Job Zebrafish Research Technician - School of Dental Medicine - Jobs for Zebrafish Research Technician - School of Dental...
Job Zebrafish Research Technician - School of Dental Medicine on Resumark.com. Search jobs like Zebrafish Research Technician - School of Dental Medicine in Boston, Massachusetts - Education & Science / Technician / Mechanic and express interest in any job.http://www.resumark.com/job/job-Zebrafish-Research-Technician-School-of-Dental-Medicine-BH34B7FRF
Transcriptome dynamics in early zebrafish embryogenesis determined by high-resolution time course analysis of 180 successive,...
In this study we set out to investigate the transcriptome dynamics of early zebrafish embryogenesis with a comprehensive experimental set-up that interrogahttps://redhothealthcare.com/transcriptome-dynamics-in-early-zebrafish-embryogenesis-determined-by-high-resolution-time-course-analysis-of-180-successive-individual-zebrafish-embryos/
Lirias: The effect of 3,5,3'-triiodothyronine supplementation on zebrafish (Danio rerio) embryonic development and expression...
The importance of thyroid hormones (TH) for embryonic development has long been shown in many vertebrates, but is not yet established in pre-hatch teleost models despite the presence of TH, TH receptors and iodothyronine deiodinases. Lack of data about the dynamics of TH metabolism in embryonic stages of fish does not allow to speculate about the involvement and/or role of TH in fish embryonic development. We therefore set up an experiment to examine the effect of 3,5,3'-triiodothyronine (T(3)) on zebrafish (Danio rerio) embryonic development and on the expression of some thyroid hormone-regulated genes as measured by real-time PCR. Maternally derived thyroxine (T(4)) and T(3) were detected throughout embryonic development and total levels remained stable. Thyroid hormone receptor (TR) alpha and beta mRNA were found to be present in early embryos. After an initial fall, TRalpha mRNA levels in the control group increased gradually from 12h post fertilization (HPF) onwards. TRbeta mRNA levels ...https://lirias.kuleuven.be/handle/123456789/69808
Cell cycle analysis of transgenic zebrafish embryos exp | Open-i
Cell cycle analysis of transgenic zebrafish embryos expressing PCNA-CB. (A) Overview of the dorsal midbrain of a wnt1:gal4,UAS:GFP (green); UAS:PCNA-CB (magentahttps://openi.nlm.nih.gov/detailedresult.php?img=PMC4440926_develop-142-118943-g3&req=4
AID 620365 - Antiangiogenic activity in fli-1:enhanced GFP expressing transgenic zebrafish embryo assessed as inhibition of...
BioAssay record AID 620365 submitted by ChEMBL: Antiangiogenic activity in fli-1:enhanced GFP expressing transgenic zebrafish embryo assessed as inhibition of neovacularisation at 5 uM after 24 hrs relative to control.https://pubchem.ncbi.nlm.nih.gov/bioassay/620365
Zebrafish Mutation Project: Functional Genomics of Disease.
The advent of high-throughput sequencing has greatly accelerated the identification of inherited and de novo disease causing mutations. Following discovery the analysis of the developmental and cellular pathways of the affected genes is a crucial step on the path towards therapy with model organisms as the central tool. Traditionally, vertebrate model organisms such as mouse and zebrafish have been used on a gene-by-gene basis, however, in order to keep pace with the increasing speed of discovery, new approaches are needed. Owing to a high quality genome reference sequence and its genetic and embryological tractability the zebrafish is a vertebrate model especially suited for large scale studies. Previously having established methods to generate and identify disruptive zebrafish point mutations on a genome wide scale the Zebrafish Mutation Project is now assigning biological function to every protein-coding gene in the ...http://www.ashg.org/2013meeting/abstracts/fulltext/f130121952.htm
Effect of starvation on transcriptomes of brain and liver in adult female zebrafish (Danio rerio). - Semantic Scholar
We used microarray and quantitative real-time PCR (qRT-PCR) analyses in adult female zebrafish (Danio rerio) to identify metabolic pathways regulated by starvation in the liver and brain. The transcriptome of whole zebrafish brain showed little response to 21 days of starvation. Only agouti-related protein 1 (agrp1) significantly responded, with increased expression in brains of starved fish. In contrast, a 21-day period of starvation significantly downregulated 466 and upregulated 108 transcripts in the liver, indicating an overall decrease in metabolic activity, reduced lipid metabolism, protein biosynthesis, proteolysis, and cellular respiration, and increased gluconeogenesis. Starvation also regulated expression of many components of the unfolded protein response, the first such report in a species other than yeast (Saccharomyces cerevisiae) and mice (Mus musculus). The response of the zebrafish hepatic transcriptome to starvation was strikingly similar ...https://www.semanticscholar.org/paper/Effect-of-starvation-on-transcriptomes-of-brain-an-Drew-Rodnick/04198363c4a8a0dcbcaab088caa199ef5069f1ad
Danio rerio (Zebrafish) (Brachydanio rerio)
www.cas.vanderbilt.edu. Danio rerio commonly known as zebrafish is a tropical fish belonging to the minnow family (Cyprinidae). It is a major model for studying developmental biology; aided by the transparency of the embryo and adult body, researchers can modify the fish's genotype at the egg stage and see resulting changes as little as three days later.. Zebrafish have the ability to regenerate many of their tissues including fins, skin, heart and, in larval stages, brain. Zebrafish have been used to study cancers, including melanoma, leukemia, pancreatic cancer and hepatocellular carcinoma. The Tubingen strain was used as the the reference strain for the genome sequencing project as it had been used extensively to identify mutations affecting embryogenesis. Sequencing revealed that 71.4% of human genes have at least one zebrafish ortholog. Currently the zebrafish genome is thought to have more than 26,000 protein-coding ...http://www.uniprot.org/proteomes/UP000000437
Antiangiogenic Cancer Drug Using the Zebrafish Model | Arteriosclerosis, Thrombosis, and Vascular Biology
Because of its permeability to small molecules, zebrafish can be used for testing and screening of drugs affecting different biological processes.50-52 Single and multiple compounds tests can be easily and successfully achieved on both zebrafish embryos and adults.53,54 The reason for this resides in the fact that (1) a high quantity of zebrafish embryos (≈5000) can be obtained synchronously, and (2) the small size of the embryos allows them to fit in a 384-well plate and thus allows high-throughput analyses. In this way, libraries of thousands of compounds can be screened for their effects in a reasonable time frame (eg, weeks). Progresses for drug screening in zebrafish embryos are also subjected to automatic readout for phenotypic effects. An automated high-throughput platform for in vivo chemical screenings on zebrafish embryos has been developed aiming for the highest possible throughput and minimization of human error. ...http://atvb.ahajournals.org/content/34/9/1846
Circadian rhythm of electroretinograms in living zebrafish larvae | IOVS | ARVO Journals
Purpose: Emran et al (2010) reported that the amplitudes of the electroretinograms (ERGs) recorded from isolated eyes of larval zebrafish were normal throughout the day, but were almost absent after several hours of darkness at night. The purpose of our study was to confirm this circadian rhythm of the ERGs in living zebrafish.. Methods: Initially, zebrafish (Danio rerio) were kept in a 14:10 light:dark cycle (room fluorescent light, 9:00 to 23 hours). ERGs were elicited by stimulus intensities of 790 mW/m2 and durations of 1000 msec. The ERGs were recorded with an Ag/AgCl wire electrode that was inserted into a glass micropipette filled with E3 medium. A glasspipette electrode was positioned in the center of the cornea and a reference electrode was an Ag/AgCl pellet that placed beneath the larvae body. The zebrafish was anesthetized by 3-aminobenzoic acid methylester and placed on its side on a moisture sponge with one eye facing toward a ...http://iovs.arvojournals.org/article.aspx?articleid=2148199
ZFIN Publication: Lewis et al., 2004
We provide the first analysis of how a segmentally reiterated pattern of neurons is specified along the anteroposterior axis of the vertebrate spinal cord by investigating how zebrafish primary motoneurons are patterned. Two identified primary motoneuron subtypes, MiP and CaP, occupy distinct locations within the ventral neural tube relative to overlying somites, express different genes and innervate different muscle territories. In all vertebrates examined so far, paraxial mesoderm-derived signals specify distinct motoneuron subpopulations in specific anteroposterior regions of the spinal cord. We show that signals from paraxial mesoderm also control the much finer-grained segmental patterning of zebrafish primary motoneurons. We examined primary motoneuron specification in several zebrafish mutants that have distinct effects on paraxial mesoderm development. Our findings suggest that in the absence of signals from paraxial mesoderm, primary motoneurons ...http://zfin.org/ZDB-PUB-040216-18
PLOS Genetics: Transcriptome Analysis of Zebrafish Embryogenesis Using Microarrays
Zebrafish (Danio rerio) is a well-recognized model for the study of vertebrate developmental genetics, yet at the same time little is known about the transcriptional events that underlie zebrafish embryogenesis. Here we have employed microarray analysis to study the temporal activity of developmentally regulated genes during zebrafish embryogenesis. Transcriptome analysis at 12 different embryonic time points covering five different developmental stages (maternal, blastula, gastrula, segmentation, and pharyngula) revealed a highly dynamic transcriptional profile. Hierarchical clustering, stage-specific clustering, and algorithms to detect onset and peak of gene expression revealed clearly demarcated transcript clusters with maximum gene activity at distinct developmental stages as well as co-regulated expression of gene groups involved in dedicated functions such as organogenesis. Our study also revealed a previously unidentified cohort of genes that are ...http://journals.plos.org/plosgenetics/article/related?id=10.1371/journal.pgen.0010029
Calcium modulation and dynamics in adult zebrafish cone photoreceptors | IOVS | ARVO Journals
Purpose: Ca2+ regulates a variety of cellular processes including signal transduction, energy production, and mitochondrial function. Because photoreceptors (PRs) use significant quantities of energy, consuming more ATP/minute per gram tissue than even the brain, our goal is to understand how PRs respond to external fluctuations of Ca2+, and how intracellular Ca2+ dynamics influence energy metabolism. We have previously shown that larval zebrafish PRs display heterogeneous Ca2+ transients. In this study we investigate Ca2+ dynamics in adult zebrafish PRs.. Methods: Adult zebrafish retinas expressing the genetically encoded fluorescent Ca2+ sensor GCaMP in cone PRs were dissected, flat-mounted, and sliced into 400-µm sections. Retinas were stained with propidium iodide or BODIPY and slices were imaged in a temperature-controlled chamber. Retinas were perfused with 0 mM or 3 mM Ca2+ Ringer's solution with and without 10 mM isridipine. GCaMP fluorescence was ...http://iovs.arvojournals.org/article.aspx?articleid=2335551
The genomic organization of the mammalian Pcdh and Ugt1 gene clusters is strikingly similar in that both contain a tandem array of highly similar variable exons followed by a common set of downstream constant exons (Zhang et al., 2004). In contrast to the cell-specific expression of Pcdh genes in the brain, members of the mouse Ugt1 cluster display tissue-specific expression patterns in a wide variety of tissues, especially in the digestive and respiratory systems (Zhang et al., 2004). In addition, the variable and constant genomic organizations of both the Pcdh and Ugt1 clusters are conserved in zebrafish (Wu, 2005; Li and Wu, 2007). The zebrafish has become an important vertebrate model organism for developmental biology, physiology, and pathology studies (Howe et al., 2013). Recently, there has been great interest in developing the zebrafish as a vertebrate model organism for drug discovery as well as for environmental toxicology (Hill et al., 2005; ...http://molpharm.aspetjournals.org/content/86/1/62
Double olfm1a and olfm1b knockout in zebrafish causes moderate abnormality in retinal development and function | IOVS | ARVO...
Purpose: The olfm1 gene encodes a secreted glycoprotein highly conserved in vertebrates. There are two olfm1 genes in zebrafish, olfm1a and olfm1b. Both of these genes are expressed in the brain and retina starting from 16 h post fertilization to adults. We generated a null mutant of both olfm1a and olfm1b genes and analyzed its retinal structure and visual function.. Methods: Olfm1a and olfm1b mutant alleles with nonsense point mutations were obtained from the Wellcome Trust Sanger Institute. Olfm1a and olfm1b null mutants were bred to generate double null mutant (olfm1a/b null). Spontaneous movement, optokinetic and optomotor responses, behavioral responses to light increments and decrements and electroretinogram (ERG) to increased (ON) and decreased (OFF) illumination were compared between olfm1a/b null and wild-type larvae 7 days post fertilization (dpf). The retinal morphology was examined by immunostaining of frozen sections. Total RNA was isolated from 3 and 7 dpf larvae for RNA ...http://iovs.arvojournals.org/article.aspx?articleid=2268949
ZFIN Publication: Alexander et al., 1998
The genetic pathways underlying the induction and anterior-posterior patterning of the heart are poorly understood. The recent emergence of the zebrafish model system now allows a classical genetic approach to such challenging problems in vertebrate development. Two large-scale screens for mutations affecting zebrafish embryonic development have recently been completed; among the hundreds of mutations identified were several that affect specific aspects of cardiac morphogenesis, differentiation, and function. However, very few mutations affecting induction and/or anterior-posterior patterning of the heart were identified. We hypothesize that a directed approach utilizing molecular markers to examine these particular steps of heart development will uncover additional such mutations. To test this hypothesis, we are conducting two parallel screens for mutations that affect either the induction or the anterior-posterior patterning of the zebrafish heart. As an ...https://zfin.org/ZDB-PUB-980624-8
Zebra Danio, Brachydanio rerio Profile, with care, maintenance requirements and breeding information for your tropical fish
Had gathered much info from this site, so thought I should contribute my experience. I'm new to breeding Danios, all by accident really. Within a month of setting up my 5ft tank, I had very busy zebra danio's chasing each other. A few days later I did a gravel vacuum, and emptied the water into the bath, letting it go down the drain. On the 4th bucket I decided to put the plug in, and discovered babies in the bucket. (Who knows how many I sent down the drain...)We moved them to the main tank, in a floating fry tank covered with an ankle stocking around it for finer holes. Left this to float on the top. Discovered that the water surface doesn't get agitated enough in these and babies died off. When I let fresh water in the top daily, the fish were much happier. Did another gravel vac 2 weeks later, and found more fry, so suspected they'd had another batch. My Leopard Danio girl got very plump, so I moved her and her mate into a small tank for spawning one night, and the next morning she was very ...http://www.badmanstropicalfish.com/profiles/profile54.html
Int J Dev Biol - Zebrafish development and regeneration: new tools for biomedical research
Basic research in pattern formation is concerned with the generation of phenotypes and tissues. It can therefore lead to new tools for medical research. These include phenotypic screening assays, applications in tissue engineering, as well as general advances in biomedical knowledge. Our aim here is to discuss this emerging field with special reference to tools based on zebrafish developmental biology. We describe phenotypic screening assays being developed in our own and other labs. Our assays involve: (i) systemic or local administration of a test compound or drug to zebrafish in vivo; (ii) the subsequent detection or 'readout' of a defined phenotypic change. A positive readout may result from binding of the test compound to a molecular target involved in a developmental pathway. We present preliminary data on assays for compounds that modulate skeletal patterning, bone turnover, immune responses, inflammation and early-life stress. The assays use live ...http://www.ijdb.ehu.es/web/paper/082615sb/zebrafish-development-and-regeneration-new-tools-for-biomedical-research-
a. SOX10/MyoD in situ experiments on zebrafish colec11 | Open-i
a. SOX10/MyoD in situ experiments on zebrafish colec11 and masp1 morphants showing an abnormal distribution of the CNCC in the hindbrain of 10 somite-stage embrhttps://openi.nlm.nih.gov/detailedresult.php?img=PMC3045628_ukmss-33850-f0005&req=4
What zebrafish can teach us about healing brain damage | KurzweilAI
Zebrafish neurogenesis (nerve-cell creation): Left: zebrafish brain showing microglia cells. Center: lesion (damage) results in microglia activation andhttp://www.kurzweilai.net/what-zebrafish-can-teach-us-about-healing-brain-damage
AID 653187 - Toxicity in transgenic zebrafish larvae harboring Fli-1 gene assessed as maximum tolerated dosage after 24 hrs -...
BioAssay record AID 653187 submitted by ChEMBL: Toxicity in transgenic zebrafish larvae harboring Fli-1 gene assessed as maximum tolerated dosage after 24 hrs.https://pubchem.ncbi.nlm.nih.gov/bioassay/653187
Hutchinson Center Researchers First to Induce State of Suspended Animation in Model Vertebrate Organism
While such recovery from biological limbo seemingly defies scientific or medical explanation, researchers at Seattle's Fred Hutchinson Cancer Research Center have developed a method to induce a similar state of so-called suspended animation in the zebrafish, a relatively new model of vertebrate developmental biology.http://www.fredhutch.org/en/news/releases/2001/06/suspended.html
The effects of endogenous and exogenous nitric oxide on gut motility in zebrafish Danio rerio embryos and larvae | Journal of...
The early appearance of neurotransmitters in the gut seems to be a common feature to several neurotransmitters, within and between species. The first observation of detectable levels of NOS in the present study correlates well with the occurrence of other putative neurotransmitters, both excitatory (NKA, acetylcholine) and inhibitory (PACAP), in zebrafish larvae (Holmberg et al., 2004). In addition, a variety of inhibitory and excitatory neurotransmitters have been observed in other vertebrate species including mammals, amphibians and teleosts before or around the onset of exogenous feeding (Gintzler et al., 1980; Rothman and Gershon, 1982; Saffrey et al., 1982; Epstein et al., 1985; Huang et al., 1986; Saffrey and Burnstock, 1988; Reinecke et al., 1997; Salvi et al., 1999; Holmberg et al., 2001; Maake et al., 2001; Badawy and Reinecke, 2003; Holmberg et al., 2004; Pederzoli et al., 2004).. One limitation of most of the above studies is that they are strictly morphological, and functional ...http://jeb.biologists.org/content/209/13/2472
Dr. David Lyons - ENI-NET
My lab uses zebrafish as a model to study myelination in the development central nervous system. The small size, optical transparency, relative simplicity, and rapid development of zebrafish embryos are properties that allow direct observation of entire developmental (or repair) events as they occur in live animals. We have developed a non-invasive transgenic method to induce demyelination in zebrafish and have also generated a suite of tools to visualise myelin and myelinated axons at high-resolution in live zebrafish, which allows us to observe cellular, sub-cellular and molecular behaviours during myelination, demyelination, and remyelination as they occur in a living animal. Zebrafish are well established as a powerful system with which to identify new genes required for biological events. In a genetic screen carried out at Stanford University I identified new roles for genes with known involvement in myelination, ...http://www.eni-net.org/members/dr-david-lyons
Zebrafish at the Interface of Development and Disease Research, Volume 124 - 1st Edition
Purchase Zebrafish at the Interface of Development and Disease Research, Volume 124 - 1st Edition. Print Book & E-Book. ISBN 9780128033081, 9780128033432https://www.elsevier.com/books/zebrafish-at-the-interface-of-development-and-disease-research/sadler-edepli/978-0-12-803308-1