Faria A, Calhau C, de F, V, Mateus N. Procyanidins as antioxidants and tumor cell growth modulators. J Agric Food Chem. 2006 ... Potential cancer-chemopreventive activities of wine stilbenoids and flavans extracted from grape (Vitis vinifera) cell cultures ... Tart cherry anthocyanins inhibit tumor development in Apc(Min) mice and reduce proliferation of human colon cancer cells. ... Pomegranate fruit extract inhibits prosurvival pathways in human A549 lung carcinoma cells and tumor growth in athymic nude ...
... cancer cell lines harbor significant levels of genetic variation, which may help explain why it can be hard to reproduce ... These individual groups of cells, typically collected from patients tumor samples and cultured to grow indefinitely in the ... And by extracting single cells and growing them in culture, the team also found that the progeny of isolated single cells could ... who leads efforts to generate cell models from patient tumors and was not involved in the study. "Weve learned that tumors ...
However, immortalization and growth of cells in culture can induce genomic changes, which can ... Cancer cell lines are widely used to research novel cancer therapeutics. ... She researched how patient data is represented by cell lines in terms of driver gene mutation patterns. ... which can alter mutation patterns in the genomes of cell lines, causing variation from patient tumors. These genetic ...
Spectral karyotyping analysis of metaphase chromosomes from primary tumor cell cultures shows that the Bcl-xl/Myc tumors ... We have isolated and characterized in vitro cultures and adoptive transfers of tumors from Bcl-xl/Myc transgenic mice. Tumors ... Gene expression profiling was used to identify differences in gene expression between tumor cells and normal plasma cells (NPC ... Genes up-regulated in group D of tumors arising from overexpression of BCL2L1 and MYC [GeneID=598;4609] in plasma cells.. ...
TILs contained >95% T cells with a considerably variable CD4/CD8 ratio. Short-term cultured autologous tumor cell lines were ... Autologous tumor cell lines are grown from corresponding tumor specimens used to isolate and expand TILs. ... Preclinical development of adoptive cell therapy with tumor-infiltrating lymphocytes for patients with renal cell carcinoma ... 33P - Preclinical development of adoptive cell therapy with tumor-infiltrating lymphocytes for patients with renal cell ...
The content of sHer2 in the supernatant of primary tumor cell cultures from breast cancer patients was determined using the ... Recombinant human sHer2 produced by HEK 293 and sHer2 isolated from the supernatant of the primary tumor cell culture from ... It should be noted that the high level of sHer2 is found in supernatants of the primary tumor cell cultures from breast cancer ... 4P - Studies of structure-functional peculiarities of soluble Her2 secreted by breast tumor cells. ...
... an international consortium that is generating novel human tumor-derived culture models, which are annotated with genomic and ... Whats needed: a Cell Line Factory to serve the community. We have launched a pilot Cell Line Factory project at the Broad ... Cell Line Factory team partnering with patients to create models from rare and underrepresented tumors. ... We will subject the primary tumor sample, matched normal tissue and associated cell lines to genome, exome or transcriptome ...
... elegans to 3D organoids and tumor spheroids, these assay systems can be challenging, expensive, lower-throughput, and/or rely ... on materials such as human primary cells that are in short supply. ... From co-cultured cells to C. elegans to 3D organoids and tumor spheroids, these assay systems can be challenging, expensive, ... Use multiple compound doses, alternate cell lines, alternate time points. It is entirely possible that running large-scale ...
... to a myofibroblast-like phenotype using 5-azacytidine do not promote tumor cell growth as efficiently as hMSCs cultured in TCM ... In cocultures of activated T cells and Hodgkin cell lines, RNAi-mediated blockade of RS cell Gal1 increased T cell viability ... The current hypothesis of the HCC cell origin includes both somatic cells (hepatocytes) and stem cells/progenitor cells. Unlike ... miRNA processing-impaired cells formed tumors with accelerated kinetics. These tumors were more invasive than control tumors, ...
Inhibition of liver tumor cell colonization in two animal tumor models by lectin blocking with D-galactose or arabinogalactan. ... Effect of sodium butyrate on carcinoembryonic antigen production by human colonic adenocarcinoma cells in culture. Cancer Res ... Tumor metastasis to the liver is more common than to other organ sites, probably due to tumor cell specificity for lectin-like ... thereby reducing tumor cell colonization of the liver and also increasing survival time of the subjects.[9-11] Pretreatment ...
Genes down-regulated in primary fibroblast cell culture at 30 min time point after infection with HCMV (AD169 strain).. ... tumor necrosis factor receptor .... 1608_at. 4233. MET. met proto-oncogene (hepatocyte .... ... Cellular mRNA levels in cytomegalovirus-infected cells were compared to the levels in cells infected with UV-inactivated virus ... By analyzing the mRNA expression profile of cells infected in the presence of cycloheximide, it was found that a minimum of 25 ...
CulturedTumor Cells, CulturedCell Line, TumorA549 CellsCaco-2 CellsHCT116 CellsHeLa Cells +Hep G2 CellsHL-60 CellsHT29 Cells ... Tumor Cells, Cultured. Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can ... Jurkat CellsK562 CellsPC12 CellsTHP-1 CellsU937 CellsEmbryonal Carcinoma Stem Cells ... All MeSH CategoriesAnatomy CategoryCellsCells, ... Cultured Tumor Cells. *Cell, Cultured Tumor. *Cells, Cultured ...
Anatomical context of Tumor Cells, Cultured. *The absence of 3,6-isoLD1 in cultured tumor cells of the central nervous system ... The successful surface expression of CD80 was obtained in both cultured tumor cell lines and primary cultured tumor cells [35]. ... Analytical, diagnostic and therapeutic context of Tumor Cells, Cultured. *We also tested fresh or newly cultured tumor cells ... These cells were lytic to cultured tumor cell lines, yet sparing normal primary cell cultures as has been reported [40]. ...
Isolation and Culture Expansion of Tumor-specific Endothelial Cells, An In Vitro System to Study Tumor Dormancy and the ... Capture and Release of Viable Circulating Tumor Cells from Blood, A Brain Tumor/Organotypic Slice Co-culture System for ... In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis, An Enzyme- and Serum-free Neural Stem Cell Culture Model for ... Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for ...
... by which some genetically deregulated and aggressive tumour cells generat ... Vascuologenesis is the de novo establishment of blood vessels and vascular networks from mesoderm-derived endothelial cell ... lined by tumour cells alone, without endothelial cells. Including examination of the cultured cell networks for PAS matrix or ... 1] in their investigation of ocular malignant melanomas, studying the tumour cells both cultured in vitro and in tumour tissue ...
Isolated tumor endothelial cells maintain specific character during long-term culture.. Matsuda K1, Ohga N, Hida Y, Muraki C, ... Increasing evidence indicates that tumor endothelial cells (TECs) are more relevant to the study of tumor angiogenesis than ... Stem cell antigen-1 was up-regulated in all four TECs, suggesting that they have a kind of stemness. Cultured TECs maintain ... However, it is challenging to isolate and culture large numbers of pure ECs from tumor tissue since the percentage of ECs is ...
Experiences with the human tumor stem cell assay(HTSCA) & its modifications for culturing gynaecological tumors. ...
If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. ... Tumor Cells, Cultured. Known as: Cultured Neoplastic Cells, Neoplastic Cells, Cultured, Neoplastic Cell, Cultured Expand. ... Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell ... Myeloid‐derived suppressor cells (MDSCs) promote tumor progression. The mechanisms of MDSC development during tumor growth ...
... expanding in culture, and freezing circulating tumor cells ... Development of an Ex Vivo Device Kit for Circulating Tumor Cell ... A Pilot Study to Facilitate Development of an Ex Vivo Device Kit for Circulating Tumor Cell Harvesting, Banking, and Apoptosis- ... stage III cancer of any solid tumor type. Patients may have been previously treated. or chemo naïve.. - Age > 18yo. - Life ...
Gastrointestinal Tumor Antigen from human cell culture for your research needs. Find product specific information including CAS ... Gastrointestinal Tumor Antigen from human cell culture ≥10,000 units/mL, buffered aqueous solution Synonym: Cancer-associated, ... Cancer Markers and Antibodies, Cancer Research, Cell Biology, Cell Signaling and Neuroscience, Immune Cell Signaling and Blood ...
... b tumor cell migration after 24 h in control culture, c tumor cell migration after 24-h incubation with L-arginine, d tumor ... 0.05-a co-culture of tumor/normal cells compared to an appropriate monoculture of normal cells. # p ≤ 0.05-a culture of tumor ... 0.05-a co-culture of tumor/normal cells compared to an appropriate monoculture of normal cells. # p ≤ 0.05-a culture of tumor ... Cell Migration Assessment. Tumor cells were plated at 5 × 105 cells/ml on 4 cm culture dishes (Nunc). After formation of ...
... has demonstrated that omega-6 fatty acids such as the fat found in corn oil promote the growth of prostate tumor cells in the ... Omega-6 fatty acids cause prostate tumor cell growth in culture; potential drug target identified By Steve Tokar ... When introduced into prostate tumor cells in culture, omega-6 fatty acid causes the production of cPLA2, which then causes the ... Working with human prostate cancer cells in tissue culture, Hughes-Fulford and her fellow researchers identified for the first ...
... and prostate tumor cell lines DU-145, LNCaP, PC-3, and HPC8L (established in our laboratory from a tumor resected from an ... Treated prostate tumor cell lines trended toward apoptosis-induction, as evident in bcl-xl/bax ratios ... cell growth inhibition assays were used to evaluate the proteins growth inhibitory activity against prostate tumor cells; and ... to inhibit prostate tumor cell growth in vitro. This study investigates the effect of eosinophil MBP extract on the expression ...
3D culture broadly regulates tumor cell hypoxia response and angiogenesis via pro-inflammatory pathways.. [Peter DelNero, ... Microarray gene expression analysis of tumor cells cultured in 2D versus 3D under ambient or hypoxic conditions revealed ... unbiased data sets describing tumor cell gene expression as a function of oxygen levels within three-dimensional (3D) culture. ... Here, we utilized alginate-based, oxygen-controlled 3D tumor models to study the interdependence of culture context and the ...
Culture of Cloned Cells from the Mouse Preputial Gland Tumor ESR 586. Toby G. Rossman, Lawrence Prutkin and Victor R. Wheatley ... Cells from the mouse preputial gland tumor ESR 586 have been cultured and cloned. Trypsin-ethylenediaminetetraacetate was used ... Culture of Cloned Cells from the Mouse Preputial Gland Tumor ESR 586 ... Culture of Cloned Cells from the Mouse Preputial Gland Tumor ESR 586 ...
Interaction of cholera enterotoxin with cultured adrenal tumor cells.. R M Wishnow, E Lifrak, C Chen ... Interaction of cholera enterotoxin with cultured adrenal tumor cells. Message Subject (Your Name) has forwarded a page to you ... In the adrenal tumor cell system ganglioside Gm1 inhibited cholera enterotoxin (CT)-induced steroidogenesis if it was ... Further studies showed that pretreatment of cultured adrenal cells with a maximal dose of CT increased cyclic adenosine 3-5- ...
... in order to track ongoing genetic mutation within a tumor. ... created a microchip device that can capture circulating tumor ... Cultured circulating tumor cells reveal genetic profile of breast cancer cells Circulating tumor cells captured with a ... Circulating tumor cells (CTCs) are living solid-tumor cells that separate from either primary or secondary tumors and go on to ... Cultured circulating tumor cells reveal genetic profile of breast cancer cells. Share this content: *facebook ...
... isolated and cultured from murine blood, demonstrate a distinct response to hypoxia compared with parental MDA-MB-231cells. (Ai ... fig3: Circulating tumour cells (CTCs), isolated and cultured from murine blood, demonstrate a distinct response to hypoxia ... fig3: Circulating tumour cells (CTCs), isolated and cultured from murine blood, demonstrate a distinct response to hypoxia ... Circulating tumour cells demonstrate an altered response to hypoxia and an aggressive phenotype. ...
These formulations generally comprise hybridoma of at least one antigen presenting cell ... Formulations comprising combinations of APCs and tumor cells and APCs and virally infected cells are disclosed. ... Day 6 dendritic cells were then used to form a co-culture with either B16 cells or 3LL cells. Each DC/tumor cell co-culture was ... here APCs and either tumor cells or virally infected cells. A co-culture of APCs and either tumor cells or virally infected ...
Adverse Cell Culture Conditions Mimicking the Tumor Microenvironment Upregulate ABCG2 to Mediate Multidrug Resistance and a ... 116 human colon cancer cell line. Reporter activity in HCT-116 cells transiently transfected with the various ABCG2 promoter ...
Adverse Cell Culture Conditions Mimicking the Tumor Microenvironment Upregulate ABCG2 to Mediate Multidrug Resistance and a ... Cell Culture and Growth Conditions. Human colon carcinoma HCT-116, and S1 and its ABCG2-overexpressing resistant S1M1-80 cell ... It is also believed to be a survival factor for cancer stem cells to drive tumor growth. Tumor microenvironment represents an ... ΔΔCt was then calculated by subtracting the ΔCt of the untreated cells (or parental cells) from the ΔCt of the treated cells ( ...
We first investigated the effects of cell source and culture conditions on lung colony formation by i.v. injected B16a (B16 ... cells and inhibition of tumor colony formation by the thromboxane A2 synthase ... The present study was undertaken to investigate the factors involved in determining the metastatic potential of cultured cells ... Phenotypic properties of cultured tumor cells: integrin alpha IIb beta 3 expression, tumor-cell-induced platelet aggregation, ...
IL-6 inhibits lipopolysaccharide-induced tumor necrosis factor production in cultured human monocytes, U937 cells, and in mice. ... IL-6 inhibits lipopolysaccharide-induced tumor necrosis factor production in cultured human monocytes, U937 cells, and in mice. ... IL-6 inhibits lipopolysaccharide-induced tumor necrosis factor production in cultured human monocytes, U937 cells, and in mice. ... IL-6 inhibits lipopolysaccharide-induced tumor necrosis factor production in cultured human monocytes, U937 cells, and in mice. ...
... and the adherence of cells to plastic were studied in cultured human A549 lung tumor cells. The density of beta AR, assayed by ... The results indicate that multiple effects of IL-1 in a cultured tumor cell line involve different mechanisms, suggesting ... IL-1 inhibited cell proliferation and thymidine uptake, and increased the adherence of A549 cells to the plastic culture flask ... and differentiation in cultured human A549 lung tumor cells.. T Nakane, T Szentendrei, L Stern, M Virmani, J Seely and G Kunos ...
A Small-Molecule Modulator of Poly-α2,8-Sialic Acid Expression on Cultured Neurons and Tumor Cells ... A Small-Molecule Modulator of Poly-α2,8-Sialic Acid Expression on Cultured Neurons and Tumor Cells ... A Small-Molecule Modulator of Poly-α2,8-Sialic Acid Expression on Cultured Neurons and Tumor Cells ... A Small-Molecule Modulator of Poly-α2,8-Sialic Acid Expression on Cultured Neurons and Tumor Cells ...
Figure 2: Induction of apoptosis in diffuse large B-cell lymphoma cell line U-2932 by etoposide treatment. Cells are labeled ... Applicability of FBS alternatives for sustainable and reproducible serum-free cell culture (a collection-related project in ... Malignant hematopoietic cell lines: in vitro models for double-hit B-cell lymphomas. Leukemia Lymphoma. 2016; 57(5):1015-20. ... Genomic Landscape of Primary Mediastinal B-Cell Lymphoma Cell Lines. PLoS One. 2015; 10(11):e0139663. ...
... and related methods of evaluating the potential responsiveness of the tumor cells and the patient to one or more therapeutic ... Provided are suspension-based cell culture systems and media for the timely and efficient proliferation of human tumor cell ... TUMOR CELL SUSPENSION CULTURES AND RELATED METHODS. Abstract:. Provided are suspension-based cell culture systems and media for ... and related methods of evaluating the potential responsiveness of the tumor cells and the patient to one or more therapeutic ...
... tumor cells must overcome glucose deprivation. Accordingly, tumor cells depend on cellular pathways promoting survival under ... As such, we sought to identify small-molecule inhibitors which sensitize tumor cells to glucose starvation by high-throughput ... Specifically, we searched for inhibitors that selectively killed tumor cells growing in glucose-free but not in normal medium. ... The efficacies of two of the identified compounds, QNZ (EVP4593) and papaverine, were validated in four different tumor cell ...
For gene expression quantification of CX 43, 32 and E-cadherin in canine mast cell tumors (MCT), 95.5% of samples were ... cell culture; breakdown for cytogenetic analysis of chromosomes (based on the Boxer breed published genome); Cell lysis for ... Animals: Thirty-six dogs with mast cell tumors. Procedures: The samples were submitted for the following analyses: stereology; ... Real Time PCR and quantification of gene expression of CX 43, 32 and E-cadherin in canine mast cells. Results: Cytogenetic ...
... breast tumor cell lines MCF-7, T-47D, and colon tumor cell line HCT-8). Insulin receptors on breast tumor cells were resistant ... The sensitivity of insulin receptors to down regulation by insulin has been measured in cultured human tumor cells ( ... Down Regulation and Dissociation of Down Regulation from Insulin Biological Action in Cultured Human Tumor Cells. Kathleen G. ... Maximum down regulation of receptors in all three types of tumor cell by prior exposure to insulin did not significantly alter ...
In three-dimensional reconstituted basement membrane culture, they undergo a well-defined program of proliferation, ... MCF-10A cells are near diploid and normal human mammary epithelial cells. ... Three-dimensional Cell Culture MCF-10A, MCF-10AT, and MCF-10CA1a cells were cultured on plastic tissue culture dishes (Nalge ... more relevant systems in which to elucidate changes in cell growth, cell: cell adhesion, cell: cell junctions, and cell: extra- ...