Ubiquitination, also known as ubiquitylation, is an enzymatic process that involves the bonding of an ubiquitin protein to a ... The process of ubiquitination in regulated by three main types of enzymes to take place in entirety. These include ubiquitin- ... The theory of ubiquitination involving the labeling of proteins for degradation was discovered in the late 1970s, which led to ... Ubiquitination, also known as ubiquitylation, is an enzymatic process that involves the bonding of an ubiquitin protein to a ...
BRCA1/BARD1-dependent ubiquitination of NF2 regulates Hippo-YAP1 signaling. Sachin Verma, Narayana Yeddula, Yasushi Soda, Quan ... BRCA1/BARD1-dependent ubiquitination of NF2 regulates Hippo-YAP1 signaling. Sachin Verma, Narayana Yeddula, Yasushi Soda, Quan ... BRCA1/BARD1-dependent ubiquitination of NF2 regulates Hippo-YAP1 signaling Message Subject (Your Name) has sent you a message ... BRCA1/BARD1-dependent ubiquitination of NF2 regulates Hippo-YAP1 signaling. Sachin Verma, Narayana Yeddula, Yasushi Soda, Quan ...
... Curr Opin Cell Biol. 2004 Apr;16(2):119-26. doi: 10.1016/j.ceb.2004.02.005. ...
The cells expressing the RIP1 ubiquitination mutant showed increased caspase 8 processing. Thus, ubiquitination of RIP1 appears ... RIP1 ubiquitination was blocked either by expression of a RIP mutant or by expression of dominant-negative forms of the ... Lysine 63 ubiquitination of RIP1 by TRAF2 switches RIP1 from sending a proapoptotic signal to providing a prosurvival signal. ... M. A. ODonnell, D. Legarda-Addison, P. Skountzos, W. C. Yeh, A. T. Ting, Ubiquitination of RIP1 regulates an NF-κB-independent ...
... work we review the possible cellular functions of laforin and malin with a special focus on their role in the ubiquitination of ... Lafora Disease: A Ubiquitination-Related Pathology. by Maria Adelaida García-Gimeno 1, Erwin Knecht 2,3 and Pascual Sanz 3,4,* ... Cheng, A.; Zhang, M.; Gentry, M.S.; Worby, C.A.; Dixon, J.E.; Saltiel, A.R. A role for AGL ubiquitination in the glycogen ... These changes in ubiquitination probably account for the altered distribution of the EAAT2 transporter in LD cellular models ( ...
A proteomics approach to understanding protein ubiquitination Nat Biotechnol. 2003 Aug;21(8):921-6. doi: 10.1038/nbt849. Epub ... In addition, we detected 110 precise ubiquitination sites present in 72 ubiquitin-protein conjugates. Finally, ubiquitin itself ... methodology described here provides a general tool for the large-scale analysis and characterization of protein ubiquitination ...
The Emerging Role of Linear Ubiquitination in Cell Signaling Message Subject. (Your Name) has forwarded a page to you from ... Ubiquitination is a versatile regulation mechanism: In addition to single ubiquitin molecules, chains consisting of several ...
Study Rationale: Parkinsons disease (PD) involves accumulation of toxic proteins in cells in specific areas of the brain. These cells normally have protective mechanisms to remove these toxic proteins and dysfunction of these mechanisms may play a role in the development of PD. A major goal of this grant is to explore novel ways to allow these cells to restore their ability to remove these toxic proteins.Hypothesis:Key members of the deubiquitinase (DUB) family of enzymes are thought to play a central role in the development and progression of PD by allowing the build-up of toxic proteins. This study will determine if inhibitors of these enzymes can prevent or halt the progression of PD. Study Design:The grant recipients have expertise in the identification of drugs that block the action of DUBs. The team will apply this expertise to DUB family members that are ...
Assay of Ubiquitination in Vitro.. To assay the purified VHL complex for its ability to activate HIF1α or Cln2 ubiquitination, ... 1C, HIF1α ubiquitination activity closely copurified with the VHL complex. Activation of HIF1α ubiquitination by the VHL ... 1D). In addition, the VHL complex failed to activate ubiquitination of phosphorylated Cln2, a substrate for ubiquitination ... A) An aliquot of recombinant His-HPC4-HIF1α used as substrate in the ubiquitination reactions of C and D was subjected to 8% ...
Ubiquitination of Mcl-1, that targets it for proteasomal degradation, allows for rapid elimination of the protein and ... In the last decade, a number of studies have elucidated different pathways controlling Mcl-1 ubiquitination and degradation. ... that respectively mediate and oppose Mcl-1 ubiquitination, have been formerly identified. The interaction between Mule and Mcl- ... This article belongs to the Special Issue Protein Ubiquitination) View Full-Text , Download PDF [258 KB, uploaded 8 May 2014] ...
Structure of an E6AP-UbcH7 complex: insights into ubiquitination by the E2-E3 enzyme cascade.. Huang, L., Kinnucan, E., Wang, G ... STRUCTURE OF AN E6AP-UBCH7 COMPLEX: INSIGHTS INTO THE UBIQUITINATION PATHWAY. *DOI: 10.2210/pdb1C4Z/pdb ...
Among them, histone ubiquitination has been discovered for more than three decades. However, its functions are still less well ... We will then discuss the roles of histone ubiquitination in transcription and DNA damage response and the crosstalk between ... We will then discuss the roles of histone ubiquitination in transcription and DNA damage response and the crosstalk between ... In this review, we will summarize our current understanding of histone ubiquitination and deubiquitination. In particular, we ...
Ubiquitination and proteolysis of cancer-derived mutants of Smad4 by Skp2. Xia Lin, Min Liang, Yao-Yun Liang, Katharine ... Ubiquitination and proteolysis of cancer-derived mutants of Smad4 by Skp2. Xia Lin, Min Liang, Yao-Yun Liang, Katharine ... Ubiquitination and proteolysis of cancer-derived mutants of Smad4 by Skp2. Xia Lin, Min Liang, Yao-Yun Liang, Katharine ... Ubiquitination and proteolysis of cancer-derived mutants of Smad4 by Skp2 Message Subject (Your Name) has forwarded a page to ...
Ubiquitination Is the Subject Area "Ubiquitination" applicable to this article? Yes. No. ...
Tracing down linear ubiquitination: New technology enables detailed analysis of target proteins. March 20, 2017, Goethe ... New chemistry of life: Novel ubiquitination mechanism explains pathogenic effects of Legionella infection. December 1, 2016 A ... Explore further: New chemistry of life: Novel ubiquitination mechanism explains pathogenic effects of Legionella infection ... However, target proteins of linear ubiquitination, as well as their specific cellular functions, have largely remained elusive ...
... ubiquitination. β-TrCP-IRAK1 interaction is required for K48-linked ubiquitination of IRAK1 at Lys134 and subsequent membrane- ... Inhibition of IRAK1 Ubiquitination Determines Glucocorticoid Sensitivity for TLR9-Induced Inflammation in Macrophages. Fansheng ... Inhibition of IRAK1 Ubiquitination Determines Glucocorticoid Sensitivity for TLR9-Induced Inflammation in Macrophages ... Inhibition of IRAK1 Ubiquitination Determines Glucocorticoid Sensitivity for TLR9-Induced Inflammation in Macrophages ...
The recent development of mass spectrometry methods enables qualitative and quantitative ubiquitination... ... Ubiquitination is a vital posttranslational protein modification involved in the regulation of many eukaryotic signalling ... ubiquitination tandem mass spectra validation and the time-dependent manner of CHIP ubiquitination to the reader. Conclusion: A ... Protein Ubiquitination Research in Oncology. * Authors: Mgr. Jakub Faktor; Mgr. Mariana Pjechová, Ph.D.; Prof. Ing. Lenka ...
UBE2T contributes to ubiquitination of BRCA1. A, ubiquitination of BRCA1 by UBE2T in vivo. In HEK293T cells, exogenous proteins ... Ubiquitination and Downregulation of BRCA1 by Ubiquitin-Conjugating Enzyme E2T Overexpression in Human Breast Cancer Cells. ... In vivo ubiquitination of BRCA1. HEK293T cells were transiently cotransfected with four plasmid constructs, each encoding Flag- ... Ubiquitination and proteasomal degradation of the BRCA1 tumor suppressor is regulated during cell cycle progression. J Biol ...
UBE2T contributes to ubiquitination of BRCA1. A, ubiquitination of BRCA1 by UBE2T in vivo. In HEK293T cells, exogenous proteins ... Ubiquitination and Downregulation of BRCA1 by Ubiquitin-Conjugating Enzyme E2T Overexpression in Human Breast Cancer Cells. ... Ubiquitination and Downregulation of BRCA1 by Ubiquitin-Conjugating Enzyme E2T Overexpression in Human Breast Cancer Cells ... Ubiquitination and Downregulation of BRCA1 by Ubiquitin-Conjugating Enzyme E2T Overexpression in Human Breast Cancer Cells ...
Ubiquitination plays a crucial role in regulating protein turnover. Here we show that ubiquitination regulates the stability of ... Regulation of the Stability of P-Glycoprotein by Ubiquitination. Zhigang Zhang, Jin-Ying Wu, William N. Hait and Jin-Ming Yang ... Regulation of the Stability of P-Glycoprotein by Ubiquitination. Zhigang Zhang, Jin-Ying Wu, William N. Hait and Jin-Ming Yang ... Regulation of the Stability of P-Glycoprotein by Ubiquitination. Zhigang Zhang, Jin-Ying Wu, William N. Hait and Jin-Ming Yang ...
Regulation of PAPC requires poly-ubiquitination that is dependent on phosphorylation. PAPC is phosphorylated by GKS3 in the ... We also show that precise control of PAPC by phosphorylation/ubiquitination is essential for normal Xenopus gastrulation cell ... evolutionarily conserved cytoplasmic domain, and this in turn is necessary for poly-ubiquitination by an E3 ubiquitin ligase β- ...
... also ubiquitin affinity beads and signal seeker ubiquitination kits which are comprehensive tools made from ubiquitin binding ... UBDs for the complete capture of endogenous and dynamic ubiquitinated or ubiquitylated target proteins or total ubiquitination ... which is mediated by chain specific poly-ubiquitination and/or mono-ubiquitination. Signal-Seeker™ ubiquitination tools have ... Ubiquitination Affinity Beads sku :. UBA01-beads Ubiquitination Affinity Beads (40 assays) Learn More ...
Our data show that inhibiting P-gp ubiquitination protects the transporter from degradation, and immunoprecipitation ... and immunoprecipitation experiments confirmed that PYR41 prevented P-gp ubiquitination. We further found that PYR41 treatment ... the ubiquitin-proteasome system mediates reduction of blood-brain barrier P-gp in AD and that inhibiting P-gp ubiquitination ... experiments confirmed that PYR41 prevented P-gp ubiquitination. We further found that PYR41 treatment prevented reduction of P- ...
Ubiquitination of RIP is required for tumor necrosis factor α-induced NF-κB activation. J. Biol. Chem. 281: 13636-13643. ... Ubiquitination regulates at least four steps in NF-κB pathways, including targeting IκBα for degradation, processing of NF-κB ... De-ubiquitination and ubiquitin ligase domains of A20 downregulate NF-κB signalling. Nature 430: 694-699. ... SseL inhibits NF-κB downstream of the IKK complex by impairing IκBα degradation and ubiquitination. A, IκBα degradation is ...
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Steady-state ubiquitination of TRAIL-R1 on lysine residue 273 by an endogenous machinery.A, MCF-7Casp-3 cells were transfected ... This suggested that ubiquitination of TRAIL-R1 was instrumental in its down-regulation by MARCH-8. Indeed, in cells with ... This suggested that ubiquitination of TRAIL-R1 was instrumental in its down-regulation by MARCH-8. Indeed, in cells with ... Ubiquitination by the membrane-associated RING-CH-8 (MARCH-8) ligase controls steady-state cell surface expression of tumor ...
Abstract 418: Mdm2 E3 Ligase-mediated Ubiquitination of Histone Deacetylase 1 in Vascular Calcification. Hyun Kook, Duk-Hwa ... These results demonstrate a previously unknown ubiquitination pathway as well as the involvement of HDAC1 in VC. Our results ... Here we report that MDM2-induced ubiquitination of histone deacetylase 1 (HDAC1) mediates VC. Loss of HDAC1 activity enhanced ... Calcification stresses induced MDM2 E3 ligase, which resulted in HDAC1 K74 ubiquitination. Forced expression of MDM2 enhanced ...
In vitro ubiquitination assays. Each GST-RING protein (E3) was tested against various E2s in in vitro ubiquitination assays to ... The ubiquitination pathway modifies a diverse range of proteins, thus placing protein ubiquitination at the center of numerous ... Yeast Cdc34p self-ubiquitination is thought to regulate its own levels (Skowyra et al., 1999), and the Drosophila E2 Vihar E2-C ... Self-ubiquitination of one E2, AtUBC22, was easily detected and occurred both in the presence and absence of a RING E3. Whether ...
Antigen processing: Ubiquitination & Proteasome degradation (Bos taurus) Antigen processing: Ubiquitination & Proteasome ... Antigen processing: Ubiquitination & Proteasome degradation (Canis familiaris) Antigen processing: Ubiquitination & Proteasome ... Antigen processing: Ubiquitination & Proteasome degradation (Gallus gallus) Antigen processing: Ubiquitination & Proteasome ... Antigen processing: Ubiquitination & Proteasome degradation (Oryza sativa) Antigen processing: Ubiquitination & Proteasome ...
... mono-ubiquitination, signal transduction, signal pathway and affinity beads. ... The Signal Seeker Ubiquitination enrichment Kit can purify ubiquitnated or Ubiquitylated, antibody, mono-ubiquitin, ... Using the Signal-Seeker™ Ubiquitination Detection Kit (Cat # BK161) we examined the ubiquitination of endogenous Rac1 in 3T3 ... The Ubiquitination kit contains the following components:. Lysis and protein quantitation step. IP and pre-clear step. Wash ...