They degrade polypeptides and are distinguished by their substrate specificities. The cathepsin family consists of 12 cysteine ... Component A: Cathepsin B substrate, Ex/Em=354 nm/442 nm upon cleavage: 1 mM, 50 µL Component B: AMC, Fluorescence Reference ...

Substrate specificity of acyl-lipid Δ9-desaturase from Prochlorothrix hollandica cyanobacterium producing myristoleic acid A. ... Substrate Specificity of Acyl-Lipid Δ9-Desaturase from Cyanobacterium sp. IPPAS B-1200, a Cyanobacterium with Unique Fatty Acid ...

... of human embryonic stem cells and derived cell types stabilized by substrate stiffness regulation promotes lineage specificity ... reflects the fundamental mechanical property of live cells measured by atomic force microscopy and is determined by substrate ...

The initial oxidation of 1,1-DCE is believed to be catalysed by methane monooxygenase, which has a broad substrate specificity ... Both substrates are activated by CYP2E1. Thus, there is a potential for competitive inhibition when simultaneous exposure to ... There was, however, no evidence of competitive inhibition when exposure to both substrates was 400 mg/m3 or less. As ... 1-DCE and other substrates for CYP2E1 (e.g., p-nitrophenol and chlorozoxazone). The researchers also showed that different ...

Exploring ABOBEC3A and APOBEC3B substrate specificity and their role in HPV positive head and neck cancer.Papini C, Wang Z, ... Erratum: Exploring APOBEC3A and APOBEC3B substrate specificity and their role in HPV positive head and neck cancer.Papini C, ... Insights into DNA substrate selection by APOBEC3G from structural, biochemical, and functional studies.Ziegler SJ, Liu C, ... OLE RNA, an RNA motif that is highly conserved in several extremophilic bacteria, is a substrate for and can be regulated by ...

On the other hand, the PLB showed substrate specificity and required metal ions for alkaline activity.. The cDNA sequence of ... Carbohydrate Specificity of Lectins from Boletopsis leucomelas and Aralia cordate. Yu KOYAMA, Takuji SUZUKI, Shoji ODANI, ... The carbohydrate specificity of three novel lectins, Boletopsis leucomelas lectin (BLL), Aralia cordate lectin (ACL), and ... At a concentration of 7.5% (w/v) NaCl, both proteases preferred animal proteins to vegetable proteins as natural substrates. In ...

For example, bacterial enzymes are highly specialized devices, with very narrow substrate specificities. Thousands of different ... have high specificity and relatively few side effects. Carefully tailored cancer vaccines under development in the late 1990s ... specificity of action....Designed smart pharmaceuticals might activate themselves only when, where, and if needed." ... is the rather limited range of molecular substrates they can utilize. ...

The double bonds in the meromycolate chain are then modified resulting in the exhibition of exquisite substrate specificity (14 ... Moreover, the substrate similarity for the active sites of these enzymes points to the possibility of one potential inhibitor ...

Total chemical synthesis of a D-enzyme: the enantiomers of HIV-1 protease show reciprocal chiral substrate specificity [ ... and antihapten specificity is injected in the first phase. In the second phase a radiolabeled hapten is administered, which is ...

"Structural rationale for the short branched substrate specificity of the glycogen debranching enzyme GlgX". Proteins. 78 (8): ... May 2008). "Substrate recognition mechanism of alpha-1,6-glucosidic linkage hydrolyzing enzyme, dextran glucosidase from ... Their active sites are selective towards the respective substrates, ensuring proper activation of GDE. Besides the active sites ... Gillard BK, Nelson TE (September 1977). "Amylo-1,6-glucosidase/4-alpha-glucanotransferase: use of reversible substrate model ...

Crystal structure of human dipeptidyl peptidase IV in complex with a decapeptide reveals details on substrate specificity and ... Structural and kinetic analysis of the substrate specificity of human fibroblast activation protein alpha, JOURNAL OF ... The structure gives an increased understanding of the membrane, substrate and reductase binding surfaces, and has enabled the ...

... enzymes and catalytic antibodies have high substrate specificity. However, they can be costly to engineer. L-proline, an amino ...

Studies on the substrate specificity of a carboxyl ester hydrolase from human pancreatic juice. I. Action on carboxyl esters, ... Effects of the short-chain triglyceride triacetin on intestinal mucosa and metabolic substrates in rats. Lynch, J.W., Miles, J. ... Fluorescence spectroscopic characterization of Humicola lanuginosa lipase dissolved in its substrate. Jutila, A., Zhu, K., ... We have therefore used triacetin as a substrate and compared out method with potentiometry (pH-stat) and spectrophotometry [23] ...

The different substrate specificities of the Zea mays dihydroflavonol 4- reductase paralogs A1 and A1* are determined by few ... Re-investigating substrate specificity of dihydroflavonol 4-reductase with respect to the B-ring hydroxylation pattern of ... Re-investigating substrate specificity of dihydroflavanol 4-reductase with respect to the B-ring hydroxylation pattern of ... Substrate specificity of polyphenol oxidases of apple shed new light on their physiological role; Vortrag: XXIXth ...

1955) A simple model of molecular specificity in enzyme-substrate systems. II. The correlation of the michaelis constant with ... 1955) A simple model of molecular specificity in enzyme-substrate systems. I. Theory and applications to the system ... acetylcholinesterase-substrate Journal of the American Chemical Society. 77: 1966-1972. Bernhard SA, Garfield E, Hammett LP. ( ...

Changing the substrate specificity of arogenate dehydratases (ADTs) from Arabidopsis thaliana., Megan ES Smith-Uffen ...

Deep profiling of protease substrate specificity enabled by dual random and scanned human proteome substrate phage libraries. ... Broad identification of optimal cleavage sites and natural substrates of proteases is critical for drug discovery and to ... Here, we present a method that employs two genetically encoded substrate phage display libraries coupled with next generation ... Furthermore, the enhanced resistance in lht1 could be attributed to a specific deficiency of its main physiological substrate, ...

Substrate specificity and enzyme inducibility in the presence of human cervical mucins have not been determined, nor is there ... Corfield AP, Michalski J-C, Schauer R. The substrate specificity of sialidases from micro-organisms and mammals. Perspectives ... Sialidase substrates-sialic (neuraminic) acids. Sialic acids are acidic nine carbon sugars, which frequently occupy the non- ... T vaginalis also expresses a sialidase with shows specificity for α2-3 linked sialic acids, but which is unable to liberate α2- ...

Enzyme Active Site and Substrate Specificity. The chemical reactants to which an enzyme binds are the enzymes substrates. ... The enzyme-substrate complex can lower the activation energy by contorting substrate molecules in such a way as to facilitate ... Enzymes and substrates are thought to bind with an induced fit, which means that enzymes and substrates undergo slight ... Enzymes bind to substrates and catalyze reactions in four different ways: bringing substrates together in an optimal ...

The activity of bromelain is influenced by various factors, including temperature, pH, substrate specificity, and the presence ...

Dephosphorylates the 5 and 2(3)-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate ... Supplies the nucleotide substrate for thymidylate synthetase. Gene Name:. DCTD. Uniprot ID:. P32321 Molecular weight:. 21013.96 ... This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. Gene Name: ...

Dopamine Is a Low-Affinity and High-Specificity Substrate for the Human UDP-Glucuronosyltransferase 1A10 Katriina Itäaho, ... Evaluation of specificities in the in vitrometabolism of therapeutic agents by human liver microsomes. ... Mechanistic Analysis of the Inactivation of Cytochrome P450 2B6 by Phencyclidine: Effects on Substrate Binding, Electron ... DRUG-DRUG INTERACTIONS FOR UDP-GLUCURONOSYLTRANSFERASE SUBSTRATES: A PHARMACOKINETIC EXPLANATION FOR TYPICALLY OBSERVED LOW ...

Fernie, A. R.; Toth, S. Z.: Identification of the Elusive Chloroplast Ascorbate Transporter Extends the Substrate Specificity ...

Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases ... The MCU inhibitor Ru360 was used to ensure MCUC-dependent mCa2+ uptake specificity. Cytosolic Ca2+ (cCa2+) transients were ... Reduced cardiac efficiency and altered substrate metabolism precedes the onset of hyperglycemia and contractile dysfunction in ... The switch from one energy substrate to another is regulated by metabolic signals that act as allosteric regulators of key ...

These systems therefore solve the specificity problem by possessing such broad substrate specificities that they metabolise ... substrate specificity, and MRP2-mediated drug resistance". Biochim. Biophys. Acta 1461 (2): 377-94. PMID 10581368.. ... In phase I a variety of enzymes act to introduce reactive and polar groups into their substrates. One of the most common ... The actions of some drugs as substrates or inhibitors of enzymes involved in xenobiotic metabolism are a common reason for ...

Substrate Specificity. To determine the substrate specificity of enzyme, glucose-1-phosphate, glucose, fructose and galactose-6 ... Substrate Specificity. Substrate specificity of purified glucose-6-phosphate dehydrogenase was observed by using different ... Effect of pH on the Km and Vmax values of substrate has been investigated in the pH range 7.5 - 9.0. The sub- strate ... Effect of Substrate and Coenzyme Concentration. Effect of substrate concentration on purified glucose-6-phosphate dehydrogenase ...

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade ... CYP450 2C9 substrate. Non-substrate. 0.7624. CYP450 2D6 substrate. Non-substrate. 0.8025. ...

Deciphering the Substrate Specificity of SbnA, the Enzyme Catalyzing the First Step in Staphyloferrin B Biosynthesis. ...

25 Lang, R, Kocourek,A, Braun, M,Tschesche, H, Huber R, Bode,W, Maskos, K (2001). Substrate specificity determinants of human ...

The substrate specificity of hormone-sensitive lipase from adipose tissue of the Antarctic fish Trematomus newnesi J. R. Hazel, ... View articletitled, The substrate specificity of hormone-sensitive lipase from adipose tissue of the Antarctic fish ,em, ...