Sterol 14-demethylase - Wikipedia
... lanosterol 14alpha-demethylase, and sterol 14alpha-demethylase. This enzyme participates in biosynthesis of steroids. As of ... In enzymology, a sterol 14-demethylase (EC 188.8.131.52) is an enzyme that catalyzes the chemical reaction obtusifoliol + 3 O2 + ... The systematic name of this enzyme class is sterol,NADPH:oxygen oxidoreductase (14-methyl cleaving). Other names in common use ... Aoyama Y, Yoshida Y (1991). "Different substrate specificities of lanosterol 14a-demethylase (P-45014DM) of Saccharomyces ...https://en.wikipedia.org/wiki/Sterol_14-demethylase
AID 516340 - Binding affinity to Trypanosoma cruzi sterol 14-alpha-demethylase by UV-Spectrophotometry - PubChem
Binding affinity to Trypanosoma cruzi sterol 14-alpha-demethylase by UV-Spectrophotometry. ...https://pubchem.ncbi.nlm.nih.gov/bioassay/516340
Prothioconazole and Prothioconazole-Desthio Activities against Candida albicans Sterol 14-α-Demethylase | Applied and...
Sterol 14α-demethylase inhibitors (DMIs) are a class of antifungal compounds used to treat both agricultural and medical fungal ... Sterol extraction and analysis.Nonsaponifiable lipids were extracted as reported previously (16). Samples were dried in a ... Prothioconazole and Prothioconazole-Desthio Activities against Candida albicans Sterol 14-α-Demethylase. Josie E. Parker, ... Azole binding properties of Candida albicans sterol 14-alpha demethylase (CaCYP51). Antimicrob. Agents Chemother. 54:4235-4245. ...https://aem.asm.org/content/79/5/1639?ijkey=05f00f5a6dc5c26989f330b78a9e709023afab96&keytype2=tf_ipsecsha
RCSB PDB - 5TZ1: Crystal structure of sterol 14-alpha demethylase (CYP51) from Candida albicans in complex with the...
Structural analyses of Candida albicans sterol 14 alpha-demethylase complexed with azole drugs address the molecular basis of ... Sterol 14-alpha demethylase A, B 490 Candida albicans EC#: 184.108.40.206 IUBMB Mutation: A48P, S263L Gene Name(s): ERG11 CYP51 ... Crystal structure of sterol 14-alpha demethylase (CYP51) from Candida albicans in complex with the tetrazole-based antifungal ... azole-mediated inhibition of fungal sterol biosynthesis. ...http://www.rcsb.org/pdb/explore/explore.do?structureId=5TZ1
RCSB PDB - 1E9X: Cytochrome P450 14 alpha-sterol demethylase (CYP51) from Mycobacterium tuberculosis in complex with...
Sterol Demethylase (Cyp51) from Mycobacterium Tuberculosis in Complex with Azole Inhibitors ... Cytochrome P450 14 alpha-sterol demethylase (CYP51) from Mycobacterium tuberculosis in complex with 4-phenylimidazole. ...http://www.rcsb.org/pdb/explore/materialsAndMethods.do?structureId=1E9X
Sterol 14α-demethylase mutation leads to amphotericin B resistance in Leishmania mexicana
... ... Expression of wild-type sterol 14α-demethylase in the resistant cells caused reversion to drug sensitivity and a restoration of ... Metabolomic analysis demonstrated that ergosterol, the sterol known to bind the drug, is prevalent in wild-type cells, but ... sterol 14α-demethylase (CYP51). The mutation, N176I, is found outside of the enzyme's active site, consistent with the fact ...https://research-repository.st-andrews.ac.uk/handle/10023/11159
A Point Mutation in the 14α-Sterol Demethylase Gene cyp51A Contributes to Itraconazole Resistance in Aspergillus fumigatus |...
The genes encoding 14α-sterol demethylases (cyp51A and cyp51B) were analyzed in 12 itraconazole (ITC)-resistant and three ITC- ... A Point Mutation in the 14α-Sterol Demethylase Gene cyp51A Contributes to Itraconazole Resistance in Aspergillus fumigatus. T. ... Recently, we have described the existence of two genes coding for two different 14α-demethylases in A. fumigatus, cyp51A and ... The target of the azole antifungals is 14α-demethylase, and alterations in the sequence of the gene encoding this enzyme (ERG11 ...https://aac.asm.org/content/47/3/1120?ijkey=63af819b77421c0a32d5425f00f0b31338670682&keytype2=tf_ipsecsha
Differences in Interactions between Azole Drugs Related to Modifications in the 14-α Sterol Demethylase Gene (cyp51A) of...
Genetic control of resistance to the sterol 14α-demethylase inhibitor fungicide prochloraz in the cereal eyespot pathogen ... and the use of demethylase inhibitors as pesticides in agriculture (8, 11, 12, 27). ... The description of two different 14-α sterol demethylase-like genes (cyp51A and cyp51B) in Aspergillus spp. (19) opens the ... In vitro drug interaction was evaluated as described earlier (1, 14, 18) with a two-dimensional, two-agent broth microdilution ...https://aac.asm.org/content/49/5/2119?ijkey=a9587d7d6f0974e39f40b088e46022c339ae761c&keytype2=tf_ipsecsha
Complexes of Trypanosoma cruzi Sterol 14 -Demethylase (CYP51) with Two Pyridine-based Drug Candidates for Chagas Disease:...
Azole inhibitors of sterol 14α-demethylase (CYP51) were proven effective against Chagas, and antifungal drugs posaconazole and ... 2013) Complexes of Trypanosoma cruzi Sterol 14 -Demethylase (CYP51) with Two Pyridine-based Drug Candidates for Chagas Disease ... Complexes of Trypanosoma cruzi Sterol 14 -Demethylase (CYP51) with Two Pyridine-based Drug Candidates for Chagas Disease: ... The binding parameters and inhibitory effects on sterol 14α- demethylase activity in reconstituted enzyme reactions confirmed ...http://researchrepository.murdoch.edu.au/id/eprint/19447/
Azole fungicides affect mammalian steroidogenesis by inhibiting sterol 14 α-demethylase and aromatase - Research Collection
Meiosis-activating sterols; Sterol 14 alpha-demethylase; Testis meiosis-activating sterol (T-MAS) ... Azole fungicides affect mammalian steroidogenesis by inhibiting sterol 14 α-demethylase and aromatase. * Mendeley ... Aromatase; CYP19; CYP51; Endocrine disruption; Follicle fluid meiosis-activating sterol (FF-MAS); Lanosterol; ...https://www.research-collection.ethz.ch/handle/20.500.11850/56685
Iron in the structure of Crystal Structure of Sterol 14-Alpha Demethylase (CYP51) From Trypanosoma Brucei in Ligand Free State ...
Crystal Structure of Sterol 14-Alpha Demethylase (CYP51) From Trypanosoma Brucei in Ligand Free State ... The binding sites of Iron atom in the structure of Crystal Structure of Sterol 14-Alpha Demethylase (CYP51) From Trypanosoma ... Iron in the structure of Crystal Structure of Sterol 14-Alpha Demethylase (CYP51) From Trypanosoma Brucei in Ligand Free State ...http://iron.atomistry.com/pdb3g1q.html
Iron in the structure of Cytochrome P450 14 Alpha-Sterol Demethylase (CYP51) From Mycobacterium Tuberculosis in Complex With 4...
Cytochrome P450 14 Alpha-Sterol Demethylase (CYP51) From Mycobacterium Tuberculosis in Complex With 4-Phenylimidazole ... The binding sites of Iron atom in the structure of Cytochrome P450 14 Alpha-Sterol Demethylase (CYP51) From Mycobacterium ... Iron in the structure of Cytochrome P450 14 Alpha-Sterol Demethylase (CYP51) From Mycobacterium Tuberculosis in Complex With 4- ...http://iron.atomistry.com/pdb1e9x.html
ERG11 - Lanosterol 14-alpha demethylase - Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) ...
It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol (By similarity). ... Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism. ... "In vitro and in vivo effects of 14alpha-demethylase (ERG11) depletion in Candida glabrata.". Nakayama H., Nakayama N., Arisawa ... "Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and ...https://www.uniprot.org/uniprot/P50859
Bio-crude transcriptomics: Gene discovery and metabolic network reconstruction for the biosynthesis of the terpenome of the...
A curated contig for a putative sterol esterase (LIPA, E.C. 220.127.116.11) may take part in the esterification of sterols with long- ... nor did they methylate common plant sterols in spite of harbouring apparently canonical sterol binding domains. None of the six ... sterol 24-C-methyltransferase; ERG25, methylsterol monooxygenase; CPI1, cycloeucalenol cycloisomerase; CYP51, sterol 14- ... demethylase; ERG24, Δ-14-sterol reductase; EBP, cholestenol Δ-isomerase; MSMT, 24-methylenesterol C-methyltransferase; SC5DL, ...https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-13-576
Keystone Symposia | Scientific Conferences on Biomedical and Life Science Topics
Sterol 14-Demethylase Inhibitors for Chagas Disease. Andrew P. Waters, University of Glasgow, UK Commitment to sexual ...https://www.keystonesymposia.org/index.cfm?e=web.Meeting.Program&meetingid=1163
... sterol 14-demethylase activity,IEA; GO:0016020,membrane,IEA; GO:0016126,sterol biosynthesis,IEA; GO:0016491,oxidoreductase ... 1e-14. 23% (70/293). yhr053c Metallothionein precursor related cluster. 365. 3e-34. 100% (61/61). GO:0005507,copper ion binding ... 3e-14. 51% (45/88). Translation, ribosomal structure and biogenesis GO:0003743,translation initiation factor activity,IDA; GO: ... 3.4.14.-. Amino acid transport and metabolism GO:0000329,vacuolar membrane (sensu Fungi),IDA; GO:0003824,catalytic activity,IEA ...http://megasun.bch.umontreal.ca/Software/Scerevisiae_PEDANT/S_cerevisiae.fasta.html
Sterol Biosynthesis in Trypanosomatid Parasites. Frederick Buckner; Fiscal Year: 2004. ..The drugs discovered in this research ... Targeting Trypanosoma cruzi sterol 14α-demethylase (CYP51). Galina I Lepesheva. Department of Biochemistry School of Medicine, ... Sterol Biosynthesis in Trypanosomatid Parasites. Frederick Buckner; Fiscal Year: 2004. *High Throughput Assay for Screening ... Sterol 14-demethylase inhibitors for Trypanosoma cruzi infections. Frederick S Buckner. Department of Medicine, University of ...http://www.labome.org/topics/chemicals/chemical/pharmacologic/therapeutic/anti/antiparasitic/antiprotozoal/trypanocidal-agents-16003.html
Drug Targets in Kinetoplastid Parasites | Springer for Research & Development
Sterol 14-Demethylase Inhibitors for Trypanosoma cruzi Infections Frederick S. Buckner. Pages 61-80 ...https://rd.springer.com/book/10.1007%2F978-0-387-77570-8
Diverse Inhibitor Chemotypes Targeting Trypanosoma cruzi CYP51
A validated therapeutic target in T. cruzi is CYP51, an essential enzyme in the sterol biosynthesis pathway. We report results ... 2010) Structural insights into inhibition of sterol 14alpha-demethylase in the human pathogen Trypanosoma cruzi. J Biol Chem ... Sterols Is the Subject Area "Sterols" applicable to this article? Yes. No. ... Inhibition of sterol biosynthesis by GC-MS analysis was confirmed for all individually acquired hits. The major sterol observed ...https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0001736
Analysis of ergosterol and gene expression profiles of sterol Δ5,6-desaturase (ERG3) and lanosterol 14α-demethylase (ERG11) in...
Analysis of ergosterol and gene expression profiles of sterol Δ5,6-desaturase (ERG3) and lanosterol 14α-demethylase (ERG11) in ...http://herbmedpharmacol.com/citation_report/jhp-1229/crossref
CYP51A1 elisa kit | Human cytochrome P450, family 51, subfamily A, polypeptide 1 ELISA Kit-NP 000777.1
Endogenous Sterols Pathway antibodies. Endogenous Sterols Pathway Diagram. Metabolism Pathway antibodies. Metabolism Pathway ... Molecular Function: sterol 14-demethylase activity; iron ion binding; heme binding. Biological Process: xenobiotic metabolic ... CYPLI; Cytochrome P450 51A1; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Sterol 14-alpha demethylase. ... sterol 14-alpha demethylase; cytochrome P450; family 51; subfamily A; polypeptide 1 ...https://www.mybiosource.com/prods/ELISA-Kit/Human/cytochrome-P450-family-51-subfamily-A-polypeptide-1/CYP51A1/datasheet.php?products_id=9341895
KEGG SSDB Best Search Result: ure:UREG 00168
tcc:18608823 sterol 14-demethylase K05917 486 109 ( 4) 31 0.314 102 -, 3 aaf:AURANDRAFT_10275 hypothetical protein 724 108 ( 4 ... cot:CORT_0G04140 Erg5 C-22 sterol desaturase K09831 524 103 ( -) 29 0.308 65 -, 1 dgi:Desgi_3800 2-nitropropane dioxygenase- ... clus:A9F13_04g02805 putative C-22 sterol desaturase K09831 524 122 ( -) 34 0.323 65 -, 1 rpf:Rpic12D_4372 putative cytochrome ... acan:ACA1_073430 sterol C22 desaturase -like, putative K09832 494 109 ( -) 31 0.317 63 -, 1 ccx:COCOR_06417 cytochrome P450 109 ...http://www.kegg.jp/ssdb-bin/ssdb_best?org_gene=ure:UREG_00168
Functional Characterization and Localization of Pneumocystis carinii Lanosterol Synthase
Putative P. carinii sterol pathway. A putative sterol biosynthetic pathway indicating genes that have been cloned and ... The most abundant sterol found in Pneumocystis is cholesterol, which accounts for 81% of its total sterols (15). It is ... the first sterol intermediate of the mammalian and fungal sterol biosynthetic pathways. During this conversion, Erg7p performs ... indicating that lipid particles may function not only in sterol biosynthesis but may also help to regulate the flux of sterols ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2805306/
EC 18.104.22.168 sterol 14α-demethylase. EC 22.214.171.124 3,6-diketocamphane 1,2-monooxygenase. EC 126.96.36.199 tryptophan N- ... EC 1.14.14. With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen. Contents. EC 188.8.131.52 ... EC 184.108.40.206 (13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine 8-hydroxylase EC 220.127.116.11 germacrene A acid 8β-hydroxylase ... EC 18.104.22.168 methyltetrahydroprotoberberine 14-monooxygenase. EC 22.214.171.124 protopine 6-monooxygenase. EC 126.96.36.199 (S)- ...https://www.qmul.ac.uk/sbcs/iubmb/enzyme/EC1/14/14/
Viridin - Wikipedia
ISBN 978-0-470-74167-2. Hanson, James R.; O'Leary, Margaret A.; Wadsworth, Harry J. "Fungal cleavage of the sterol side chain ... Loss of a hydrogen atom from C-15 during removal of the C-14 methyl group suggests a pathway similar to that of other steroid ... Analogous to the cholesterol pathway, the C-14 methyl group is lost as formic acid by action of sterol 14-demethylase. Though ... the loss of C-13 and C-14 methyl groups, aromatization of the C-ring, removal of the side chain, and oxidation of the A-ring. ...https://en.wikipedia.org/wiki/Viridin