... by molecular fusion of a voltage-sensing domain operand with different fluorescent reporter proteins. These genetically encoded ... researchers in our laboratory have engineered and developed several series of genetically encoded voltage-sensitive fluorescent ... Voltage-Sensitive Dye Imaging / methods* Substances * Luminescent Proteins ... researchers in our laboratory have engineered and developed several series of genetically encoded voltage-sensitive fluorescent ...
The core material for imaging brain activity with voltage-sensitive dyes are the dyes themselves. These voltage-sensitive dyes ... Weaknesses of imaging brain activity with voltage-sensitive dyes include the following problems: Voltage-sensitive dyes may ... Recently developed fuorinated voltages sensitive dyes have been shown to mitigate these effects. Voltage-sensitive dyes have ... Voltage-sensitive dyes, also known as potentiometric dyes, are dyes which change their spectral properties in response to ...
... including voltage and calcium imaging and extracellular electrophysiological recordings with multi-electrode arrays (MEAs). ... including voltage and calcium imaging and extracellular electrophysiological recordings with multi-electrode arrays. These ... To be able to explore neuronal and microcircuit dynamics, advanced imaging, electrophysiological techniques and computational ... To be able to explore neuronal and microcircuit dynamics, advanced imaging, electrophysiological techniques and computational ...
73. Confocal Calcium Imaging of Neuronal Activity in Larval Zebrafish. Joseph R. Fetcho. 74. Voltage-Sensitive Dye Imaging of ... 75. Voltage-Sensitive Dye Imaging of Cortical Spatiotemporal Dynamics in Awake Behaving Mice. Carl C.H. Petersen. 76. Two- ... 48. Imaging with Voltage-Sensitive Dyes: Spike Signals, Population Signals, and Retrograde Transport. Bradley Baker, Xin Gao, ... 28. Imaging Calcium Waves and Sparks in Central Neurons. William N. Ross and Satoshi Manita. 29. Dendritic Voltage Imaging. ...
1986) Voltage-sensitive dyes reveal a modular organization in monkey striate cortex. Nature 321:579-585. ... Electrophysiological imaging versus optical imaging. The use of multielectrode arrays, in combination with firing-rate ... In contrast to the electrophysiological imaging applied in these experiments, optical imaging has a somewhat greater spatial ... Optical imaging (OI) offers an alternative means to assess 2D architecture. For example, optical images of orientation ...
... voltage-sensitive dye imaging in freely moving mice. Neuron 50, 617-629 (2006) ... Zhang, Y. P. & Oertner, T. G. Optical induction of synaptic plasticity using a light-sensitive channel. Nature Methods 4, 139- ...
... calcium and voltage-sensitive dye imaging, extracellular recordings of units, field potential and EEG from awake and ... optogenetics, calcium and voltage-sensitive dye imaging. *extracellular recordings of units. *field potential and EEG from ...
We demonstrate that hippocampal slices can be imaged through transparent graphene electrodes by both confocal and two-photon ... which enables simultaneous optical imaging and electrophysiological recording. ... Calcium imaging is a versatile experimental approach capable of resolving single neurons with single-cell spatial resolution in ... record high-frequency bursting activity and slow synaptic potentials that are hard to resolve by multicellular calcium imaging ...
voltage-sensitive dye imaging, intracellular calcium measurements. - microstimulation, microinjection, microsurgery. - ... in vivo calcium imaging, immunohistochemistry, confocal imaging as well as behavioral approaches. Our work is presently ... Numerous imaging techniques were utilized, including fluorescent confocal microscopy and microCT. I expanded my dissertation ... In a rodent olfactory bulb slice preparation my lab uses patch clamp electrophysiology and imaging techniques to characterize ...
Scalable Classification in Large Scale Spatiotemporal Domains Applied to Voltage-Sensitive Dye Imaging. ICDM 2009: 543-551. ... Ron Katz, Sarit Kraus: Gender-Sensitive Automated Negotiators. AAAI 2007: 821-826. ...
Ferezou I, Bolea S, Petersen CC (2006) Visualizing the cortical representation of whisker touch: voltage-sensitive dye imaging ... Takashima I, Kajiwara R, Iijima T (2001) Voltage-sensitive dye versus intrinsic signal optical imaging: comparison of optically ... Imaging cortical dynamics at high spatial and temporal resolution with novel blue voltage-sensitive dyes. Neuron 24:791-802 ... Imaging brain activity with voltage- and calcium-sensitive dyes. Cell Mol Neurobiol 25:245-282 ...
Moreover, voltage sensitive dye imaging and multi-electrode array (MEA) recordings, combined with mathematical simulations ...
Newton, T., Abdellah, M., Chevtchenko, G., Muller, E., Markram, H. Voltage-Sensitive Dye Imaging reveals inhibitory modulation ... A Kinetic Map of the Homomeric Voltage-Gated Potassium Channel (Kv) Family. Front. Cell. Neurosci., 20 August 2019. DOI: ... Pavone FS A versatile clearing agent for multi-modal brain imaging. Scientific Reports. 2015 May 7;5:9808.. DOI: 10.1038/ ... Calcium Transients in Apical Dendrites Evoked by Single Sub-Threshold Excitatory Post-Synaptic Potentials via Low Voltage- ...
Multi-electrode arrays, calcium imaging, voltage-sensitive dyes and intrinsic imaging measure mesoscale population activity. ... Voltage-sensitive dye imaging (VSDI) measures local changes in pooled membrane potentials, simultaneously from dozens of square ... voltage sensitive dye imaging (Chen and Seidemann, 2012) and single-units and EEG (McAdams and Maunsell, 1999; Williford and ... Graphics Competition: Banner Image by Karissa Payne, T-Shirt Image by Evi Hendrikx ...
A) Voltage-sensitive dye fluorescence image of the soma-axon region in ... A) Voltage-sensitive dye fluorescence image of the soma-axon region in recording position shown on the left. Traces on the ... from nine locations indicated on the image. Each trace is a spatial average of 4-5 same color pixels. Signals are scaled to the ... Voltage Sensitive Dyes. .add_svg__a{fill:none;stroke:currentColor;stroke-linecap:round;stroke-linejoin:round}. ...
... www.scholarpedia.org/article/Voltage-sensitive_dye). (5) "Invertebrates, for example, do not insulate their axons with myelin, ... not mention any alternative methods of estimating conduction velocity or latency such as membrane potential optical imaging (e. ...
Imaging hVOS signals in these slices recapitulated the tonotopic organization. Imaging with a voltage sensitive dye provided a ... We tested two approaches based on fundamentally different voltage sensing technologies. Using a voltage-sensitive dye we ... In contrast to the axonal origin of voltage-sensitive dye signals, hVOS signals represent predominantly post-synaptic responses ... Imaging Voltage in Genetically Defined Neuronal Subpopulations with a Cre Recombinase-Targeted Hybrid Voltage Sensor JOURNAL OF ...
Much of this work is grounded on data using optical imaging with voltage-sensitive dyes (VSDI). Eyal Seidemann of UT Psychology ...
"Network hyperexcitability in hippocampal slices from MECP2 mutant mice revealed by voltage-sensitive dye imaging," Journal of ... J. W. Pan, J. B. Lane, H. Hetherington, and A. K. Percy, "Rett syndrome: 1H spectroscopic imaging at 4.1 Tesla," Journal of ... A. L. Reiss, F. Faruque, S. Naidu et al., "Neuroanatomy of Rett syndrome: a volumetric imaging study," Annals of Neurology, vol ...
We developed a pipeline for whole brain image analysis that includes supervised machine learning (pixel-wise random forest ... We developed a pipeline for whole brain image analysis that includes supervised machine learning (pixel-wise random forest ... STPT automatically generates high-resolution images of coronal sections of the entire mouse brain that can be readily ... STPT automatically generates high-resolution images of coronal sections of the entire mouse brain that can be readily ...
... imaging of intrinsic optical signals, and voltage-sensitive dye-based imaging (Orbach et al., 1985; Grinvald et al., 1988; ... Imaging cortical dynamics at high spatial and temporal resolution with novel blue voltage-sensitive dyes. Neuron 24: 791-802. ... 1B). To clarify the identity of the imaged cells, mixtures of a Ca2+-sensitive dye and different cell-specific markers (e.g., ... which provides a targeted staining of neuronal populations with calcium-sensitive dyes (Stosiek et al., 2003). The cells are ...
It is implemented using optogenetic technology in tandem with imaging of voltage-sensitive dyes (VSD) or genetically encoded ... Voltage-sensitive fluorescence signals result from the Stark effect - modulation of the dyes electronic configuration by the ... All-optical electrophysiology obviates such challenges with the use of optically actuated depolarisation and voltage sensitive ... generating voltage-sensitive fluorescence responses of 24 per cent per 100mV. Following ChR2 stimulation (475nm, 0.8mW/mm2, ...
Ferezou I, Bolea S, Petersen CC (2006) Visualizing the cortical representation of whisker touch: voltage-sensitive dye imaging ... Mayer ML, Westbrook GL, Guthrie PB (1984) Voltage-dependent block by Mg2+ of NMDA responses in spinal cord neurones. Nature 309 ... Stuart G, Spruston N (1998) Determinants of voltage attenuation in neocortical pyramidal neuron dendrites. J Neurosci 18:3501- ... Cook EP, Johnston D (1999) Voltage-dependent properties of dendrites that eliminate location-dependent variability of synaptic ...
2009) In vivo voltage-sensitive dye imaging in adult mice reveals that somatosensory maps lost to stroke are replaced over ... In addition, they mapped how the forepaw was represented in the mices cortex using optical intrinsic signal imaging, a method ... harnessed optical intrinsic signal imaging to examine the state of these networks in mice. They found that following ... Here, we combine optogenetic photostimulation with optical intrinsic signal imaging to examine how contralesional excitatory ...
Palette of fluorinated voltage-sensitive hemicyanine dyes. Year: 2012. Authors: Yan P., Acker C.D., Zhou W.L., Lee P., ... and imaging of spatiotemporal patterns of action potential propagation in excitable tissues, such as the brain or heart. ... However, the available voltage-sensitive dyes (VSDs) are not always spectrally compatible with newly available optical ... We show the sensitivity to voltage and the photostability of the new VSDs in a series of experimental preparations ranging in ...
... also provides a unique assay of network activity that compliments alternative techniques such as voltage-sensitive dyes and ... Imaging of glutamate in brain slices using FRET sensors JOURNAL OF NEUROSCIENCE METHODS Dulla, C., Tani, H., Okumoto, S., ... Using glutamate biosensor imaging, we found that glutamate signals propagated throughout large areas of FL cortex and were ... Glutamate biosensor imaging reveals dysregulation of glutamatergic pathways in a model of developmental cortical malformation. ...
Optical Imaging 25% * 2-(2-(2-chloro-3-((1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene)ethylidene)-1-cyclohexen-1-yl) ... Voltage-sensitive dye delivery through the blood brain barrier using adenosine receptor agonist regadenoson. ... Dive into the research topics of Voltage-sensitive dye delivery through the blood brain barrier using adenosine receptor ...
In Silico Voltage-Sensitive Dye Imaging Reveals the Emergent Dynamics of Cortical Populations. Taylor H. Newton, Michael W. ... ITIS has released four new mouse models segmented from magnetic resonance imaging data. All four models - a pregnant model, a ... A Calibrated Physical Flow Standard for Medical Perfusion Imaging. Gertjan Kok, Nikola Pelevic, Amedeo Chiribiri, Xenios ... MARTIN V1.0 was developed by the Athinoula A. Martinos Center for Biomedical Imaging at Massachusetts General Hospital, Boston ...
Scalable classification in large scale spatiotemporal domains applied to voltage-sensitive dye imaging. Vainer, I., Kraus, S., ... Biomedical super resolved imaging using special micro-probe. Zalevsky, Z., Shahmoon, A. & Slovin, H., 2010, 2010 3rd ... Learning Canonical Representations for Scene Graph to Image Generation. Herzig, R., Bar, A., Xu, H., Chechik, G., Darrell, T. ... Joint optimization for cooperative image captioning. Vered, G., Oren, G., Atzmon, Y. & Chechik, G., Oct 2019, Proceedings - ...
... and voltage-sensitive dyes (VSD) as well as functional magnetic resonance imaging (fMRI) (in collaboration with TP1). While ... Die durch diese ESF-GS geförderten OVGU-Profillinien sind die Zentren für Neurowissenschaften (CBBS) und für die Dynamischen ... Dies stärkt die regionale Wirtschaft bei der Integration von Assistenzsystemen für die Industrie 4.0 im demographischen Wandel ... auf die Spezies Maus und die Zurverfügungstellung der Gewebe für die protemomischen Untersuchungen im Rahmen des Teilprojektes ...