... and immune cell infiltration levels. Finally, the interaction between HNK and two HNK-targets regulating the cell cycle was ... such as inducing cell cycle arrest, inhibiting metastasis, and overcoming immunotherapy resistance in breast cancer cells. ... However, the molecular mechanisms of action of HNK in BCSCs, as well as its effects on the cell cycle, remain unclear. This ... BCSCs are mostly dormant, and therefore, activating them and modulating the cell cycle are important for successful therapy ...

Cycling Stem Cells Are Radioresistant and Regenerate the Intestine.. Sheng X, Lin Z, Lv C, Shao C, Bi X, Deng M, Xu J, Guerrero ... Cycling Stem Cells Are Radioresistant and Regenerate the Intestine. Cell Rep. 2020;32(4):107952. ... The ISSCR Releases Global Standards to Enhance Rigor and Reproducibility of Stem Cell Research ... Columbia Stem Cell Initiative Stem Cell Core Facility. *Harvard Stem Cell Institute iPS Core Facility ...

For cell cycle synchronization T98G cells were rendered quiescent by serum starvation for 48 h and then released into the cell ... From late mitosis to the G1 phase of the cell cycle. * Post author By exposed ... From late mitosis to the G1 phase of the cell cycle ORC CDC6 and Cdt1 form the machinery necessary to load MCM2-7 complexes ... EXPERIMENTAL PROCEDURES Cell Culture and Synchronization HEK293T T98G and HeLa cells were produced in Dulbeccos altered ...

Unfractionated Bulk Culture of Mouse Skeletal Muscle to Recapitulate Niche and Stem Cell Quiescence… ... Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment ... Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment ... Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment ...

Embryonic stem cells (ESCs) present a fast cell cycle with a short G1 phase. This is due to the lack of expression of cell ... Embryonic stem cells (ESCs) present a fast cell cycle with a short G1 phase. This is due to the lack of expression of cell ... Understanding the mechanisms regulating cell cycle, proliferation and potency of pluripotent stem cells guarantees their safe ... Understanding the mechanisms regulating cell cycle, proliferation and potency of pluripotent stem cells guarantees their safe ...

... known as glioma stem-like cells (GSCs), are thought to play a major role in tumor relapse due to their stem cell-like ... Cell cycle distribution was analyzed by using the univariate cell cycle platform in Flow Jo V10 software, the Den Jett Fox ... Kreso, A. & Dick, J. E. Evolution of the cancer stem cell model. Cell Stem Cell 14, 275-291. https://doi.org/10.1016/j.stem. ... known as glioma stem-like cells (GSCs), are thought to play a major role in tumor relapse due to their stem cell-like ...

... and ID1/3 in driving cancer stem cell traits in aggressive breast cancer. ... ZNF148 suppressed cell proliferation and migration and was transcriptionally repressed by MYC in breast cancer. Depletion of ... Silencing of ZNF148 increased the stemness and tumorigenicity in TNBC cells. These findings uncover a previously unknown tumor ... Global transcriptome and chromatin occupancy analyses of ZNF148 revealed a central role in inhibiting cancer cell de- ...

In addition, the growth inhibitory effect of MNBE was specific to cervical cancer cells than normal cells. Cell cycle arrest ... The growth-inhibiting effect of MNBE was further confirmed by performing cell cycle analysis and apoptosis assay using flow ... significantly suppressed the growth and survival of cervical cancer cells in a dose-dependent manner by inducing cell cycle ... and ME-180 cell lines. Cytotoxic effect of MNBE on cultured cell lines was evaluated by MTT and clonogenic assay. ...

Sustained PU.1 Levels Balance Cell-Cycle Regulators to Prevent Exhaustion of Adult Hematopoietic Stem Cells. In: Molecular Cell ... Sustained PU.1 Levels Balance Cell-Cycle Regulators to Prevent Exhaustion of Adult Hematopoietic Stem Cells. Molecular Cell. ... Sustained PU.1 Levels Balance Cell-Cycle Regulators to Prevent Exhaustion of Adult Hematopoietic Stem Cells. Molecular Cell, 49 ... Sustained PU.1 Levels Balance Cell-Cycle Regulators to Prevent Exhaustion of Adult Hematopoietic Stem Cells, Molecular Cell, ...

Hirao A, Arai F, Suda T (December 2004). "Regulation of cell cycle in hematopoietic stem cells by the niche". Cell Cycle. 3 (12 ... Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. Stem cells can ... 2005). "Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors". Stem Cells. 23 (6 ... "Integrins are markers of human neural stem cells". Stem Cells. 24 (9): 2078-84. doi:10.1634/stemcells.2005-0595. PMID 16690778 ...

... neurotransmitter transport/synaptic vesicle cycle, cytoskeleton organization, and cell stress response, demonstrating ... To better understand RABV pathogenesis in humans, we generated human-induced pluripotent stem cell (hiPSC)-derived neurons to ... offer the opportunity for an inimitable study of RABV infection at a molecular level in a pathologically relevant cell type. ... obtained from infected primary neurons or mouse brain samples are more relevant to human clinical rabies than permissive cell ...

... cell cycle, and inflammatory response in the diseased area. Collectively, our results suggest that ASC-exosomes effectively ... Previously, we showed that exosomes derived from human adipose tissue-derived mesenchymal stem cells (ASC-exosomes) attenuate ... restore epidermal barrier functions in AD by facilitating the de novo synthesis of ceramides, resulting in a promising cell- ... Mesenchymal stem cells (MSCs) are multipotent stem cells that reside in adult tissues. Because MSCs can differentiate into many ...

... the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, ... A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. In this paper, we describe ... Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. ... "Cell-cycle restriction limits DNA damage and maintains self-renewal of leukaemia stem cells," Nature, vol. 457, no. 7225, pp. ...

Life of the B10 mouse: a view from the hair follicles and tissue stem cells: Hair follicles and tissue stem cells in the B10 ... Cell Cycle 5: 274-278.. 43.. Sorensen, E.B., S.D. Conner (2010) Gamma secretase-dependent cleavage initiates Notch signaling ... Noggle, S.A., D. Weiler, B.G. Condie (2006) Notch signaling is inactive but inducible in human embryonic stem cells. Stem Cells ... Chiba, S. (2006) Notch Signaling in Stem Cell Systems. Stem Cells 24: 2437-2447. ...

The intensities of canonical senescence biomarkers integrate the duration of cell-cycle withdrawal July 28, 2023 ... Telomere length maintenance ensures self-renewal of human embryonic stem cells (hESCs) and induced pluripotent stem cells ( ... A balance between elongation and trimming regulates telomere stability in stem cells. ... suggesting heightened sensitivity of stem cells to replication stress at overly long telomeres. Thus, tight control of telomere ...

Pharmacological Actions : Antiproliferative , Cell cycle arrest, Proteasome Inhibitors. Additional Keywords : Cancer Stem Cells ... Brain Cancer Stem Cells, Breast Cancer Stem Cells, Cancer Stem Cells, Colon Cancer Stem Cells, Liver Cancer Stem Cells, Lung ... Brain Cancer Stem Cells, Breast Cancer Stem Cells, Cancer Stem Cells, Colon Cancer Stem Cells, Liver Cancer Stem Cells ... Cancer Stem Cell, Nasopharyngeal Cancer Stem Cells, Ovarian Cancer Stem Cell, Pancreatic Cancer Stem Cell, Prostate Cancer Stem ...

Charafe-Jauffret E, Ginestier C, Birnbaum D. Cancer stem cells: just sign here! Cell Cycle. 2010;9:229-230. [PubMed] [DOI] [ ... Breast cancer stem cells-from origins to targeted therapy. Stem Cell Investig. 2017;4:96. [PubMed] [DOI] [Cited in This Article ... ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome. Cell Stem Cell. ... Based on the stem cell hypothesis, cancer might arise from a cell population with the stem property of self-renewal. Such a ...

... published by Haruko Obokata in the scientific journal Nature in January detailed simple ways to reprogramme mature animal cells ... Ambassadors, diplomatic representatives cycle in Hanoi to mark ASEAN Family Day Society ... Japanese researcher stands by her disputed stem cell discovery. Wednesday, April 09, 2014, 17:30 GMT+7 ... a Japanese researcher under fire for fabricating parts of scientific papers hailed as breakthroughs in stem cell research said ...

Cao, B. and Huard, J. (2004) Muscle-Derived Stem Cells. Cell Cycle, 3, 104-107. http://dx.doi.org/10.4161/cc.3.2.644 ... Wu, Y. and Zhao, R.C. (2012) The Role of Chemokines in Mesenchymal Stem Cell Homing to the Myocardium. Stem Cell Reviews, 8, ... Is Not a Major Determinant for the Success of Stem Cell Therapy-Cross-Talk between Muscle-Derived Stem Cells and Host Cells. ... Clonal Analysis of Synovial Fluid Stem Cells to Characterize and Identify Mesenchymal Stromal Cell/Mesenchymal Progenitor Cell ...

... and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). Our ... We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a ... We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a ... which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. Here, we describe ...

Predicting stem cell fate changes by differential cell cycle progression patterns. M. Roccio; D. Schmitter; M. Knobloch; Y. ... Conditional mutation of Rb causes cell cycle defects without apoptosis in the central nervous system. D. MacPherson; J. Sage; D ... Fast Detection of Cells Using a Continuously Scalable Mexican-Hat-Like Template. K. Chaudhury; Z. Püspöki; A. Muñoz Barrutia; D ... Autonomous and self-sustained circadian oscillators displayed in human islet cells. P. Pulimeno; T. Mannic; D. Sage; L. ...

We found that HSCs from Lnk-/- mice have an increased quiescent fraction, decelerated cell cycle kinetics, and enhanced ... negative regulator of JAK2 in stem cells and TPO/Mpl/JAK2/Lnk as a major regulatory pathway in controlling stem cell self- ... Lnk controls mouse hematopoietic stem cell self-renewal and quiescence through direct interactions with JAK2. ... Lnk controls mouse hematopoietic stem cell self-renewal and quiescence through direct interactions with JAK2. ...

Brain sensitivity to insulin may be modulated by menstrual cycle. Sep 22, 2023 ... Stem cells give rise to every tissue and organ in the body. Satellite stem cells are specialized muscle stem cells that live in ... The research, to be published in the June 5 issue of Cell Stem Cell, shows for the first time that a protein called Wnt7a ... Director of Canadas Stem Cell Network and a Senior Scientist at OHRI and Director of OHRIs Sprott Centre for Stem Cell ...

The interest in p27 expression in mammary carcinoma lies in its behaviour when examined in combination with other G1 cell cycle ... corresponding to their close interactions in regulating the G1/S transition in the cell cycle. Low levels of p27 were seen in ... Embryonic and induced Pluripotent Stem Cells * Adult Stem Cells * Haematopietic * Cancer Stem Cells ... The interest in p27 expression in mammary carcinoma lies in its behaviour when examined in combination with other G1 cell cycle ...

Cell Cycle. 2013; 12(17):2760-2773. https://doi.org/10.4161/cc.25951PubMedPubMed CentralGoogle Scholar ... Multiple Myeloma (MM) is an incurable plasma cell malignancy often treated by autologous stem cell transplant (ASCT). Clinical ... Figure 4.PARP1 and POLD2 co-expression highly prognostic in the setting of autologous stem cell transplant. For patients with ... Mice in our study did not receive stem cell transplants, which would better mirror the clinical setting of ASCT. Further study ...

Aging alters the cell cycle control and mitogenic signaling responses of human hematopoietic stem cells Colin A. Hammond, Colin ... A new mechanism for the aging of hematopoietic stem cells: aging changes the clonal composition of the stem cell compartment ... Platelet-biased stem cells reside at the apex of the haematopoietic stem-cell hierarchy ... Age-related changes of human bone marrow: a histometric estimation of proliferative cells, apoptotic cells, T cells, B cells ...

... in mouse embryonic stem cells. S. van der Laan, E. Golfetto, J. M Vanacker, and D. Maiorano (2014) ... Cell cycle-dependent expression of Dub3, Nanog and the p160 family of nuclear receptor coactivators (NCoAs) ... Cell cycle-dependent expression of Dub3, Nanog and the p160 family of nuclear receptor coactivators (NCoAs) in mouse embryonic ...

p38α Activates Purine Metabolism to Initiate Hematopoietic Stem/Progenitor Cell Cycling in Response to Stress. Cell Stem Cell. ... p38α Activates Purine Metabolism to Initiate Hematopoietic Stem/Progenitor Cell Cycling in Response to Stress. Cell Stem Cell, ... p38α Activates Purine Metabolism to Initiate Hematopoietic Stem/Progenitor Cell Cycling in Response to Stress, Cell Stem Cell ... p38α Activates Purine Metabolism to Initiate Hematopoietic Stem/Progenitor Cell Cycling in Response to Stress. In: Cell Stem ...

Scientists have discovered that a lack of stem cells in the womb lining is causing thousands of women to suffer from recurrent ... The lining has to renew itself each cycle, each miscarriage and successful birth. This renewal capacity is dependent on ... 20, 2020 Scientists find that normal intestinal cells de-differentiate en masse into stem cells that generate the cells ... Due to them, a lump of cells becomes an organ, and a fertilized egg develops into a baby. But what actually makes a stem cell? ...

This study traced intravenously administered induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (MSC) and ... Here, we recorded the menstrual cycle data in detail with uterine cavities observed and endometrial tissues detected after ... and hPSC-derived mesenchymal progenitor cells (also known as mesenchymal stem cells or MSCs) into adipogenic lineage cells. ... Animal Component-Free Cell Dissociation Kit Dissociation kit for human stem and progenitor cells ...