Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst | Science
The pluripotency of SCNT-hES-1 cells was investigated in vitro (fig. S1) and in vivo (Fig. 3). Clumps of the cells were ... SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we ... cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell ... five ES cell lines were derived from a total of 14 ICMs, two ES cell lines were derived from four ICMs, and three ES cell lines ...http://science.sciencemag.org/content/303/5664/1669.full
Methods for treating diabetes by delivering insulin from biocompatible cell-containing devices - Patent # 5869077 -...
... living cells which secrete insulin, said cells being dispersed in a biocompatible matrix comprising a hydrogel or extracellular ... thermoplastic or hydrogel free of said cells projecting externally thereof, said jacket being permselective and immunoisolatory ... or from cells which replicate in vitro (i.e., immortalized cells or cell lines, including genetically modified cells). In all ... BHK-NGF Cell Line Production. Two human genomic clones (phbeta N8D8, phbeta N8B9) coding for the 5' and 3' ends of the .beta.- ...http://www.patentgenius.com/patent/5869077.html
Stem Cell Basics by Mai2xs - issuu
Title: Stem Cell Basics, Author: Mai2xs, Name: Stem Cell Basics, Length: 8 pages, Page: 1, Published: 2008-04-02 ... Growth of cells in vitro on an artificial medium for experimental research. Clone - A line of cells that is genetically ... Also, a single adult stem cell should be able to generate a line of genetically identical cells - known as a clone - which then ... and appear genetically normal, are referred to as an embryonic stem cell line. Once cell lines are established, or even before ...https://issuu.com/mai2xs/docs/stem_cell_basics
Peculiarities of somatic embryogenesis of long-term proliferating embryogenic cell lines of Larix sibirica in vitro, Russian...
"Peculiarities of somatic embryogenesis of long-term proliferating embryogenic cell lines of Larix sibirica in vitro, Russian ... and cell line 6 and cloned seedlings of this line were genetically stable and corresponded to the donor tree. Embryogenic cell ... An epigenetic view of plant cells cultured in vitro: somaclonal variation and beyond ... and cell line 6 and cloned seedlings of this line were genetically stable and corresponded to the donor tree. Embryogenic cell ...https://www.deepdyve.com/lp/springer_journal/peculiarities-of-somatic-embryogenesis-of-long-term-proliferating-AkvvfmbMQT
No Case for Embryonic Stem Cells Research
Technical and financial hurdles add to ethical and safety concerns over embryonic stems cells while adult stem cells are ... Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst. Sciencexpress report 12 February ... Therapeutic cloning gives genetically and epigenetically defective ES cells. Reproductive cloning is now almost universally ... Moreover, their capacity to differentiate spontaneously into different cell types under in vitro conditions is variable . ...http://www.i-sis.org.uk/NCFESC.php
Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts | PNAS
2001) Pluripotency of bovine embryonic cell line derived from precompacting embryos. Cloning 3:59-67. ... Another potential use of these cells is the in vitro differentiation to gametes, facilitating in vitro breeding schemes that ... single-cell dissociation using trypsin). These are all desirable features that will facilitate the creation of genetically ... 2007) New cell lines from mouse epiblast share defining features with human embryonic stem cells. Nature 448:196-199. ...https://www.pnas.org/content/115/9/2090
Abstract #812: Efficacy of Antiprogestin in genetically engineered T47D cells overexpressing aromatase | Cancer Research
The aromatase expression of single-cell clone from T47D cell line (T47Darom) was confirmed by RT-PCR, Western blot, Estrone ... Determination of the efficacy of aromatase inhibitors in vitro has been difficult since the known breast cancer cells express ... Previous studies have utilized genetically engineered aromatase expressing cell lines, however these cells were derived from ... was cloned in mammalian expression vector and was stably transfected into T47D breast cancer cell lines keeping empty vector as ...http://cancerres.aacrjournals.org/content/69/9_Supplement/812
Lactate Influx through the Endothelial Cell Monocarboxylate Transporter MCT1 Supports an NF-κB/IL-8 Pathway that Drives Tumor...
... we used tumor cells genetically modified for their capacity to handle lactate. Accordingly, we established clones from the WiDr ... of cell proliferation rate in vitro however did not reveal any difference between shMCT1 and shMCT4 WiDr cell clones (Fig. 5C ... adenocarcinoma WiDr cells and MDA-MB231 breast cancer cells were obtained from the American Type Culture Collection Cell lines ... 105 WiDr shMCT4 cells mixed with 5 × 104 HUVEC cells; (ii) 5 × 105 MDA-MB231 or 5 × 105 MDA-MB231 shMCT4 cells mixed with 5 × ...http://cancerres.aacrjournals.org/content/71/7/2550
T cell epitopes of the major allergens from dermatophagoides (house dust mite) - Patent # 7288256 - PatentGenius
Peptides within the scope of the invention comprises at least one T cell epitope, or preferably at least two T cell epitopes of ... MHC responsive cells can then be cloned by limiting dilution and permanent lines expanded and maintained by periodic in vitro ... Monoclonal T cell populations (i.e., T cells genetically identical to one another andexpressing identical T cell receptors) can ... These EBV-transformed cell lines were made by incubating5.times.10.sup.6 PBL with 1 ml of B-59/8 Marmoset cell line (ATCC ...http://www.patentgenius.com/patent/7288256.html
Immortalised cell line - Wikipedia
Some of them are normal cell lines (e.g. derived from stem cells). Other immortalised cell lines are the in vitro equivalent of ... Immortalized cell lines can also be cloned giving rise to a clonal population which can, in turn, be propagated indefinitely. ... Further, cell lines can change genetically over multiple passages, leading to phenotypic differences among isolates and ... Immortalised cell lines have also found uses in biotechnology. An immortalized cell line should not be confused with stem cells ...https://en.wikipedia.org/wiki/Immortalised_cell_line
Ethics of Stem Cell Research (Stanford Encyclopedia of Philosophy)
Hayashi, K. et al., 2012, "Offspring from Oocytes Derived from In Vitro Primordial Germ Cell-Like Cells in Mice," Science, 338( ... problem is through the creation of a public stem cell bank that represents a genetically diverse pool of stem cell lines (Faden ... From a research standpoint, creating embryos through cloning technologies with cells that are known to have particular genetic ... Yu, J., et al., 2007, "Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells," Science 318: 1917-1920. ...https://plato.stanford.edu/entries/stem-cells/
Patent US6297219 - Site-specific instillation of cells or site-specific transformation of cells ... - Google Patents
A method for the direct in vivo transformation of cells in and surrounding a solid tumor is disclosed. This method is based on ... followed by the introduction of genetically altered cells into the host. An illustrative packaging cell line which may be used ... by relying upon the use of vascular smooth muscle cells or endothelia cells which have been transduced in vitro as a vehicle to ... Second, the gene to be expressed must be cloned and available for genetic manipulation. Third, the gene must be introduced into ...http://www.google.com/patents/US6297219?dq=2040248
Induced stem cells - Wikipedia
2015). "A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs". Nature Biotechnology. ... "Residual Undifferentiated Cells During Differentiation of Induced Pluripotent Stem Cells In Vitro and In Vivo". Stem Cells and ... Induced androgenetic haploid embryonic stem cells can be used instead of sperm for cloning. These cells, synchronized in M ... human iPS cell-derived myeloid cell lines as unlimited cell source for dendritic cell-like antigen-presenting cells". Gene ...https://en.wikipedia.org/wiki/Induced_stem_cells
UK scientists to clone human embryos | New Scientist
The first licence to be granted in Europe allows the harvesting of stem cells - researchers hope it will lead to therapies for ... The advantage of cloning an embryo from a specific patient would be that the stem cells produced would be genetically identical ... "Even with ESCs from fertilised embryos, it takes a year to get a fully characterised stem cell line," he told New Scientist. ... The team will use unfertilised eggs donated by women undergoing in-vitro fertilisation (IVF) procedures. Stojkovic estimates ...https://www.newscientist.com/article/dn6272-uk-scientists-to-clone-human-embryos/
Cancer immunotherapy with recombinant poliovirus induces IFN-dominant activation of dendritic cells and tumor antigen-specific...
In vitro CTL assay. Tumor cell lines and RNA-electroporated DCs were used as targets. Cells were harvested, washed to remove ... a genetically engineered murine astrocytoma line [derived from a mutant Nf1 and Trp53 mouse model (60); a gift from J. Sampson ... Cloning of tumor antigen complementary DNA (cDNA) and production of tumor antigen-encoding RNA were performed using standard ... For in vitro studies, experimental findings were confirmed using multiple cell lines and cells from healthy volunteers (n = 3 ...https://stm.sciencemag.org/content/9/408/eaan4220
Androgen-Regulated and Highly Tumorigenic Human Prostate Cancer Cell Line Established from a Transplantable Primary CWR22 Tumor...
Future studies need to characterize genetically and epigenetically the cell clones that recur when CWR22Pc cells are grown as ... A new human prostate carcinoma cell line, 22Rv1. In Vitro Cell Dev Biol Anim 1999;35:403-09. ... We show that the CWR22Pc cell line genetically originates from the primary CWR22 tumors. The growth of CWR22Pc cells is ... D, flow cytometric analysis of CWR22Pc cells shows that the cell line consists of a mixed population of cells with DNA indices ...http://clincancerres.aacrjournals.org/content/14/19/6062.long
Phorbol 12-Myristate 13-Acetate Protects Jurkat Cells from Methylglyoxal-Induced Apoptosis by Preventing c-Jun N-Terminal...
Clones derived from each cell line were selected with 2 mg/ml G418. Jurkat cells were suspended at 2.5 × 105/ml in RPMI 1640 ... Cells were maintained at 37°C in a humidified atmosphere containing 5% CO2. Jurkat cells genetically deficient in caspase-8 ... Cells were resuspended at 1 × 106/ml in RPMI 1640 medium supplemented with 5% FCS for in vitro culture study. ... We next assessed the levels of apoptosis by stimulating three cell lines, Jurkat cells, Ras WT cells, and Ras N17 cells, with ...http://molpharm.aspetjournals.org/content/65/3/778
US5830995A - Fanphiregulins: a family of heparin-binding epithelial cell growth factors - Google Patents
... accelerating the growth of normal epithelial cells. In some tumor cell lines, primarily of breast or ovarian carcinomas, AR ... AR is detectable in several normal human epithelial cells and in a high percentage of human colon cancers. In addition to ... stimulating the growth of these cultured epithelial cells, AR may have a wide range of applications including, but not limited ... for disease staging and prognosis and reagents that specifically block AR may be of therapeutic use in controlling tumor cell ...https://patents.google.com/patent/US5830995A/en
A Monoclonal Antibody Reactive with a 40-kDa Molecule on Fetal Thymocytes and Tumor Cells Blocks Proliferation and Stimulates...
E710.2.3 is a cloned murine CD4−CD8− thymic T lymphoma cell line. It was isolated from a thymic tumor from an AKR/J mouse that ... or since it is expressed on cultured cell lines may be the result of long term in vitro culture. ... AKR mice are genetically predisposed to developing tumors of the thymus and develop thymic lymphomas at high frequency after 5 ... Positive cell lines included some T cell lines (e.g., RMA-S), several B cell lymphomas (e.g., A20 and WEHI-231), and a ...http://www.jimmunol.org/content/163/3/1306
Volume 131, Issue 18 | Blood Journal
Stable lines and clones of long-term proliferating normal, genetically unmodified murine common lymphoid progenitors. Kawano ... These cells retain the ability to differentiate into all lymphoid lineages in vitro and in vivo. ... High-grade B-cell lymphoma with MYC and BCL2 and/or BCL6 rearrangements with diffuse large B-cell lymphoma morphology. Scott ... BTKCys481Ser drives ibrutinib resistance via ERK1/2, and protects BTKwild-type MYD88-mutated cells by a paracrine mechanism. ...http://www.bloodjournal.org/page/this-week-in-blood/may-3-2018?sso-checked=true
Frontiers | Astrocyte Apoptosis and HIV Replication Are Modulated in Host Cells Coinfected with Trypanosoma cruzi | Cellular...
In this report, human astrocytes were infected in vitro with both genetically modified-pathogens to express alternative ... In this context, lower rates of cell death were observed in both T. cruzi monoinfected-astrocytes and HIV-T.cruzi coinfection ... In this context, lower rates of cell death were observed in both T. cruzi monoinfected-astrocytes and HIV-T.cruzi coinfection ... rate or its increased intracellular presence but does not require their intracellular cohabitation or infected cell-to-cell ...https://www.frontiersin.org/articles/10.3389/fcimb.2017.00345/full
The best book series to read, ed50 effektive dosis, first aid treatment record form, what is edema reduction
Most cells grown in vitro for laboratory use are 'cell lines': cells of the type we're interested in that have a nearly ... These are usually cancer cells, or cells that have been genetically modified to permit ongoing proliferation. However, neither ... on the space journey to Venus caused a mutation in the CYP2B6 allele in the cell that Flo happened to use in her cloning; the ... Radiation-induced apoptosis in human non-small-cell lung cancer cell lines is secondary to cell-cycle progression beyond the G2 ...http://s3.amazonaws.com/awakebed4survive/ed50-effektive-dosis.html
Decreased expression of the β2 integrin on tumor cells is associated with a reduction in liver metastasis of colorectal cancer...
Cancer cell lines. All in vitro and in vivo experiments were conducted using the murine C26 colon adenocarcinoma (C26) cell ... GAPDH was used as an internal control (house keeping gene) (b) Protein lysates were obtained from 1 clone cell line clones as ... The C26 cell line was genetically modified to partially deplete the expression of the β2 subunit of the LFA-1 receptor (named β ... The black line represents C26 cells, the red line represents β2-C26 cells and dash line represents the negative control. d ...https://bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3823-2
Cells Expressing Indoleamine 2,3-Dioxygenase Inhibit T Cell Responses | The Journal of Immunology
C, Flow cytometric analyses of transfected MC57G cells stained with H-2Kb-specific mAb MC57G clones (dotted lines). Bold lines ... These outcomes suggest that genetically enhanced IDO activity inhibited T cell proliferation in vitro and in vivo. Genetic ... IDO-transfected cells inhibit in vitro T cell proliferation. IDO-transfected MC57G cells were cocultured with splenocytes from ... To directly evaluate the hypothesis that cells expressing IDO inhibit T cell responses, we prepared IDO-transfected cell lines ...http://www.jimmunol.org/content/168/8/3771.long
Script for video - "Stem Cells"
Unlike a lot of cell lines that we grow routinely in the lab, we know how to grow very well, these cells are very finicky and ... Now what cloning does is provide the opportunity to multiply these cells, engineer the cells in transplantation ... These ... One is in-vitro fertilization lab to simply make an embryo specifically to retrieve its stem cells. The second is to use ... of your own cells in order to derive stem cells from it which would in turn be developed into tissue that now is genetically ...http://hawkhill.com/scripts/929s.html