Integrating Multiple Microarray Data for Cancer Pathway Analysis Using Bootstrapping K-S Test
EWSR1-Smad4) in [31], all of them were detected based on large-scale protein-protein interaction experiments without annotation ... Nodes and edges represent human proteins and protein-protein interactions, respectively. Edges with direction represent a ... receptor-associated protein 1 is a Smad4 chaperone," The Journal of Biological Chemistry, vol. 276, no. 22, pp. 19495-19502, ... receptors can phosphorylate Smad2, Smad3, and Smad4 [28]. The SMAD family proteins then transduce antigrowth signals to the ...
Abnormal GRHL2 Methylation Confers Malignant Progression to Acute Leukemia
... signaling pathway-linked proteins, including TGF-,i,β,/i,, Smad2, Smad3, and Smad4. ,i,Conclusion,/i,. Increased ... Then we investigated the demethylating effect of arsenic trioxide and 5-azacitidine on the mRNA and protein expression levels ... and we found the elevated mRNA and protein levels of GRHL2 in acute leukemia cell lines after the use of the demethylation drug ... Secondly, we employed qRT-PCR and Western blotting to detect the mRNA and protein levels of GRHL2 in leukemia cell lines. Next ...
Combating Kidney Fibrosis
found that high glucose in mesangial cells induced sumoylation (by SUMO 2/3) of smad4, the co-smad essential for TGF-β-induced ... Universidad Autonoma Madrid, Spain) focused on gremlin, a well-known bone morphogenetic proteins (BMPs) antagonist. They found ...
Prediction of Differentially Expressed Genes and a Diagnostic Signature of Preeclampsia via Integrated Bioinformatics Analysis
Additionally, protein-protein interaction network analysis identified 10 hub genes, six (,i,FLT1,/i,, ,i,FLNB,/i,, ,i,FSTL3,/i ... Additionally, protein-protein interaction network analysis identified 10 hub genes, six (FLT1, FLNB, FSTL3, INHA, TREM1, and ... Several studies have demonstrated that Smad2, Smad4, and Smad7 may participate in PE via the EMT pathway [25, 26]. In this ... Protein-Protein Interaction (PPI) Network Analysis. For PPI network analysis, we used the search tool for the retrieval of ...
Integration of Gene Expression Profile Data to Verify Hub Genes of Patients with Stanford A Aortic Dissection
The protein-protein interaction networks of the DEGs were constructed according to the data from the STRING database. ... The protein-protein interaction networks of the DEGs were constructed according to the data from the STRING database. ... Downregulation of SMAD4 will also increase VSMCs proliferation and cause TAD pathological process [36]. Overexpression of HSP27 ... It contains seven WD40 repeats that are necessary for mediating protein-protein interactions [28]. Cdc20 plays an indispensable ...
MicroRNA-323a-3p Negatively Regulates NEK6 in Colon Adenocarcinoma Cells
... and protein quantification was performed using a BCA protein concentration determination kit (catalog number: P0012S; Beyotime ... β pathway by interfering with nuclear translocation Smad4 [24], and the activation of STAT3 signaling [25, 26]. ... A. M. Fry, L. ORegan, S. R. Sabir, and R. Bayliss, "Cell cycle regulation by the NEK family of protein kinases," Journal of ... c) Analysis of western blotting showed that NEK6 protein levels in COAD cell lines were higher than those in normal colon ...