In the fungal mitochondrial ribosomes, 5S rRNA is replaced by LSU rRNA expansion sequences. In kinetoplastids (euglenozoans), ... "The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia coli 50 S ribosomal subunit based on a cryo-electron microscopic ... 5S rRNAs were only identified with specialized covariance models that incorporate information on the pronounced sequence ...

In RNA, which also has extensive secondary structure, the linear chain of bases is generally just referred to as the "sequence ... Peptides can be directly sequenced, or inferred from DNA sequences. Large sequence databases now exist that collate known ... Sequence families are often determined by sequence clustering, and structural genomics projects aim to produce a set of ... Although it does not change the sequence, it does affect the chemical properties of the sequence. In particular, the L-amino ...

The idea arose that different DNA sequences could be inserted into a plasmid and that these foreign sequences would be carried ... If the DNA to be cloned is exceptionally large (hundreds of thousands to millions of base pairs), then a bacterial artificial ... vector DNA with no recombinant sequence inserted). In these vectors, foreign DNA is inserted into a sequence that encodes an ... Virtually any DNA sequence can be cloned and amplified, but there are some factors that might limit the success of the process ...

... so if the original codon on the DNA sequence was C-U-A and there was a small mutation in the DNA sequence that led to a C-U-U ... base on the mRNA, was not as spatially confined as the other two bases, and could, thus, have non-standard base pairing. Crick ... The thermodynamic stability of a wobble base pair is comparable to that of a Watson-Crick base pair. Wobble base pairs are ... A wobble base pair is a pairing between two nucleotides in RNA molecules that does not follow Watson-Crick base pair rules. The ...

Base sequence of the recognition site". Journal of Molecular Biology. 51 (2): 393-409. doi:10.1016/0022-2836(70)90150-6. PMID ... Kelly determined the DNA sequences recognized by type II restriction enzymes, which subsequently became major tools in ...

The base pairing in pseudoknots is not well nested; that is, base pairs occur that "overlap" one another in sequence position. ... T and A rich sequences are more easily melted than C and G rich regions. Particular base steps are also susceptible to DNA ... Breslauer KJ, Frank R, Blöcker H, Marky LA (1986). "Predicting DNA duplex stability from the base sequence". PNAS. 83 (11): ... In RNA, thymine is replaced by uracil (U). Alternate hydrogen bonding patterns, such as the wobble base pair and Hoogsteen base ...

2003). "End-sequence profiling: Sequence-based analysis of aberrant genomes". Proceedings of the National Academy of Sciences ... Influence of neighbouring base sequences on mutagenesis". Biochimica et Biophysica Acta. 1171 (1): 11-18. doi:10.1016/0167-4781 ... Genomics technologies include: DNA sequencing: Pyrosequencing-based sequencers offer a relatively low-cost method to generate ... Bacterial Artificial Chromosome (BAC)-end sequencing (end-sequence profiling): Identifies chromosomal breakpoints by generating ...

1986). "Predicting DNA Duplex Stability from the Base Sequence". Proc. Natl. Acad. Sci. USA. 83 (11): 3746-3750. doi:10.1073/ ... The interaction between bases on different strands depends somewhat on the neighboring bases. Instead of treating a DNA helix ... Because cytosine / guanine base-pairing is generally stronger than adenine / thymine base-pairing, the amount of cytosine and ... are formed between similar sequences and any differences between those sequences will result in a disruption of the base- ...

Gene K overlaps genes A, B, and C. The origin of replication lies within a 30 base sequence. The entire 30 base sequence is ... Viruses are assigned according to their similarity to known lab based strains-the ΦX174-like clade, G4-like clade and the α3- ... Zsak L, Day JM, Oakley BB, Seal BS (2011) The complete genome sequence and genetic analysis of ΦCA82 a novel uncultured ... Kodaira K, Nakano K, Okada S, Taketo A (1992) Nucleotide sequence of the genome of the bacteriophage alpha 3: interrelationship ...

At any location within such a sequence, the bases found in a given position may vary between organisms. The particular sequence ... These have been replaced in recent times largely by DNA sequencing, which produces the exact sequences of nucleotides or bases ... The base sequences for the haplotypes are then compared. In the simplest case, the difference between two haplotypes is ... The advantage claimed for using hybridisation rather than gene sequencing was that it was based on the entire genotype, rather ...

Bishoff-Grethe, A.; Goedert, K.M.; Willingham, D.T.; Grafton, S.T. (2004). "Neural substrates of response-based sequence ... Bishoff-Grethe, A.; Goedert, K.M.; Willingham, D.T.; Grafton, S.T. (2004). "Neural substrates of response-based sequence ... "The spatial representation of numerical and non-numerical sequences: Evidence from neglect". Neruopsychologia. 44 (7): 1061- ... "The spatial representation of numerical and non-numerical sequences: Evidence from neglect". Neruopsychologia. 44 (7): 1061- ...

1978 - Frederick Sanger presented the 5,386 base sequence for the virus PhiX174; first sequencing of an entire genome. 1982 - ... 1977 - Walter Gilbert and Allan Maxam present a rapid DNA sequencing technique which uses cloning, base destroying chemicals, ... Fred Sanger used radioactive phosphorus as a tracer to chromatographically decipher a 120 base long RNA sequence. 1969 - ... 1951 - Fred Sanger, Hans Tuppy, and Ted Thompson completed their chromatographic analysis of the insulin amino acid sequence. ...

This is, again, due to the properties of the bases which make up the DNA sequence - a random sequence will have no preferred ... rotation of one base with respect to the other in the same base pair. Opening Shift: displacement along an axis in the base- ... while base-base association is nonetheless overall preserved (AT-rich). Periodic fracture of the base-pair stack with a break ... For each base pair, considered relative to its predecessor, there are the following base pair geometries to consider: Shear ...

... www.genomeweb.com/sequencing/dnanexus-aims-alleviate-next-gen-sequence-analysis-headache-cloud-based-service Ricketts, Camille ... DNAnexus is an American company that provides a cloud-based data analysis and management platform for DNA sequence data. It is ... In April 2010, DNAnexus launched its cloud-based sequence data service. In August 2011, the company expanded its recruiting ... "DNAnexus Aims to Alleviate Next-Gen Sequence Analysis Headache with Cloud-based Service." In Sequence, April 20, 2010. http:// ...

Universal bases may pair indiscriminately with any other base, but, in general, lower the melting temperature of the sequence ... which may pair with canonical bases, resulting in four possible base-pairs (8 bases:xA-T,xT-A,xC-G,xG-C, 16 bases if the unused ... base pair. One of the most common base analogs is 5-bromouracil (5BU), the abnormal base found in the mutagenic nucleotide ... different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical ...

Its ability to recognise double-stranded nucleic acids depends on the base sequence. It tends to cleave at ApN and at T(U) pN. ... Unidirectional deletion of large DNA (in combination with Exonuclease III) to generate ordered deletions for sequencing. ... Excision of gene coding sequences from genomic DNA. Molecular and Cellular Biology portal. ...

Structure-based sequence alignment and site-directed mutagenesis identified the putative PD..D/EXK active sites of the EcoRII ... EcoRII is a bacterial Type IIE REase that interacts with two or three copies of the pseudopalindromic DNA recognition sequence ... EcoRII cut target DNA sequence CCWGG generating sticky ends. The apo crystal structure of EcoRII mutant R88A (PDB: 1NA6​) has ...

The DGGE gel will separate genes of the same size based on base sequence. This technique shows to what extent microbial ... is a microbial fingerprinting technique that separates amplicons of roughly the same size based on sequence properties (Figure ... A GC clamp (about 40 bases with high GC content) is used as a special primer to anchor the PCR fragments together once they ... This method does not require that the microbes be cultured in the lab and does not require any sequence data needed to design ...

Each tRNA contains a distinct anticodon triplet sequence that can base-pair to one or more codons for an amino acid. Some ... the CCA sequence is transcribed in some tRNA sequences. In most prokaryotic tRNAs and eukaryotic tRNAs, the CCA sequence is ... Felsenfeld G, Cantoni G; Cantoni (1964). "Use of thermal denaturation studies to investigate the base sequence of yeast serine ... These unusual bases sometimes affect the tRNA's interaction with ribosomes and sometimes occur in the anticodon to alter base- ...

Usually 25 bases in length, they bind to complementary sequences of RNA or single-stranded DNA by standard nucleic acid base- ... Morpholinos block access of other molecules to small (~25 base) specific sequences of the base-pairing surfaces of ribonucleic ... A Morpholino can modify splicing or block translation, depending on the Morpholino's base sequence. Bound to the 5'- ... encoded as a sequence of bases in DNA, is converted into the structure of a protein. ...

... a big data toolset for family based sequencing data analysis". Journal of Medical Genetics. 52: 282-8. doi:10.1136/jmedgenet- ... This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other ... Dikstein R, Ruppert S, Tjian R (March 1996). "TAFII250 is a bipartite protein kinase that phosphorylates the base transcription ... "X-exome sequencing of 405 unresolved families identifies seven novel intellectual disability genes". Molecular Psychiatry. 21: ...

Lee, S.T.; Kim, J.; Kang, H.S. (1984). "A computer program for searching for tRNA gene from base sequences of DNA". Korean ... He developed a program to fold tRNA sequences published in the Korean Journal of Biochemistry. This paper is now known as the ... without using primary sequence homology, which led to the cloning of olfactory receptors in insects. After his move to Penn, ...

Some taxonomists have lumped the S. sanguinis group in with the S. mitis group based on 16S rRNA gene sequence analysis, but S ... sequence homology in this gene. In light of the high degree of 16S rRNA gene sequence similarity, sequencing of alternative ... Other sequence-based identification systems have subsequently been introduced for VGS species level identification. In general ... Sequence-based identification. Historically, DNA-DNA hybridization studies have been used to confirm species level ...

"Gene-based sequence-tagged-sites (STSs) as the basis for a human gene map". Nat Genet. 10 (4): 415-23. doi:10.1038/ng0895-415. ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...

"Gene-based sequence-tagged-sites (STSs) as the basis for a human gene map". Nat. Genet. 10 (4): 415-23. doi:10.1038/ng0895-415 ...

The viral genetic material is contained in double stranded linear DNA consisting of 202,182 tightly packed base pairs. The DNA ... Gubser, Caroline; Smith, Geoffrey (2001). "The sequence of Camelpox virus shows it is most closely related to Variola virus, ... Camelpox diagnosis can be based on symptoms. However, both Camel Contagious Ecthyma and Camel Papillomatosis cause ...

These codes make it easier and shorter to write down the amino acid sequences that make up proteins. The nucleotide bases are ... Some important work IUPAC has done in these fields includes standardizing nucleotide base sequence code names; publishing books ... IUPAC needed a coding system that represented long sequences of amino acids. This would allow for these sequences to be ... These nucleotide base codes make the genome of an organism much smaller and easier to read. The codes for amino acids (24 amino ...