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*  Quantitative enzyme histochemistry of steroid and glucuronide synthesis in testes and seminal vesicle, and its correlation to...
In spawning Clarias gariepinus from the Hula Nature Reserve in Northern Israel, the mean value for the seminal vesicle somatic index (SVSI) had decreased as compared with that in prespawning fish, due to loss of seminal vesicle fluid. Mean gonadosomatic index (GSI), however, had increased, probably as a result of ... read more hydration, 3α-Hydroxysteroid dehydrogenase (3αHSD), 3βHSD and uridine diphosphoglucose dehydrogenase (UDPGD) reactions were demonstrated in interstitial cells of both testis and seminal vesicle. UDPGD reactions were also found in the epithelium of the seminal vesicle tubules. Quantitative determination of the enzyme reactions with a computerized image analysis system revealed that total activity of all three enzymes in interstitial cells of the seminal vesicle was distinctly stronger in spawning fish. Prespawning and spawning fish did not differ in total UDPGD activity in the epithelium lining the seminal vesicle ...
https://dspace.library.uu.nl/handle/1874/17491
*  Regulation of UDP-glucose dehydrogenase is sufficient to modulate hyaluronan production and release, control sulfated GAG...
Glycosaminoglycans (GAGs) are critical for extracellular matrix (ECM) integrity in cartilage but mechanisms regulating their synthesis are not defined. UDP-glucose dehydrogenase (UGDH) catalyses UDP-glucose oxidation to UDP-glucuronic acid, an essential monosaccharide in many GAGs. Our previous studies in articular surface (AS) cells from embryonic joints have established pivotal roles for mitogen-activated protein kinases (MAPK) in synthesis of the unsulfated GAG, hyaluronan (HA). We investigated the functional significance of UGDH in GAG production and chondrogenesis, and determined roles for MEK-ERK and p38MAPK pathways in regulating UGDH expression and function. Inhibitors of MEK and p38MAPK reduced UGDH protein in AS cells. Treatment with TGF-? (archetypal growth factor) increased UGDH expression, sulfated (s)-GAG/HA release and pericellular matrix formation in a p38MAPK-dependent manner. Retroviral overexpression of UGDH augmented HA/sGAG release and ...
https://eprints.soton.ac.uk/339885/
*  Uridine Diphosphate Glucose Dehydrogenase Summary Report | CureHunter
Uridine Diphosphate Glucose Dehydrogenase: An enzyme that catalyzes the oxidation of UDPglucose to UDPglucuronate in the presence of NAD+. EC 1.1.1.22.
http://www.curehunter.com/public/keywordSummaryD014533-Uridine-Diphosphate-Glucose-Dehydrogenase.do
*  Uridine diphosphate glucuronic acid - Wikipedia
UDP glucuronic acid is a sugar used in the creation of polysaccharides and is an intermediate in the biosynthesis of ascorbic acid (except in primates and guinea pigs). It is made from UDP-glucose by UDP-glucose 6-dehydrogenase (EC 1.1.1.22) using NAD+ as a cofactor. It is the source of the glucuronosyl group in glucuronosyltransferase reactions. Glucuronic acid UDP Bontemps Y, Vuillermoz B, Antonicelli F, Perreau C, Danan JL, Maquart FX, Wegrowski Y (Jun 2003). "Specific protein-1 is a universal regulator of UDP-glucose dehydrogenase expression: its positive involvement in transforming growth factor-beta signaling and inhibition in hypoxia". The Journal of Biological Chemistry. 278 (24): 21566-75. doi:10.1074/jbc.M209366200. PMID 12682078. Sommer BJ, Barycki JJ, Simpson MA (May 2004). "Characterization of human UDP-glucose dehydrogenase. CYS-276 is required for the second of two successive ...
https://en.wikipedia.org/wiki/Uridine_diphosphate_glucuronic_acid
*  cDNA cloning and expression of UDP-glucose dehydrogenase from bovine kidney
Polysaccharides are found in all forms of life and serve diverse purposes. They are enzymatically synthesised from activated monosaccharide precursors, nucleotide sugars. One such nucleotide sugar is UDP-glucuronic acid, which is formed from UDP-glucose by the UDP-glucose dehydrogenase (UGDH) enzyme. UGDH has been proposed to have a regulatory role in the biosynthesis of polysaccharides. The aim of the studies presented in this thesis was to investigate the role of UGDH in the polysaccharide biosynthesis in three different systems: human cell culture, bacterial cultures and growing plants. The effects of UGDH-overexpression on polysaccharide biosyntheses and, when achievable, on UDP-sugar levels, were investigated.. A mammalian UGDH was cloned from a kidney cDNA library. Transient expression of the cloned enzyme in mammalian cells led to an increased UGDH-activity. Northern blotting analyses revealed a single transcript of 2.6 kb in adult mouse tissues ...
http://uu.diva-portal.org/smash/record.jsf?pid=diva2:165403
*  UDP-glucose dehydrogenase: structure and function of a potential drug target.
Biosynthesis of the glycosaminoglycan precursor UDP-α-D-glucuronic acid occurs through a 2-fold oxidation of UDP-α-D-glucose that is catalysed by UGDH (UDP-α-D-glucose 6-dehydrogenase). Structure-function relationships for UGDH and proposals for the enzymatic reaction mechanism are reviewed in the present paper, and structure-based sequence comparison is used for subclassification of UGDH family members. The eukaryotic group of enzymes (UGDH-II) utilize an extended C-terminal domain for the formation of complex homohexameric assemblies. The comparably simpler oligomerization behaviour of the prokaryotic group of enzymes (UGDH-I), in which dimeric forms prevail, is traced back to the lack of relevant intersubunit contacts and trimmings within the C-terminal region. The active site of UGDH contains a highly conserved cysteine residue, which plays a key role in covalent catalysis. Elevated glycosaminoglycan formation is implicated in a variety of human ...
https://oacentre.kennedy.ox.ac.uk/publications/108659
*  中国科学院水生生物研究所机构知识库(IHB OpenIR): Cloning and characterization of the gene for UDPGlc dehydrogenase from the cyanobacterium,...
Using degenerate primers based on conserved regions of the UDP-glucose dehydrogenase (UDPGDH) gene, an initial 476-bp DNA fragment was amplified from the water-bloom forming cyanobacterium, Microcystis aeruginosa FACHB 905. TAIL-PCR and ligation-mediated PCR were used to amplify the flanking regions to isolate an about 2.5-kb genomic DNA fragment. Sequence analysis revealed an ORF encoding a putative 462 amino acid protein, designated Mud for Microcystis UDPGDH. The Mud amino acid sequence is closely related to UDPGDH sequences from cyanobacterium Synechocystis PCC6803 (73% identity, 81% similarity), and bacterium Bacillus subtilis (51% identity and 67% similarity). The cloned mud gene was expressed in Escherichia coli using the pGEX-4T-1 fusion expression vector system to generate a GST-Mud fusion protein that exhibited UDPGDH activity. The cytosolic fraction of M aeruginosa FACHB 905 was subjected to Western analysis with an anti-Mud antibody, which revealed a single band ...
http://ir.ihb.ac.cn/handle/152342/9390
*  Interactive effects of phosphate, sugar and light/ dark conditions on gene expression of UDP-glucose pyrophosphorylase in...
Photosynthesis captures light from the sun and converts it into carbohydrates, which are utilised by almost all living organisms. The conversion between the different forms of carbohydrates is the basis to form almost all biological molecules.. The main intention of this thesis has been to study the role of UDP-glucose in carbohydrate synthesis and metabolism, and in particular the genes that encode UDP-glucose pyrophosphorylase (UGPase) and UDP-glucose dehydrogenase (UGDH) in plants and their regulation. UGPase converts glucose-1-phosphate to UDP-glucose, which can be utilised for sucrose synthesis, or cell wall polysaccharides among others. UGDH converts UDP-glucose to UDP-glucuronate, which is a precursor for hemicellulose and pectin. As model species I have been working with both Arabidopsis thaliana and poplar.. Sequences for two full-length EST clones of Ugp were obtained from both ...
http://umu.diva-portal.org/smash/record.jsf?pid=diva2:145084
*  Detection of genetic association and functional polymorphisms of UGDH affecting milk production trait in Chinese Holstein...
In this study, we provide a strong evidence for the significant associations of UGDH, with 2 milk production traits in a Chinese Holstein cattle population. Moreover, we demonstrated that the SNPs Ex1-1 and Ex11-1 in exonic regions of UGDH are two functional polymorphisms in which A allele of SNP Ex1-1 and T allele of SNP Ex11-1 cause lower expression level of UGDH compared with the G and C allele respectively. Together, our findings not only confirmed our early finding that a QTL located in the 1.5-Mb region between BMS483 and MNB-209 for milk yield and protein yield[14], but also strongly suggest that the SNPs Ex1-1 and Ex11-1 in UGDH might be QTN responsible for this QTL.. Previously, we mapped a QTL near BMS470 with effects on milk production traits with daughter design using 14 microsatellites on bovine chromosome 6 in a Chinese Holstein population involving 26 paternal half-sib families with 2356 daughters[8, 9]. By increasing the marker density with 17 microsatellite markers spanning from ...
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-13-590
*  Glycosaminoglycan-dependent restriction of FGF diffusion is necessary for lacrimal gland development | Development
Glycosaminoglycans (GAGs) play a central role in embryonic development by regulating the movement and signaling of morphogens. We have previously demonstrated that GAGs are the co-receptors for Fgf10 signaling in the lacrimal gland epithelium, but their function in the Fgf10-producing periocular mesenchyme is still poorly understood. In this study, we have generated a mesenchymal ablation of UDP-glucose dehydrogenase (Ugdh), an essential biosynthetic enzyme for GAGs. Although Fgf10 RNA is expressed normally in the periocular mesenchyme, Ugdh mutation leads to excessive dispersion of Fgf10 protein, which fails to elicit an FGF signaling response or budding morphogenesis in the presumptive lacrimal gland epithelium. This is supported by genetic rescue experiments in which the Ugdh lacrimal gland defect is ameliorated by constitutive Ras activation in the epithelium but not in the mesenchyme. We further show that lacrimal gland development requires the mesenchymal expression of ...
http://dev.biologists.org/content/early/2012/06/28/dev.079236
*  Most recent papers with the keyword lead toxicity | Read by QxMD
6-Thiopurine (6TP) is an actively prescribed drug in the treatment of various diseases ranging from Crohn's disease and other inflammatory diseases to acute lymphocytic leukemia and non-Hodgkin's leukemia. While 6TP has beneficial therapeutic uses, severe toxicities are also reported with its use, such as jaundice and liver toxicity. While numerous investigations into the mode in which toxicity originates has been undertaken. None have investigated the effects of inhibition towards UDP-Glucose Dehydrogenase (UDPGDH), an oxidative enzyme responsible for UDP-glucuronic acid (UDPGA) formation or UDP-Glucuronosyl transferase (UGT1A1), which is responsible for the conjugation of bilirubin with UDPGA for excretion ...
https://www.readbyqxmd.com/keyword/97490
*  Glycosaminoglycan-dependent restriction of FGF diffusion is necessary for lacrimal gland development | Development
Glycosaminoglycans (GAGs) are the major constituents of the extracellular matrix that control the transport and signaling of numerous growth factors (Hacker et al., 2005). Consisting of 50-400 repeats of disaccharide units, GAGs can be divided by their composition, sulfation and epimerization into chondroitin sulfate (CS), dermatan sulfate (DS), heparan sulfate (HS)/heparin, and keratan sulfate (KS). A common precursor of all GAGs is UDP-glucuronic acid, which is synthesized from UDP-glucose by a single mammalian enzyme, UDP-glucose dehydrogenase (Ugdh). Together with an amino sugar such as N-acetylglucosamine for HS and N-acetylgalactosamine for CS, these monosaccharides are incorporated by polymerization enzymes into the backbone of the polysaccharide and are further modified by a series of sulfotransferase enzymes (Esko and Selleck, 2002). For example, the polymerization of HS is exclusively catalyzed by Ext enzymes, which are followed by ...
http://dev.biologists.org/content/139/15/2730
*  The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall...
The nucleotide sugar UDP-glucuronic acid (UDP-GlcA) is the principal precursor for galacturonic acid, xylose, apiose and arabinose residues of the plant cell-wall polymers. UDP-GlcA can be synthesized by two different functional pathways in Arabidopsis involving either UDP-glucose dehydrogenase or inositol oxygenase as the initial enzyme reaction to channel carbohydrates into a pool of UDP sugars used for cell-wall biosynthesis. The genes for the enzyme myo-inositol oxygenase (MIOX) were analyzed in Arabidopsis. They represent a small gene family containing four members. The transcription of all those members indicates a transient and organ-specific gene expression pattern in growing plant tissues as analyzed by RT-PCR and in promoter::GUS reporter gene lines. Two isoforms (MIOX1, MIOX2) are expressed in almost all tissues of the plant, whereas the expression of MIOX4 and MIOX5 is largely restricted to flowers, particularly maturing pollen. T-DNA insertion lines in MIOX ...
https://pub.uni-bielefeld.de/publication/1996216
*  UDP-glucose 6-dehydrogenase - Wikipedia
UDP-glucose 6-dehydrogenase is a cytosolic enzyme that in humans is encoded by the UGDH gene. The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. This enzyme participates in 4 metabolic pathways: pentose and glucuronate interconversions, ascorbate and aldarate metabolism, starch and sucrose metabolism, and nucleotide sugars metabolism. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is ...
https://en.wikipedia.org/wiki/UDP-glucose_6-dehydrogenase
*  RCSB PDB - Protein Feature View - UDP-glucose dehydrogenase - A4UTT2 (A4UTT2 SPHEL)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
http://www.rcsb.org/pdb/protein/A4UTT2
*  UDP-glucose 4-epimerase - Wikipedia
The enzyme UDP-glucose 4-epimerase (EC 5.1.3.2), also known as UDP-galactose 4-epimerase or GALE, is a homodimeric epimerase found in bacterial, fungal, plant, and mammalian cells. This enzyme performs the final step in the Leloir pathway of galactose metabolism, catalyzing the reversible conversion of UDP-galactose to UDP-glucose. GALE tightly binds nicotinamide adenine dinucleotide (NAD+), a co-factor required for catalytic activity. Additionally, human and some bacterial GALE isoforms reversibly catalyze the formation of UDP-N-acetylgalactosamine (UDP-GalNAc) from UDP-N-acetylglucosamine (UDP-GlcNAc) in the presence of NAD+, an initial step in glycoprotein or glycolipid synthesis. Dr. Luis Leloir deduced the role of GALE in galactose metabolism during his tenure at the Instituto de Investigaciones Bioquímicas del Fundación Campomar, initially terming the enzyme waldenase. Dr. Leloir was awarded the 1970 Nobel Prize in Chemistry for his discovery of sugar nucleotides and ...
https://en.wikipedia.org/wiki/UDP-glucose_4-epimerase
*  RCSB PDB - 1BGT: CRYSTAL STRUCTURE OF THE DNA MODIFYING ENZYME BETA-GLUCOSYLTRANSFERASE IN THE PRESENCE AND ABSENCE...
1BGT: Crystal structure of the DNA modifying enzyme beta-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose.
http://www.rcsb.org/pdb/explore/derivedData.do?structureId=1BGT
*  Redox regulation of mitochondrial sulfide oxidation in the lugworm, Arenicola marina | Journal of Experimental Biology
Reactive oxygen species (ROS) are produced mainly by mitochondria when electrons leak from a highly reduced respiratory chain (Andreyev et al., 2005). Apart from causing oxidative damage to several cellular constituents, ROS also function as signal molecules (Moran et al., 2001; Damdimopoulos et al., 2002).. Protein sulfhydryl groups are crucial to redox regulation as they can be reversibly oxidized, forming inter- or intra-molecular disulfide bridges (Ghezzi, 2005). The plant AOX is a dimer covalently linked by a disulfide bridge that has to be reduced and form a thiohemiacetal with pyruvate in order to produce the active conformation (Rhoads et al., 1998; Vanlerberghe et al., 1998). Thus, the redox state regulates the partitioning of electron flux between AOX and cytochrome oxidase in plant mitochondria (Berthold et al., 2000). The lugworm AOX may be regulated in a similar way, as the detoxifying sulfide oxidation pathway was active exclusively under reducing conditions. However, sulfide but ...
http://jeb.biologists.org/content/211/16/2617
*  ugdh Summary [species: Xenopus laevis and Xenopus tropicalis] - Xenbase Gene Catalog
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari. ...
http://www.xenbase.org/gene/showgene.do?method=display&geneId=950969
*  Uxs1 MGI Mouse Gene Detail - MGI:1915133 - UDP-glucuronate decarboxylase 1
View mouse Uxs1 Chr1:43746966-43827800 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
http://www.informatics.jax.org/marker/MGI:1915133
*  WikiGenes - UXS1 - UDP-glucuronate decarboxylase 1
The world's first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.
https://www.wikigenes.org/e/gene/e/80146.html
*  UDP-glucose 4-épimérase - Wikipedia
UDP-glucose 4-épimérase Dimère d'UDP-glucose 4-épimérase humaine (PDB 1EK5) L'UDP-glucose 4-épimérase, ou UDP-galactose 4-épimérase (GALE), est une épimérase homodimérique présente chez les bactéries, les mycètes, les plantes et les mammifères, qui catalyse la réaction : UDP-glucose ⇌ {\displaystyle \rightleftharpoons } UDP-galactose. Cette enzyme intervient à l'étape finale de la voie de Leloir, de dégradation du galactose. Elle requiert du NAD+ comme cofacteur. Chez l'homme et chez certaines bactéries, cette enzyme est également capable d'agir sur l'UDP-N-acétylglucosamine pour former de l'UDP-N-acétylgalactosamine en présence de NAD+, étape initiale de la biosynthèse de glycoprotéines et de glycolipides. ↑ (en) James B. Thoden, Travis M. Wohlers, Judith L. Fridovich-Keil et Hazel M. Holden, « Crystallographic Evidence for Tyr 157 Functioning as the Active Site Base in Human UDP−Galactose 4-Epimerase », Biochemistry, vol. 39, no 19,‎ 16 ...
https://fr.wikipedia.org/wiki/UDP-glucose_4-%C3%A9pim%C3%A9rase
*  UDP-glucose | Abcam
Buy UDP-glucose (CAS 28053-08-9), a water soluble highly potent P2Y14 agonist. Join researchers using high quality UDP-glucose from Abcam and achieve your…
http://www.abcam.com/udp-glucose-ab120384.html
*  RCSB PDB - Protein Feature View - UDP-glucose 6-dehydrogenase - Q19905 (UGDH CAEEL)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
http://www.rcsb.org/pdb/protein/Q19905
*  SCOP2 - Hypothetical Evolutionary Event 1: Segment-swapping event within the dimeric biological unit of the UDP-glucose/GDP...
helix-swapping occurs in the dimerisation (middle) domain so that both the overall biological unit and the active site architecture are preserved
http://scop2.mrc-lmb.cam.ac.uk/event-1.html