Loading...
*  Abstract 12479: Delayed Tranilast Treatment Reduces Pathological Fibrosis Following Myocardial Infarction And In Uremic...
... profibrotic gene and phospho-Smad2 protein expression.. Results: Tranilast treatment in MI animals attenuated the change in ... These effects appear to be mediated via tranilast's direct actions on NCF via the Phospho-Smad2 pathway, and in part by its ... Phospho-Smad2 was reduced in NCF following tranilast treatment (by 65%, P,0.05). ...
http://circ.ahajournals.org/content/124/Suppl_21/A12479
*  Ablation of Smurf2 reveals an inhibition in TGF‐β signalling through multiple mono‐ubiquitination of Smad3 | The EMBO Journal
However, we did not detect any change in the protein levels of Smad2, Smad3, or the type I receptor in either Smurf1‐ or Smurf2 ... Loss of Smurf2 does not affect protein stability of Smad2/3 or TGF‐β‐induced phosphorylation. (A) Western blot analyses and (B ... Smurf2 does not regulate the protein stability of Smad2 or Smad3 in vivo. It was reported previously based on biochemical and ... In contrast, Smurf2 only caused slightly more ubiquitination of Smad2 following expression of Myc-Smurf2 and Flag-Smad2 in ...
http://emboj.embopress.org/content/30/23/4777
*  Smad2 and Myocardin-Related Transcription Factor B Cooperatively Regulate Vascular Smooth Muscle Differentiation From Neural...
TGF-β downstream signaling protein Smad2 is essential for VSMC differentiation from NCCs in vivo. Deletion of Smad2 in NCC ... Sagittal sections of wild-type (Smad2+/+, left) or Smad2 NCC-specific deleted embryonic day 11.5 mouse embryos (Smad2−/−, right ... C-E, MRTFB was required for Smad2-mediated VSMC marker mRNA and protein expression. Monc-1 cells were treated as in (A) and (B ... B, Smad2, but not Smad3, was essential for VSMC mark protein expression in NCCs. Monc-1 cells were treated similarly as ...
http://circres.ahajournals.org/content/113/8/e76
*  Human SMAD3 Antibody | Human SMAD2 Antibody
... elegans Sma proteins. SMAD2 and SMAD3 are intracellular signaling proteins and transcriptional regulators that are activated by ... These products are affinity-purified IgG antibodies that recognize different phosphorylated forms of human SMAD2/SMAD3 protein ... RNA Extraction and Protein Analysis for Real-Time qPCR * Direct cDNA Synthesis from ... TGF-beta type I receptor and cyclin-dependent kinases phosphorylate both SMAD2 and SMAD3 in C-terminal and linker regions. TGF- ...
http://www.clontech.com/US/Products/Cell_Biology_and_Epigenetics/Cancer_and_Inflammation/Smad3?sitex=10020:22372:US&PEBCL1=ZyGV5ASUEyim1V4kqlSbhHqcD5&PEBCL1_pses=ZGFA966EA6E6F95E0D39F28E378D3AD6A1B2926C74D032F0C1776C8A8C896BB52A1F998C6EFBFFD1ED58CA74061B55E8648D0FB7F336926CA2
*  Nuclear factor YY1 inhibits transforming growth factor beta- and bone morphogenetic protein-induced cell differentiation
YY1 interacts with the conserved N-terminal Mad homology 1 domain of Smad4 and to a lesser extent with Smad1, Smad2, and Smad3 ... Smad proteins transduce transforming growth factor beta (TGF-beta) and bone morphogenetic protein (BMP) signals that regulate ... Protein Binding, Protein Structure; Tertiary, RNA; Small Interfering/metabolism, Research Support; Non-U.S. Gov't, Reverse ... Nuclear factor YY1 inhibits transforming growth factor beta- and bone morphogenetic protein-induced cell differentiation. ...
http://uu.diva-portal.org/smash/record.jsf?pid=diva2:101622
*  CIN85 modulates TGF beta signaling by promoting the presentation of TGF beta receptors on the cell surface
We report here that T beta RI interacts with the SH3 domains of the adaptor protein CIN85 in response to TGF beta stimulation ... CIN85 enhanced TGF beta-stimulated Smad2 phosphorylation, transcriptional responses, and cell migration. CIN85 expression ...
http://www.diva-portal.org/smash/record.jsf?pid=diva2:848131
*  Anti-TGFBR1/Tgf Beta Receptor I Antibody
Total Protein Analysis. Electrophoresis/Gel Staining. Protein Transfer. Blocking Reagents. Chromogenic Development. ... Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is ... The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic ... Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. ...
https://www.bosterbio.com/anti-tgfbr1-antibody-pa1731.html
*  SMAD2 (phospho S467) & SMAD2 Protein Phosphorylation Antibody Pair | ABIN1340595
SMAD2 Protein Phosphorylation Antibody Pair o... , 产品编号 ABIN1340595. ... Find and order matched antibody pairs and products like this SMAD2 (phospho S467) & ... SMAD2 (phospho S467) & SMAD2 Protein Phosphorylation Antibody Pair SMAD2 (phospho S467) & SMAD2 Protein Phosphorylation ... This protein phosphorylation antibody pair set comes with two antibodies, one against the SMAD2 protein, and the other against ...
https://www.antibodies-online.cn/pair/1340595/SMAD2+phospho+S467++SMAD2+Protein+Phosphorylation+Antibody+Pair/
*  Smad2 - Mothers against decapentaplegic homolog 2 - Mus musculus (Mouse) - Smad2 gene & protein
Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 ... Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a ... Protein-protein interaction databases. BioGridi. 201275, 34 interactors. ComplexPortali. CPX-10 SMAD2-SMAD3-SMAD4 complex. CPX ... CPX-10 SMAD2-SMAD3-SMAD4 complex. CPX-13 SMAD2 homotrimer. CPX-3251 SMAD2-SMAD4 complex. ...
https://www.uniprot.org/uniprot/Q62432
*  SMAD2 - Mothers against decapentaplegic homolog 2 - Bos taurus (Bovine) - SMAD2 gene & protein
Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 ... Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a ... Protein-protein interaction databases. STRING: functional protein association networks. More...STRINGi. 9913.ENSBTAP00000017649 ... Protein-protein interaction databases. STRING: functional protein association networks. More...STRINGi. 9913.ENSBTAP00000017649 ...
http://www.uniprot.org/uniprot/Q1W668
*  Smad2/3 Proteins Are Required for Immobilization-induced Skeletal Muscle Atrophy. | Sigma-Aldrich
... is dispensable for denervation-induced muscle atrophy and Smad2/3 protein accumulation. Moreover, muscle-specific Smad2/3- ... Smad2/3 Proteins Are Required for Immobilization-induced Skeletal Muscle Atrophy.. [Toshimi Tando, Akiyoshi Hirayama, Mitsuru ... Here, we show that skeletal muscle immobilization elevates Smad2/3 protein but not mRNA levels in muscle, promoting atrophy. ... We also demonstrate that serum starvation promotes Smad2/3 protein accumulation in C2C12 myogenic cells, an in vitro muscle ...
https://www.sigmaaldrich.com/catalog/papers/27129272
*  The Dyslexia-susceptibility Protein KIAA0319 Inhibits Axon Growth Through Smad2 Signaling
AbstractKIAA0319 is a transmembrane protein associated with dyslexia with a presumed role in neuronal migration. Here we show ... At the mechanistic level, KIAA0319 engaged the JAK2-SH2B1 pathway to activate Smad2, which played a central role in KIAA0319- ... KIAA0319 is a transmembrane protein associated with dyslexia with a presumed role in neuronal migration. Here we show that ...
https://insights.ovid.com/cerebral-cortex/cecor/2017/03/000/dyslexia-susceptibility-protein-kiaa0319-inhibits/3/00008354
*  Tissue expression of SMAD2 - Staining in lymph node - The Human Protein Atlas
Expression of SMAD2 (JV18-1, MADH2, MADR2) in lymph node tissue. Antibody staining with HPA067203, CAB025507 and CAB073546 in ... Protein evidence (Ezkurdia et al 2014). Protein evidence (Kim et al 2014). RAS pathway related proteins. Ribosomal proteins. ... Plasma proteins. Potential drug targets. Predicted intracellular proteins. Predicted membrane proteins. Predicted secreted ... Protein expressioni. The protein expression bar, with the units not detected (n), low (l), medium (m) and high (h), is based on ...
http://www.proteinatlas.org/ENSG00000175387-SMAD2/tissue/lymph+node
*  Immunolocalisation and expression of Smad2 and Smad4 proteins in dog testis during postnatal development. - Semantic Scholar
Smad2 and Smad4 proteins, was investigated in immature and mature dog testis. Cellular localisation of Smad2 and Smad4 proteins ... the expression of Smad2 and Smad4 proteins in the Sertoli cells of mature testis was higher than in the immature group (P , ... The temporospatial distribution of Smad2 and Smad4 proteins in testicular cells suggests that these two signalling molecules ... Smad2 and Smad4 were widely expressed in the testes, mainly immunolocalised in the cytoplasm of gonocytes, Leydig cells and ...
https://www.semanticscholar.org/paper/Immunolocalisation-and-expression-of-Smad2-and-Sma-Zhang-He/15bc10b8285fb524dc94e898387a34ce0ff70a84
*  Targeted inhibition of activin receptor-like kinase 5 signaling attenuates cardiac dysfunction following myocardial infarction.
0/Smad2 Protein; 0/Transforming Growth Factor beta; EC 2.7.1.11/TGF-beta type I receptor; EC 2.7.11.1/Protein-Serine-Threonine ... Protein-Serine-Threonine Kinases / antagonists & inhibitors*. Pyrazoles / pharmacology*. Rats. Rats, Sprague-Dawley. Receptors ... Animals that received left anterior descending coronary artery ligation demonstrated systolic dysfunction, Smad2 activation, ... Smad2 (P , 0.01), alpha-smooth muscle actin (P , 0.001), and collagen I (P , 0.05) in the noninfarct zone of MI rats. ...
http://www.biomedsearch.com/nih/Targeted-inhibition-activin-receptor-like/20154262.html
*  Kinetics of Smad activation during PCMO culture.The act | Open-i
Kinetics of Smad activation during PCMO culture.The activation state of Smad2 (left) and Smad3 (right) in adherent (a) and ... for p-Smad2(Ser465/467) and p-Smad3(Ser423/425). Alpha-tubulin was used for normalization of p-Smad2 and p-Smad3 signal ... Protein-Serine-Threonine Kinases/metabolism. *Receptors, Transforming Growth Factor beta/metabolism. *Signal Transduction/ ... for p-Smad2(Ser465/467) and p-Smad3(Ser423/425). Alpha-tubulin was used for normalization of p-Smad2 and p-Smad3 signal ...
https://openi.nlm.nih.gov/detailedresult.php?img=PMC4338298_pone.0118097.g004&req=4
*  TGF-β directs Erk phosphorylation of Smad2 linker regi | Open-i
TGF-β directs Erk phosphorylation of Smad2 linker region.(A) Western blots from AKR-2B fibroblast cell lysates treated with TGF ... These proteins subsequently translocate into the nucleus to negatively or positively regulate gene expression. In this study, ... Blots were probed for smad2 phosphorylated within the linker region at S245, 250 and 255, striped and reprobed for total smad2 ... Blots were probed for smad2 phosphorylated within the linker region at S245, 250 and 255, striped and reprobed for total smad2 ...
https://openi.nlm.nih.gov/detailedresult.php?img=PMC3412844_pone.0042513.g004&req=4
*  TP53 polyclonal antibody - (PAB12719) - Products - Abnova
TP53 & MAPK1 Protein Protein Interaction Antibody Pair. *DI0251. * TP53 & SMAD2 Protein Protein Interaction Antibody Pair ... p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's ... This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell ... p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to ...
http://www.abnova.com/products/products_detail.asp?catalog_id=PAB12719
*  ZFIN Publication: Choi et al., 2007
Smad2 Protein/metabolism. *Transforming Growth Factor beta/metabolism. *Vascular Endothelial Growth Factor Receptor-2/genetics ... overexpression of FoxH1 has a negative effect on vascular formation that can be counteracted by the down-regulation of smad2 ...
http://zfin.org/ZDB-PUB-070303-21
*  Utility of Lymphoblastoid Cell Lines for Induced Pluripotent Stem Cell Generation
... β induced gene transcription through rapid degradation of SMAD2 protein; however, it does not affect the other SMAD proteins or ... β-responsive proteins, transcription of TGF-β and SMADs (particularly SMAD2, SMAD3, and SMAD4) was highly upregulated in LCLs, ... F. Wang, C. Gregory, C. Sample et al., "Epstein-Barr virus latent membrane protein (LMP1) and nuclear proteins 2 and 3C are ... SMAD2/3 pathway is also required for positive regulation of several factors of TGF-β signaling. These factors include NODAL, ...
https://www.hindawi.com/journals/sci/2016/2349261/
*  Frontiers | Accentuating and Opposing Factors Leading to Development of Thoracic Aortic Aneurysms Not Due to Genetic or...
SMAD2 protein and phosphorylated SMAD2 are increased in the medial layer of TAA of a degenerative cause (78). In contrast, ... SMAD-2. Although SMAD-2 is more well known for its presence in specific genetic causes of TAA, there are data that ... AKT2 (RAC-beta serine/threonine-protein kinase) or protein kinase B (PKB) and phospho-AKT levels are significantly reduced in ... protein levels of SMAD2, SMAD1/5/8, and phospho-SMAD1/5/8 were increased and SMAD4 was decreased from control values (80). A ...
https://www.frontiersin.org/articles/10.3389/fcvm.2015.00021/full
*  Heterozygous disruption of activin receptor-like kinase 1 is associated with increased arterial pressure in mice | Disease...
2007). Endoglin increases eNOS expression by modulating Smad2 protein levels and Smad2-dependent TGF-beta signaling. J. Cell ... The protein concentration was measured in the supernatants of each sample, and 1 µg of protein was mixed with the substrate ... To analyze the expression of ALK-1, the mRNA and protein levels were measured in lung and kidneys from Alk1+/− and Alk1+/+ ... urinary excretion of proteins (D; Alk1+/+, n=6; Alk1+/−, n=6) and urinary excretion of Na+ (E; Alk1+/+, n=6; Alk1+/−, n=6) in ...
http://dmm.biologists.org/content/8/11/1427?utm_source=DMM&utm_campaign=Nobel2017
*  Plus it
Western blot was probed for P-Smad2 protein. Image is representative of n = 6. C: representative Western blots of nuclear and ... P-Smad2 antibody was used to assess localization of "activated" Smad2. Arrows indicate infected cells. B: c-Ski forms a complex ... Ad-HA-Ski infection did not prevent P-Smad2 from accessing the nucleus as evidenced through strong nuclear staining of P-Smad2 ... and extracellular matrix protein expression (12). While the current study addresses c-Ski association with R-Smad2, our data ...
http://ajpcell.physiology.org/content/300/1/C176
*  Activin receptor inhibition by Smad2 regulates Drosophila wing disc patterning through BMP-response elements | Development
Expression of transgenic WT Smad2 protein with da-GAL4 in a Smad2 null restored normal disc shape (J versus I). Smad2-AAMA ... For rescue experiments, wild-type or mutated FLAG-tagged Smad2 proteins were expressed in a Smad2 mutant. Smad2-AAMA (alanine ... 2A). To determine whether the phenotype was caused by tissue-autonomous loss of Smad2 protein, we drove Smad2 RNAi with GAL4 ... As exemplified by loss of Baboon function caused by a mutant Smad2 protein, Smad proteins can cause loss of non-canonical ...
http://dev.biologists.org/content/140/3/649
*  Protocols and Video Articles Authored by David Warburton (Translated to Portuguese)
We also found increased Smad2 protein expression and phosphorylation, which was enhanced following cigarette smoke exposure, in ... Nov, 2009 , Pubmed ID: 19703997 Bcr and Abr are GTPase-activating proteins for the small GTPase Rac. Both proteins are ... Clara cell-specific protein, surfactant protein C, and aquaporin-5), and lack of surfactant secretion. Furthermore, canonical ... as documented by reduced PCNA-positive cell index and by reduced mRNA levels for surfactant protein C and Clara cell protein 10 ...
https://www.jove.com/author/David_Warburton?language=Portuguese