Smad2 Protein / genetics * T-Box Domain Proteins / biosynthesis* * T-Box Domain Proteins / genetics ... specification by directly activating key lineage specification factors and indirectly by enhancing paracrine Nodal/Smad2 ...
Tsukazaki T, Chiang TA, Davison AF, Attisano L, Wrana JL (December 1998). "SARA, a FYVE domain protein that recruits Smad2 to ... Zinc finger FYVE domain-containing protein 9 or SARA (SMAD anchor for receptor activation) is a protein that in humans is ... It binds to the MH2 domain of the R-SMADs SMAD2 and SMAD3 as well as the type I TGF beta receptors. It facilitates the ... SARA contains a double zinc finger (FYVE domain). SARA is an anchoring protein involved in TGF beta signaling. ...
Its documented anti-SARS-CoV-2 activity has been attributed to its ability to inhibit spike-protein mediated and TGF-β- ... Its documented anti-SARS-CoV-2 activity has been attributed to its ability to inhibit spike-protein mediated and TGF-b- ... Platelets possess functional TGF-beta receptors and Smad2 protein. Platelets 18, 35-42. doi:10.1080/09537100600800743 ... Its documented anti-SARS-CoV-2 activity has been attributed to its ability to inhibit spike-protein mediated and TGF-β- ...
The EBNA1 protein interferes with tumor growth factor-beta signaling by downregulating Smad2; this interference with tumor- ... 28] In addition, the same protein may play a role in immune evasion via recruitment of regulatory T-helper cells. [29] The ... It is essential to know the structure of EBV and which proteins are expressed during different stages of its life cycle to ... Epstein-Barr virus vaccine development: a lytic and latent protein cocktail. Front Biosci. 2008 May 1. 13:5916-27. [QxMD ...
... fibroblasts 72 hours after recombinant adenoviral infection showed efficient deletion of Smad2 and Smad3 protein from Smad2/3fl ... alone or Smad2/3fl/fl alone) versus sham-operated mice (Supplemental Figure 4A). Deletion of Smad2, Smad3, or Smad2/3 from ... The data show that 70%-80% of the Adβ-gal-infected Smad2fl/fl, Smad3fl/fl, and Smad2/3fl/fl or Tgfbr1/2fl/fl fibroblasts became ... B) Quantification of selected mRNAs in Smad2/3-deleted EGFP+-activated fibroblasts from hearts of Smad2/3 PostnMCM/+ R26EGFP/+ ...
... urine protein (UP), and BUN in comparison to the control group. Protein and mRNA expressions of SMAD2, TGF-β1, connective ... There was also the downregulation of CTGF, FN, SMAD2, and TGF-β1 mRNA and protein expressions. Thus puerarin exerted its ... reduces phosphorylation of SMAD2/3 and impedes the transferring of SMAD2/3 from the pulp to the nucleus to combine with ... macrophage inflammatory proteins)-2, and MIP-1α levels in diabetic mice treated with whey protein. Additionally, levels of IL ( ...
Smad2, and Smad3 were measured by western blot. Cell apoptosis was analyzed by flow cytometry. Cytokine production was detected ... The protein levels of TGFBR2, Bcl-2, Bax, cleaved caspase 3, ... a and b) The protein levels of Smad2 and Smad3 were detected by ... Western blot indicated that overexpression of miR-128-3p reversed the LPS-induced promotion of Smad2 and Smad3 protein levels ... Next, protein samples were quantified using bicinchoninic acid (BCA) protein assay kit (Beyotime Biotechnology, Shanghai, China ...
Cell 12, 261-274 (2007). A requirement for intact microtubules and the motor protein kinesin-1 for efficient SMAD2 ... Alternatively spliced variant of SMAD2 lacking exon 3. Comparison with wild-type SMAD2 and SMAD3. J. Biol. Chem. 274, 703-709 ( ... Different SMAD2 partners bind a common hydrophobic pocket in SMAD2 via a defined proline-rich motif. EMBO J. 21, 145-156 (2002 ... The MAD-related protein SMAD7 associates with the TGFβ receptor and functions as an antagonist of TGFβ signaling. Cell 89, 1165 ...
... and Med15 interacts with Smad2/3 and Sterol regulatory element-binding protein. The current results show that, despite their ... In addition, the protein levels of CDK8 and CycC are up-regulated at the onset of the wandering stage, closely correlated with ... The adaptor protein Legless (Lgl)/Bcl-9 links Armβ-catenin to Pygopus (Pygo); the N-terminal homology domain (NHD) of Pygo is ... The protein, which migrates in SDS-PAGE with an apparent molecular mass of 28 kDa, sediments on native sucrose gradients with ...
... the effect of losartan on vascular remodeling and transforming growth factor-beta and phosphoinositide 3-kinase/protein kinase ... and TGF-β phosphorylates SMAD2/3 protein through activated TGFβR2 homodimer. It promotes the formation of SMAD2/3-SMAD4 complex ... The protein content of AKT in aorta of LG was reduced than that of MG, and the protein of p-Akt was raised than that of MG. ... The protein content of AKT in aorta of MG was raised than that of NG, and p-Akt protein was reduced than that of NG. The levels ...
Protein Processing, Post-Translational. MESH. Smad2 Protein/metabolism. MESH. T-Lymphocytes, Cytotoxic/pathology. MESH. ...
The Dyslexia-susceptibility Protein KIAA0319 Inhibits Axon Growth Through Smad2 Signaling. Cerebral cortex (New York, N.Y. : ... Leite, S. C., Sampaio, P., Sousa, V. F., Nogueira-Rodrigues, J., et al. (2016). The Actin-Binding Protein α-Adducin Is Required ...
Holtzhausen et al (2014) Novel bone morphogenetic protein signaling through Smad2 and Smad3 to regulate cancer progression and ... Zhou et al (2001) Role of AMP-activated protein kinase in mechanism of MetF. action. J.Clin.Invest. 108 1167 PMID: 11602624 Yu ... Kapeli et al (2016) Distinct and shared functions of ALS-associated proteins TDP-43, FUS and TAF15 revealed by multisystem ... Matsuda et al (2015) Comparative Study on the Cytoprotective Effects of Activated Protein C Treatment in Nonsteatotic and ...
Taken together, E proteins are novel Nodal signaling cofactors that associate with SMAD2/3 and FOXH1 and are necessary for ... We demonstrate that two bHLH proteins - HEB and E2A - bind the SCA motif at regions overlapping SMAD2/3 and FOXH1. Further, we ... This analysis has led to the elucidation of new proteins that mediate Nodal signaling; ones that play a key role in endoderm ... We have identified a new motif, termed SMAD Complex Associated (SCA) that is bound by SMAD2/3/4 and FOXH1 in human embryonic ...
Smad2/3 phosphorylation, and monocyte chemoattractant protein-1 in the lung in a mouse model, suggesting that H2S may be a ... COX: cyclooxygenases; NO: nitric oxide; MAPK: mitogen-activated protein kinase; ERK: extracellular regulated protein kinase; NF ... H. Ming-Ju, H. Yih-Shou, C. Tzy-Yen, and C. Hui-Ling, "Hepatitis C virus E2 protein induce reactive oxygen species (ROS)- ... S. Saha, P. K. Chakraborty, X. Xiong et al., "Cystathionine β-synthase regulates endothelial function via protein S- ...
Both SWCNT and MWCNT induced an overexpression of TGF-Beta1, TGF-Beta R1 and Smad2/3 proteins in lung fibroblasts compared with ... Chemical inhibition of ALK5 and short-hairpin (sh) RNA targeting of TGF-Beta R1 and Smad2 were used to probe the fibrogenic ... SWCNT- and MWCNT-induced collagen production was blocked by ALK5 inhibitor or shRNA knockdown of TGF-Beta R1 and Smad2. Our ... Proteins; Cellular-function; Humans; Cell-cultures; Immunological-tests; Chemical-inhibition; Ribonucleic-acids; Fibrogenesis; ...
The mRNA and protein levels of α smooth muscle actin (α-SMA) and Snail and the phosphorylation levels of Smad2/3, p38 mitogen- ... For example, ERK1/2 and stress activate protein kinase (SAPK)/JNK activation can inhibit or enhance Smad2 phosphorylation, ... proteins Smad2/3, which in turn form a complex with Smad4, thereby translocating to the nucleus and regulating target gene ... Protein concentrations were determined with the Micro BCA protein assay kit (Thermo Scientific, Waltham, MA) according to the ...
a) Representative figure of phospho-Smad2 protein and quantitation to actin protein by Western blot analysis in lung ... Phospho-Smad2 measurement. Phospho-Smad2 is a downstream signalling molecule of TGF-β1, and phosphorylation of Smad2 is ... 6a). Sivelestat treatment demonstrated a significant decrease of the phospho-Smad2 protein level induced by BLM. Sivelestat ... Phospho-Smad2 measurement. Western blot and densitometric analysis were performed as usual with phospho-Smad2 (Ser465/467) ( ...
Human Mothers Against Decapentaplegic Homolog 2 (Smad2) Protein. 20-abx068070 Abbexa * EUR 828.00 ... Human Smad2(Mothers Against Decapentaplegic Homolog 2) ELISA Kit. Human Mothers Against Decapentaplegic Homolog 2 (Smad2) ELISA ... for Mothers Against Decapentaplegic Homolog 2 (Smad2)ELISA kit. SEC124Mu-10x96wellstestplate Cloud-Clone 10x96-wells test plate ... Rat Mothers Against Decapentaplegic Homolog 2 (Smad2) ELISA Kit. SEC124Ra-1x48wellstestplate Cloud-Clone 1x48-wells test plate ...
It has been reported that TGFβ1 induces α-SMA production via phosphorylated Sma- and Mad-related protein 2/3 (Smad2/3); the ... The protein concentration was measured by the BCA protein assay kit. Equal amounts of protein (10 µg) were applied to each lane ... Western blot analysis revealed that phosphorylated Smad2/3 and α-SMA protein levels in CCD-18Co cells, which were increased by ... ab5694; Abcam), phosphorylated Smad2/3 (1:1,000; cat. no. 8828; Cell Signaling Technology, Inc.) and Smad2/3 (1:1,000; cat. no ...
... signaling pathway-linked proteins, including TGF-,i,β,/i,, Smad2, Smad3, and Smad4. ,i,Conclusion,/i,. Increased ... Then we investigated the demethylating effect of arsenic trioxide and 5-azacitidine on the mRNA and protein expression levels ... and we found the elevated mRNA and protein levels of GRHL2 in acute leukemia cell lines after the use of the demethylation drug ... Secondly, we employed qRT-PCR and Western blotting to detect the mRNA and protein levels of GRHL2 in leukemia cell lines. Next ...
This revealed an increase in phosphorylated SMAD2/SMAD3 protein levels, which was reduced by subsequent BMP7 treatment, whereas ... A, representative immunoblots probed for total SMAD2/SMAD3 (49 kDa) and phosphorylated SMAD2/SMAD3 (49 kDa) for TE7 cells ... A, representative immunoblots probed for total SMAD2/SMAD3 (49 kDa) and phosphorylated SMAD2/SMAD3 (49 kDa) for TE7 cells ... The transcriptional repressors are zinc finger proteins, including snail, SMAD-interacting protein 1 (SIP1; ref. 12), slug, and ...
Smad2 and Smad3 positively and negatively regulate TGF beta-dependent transcription through the forkhead DNA-binding protein ... Figure 1. E2a Is Required for Subsets of Smad2/3 Binding and for Global Patterns of Smad2/3 Target Gene Expression (A) Smad2/3 ... E protein silencing by the leukemogenic AML1-ETO fusion protein. 2004, Pubmed Zinzen, Combinatorial binding predicts spatio- ... smad2 snai1 stx6 tbxt tcf3 tsg101 vegt ventx1 wnt11 wnt5b zic3 znf703 Antibodies: GFP Ab5 Smad2 Ab9 Morpholinos: lefty MO7 tcf3 ...
B) SMAD1 expression at the protein level after no treatment (-) or treatment with 1 mM decitabine (+) for 96 h of two HL cell ... This hypothesis is further supported by observations in EBV-positive classic HL, in which decreased SMAD2 levels due to EBNA1- ... The impact of the promoter methylation status of SMAD1 on protein expression was further addressed by western blot analysis, ... Decitabine-treated samples of the DEV cell line are not shown because of lack of protein after demethylating treatment. (C) ...
Oncogene Proteins. Signal Transduction. Smad1 Protein. Smad2 Protein. Smad3 Protein. Transcriptional Regulator ERG. ... Smad1 Protein. Smad2 Protein. Smad3 Protein. End Stage Liver Disease. Epithelial-Mesenchymal Transition. Human Umbilical Vein ... Oncogene Proteins. Signal Transduction. Down-Regulation. Up-Regulation. Female. Male. ... identify a pathogenic mechanism where loss of ERG causes endothelial-dependent liver fibrogenesis via regulation of SMAD2/3. ...
Thus, loss of Smad3 in the context of one wild-type copy of Smad2 results in impaired production of anterior axial mesendoderm ... Finally, we demonstrate that Smad2;Smad3 double homozygous mutants entirely lack mesoderm and fail to gastrulate. Collectively ... while selective removal of both Smad2 and Smad3 from the epiblast additionally disrupts specification of axial and paraxial ... these results demonstrate that dose-dependent Smad2 and Smad3 signals cooperatively mediate cell fate decisions in the early ...
SMAD2 protein complex GO:0071142 * extrinsic component of omegasome membrane GO:0097629 ...
Smad2 Protein / metabolism; Smad3 Protein / metabolism; Time Factors; Transforming Growth Factor beta1 / metabolism ... Tumor Suppressor Protein p53 / genetics; Tumor Suppressor Protein p53 / physiology ...
SMAD2-SMAD4 protein complex GO:0071145 * mitochondrial inner membrane presequence translocase complex ...
Gene expression was determined by reverse transcription polymerase chain reaction (RT-PCR) and confirmed at the protein level ... Ski-related novel protein N (SnoN) and Smad ubiquitination regulatory factors (Smurfs)) and confirmed the expression of ... Bone morphogenic proteins (BMPs) play a key role in bone formation. Consequently, it was expected that topical application of ... regulated genes at the protein level. Smad7 and SnoN were significantly induced by rhTGFβ1 treatment while expression of Smad1 ...