology. 51 (2): 393-409. doi:10.1016/0022-2836(70)90150-6. PMID ... Kelly determined the DNA sequences recognized by type II restriction enzymes, which subsequently became major tools in ...

Glossary of biology

whole genome sequencing . wobble base pair . wood The inner layer of the stems of woody plants, composed of xylem. xanthophyll ... nucleotide bases are matched to synthesize the new partner strands. DNA sequencing . dynein A motor protein (also called ... Biomass can be used as a source of energy and it most often refers to plants or plant-based materials which are not used for ... nucleic acid . nucleic acid sequence . nucleobase . nucleoid . nucleolus . nucleotide . Contents: 0-9 A B C D E F G H I J K L M ...

List of RNA structure prediction software

Meyer F, Kurtz S, Backofen R, Will S, Beckstette M (2011). "Structator: fast index-based search for RNA sequence-structure ... Andrew Xiang Li; Jing Qin; Manja Marz; Christian M. Reidys (2011). "RNA-RNA interaction prediction based on multiple sequence ... Weinberg Z, Ruzzo WL (2006). "Sequence-based heuristics for faster annotation of non-coding RNA families". Bioinformatics. 22 ( ... R Kleinkauf; M Mann; R Backofen (2015). "antaRNA: ant colony-based RNA sequence design". Bioinformatics. 31 (19): 3114-3121. ...

Neighbor joining

Xavier Didelot (2010). "Sequence-Based Analysis of Bacterial Population Structures". In D. Ashley Robinson; Daniel Falush; ... Usually used for trees based on DNA or protein sequence data, the algorithm requires knowledge of the distance between each ... Based on a distance matrix relating the n {\displaystyle n} taxa, calculate Q {\displaystyle Q} as follows: where d ( i , j ... Based on the current distance matrix calculate the matrix Q {\displaystyle Q} (defined below). Find the pair of distinct taxa i ...

Mascot (software)

Based on the peptide sequence tag approach. MS/MS Ion Search Identify fragment ions from uninterpreted MS/MS data of one or ... Mascot generates a randomized sequence of the same length for every sequence in the target database. The decoy sequence is ... Perkins DN, Pappin DJ, Creasy DM, Cottrell JS (December 1999). "Probability-based protein identification by searching sequence ... Sequence query Combines peptide mass data with amino acid sequence and composition information usually obtained from MS/MS ...

Blackish deer mouse

2000). "Phylogeography of Peromyscus furvus (Rodentia; Muridae) based on cytochrome b sequence data". Molecular Ecology. 9 (12 ...

Leucanthemum

Compositae, Anthemideae) based on cpDNA sequence variation. Plant Systematics and Evolution 298(7), 1407-14. Leucanthemum. ... The base of the head is layered with up to 60 or more rough-edged phyllaries. The Leucanthemum head has about 13 to 34 ray ... Some leaves are borne on petioles, and others are sessile, attached to the stem at their bases. They vary in shape, and some ...

ProbCons

... is an open source probabilistic consistency-based multiple alignment of amino acid sequences. It is an efficient ... Probabilistic Consistency-based Multiple Sequence Alignment". Genome Research. 15 (2): 330-340. doi:10.1101/gr.2821705. PMC ... All pairs of sequences x,y from the set of all sequences S {\displaystyle {\mathcal {S}}} are now re-estimated using all ... Calculate expected accuracy of each sequence: E P r [ a , x , y ] ( a c c ( a ∗ , a ) ) = ∑ a P r [ a , x , y ] a c c ( a ∗ , a ...

Cassytha

Angiosperm phylogeny based on matK sequence information. American Journal of Botany 90: 1758-1776. Rohwer JG & Rudolph B. 2005 ... based on different analyses of trnK intron sequences. Annals of the Missouri Botanical Garden 92: 153-178. Kimoto Y, Utami N, ... Based on the trnK intron, a common phylogenetic marker for classifying angiosperms, Rohwer and Rudolph (2005) created a ... Most early systems of classification based on morphological characters divided the Lauraceae into two subfamilies: ...

Carlos Osuna

Chunyang Xiao; Marc Dymetman; Claire Gardent (7-12 August 2016). "Sequence-based Structured Prediction for Semantic Parsing". ... BizTalk is message-based system similar to TIBCO which companies integrate across realms. The server is coded using .NET but ... This proved challenging as was one of the first uses of FTP based Web Services in a SOAP-like protocol of messages and ... After 4 years of successful implementations using the JWS pattern exclusively based on Server Side Includes and JSP, Carlos ...

Maskray

Naylor G J P, Caira J N, Jensen K, Rosana K A M, White W T, Last P R (2012): A DNA sequence-based approach to the ... based on DNA sequences. Chinese Journal of Oceanology and Limnology, doi:10.1007/s00343-018-7056-2. Pavan-Kumar A, Kumar R, ... based on cytochrome-oxidase I gene sequences and spotting patterns. Comptes Rendus Biologies, 336 (4): 221-232.[2] Borsa, P. ( ... as well as in the CO1 and ND2 gene sequences. Within the genus Neotrygon, species can be diagnosed by their nucleotide sequence ...

Polyporus

based on molecular sequences". Mycotaxon. 82: 315-322. Krüger, D.; Petersen, R.H.; Hughes, K.W. (2006). "Molecular phylogenies ... Krüger, D.; Gargas A. (2004). "The basidiomycete genus Polyporus-an emendation based on phylogeny and putative secondary ... Polyporales, Basidiomycota) from China based on morphological and molecular data" (PDF). Mycological Progress. 13 (3): 811-817 ...

Brudevælte Lurs

The tone sequence is based on just intonation; a good player can produce a wide tonal range. The sound of the lurs is powerful ...

Nucleic acid secondary structure

The base pairing in pseudoknots is not well nested; that is, base pairs occur that "overlap" one another in sequence position. ... T and A rich sequences are more easily melted than C and G rich regions. Particular base steps are also susceptible to DNA ... Breslauer KJ, Frank R, Blöcker H, Marky LA (1986). "Predicting DNA duplex stability from the base sequence". PNAS. 83 (11): ... In RNA, thymine is replaced by uracil (U). Alternate hydrogen bonding patterns, such as the wobble base pair and Hoogsteen base ...

List of glycoside hydrolase families

A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of numerous different ... Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". Biochem. J. 316 (2): ... Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in 'clans'. As of ...

Gideon Davies

Henrissat, B.; Davies, G. (1997). "Structural and sequence-based classification of glycoside hydrolases". Current Opinion in ... Carbohydrates: A feast of structural glycobiology • Sequences and topology: Computational studies of protein-protein ...

C11orf52

The mRNA sequence is 1,140 base pairs long. There is an upstream stop codon located at nucleotides 65 - 67. The 23rd amino acid ... C11orf52 spans 155658 base pairs and is orientated on the positive strand. Gene C11orf52 has a molecular weight of 14kDa and is ... the most distantly related to Homo Sapiens C11orf52 sequence. Gene duplication first occurred approximately 324.5 million years ...

Alfonso Valencia

"Sequence-based feature prediction and annotation of proteins". Genome Biology. 10 (2): 206. doi:10.1186/gb-2009-10-2-206. PMC ... studying the evolution of protein function using sequence- and structure-based approaches. In 1994 Valencia formed the Protein ... 2011). "Exome sequencing identifies recurrent mutations of the splicing factor SF3B1 gene in chronic lymphocytic leukemia". ... 2011). "Whole-genome sequencing identifies recurrent mutations in chronic lymphocytic leukaemia". Nature. 475 (7354): 101-5. ...

Amos Bairoch

Henrissat, B; Bairoch, A (1993). "New families in the classification of glycosyl hydrolases based on amino acid sequence ... Henrissat, B; Bairoch, A (1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal. ... an MS-DOS based software package for the analysis of protein and nucleotide sequences. PC/Gene was commercialized, first by a ... While working on PC/Gene he started to develop an annotated protein sequence database which became Swiss-Prot and was first ...

Nucleic acid double helix

This is, again, due to the properties of the bases which make up the DNA sequence - a random sequence will have no preferred ... rotation of one base with respect to the other in the same base pair. Opening Shift: displacement along an axis in the base- ... while base-base association is nonetheless overall preserved (AT-rich). Periodic fracture of the base-pair stack with a break ... For each base pair, considered relative to its predecessor, there are the following base pair geometries to consider: Shear ...

CAZy

Henrissat, B. (1991). "A classification of glycosyl hydrolases based on amino acid sequence similarities". The Biochemical ... Henrissat, B.; Davies, G. (1997). "Structural and sequence-based classification of glycoside hydrolases". Current Opinion in ... As of November 2013, CAZy contains sequence information on nearly 340,000 CAZymes. The CAZy database is coupled with the ... CAZypedia online encyclopedia, which was launched in June 2009 and intended to be a wiki-based encyclopedia of CAZymes. As of ...

Glycoside hydrolase

... s may also be classified by sequence or structure based methods. Sequence-based classifications are among ... Based on three-dimensional structural similarities, the sequence-based families have been classified into 'clans' of related ... "Structural and sequence-based classification of glycoside hydrolases". Current Opinion in Structural Biology. 7 (5): 637-644. ... A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of more than 100 ...

CLIP

The method is based on linear amplification of the immunoprecipitated RNA and thereby improves the complexity of the sequencing ... The isolated RNA is converted into a cDNA library and deep sequenced using high-throughput sequencing technology. Cross-linking ... Tag-Based Next Generation Sequencing. p. 153. doi:10.1002/9783527644582.ch10. ISBN 978-3527644582. König, J; Zarnack, K; ... High-throughput sequencing is then used to generate reads containing distinct barcodes that identify the last cDNA nucleotide. ...

Flora of Western Australia

Flora Base. Retrieved 14 June 2017. "New linear systematic sequence for vascular plants". Flora Base. Retrieved 3 June 2017. ... As Bentham had never been to Australia, he based all his work on the material already collected, assisted by Ferdinand von ... FloraBase is a public access web-based database of the flora of Western Australia. It provides authoritative scientific ...

Nucleic acid thermodynamics

1986). "Predicting DNA Duplex Stability from the Base Sequence". Proc. Natl. Acad. Sci. USA. 83 (11): 3746-3750. doi:10.1073/ ... The interaction between bases on different strands depends somewhat on the neighboring bases. Instead of treating a DNA helix ... Because cytosine / guanine base-pairing is generally stronger than adenine / thymine base-pairing, the amount of cytosine and ... are formed between similar sequences and any differences between those sequences will result in a disruption of the base- ...

Dancemeditation

Opening sequence: a breath-based series of repetitive stretches. Basic practice subjects include: Breath dances: Rhythmic and ...

Carex subg. Vignea

Vignea (Cyperaceae) based on non-coding nrDNA sequence data". Systematic Botany. 31 (1): 70-82. doi:10.1600/036364406775971813 ... Vignea (Cyperaceae) based on amplified fragment length polymorphism and nrDNA data". Systematic Botany. 37 (4): 913-925. doi: ...

Pendon Museum

This sequence is based on timetables of the period. They are all modelled in 4 mm to 1 foot scale (1:76), and run on track of ... Operation consists of a sequence of trains, showing what one could have seen passing by on a summer day and night, in the mid- ... The model trains are hand built, to represent individual locomotives, carriages, and wagons as exactly possible, based on ...

Ariosa v. Sequenom

Courts these days are making policy-based decisions, untethered from any rule of law, aimed at killing patents they don't like. ... So they wanted to focus on genetic fragments containing paternally inherited sequences the mother did not share, but had ... mc2 and based on a requirement to keep speculator trolls out: [I]f the breadth of the claim is sufficiently limited to a ...

Vistula

The Vistula estuary was settled by Slavs in the 7th and 8th century.[26] Based on archeological and linguistic findings, it has ... "Record of the Vistula ice lobe advances in the Late Weichselian glacial sequence in north-central Poland". Quaternary ... and considerable predisposition of its older base. The asymmetry of the river basin (right-hand to left-hand side) is 73-27%. ...

Ketone

... base added to ketone, using weak or insoluble bases, e.g., NaOEt in EtOH, or NaH) provides the more-substituted thermodynamic ... Ozonolysis, and related dihydroxylation/oxidative sequences, cleave alkenes to give aldehydes or ketones, depending on alkene ... Acid/base properties of ketonesEdit. Ketones are far more acidic (pKa ≈ 20) than a regular alkane (pKa ≈ 50). This difference ... with either stoichiometric and catalytic base. Using very strong bases like lithium diisopropylamide (LDA, pKa of conjugate ...

Hymenoscyphus fraxineus

... based on morphological and DNA sequence comparisons, Chalara fraxinea was suggested to be the asexual stage (anamorph) of the ... The sequence has been published on the website OpenAshDieBack and offers clues to how the fungus infects trees. The study has ... Teams from The Sainsbury Laboratory (TSL) and the John Innes Centre in Norwich sequenced the genome of the fungus in December ... In December 2016, writing in Nature Dr Richard Buggs reported that the common ash (Fraxinus excelsior)had been sequenced for ...

Mary Astor

She then appeared in some movie shorts with sequences based on famous paintings. She received critical recognition for the 1921 ... On January 2, 1930, while filming sequences for the Fox movie Such Men Are Dangerous, Kenneth Hawks was killed in a mid-air ... but her small part in a dream sequence wound up on the cutting room floor. Paramount let her contract lapse. ...

LMNA - 维基百科，自由的百科全书

Lamin A/C gene and a related sequence map to human chromosomes 1q12.1-q23 and 10. Somat. Cell Mol. Genet. March 1993, 19 (2): ... PDB rendering based on 1ifr.. 有效结构. PDB. 直系同源检索：PDBe, RCSB ...

Quantitative trait locus

Family-pedigree based mapping[edit]. Family based QTL mapping, or Family-pedigree based mapping (Linkage and association ... Once a region of DNA is identified as contributing to a phenotype, it can be sequenced. The DNA sequence of any genes in this ... This can be done using BLAST, an online tool that allows users to enter a primary sequence and search for similar sequences ... Using family-pedigree based approach has been discussed (Bink et al. 2008). Family-based linkage and association has been ...

Clastic rock

The two major types, are silicate based and carbonate based. The majority of silica cements are composed of quartz but can ... Thick sequences of sedimentary (colluvial) breccias are generally formed next to fault scarps in grabens. ... Classification schemes for mudrocks tend to vary but most are based on the grain size of the major constituents. In mudrocks, ... However, others have used the term shale to further divide mudrocks based on the percentage of clay constituents. The plate- ...

QIAGEN Silicon Valley

... (formerly known as Ingenuity Systems, Inc) is a company based in Redwood City, California, USA, to ... Laboratory Corporation and Quest Diagnostics to develop a solution for scoring genetic variation for next generation sequencing ... All QIAGEN Silicon Valley use the Ingenuity Knowledge Base, which contains biological and chemical interactions and functional ... BD Cell Pathways "Ingenuity Systems Announces Immediate Availability Of The Ingenuity Pathways Knowledge Base" (Press release ...

Eurasian magpie

Rossini's opera La gazza ladra and The Adventures of Tintin comic The Castafiore Emerald are based on this theme. However, one ... An analysis of mitochondrial DNA sequences published in 2003 confirmed that the black-billed magpie and the yellow-billed ... Union decided to treat the black-billed magpie as a separate species based on studies of the vocalization and behaviour that ...

Divisibility rule

Minimum magnitude sequence. (1, 4, 4, 4, 4, and 4 goes on for the rest) -- Positive sequence. Multiply the right most digit by ... For example, in base 10, the factors of 101 include 2, 5, and 10. Therefore, divisibility by 2, 5, and 10 only depend on ... Positive sequence Multiply the right most digit by the left most digit in the sequence and multiply the second right most digit ... Minimum magnitude sequence (1, 3, 2, 6, 4, 5, cycle repeats for the next six digits) Period: 6 digits. Recurring numbers: 1, 3 ...

Clave (rhythm)

... the attack-point sequence of the two measures is reversed. Most salsa is in 2-3 clave and most salsa piano guajeos are based on ... The following guajeo is based on the clave motif in a 2-3 sequence. The cinquillo rhythm is now in the second measure. ... 8 clave-based music is generated from cross-rhythm, it is possible to count or feel the 6. 8 clave in several different ways. ... The following afrobeat guitar part is a variant of the 2-3 onbeat/offbeat motif.[83] Even the melodic contour is guajeo-based. ...

Streptococcus pneumoniae

The genome of S. pneumoniae is a closed, circular DNA structure that contains between 2.0 and 2.1 million base pairs depending ... The recent advances in next-generation sequencing and comparative genomics have enabled the development of robust and reliable ... S. pneumoniae can also be distinguished based on its sensitivity to lysis by bile, the so-called "bile solubility test". The ... For instance, the Xisco gene was recently described as a biomarker for PCR-based detection of S. pneumoniae and differentiation ...

Bengali language

What is accepted as the standard form today in both West Bengal and Bangladesh is based on the West-Central dialect of Nadia ... There is yet to be a uniform standard collating sequence (sorting order of graphemes to be used in dictionaries, indices, ... The modern literary form of Bengali was developed during the 19th and early 20th centuries based on the dialect spoken in the ... In spite of some modifications in the 19th century, the Bengali spelling system continues to be based on the one used for ...

Group 3 element

Pyykkö, Pekka (2011). "A suggested periodic table up to Z ≤ 172, based on Dirac-Fock calculations on atoms and ions". Physical ... to this debate on the basis of moving to a 32-column table and consideration of which option results in a continuous sequence ... Exclusion of all elements is based on properties of earlier actinides, which show a much wider variety of chemistry (for ...

Positron emission tomography

Statistical, likelihood-based approaches: Statistical, likelihood-based [37][38] iterative expectation-maximization algorithms ... Because the two scans can be performed in immediate sequence during the same session, with the patient not changing position ... based regularization in a wavelet or other domain), such as via Ulf Grenander's Sieve estimator[41][42] or via Bayes penalty ... Neurology: PET neuroimaging is based on an assumption that areas of high radioactivity are associated with brain activity. What ...

Leaf

More than one main vein (nerve) at the base. Lateral secondary veins branching from a point above the base of the leaf. Usually ... There is a regularity in these angles and they follow the numbers in a Fibonacci sequence: 1/2, 2/3, 3/5, 5/8, 8/13, 13/21, 21/ ... BaseEdit. Acuminate. Coming to a sharp, narrow, prolonged point.. Acute. Coming to a sharp, but not prolonged point.. ... When the leaf base completely surrounds the stem, the leaves are said to be perfoliate, such as in Eupatorium perfoliatum. ...

Ebola

Genome-sequencing showed that this outbreak was not related to the 2014-15 West Africa Ebola virus outbreak, but was the same ... Developments in organ-on-a-chip technology have led to a chip-based model for Ebola haemorrhagic syndrome.[262] ... Genome sequencing suggests that this outbreak, the 11th outbreak since the virus was first discovered in the country in 1976, ... December 1999). "Identification of Ebola virus sequences present as RNA or DNA in organs of terrestrial small mammals of the ...

Cushing's disease

The clinical diagnosis must be based on the presence of one or more of the symptoms listed below, because the syndrome itself ... The CRH test uses recombinant human or bovine-sequence CRH, which is administered via a 100μg intravenous bolus dose. The ... A population-based study". Journal of Clinical Endocrinology and Metabolism. 86 (1): 117-123. doi:10.1210/jcem.86.1.7093. PMID ... Aidan Carney and based on statistical evidence, was that the basophil adenoma Minnie might have harbored underwent partial ...

Oligosaccharyltransferase

... residue in the sequence Asn-X-Ser or Asn-X-Thr where X is any amino acid except proline. This sequence is called a ... Marquardt T, Denecke J (June 2003). "Congenital disorders of glycosylation: review of their molecular bases, clinical ... The high sequence similarity between the prokaryotic and the eukaryotic STT3 suggests that their structures are similar. ...

I Parachute Battery (Bull's Troop) Royal Horse Artillery

Henceforth, batteries were designated in a single alphabetical sequence in order of seniority from date of formation[24] and ... it has been based in England, initially at Aldershot but latterly at Colchester.[61] ... currently based in Merville Barracks in Colchester. ...

Medicaid

A sequence of two high-profile studies by a team from the Massachusetts Institute of Technology and the Harvard School of ... Similarly, if a child lives with someone other than a parent, he may still be eligible based on its individual status.[74] ... Unlike Medicare, Medicaid is a means-tested, needs-based social welfare or social protection program rather than a social ... The Low-Income Wage Rate: State welfare programs base the level of assistance they provide on some concept of what is minimally ...

Mallard

The mallard is omnivorous and very flexible in its choice of food.[61] Its diet may vary based on several factors, including ... Mitochondrial DNA data for the D-loop sequence suggests that mallards may have evolved in the general area of Siberia. Mallard ... The genome of Anas platyrhynchos was sequenced in 2013.[6] ...

Paleolithic

"Sex-Based Roles Gave Modern Humans an Edge, Study Says". National Geographic News. Retrieved 2008-02-03.. ... "Callaway, Ewen (22 September 2011), "First Aboriginal genome sequenced", Nature, Nature News, doi:10.1038/news.2011.551 ... An artist's rendering of a temporary wood house, based on evidence found at Terra Amata (in Nice, France) and dated to the ... In general, their actual diet in the wild is about 95% plant-based, with the remaining 5% filled with insects, eggs, and baby ...

Chloroplast DNA

Over time, base changes in the DNA sequence can arise from deamination mutations. When adenine is deaminated, it becomes ... Hypoxanthine can bind to cytosine, and when the XC base pair is replicated, it becomes a GC (thus, an A → G base change).[20] ... Its existence was first proven in 1962,[4] and first sequenced in 1986-when two Japanese research teams sequenced the ... both very common in chloroplast transit sequences-making up 20-30% of the sequence)[45] are often the amino acids that accept ...

Acne

... vulgaris is diagnosed based on a medical professional's clinical judgment.[15] The evaluation of a person with suspected ... scientists reported the first genome sequencing of a C. acnes bacteriophage (PA6). The authors proposed applying this research ... June 2012). "Topical antimicrobial treatment of acne vulgaris: an evidence-based review". American Journal of Clinical ... Levy LL, Zeichner JA (October 2012). "Management of acne scarring, part II: a comparative review of non-laser-based, minimally ...

Template:United States Postal Service

Flats Sequencing System. *Locatable Address Conversion System. *United States Postal Savings System ... Information-Based Indicia. *The Inspectors. *Intelligent Mail barcode. *Label 228. *List of postal killings ...

Substance P

Huston JP, Hasenöhrl RU, Boix F, Gerhardt P, Schwarting RK (1993). "Sequence-specific effects of neurokinin substance P on ... the peptide based NK1RA) against cisplatin-induced emesis in the ferret.[75] It is likely that some peripheral exposure ... The deduced amino acid sequence of substance P is as follows:[3] ...

Hematopoietic stem cell transplantation

... mismatch as small as a single DNA base pair is significant so perfect matches require knowledge of the exact DNA sequence of ... One study based on a survey of medical teams covered approximately 24,000 peripheral blood HSCT cases between 1993 and 2005, ...

Glossary of graph theory terms

... a vertex sequence such that each edge goes from an earlier vertex to a later vertex in the sequence.. totally disconnected. ... based on vertex removals.. strong. 1. For strong connectivity and strongly connected components of directed graphs, see ... The degree sequence is the collection of degrees of all vertices, in sorted order from largest to smallest. In a directed graph ... A path may either be a walk (a sequence of vertices and edges, with both endpoints of an edge appearing adjacent to it in the ...