Helicobacter species can colonise the mammalian gastrointestinal and hepatobiliary tract which usually results in a chronic infection coupled to an inflammatory host response. It is therefore not surprising that colonisation with Helicobacter species is linked with a range of inflammation associated gastrointestinal and hepatobiliary diseases.1 Recently, this range has been expanded, with an association of infection with enterohepatic Helicobacter species and the formation of cholesterol gallstones.2. In their study, Maurer and colleagues2 demonstrated that murine infection with the enterohepatic Helicobacter species H bilis and H hepaticus accelerated the formation of cholesterol gallstones in mice fed a lithogenic diet. Although the gallbladder mucosa in mice with gallstones displayed signs of inflammation, Helicobacter species were not cultured from the inflamed gallbladder or bile. Therefore, Maurer et al hypothesised that the chronic immune stimulation caused by Helicobacter species, rather ...
Hence we tested ureases effect upon O. fasciatus isolated midguts contractions. We observed that urease is capable of ... and to test the effects of urease and derived peptides upon isolated organs. When fed with different Jackbean major urease (JBU ... Observamos que a urease tem a capacidade de inibir, em algumas concentrações, o efeito excitatório da serotonina sobre as ... Concluímos com os dados gerados nesse estudo, e em estudos prévios, que a toxicidade das ureases não só é devida à ativação ...
When compared, the α subunits of Helicobacter pylori urease and other bacterial ureases align with the jack bean ureases, ... The structure of urease was first solved by P. A. Karplus in 1995. Urease was the first ever enzyme crystallized. Active site ... Urease activity tends to increase the pH of its environment as it produces ammonia, a basic molecule. Ureases are found in ... The presence of urease is used in the diagnosis of Helicobacter species. All bacterial ureases are solely cytoplasmic, except ...
The urease produced by H. pylori hydrolyzes urea to ammonia, which raises the pH of the medium, and changes the color of the ... Rapid urease test, also known as the CLO test (Campylobacter-like organism test), is a rapid diagnostic test for diagnosis of ... The basis of the test is the ability of H. pylori to secrete the urease enzyme, which catalyzes the conversion of urea to ...
1. urease, enzyme. usage: an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia; is present in ...
Urease (IPR008221). Short name: Urease Overlapping homologous superfamilies *Metal-dependent hydrolase, composite domain ... Urease (urea amidohydrolase, EC:3.5.1.5) catalyses the hydrolysis of urea to form ammonia and carbamate. The subunit ... Molecular biology of microbial ureases.. Microbiol. Rev. 59 451-80 1995. Jabri E, Carr MB, Hausinger RP, Karplus PA. The ... An evolutionary treasure: unification of a broad set of amidohydrolases related to urease.. Proteins 28 72-82 1997 ...
Urease_alpha (cd00375). Accession cd00375 Integration. Urease, alpha subunit (IPR005848) Member database. CDD. CDD is a protein ...
Soil urease activity was found to be more sensitive to soil temperature and pH rather than soil salinity. This ensured that ... Soil urease activity showed a decreasing pattern with increase in depth from the deep forest region of the Sundarban forest ... Soil ammonia concentration was found to be directly governed by the soil urease activity [The regression equation is Ammonia in ... soil urease along with the microbes present in the Sundarban forest ecosystem are more tolerant to fluctuation in salinity than ...
Potent covalent inhibitors of bacterial urease identified by activity-reactivity profiling.. Macegoniuk K1, Kowalczyk R2, ... Although the discovery of inhibitors of urease, a urea hydrolyzing enzyme crucial for the survival of some human pathogens, is ... Forty Michael acceptor-type compounds were screened for their inhibitory activities against bacterial urease, and several ... a field of medicinal chemistry that has grown in recent years, covalent urease inhibitors have been rarely investigated until ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
IPR011612, Urease_alpha_N_dom. IPR017950, Urease_AS. IPR005848, Urease_asu. IPR017951, Urease_asu_c. IPR029754, Urease_Ni- ... IPR011612, Urease_alpha_N_dom. IPR017950, Urease_AS. IPR005848, Urease_asu. IPR017951, Urease_asu_c. IPR029754, Urease_Ni- ... Urease subunit gamma (ureA), Urease subunit gamma (ureA), Urease subunit beta (ureB), Urease subunit alpha (ureC), Urea ... TIGR01792, urease_alph, 1 hit. PROSITEi. View protein in PROSITE. PS01120, UREASE_1, 1 hit. PS00145, UREASE_2, 1 hit. ...
Urease Broth, Prepared Media Tubes, Pack 10. Item # 827482 *bvseo_sdk, java_sdk, bvseo-4.0.0 ...
To study inhibitory effects of newly formed complexes, enzyme inhibition studies have been conducted against urease, α- ... To study inhibitory effects of newly formed complexes, enzyme inhibition studies have been conducted against urease, α- ... Enzymatic activity results of these complexes indicated them to be good inhibitors of urease enzyme while all complexes show ... Enzymatic activity results of these complexes indicated them to be good inhibitors of urease enzyme while all complexes show ...
MF_00739 Urease_gamma, 1 hit. InterProi. View protein in InterPro. IPR012010 Urease_gamma. IPR002026 Urease_gamma/gamma-beta_ ... Urease subunit gamma (ureA), Urease subunit alpha (ureC), Urease subunit beta (ureB) ... IPR012010 Urease_gamma. IPR002026 Urease_gamma/gamma-beta_su. IPR036463 Urease_gamma_sf. ... This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule ...
UreB is one of the urease subunits of Helicobacter pylori and can be used as an excellent antigen candidate for H. pylori ... UreB is one of the urease subunits of Helicobacter pylori and can be used as an excellent antigen candidate for H. pylori ... Affinity purification of Helicobacter pylori urease. Relevance to gastric mucin adherence by urease protein. J Biol Chem 273: ... Periplasmic expression and one-step purification of urease subunit B of Helicobacter pylori *Amirhooshang Alvandi. 1. , ...
A new proposal for urease mechanism based on the crystal structures of the native and inhibited enzyme from Bacillus pasteurii ... Description: PROTEIN (UREASE GAMMA SUBUNIT) protein , Length: 101 No structure alignment results are available for 2UBP.A ... Description: PROTEIN (UREASE BETA SUBUNIT) protein , Length: 122 No structure alignment results are available for 2UBP.B ... Description: PROTEIN (UREASE ALPHA SUBUNIT) protein , Length: 570 No structure alignment results are available for 2UBP.C ...
H. pyloris urease is an important virulence factor produced in large amounts by this bacterium. In previous studies, we have ... Thus, this bacterial urease, a major virulence factor, may also play a role in gastric carcinoma development. ... Here we have analyzed the angiogenic potential of H. pylori urease (HPU) in gastric epithelial cells. No cytotoxicity was ... Here we have analyzed the angiogenic potential of H. pylori urease (HPU) in gastric epithelial cells. No cytotoxicity was ...
... were immunized orally with 0.5-4.5 mg of Helicobacter pylori recombinant urease (rUrease) and 25-500 micrograms of Escherichia ... Immunogenicity and safety of recombinant Helicobacter pylori urease in a nonhuman primate Dig Dis Sci. 1996 Sep;41(9):1853-62. ... were immunized orally with 0.5-4.5 mg of Helicobacter pylori recombinant urease (rUrease) and 25-500 micrograms of Escherichia ...
Downloading a figure as powerpoint requires a browser with javascript support. Enable javascript and try again For help please contact [email protected] ...
... the detection limit of urease evaluated as 3 S/N was 200 ng/ml. The activity of urease enzyme depends on the presence of nickel ... The influence of accumulation time, pH of supporting electrolyte and concentration of urease on the enzyme peak height was ... Based on the results obtained the interaction of nickel(II) ions and urease can be determined using electrochemical methods. ... We were able to not only detect urease itself but also to distinguish its native and denatured form. ...
Stomach Biopsy (histology, rapid urease, culture, PCR). Biopsy tests (CLOtest, culture, histology) are done at endoscopy and ...
We show that disulfiram inhibits urease in Citrullus vulgaris (CVU), following a non-competitive mechanism, and may be one of ... The list of possible urease inhibitors is continuously increasing, with a special interest in those that interact with and ... Urease is a nickel-dependent amidohydrolase that catalyses the decomposition of urea into carbamate and ammonia, a reaction ... Inhibition of Urease by Disulfiram, an FDA-Approved Thiol Reagent Used in Humans. Ángel Gabriel Díaz-Sánchez †,* , Emilio ...
Porous silicon (PS) films prepared by electrochemical etching were used for urease... ... The present work describes the inhibition studies of free as well as immobilized urease by different heavy metals. ... Urease Immobilization Porous silicon Chromium Heavy metals Urease inhibition This is a preview of subscription content, log in ... To study the effect of other heavy metals on the interaction of urease and Cr6+, free as well as immobilized urease was ...
... and in some cases urease acts as a virulence factor, the urease phenotype has not been analyzed in the anaerobic pathogen ... Urease production was inhibited by urea and induced under growth conditions where the availability of nitrogen sources was ... This represents the first report of a plasmid-encoded urease in a gram-positive bacterium. The C. perfringens enzyme was highly ... similar to the ureases of other bacteria and cross-reacted with antibodies raised against the urease purified from Helicobacter ...
Plant ureases but not bacterial ureases possess insecticidal properties independent of its ureolytic activity. To date, the ... Urease, a nickel-dependent metalloenzyme, is synthesized by plants, some bacteria, and fungi. It catalyzes the hydrolysis of ... The active-site architecture of JBU is similar to that of bacterial ureases containing a bi-nickel center. JBU has a bound ... Crystal structure of the first plant urease from jack bean: 83 years of journey from its first crystal to molecular structure. ...