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*  Mung bean nuclease - Wikipedia
A single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions from the ends of DNA and RNA ... double-stranded RNA, or DNA / RNA hybrids. Mung Bean Nuclease catalyzes the specific degradation of single-stranded DNA or RNA ... The enzyme degrades single-stranded DNA or RNA to nucleoside 5'-monophosphates, but does not digest double-stranded DNA, ... Mung Bean Nuclease catalyzes the specific degradation of single-stranded DNA or RNA, and produces mono- and oligonucleotides ...
https://en.wikipedia.org/wiki/Mung_bean_nuclease
*  Who has used Mung Bean Nuclease and How Its effect?! - Molecular Biology
Mung Bean Nuclease is a single-stranded DNA and RNA endonuclease. It removes 3' and 5' extension from DNA or RNA, generating ... Mung bean is a single-strand-specific nuclease purified from germinated Mung bean sprouts. Degrades DNA and RNA to 5'- ... DNA and RNA, which are in double-stranded form, is resistant to this nuclease.. Hope it helps.. Cheers,. Haohao ... At near neutral pH, mung bean nuclease nicks supercoiled plasmid DNA in unwound regions. Cleavage is not strand-specific and ...
http://www.protocol-online.org/biology-forums/posts/20126.html
*  Strand-specific PCR of UV radiation-damaged genomic DNA revealed an essential role of DNA-PKcs in the transcription-coupled...
... first-strand synthesis of the single strand and the strand-specific PCR (Figure 3A) with a set of smart specific primers as ... Since RNA polymerase II is a substrate of DNA-PKcs [17], it was suggested that DNA-PK could act as a DNA damage-sensor that ... 2) Digestion of genomic DNA with T4 endonuclease V: As T4 endonuclease V (Endo V) has N-glycosylase and apurinic/apyrimidinic ... 2) The strand-specific PCR amplification of the transcribed strand or non-transcribed strand: For the strand-specific PCR, the ...
https://bmcbiochem.biomedcentral.com/articles/10.1186/1471-2091-12-2
*  Nucleodepolymerase | definition of Nucleodepolymerase by Medical dictionary
a single-strand-specific endonuclease that degrades DNA and RNA to nucleoside 5′-monophosphates. The enzyme is a single ... Widely used in molecular biology to map the position of mRNA to its DNA, and also used to remove single-stranded tails from DNA ... such as single- or double-stranded DNA or RNA.. nu·cle·ase (nū'klē-ās) General term for enzymes that catalyze the hydrolysis of ... For nucleases not listed here, see the specific term. Compare: exonuclease, endonuclease. ...
https://medical-dictionary.thefreedictionary.com/Nucleodepolymerase
*  Mung bean endonuclease - Wikipedia
Mung bean endonuclease is a single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions from ... the ends of DNA and RNA molecules, leaving blunt, ligatable ends. http://www.neb.com/nebecomm/products/productm0250.asp http:// ...
https://en.wikipedia.org/wiki/Mung_bean_endonuclease
*  OmniCleave™ Endonuclease :: DNA and RNA Nucleases :: Enzymes :: Lucigen
Lucigen strives to provide life scientists with the highest quality products and services for RNA/DNA amplification, cloning, ... Non-specific: Digests all types of DNA and RNA including single- or double-stranded linear, circular, and supercoiled ... and double-stranded DNA and RNA to di-, tri-, and tetranucleotides (Fig. 1). OmniCleave Endonuclease has the same substrate ... OmniCleave™ Endonuclease. Remove all types of RNA and DNA with this robust, flexible nuclease.. *. ...
http://www.lucigen.com/home.php?cat=277
*  Saccharomyces cerevisiae RNase H(35) Functions in RNA Primer Removal during Lagging-Strand DNA Synthesis, Most Efficiently in...
... cerevisiae possesses a single-stranded DNA-specific endonuclease activity that is able to act on double-stranded DNA in the ... RNases H are defined as ribonucleotide-specific endonucleases, cleaving the RNA portion of RNA-DNA/DNA or RNA/DNA duplexes. ... was used as a single-stranded RNA-DNA substrate (substrate 1) (RNA is in lowercase letters, and DNA is in capital letters). ... 45) indicates that the calf RNase HI is able to make a junction-specific cleavage on a single stranded RNA-DNA substrate. ...
https://mcb.asm.org/content/19/12/8361
*  Tagging to endogenous genes of Plasmodium falciparum using CRISPR/Cas9 | Parasites & Vectors | Full Text
Cas9 endonuclease is guided to a specific target DNA site by the single guide RNA (sgRNA) and subsequently induces double- ... In Plasmodium, the double-strand break repair by homologous recombination is largely favored since canonical NHEJ is deficient ... Programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012;337(6096):816-21.View ArticlePubMed ... Genomic DNA PCR to confirm the tagging. Using genomic DNA as the template and specific primers of each gene (P33/P34 for ck2β1 ...
https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-017-2539-0
*  ERCC5 Gene - GeneCards | ERCC5 Protein | ERCC5 Antibody
Endonuclease, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3incision in DNA nucleotide ... Inhibitory RNA Products. * Origene RNAi, sirna, and shrna products in human, mouse, rat for ERCC5 ... Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3incision in DNA nucleotide ...
http://www.genecards.org/cgi-bin/carddisp.pl?gene=ERCC5&rf=/home/genecards/current/website/carddisp.pl&go_func=9
*  Enzyme Explorer - Learning Center: Nucleases for DNA and RNA Digestion | China-Mainland | Sigma-Aldrich
RNA or both. Sigma offers a complete selection of high-purity nucleases to meet most digestion requirements. ... Purification of proteins and specific nucleic acids often requires the digestion of DNA, ... DNA. DNA. Hybrid. Non-Specific Nucleases. Endo-nuclease. Exo-nuclease. Single. Strand. Double Strand. RNA. RNA. Yields 3'- ... It degrades only the RNA component of the DNA-RNA hybrid (RNA that is hydrogen bonded to a complementary DNA strand).. ...
https://www.sigmaaldrich.com/china-mainland/zh/life-science/metabolomics/enzyme-explorer/learning-center/nucleases.html
*  OriGene - ERCC5 (NM 000123) cDNA Clone
Gene Summary: This gene encodes a single-strand specific DNA endonuclease that makes the 3' incision in DNA excision repair ... by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).. ... The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the ... This database is built by NCBI, and, provides only a single record for each gene/transcript. More details.. Due to SNPs, each ...
http://www.origene.com/human_cdna/NM_000123/SC322497.aspx
*  DNA Repair Protein Complementing XP-G Cells (ERCC5) Antikörper
This gene encodes a single-strand specific DNA endonuclease that makes the 3' incision in DNA excision repair following UV- ... The protein may also function in other cellular processes, including RNA polymerase II transcription, and transcription-coupled ... anti-DNA Repair Protein Complementing XP-G Cells Antikörper (ERCC5). Auf www.antikoerper-online.de finden Sie aktuell 91 DNA ... Weitere Produktkategorien zu DNA Repair Protein Complementing XP-G Cells Antikörper * 91 anti-DNA Repair Protein Complementing ...
http://www.antikoerper-online.de/dna-reparatur-pathway-28/ercc5-antibody-3260/
*  Single-Step qPCR and dPCR Detection of Diverse CRISPR-Cas9 Gene Editing Events in Vivo | G3: Genes | Genomes | Genetics
... directed by a single guide RNA (sgRNA) to the complementary DNA target site, to induce a site-specific double-strand break (DSB ... 2012 A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337: 816-821. ... a double-stranded linear DNA template was created by annealing a target-specific primer (TSP) with a common primer containing ... single guide RNA; ssODN, single-stranded oligodeoxynucleotide. ... Using this DNA sample collection, we validated specific ...
http://www.g3journal.org/content/7/10/3533
*  Method of electroporation-enhanced delivery of active agents - Patent # 6520950 - PatentGenius
... possessing the ability to specifically cleave other single-stranded RNA in a manner analogous to DNA restriction endonucleases ... Antisense nucleic acid sequences are DNA or RNA molecules that are complementary to at least a portion of a specific mRNA ... the DNA can be double stranded DNA, single-stranded DNA, complexed DNA, encapsulatedDNA, naked RNA, encapsulated RNA, and ... DNA immunization, a method to induce protective immune responses using "naked" DNA, complexed DNA or encapsulated DNA, is ...
http://www.patentgenius.com/patent/6520950.html
*  Nano-sensor array - The Board of Trustees of the Leland Stanford Junior University
Structure of the gene 2.5 protein, a single-stranded DNA binding protein encoded by bacteriophage T7. Proc Natl Acad Sci U S A ... Haurwitz, et al.,Sequence- and structure-specific RNA processing by a CRISPR endonuclease.,Science, Sep. 10, 2010,329(5997), ... Molecular structure of RNA polymerase and its complex with DNA. J Biochem. Jan. 1969;65(1):1-16. Kunin, et al.,Evolutionary ... Random array DNA analysis by hybridization. 2009-01-01. Drmanac. 20080318243. DNA measuring system and method. 2008-12-25. Haga ...
http://www.freepatentsonline.com/10059982.html
*  A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity | Science
... involving a dual-RNA structure that directs a Cas9 endonuclease to introduce site-specific double-stranded breaks in target DNA ... study further demonstrates that the Cas9 endonuclease family can be programmed with single RNA molecules to cleave specific DNA ... Our study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to ... Sequence- and structure-specific RNA processing by a CRISPR endonuclease. Science 329, 1355 (2010). doi:10.1126/science.1192272 ...
http://science.sciencemag.org/content/337/6096/816?ijkey=b1aeefa844a5d0e4a4718f6b6c41862be78de2f0&keytype2=tf_ipsecsha
*  Books - Lane Medical Library - Stanford University School of Medicine
Design and analysis of site-specific single-strand nicking endonucleases for gene correction / Michael J. Metzger and Michael T ... Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts ... [et al.] -- Generation of ... Sample preparation for small RNA massive parallel sequencing / Willemijn M. Gommans and Eugene Berezikov -- pt. II. Genomic ... sequence-specific modification of genomic DNA in eukaryotic cells by small DNA fragments/ Andrea Luchetti, Arianna Malgieri, ...
https://lane.stanford.edu/biomed-resources/eb.html?a=g
*  Advances in the delivery of RNA therapeutics: from concept to clinical reality | Genome Medicine | Full Text
... co-delivery of a single guide RNA (sgRNA) together with the mRNA encoding the Cas9 DNA endonuclease allows site-specific ... can be significantly improved by designing an asymmetric single-stranded DNA template that anneals to the non-target DNA strand ... Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell. 2015;163:759-71.PubMedPubMed CentralView Article ... therapeutic RNA delivery has been limited by a number of factors. Naked, single-stranded RNA is prone to nuclease degradation, ...
https://genomemedicine.biomedcentral.com/articles/10.1186/s13073-017-0450-0
*  DNA-free RNA Isolation
Particularly for downstream quantitative RT-PCR (qRT-PCR, co-amplified genomic DNA can lead to nonspecific results. ... the absence of genomic DNA is still a challenge. ... endonuclease which is able to digest single and double-stranded ... The enzyme is DNA specific without negative effect on the integrity of the remaining RNA (Vanecko and Lasbowski, 1961). ... 1.) Detection of genomic DNA in RNA samples. Different techniques have been used to monitor contamination of RNA by genomic DNA ...
https://lifescience.roche.com/en_pl/articles/dna-free-rna-isolation-rna-isolation.html
*  DNA-free RNA Isolation
The DNAse I enzyme is classified as an endonuclease which is able to digest single and double-stranded DNA into single bases or ... The enzyme is DNA specific without negative effect on the integrity of the remaining RNA (Vanecko and Lasbowski, 1961). ... 1.) Detection of genomic DNA in RNA samples. Different techniques have been used to monitor contamination of RNA by genomic DNA ... Very high levels of endogenous genomic DNA can still leave residual contaminating DNA in the isolated RNA. In case an ...
https://lifescience.roche.com/en_in/articles/dna-free-rna-isolation-rna-isolation.html
*  Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High...
The aftermath of the specific binding and action of the sgRNA-Cas9 complex on genomic DNA is the double-strand cleavage of DNA ... Kim, J. M., Kim, D., Kim, S., Kim, J. S. Genotyping with CRISPR-Cas-derived RNA-guided endonucleases. Nat. Commun. 5, 3157 ( ... In essence, the CRISPR/Cas9 system consists of two essential components-a single guide RNA (sgRNA), which recognizes and binds ... For the purpose of knocking out a gene, CRISPR/Cas9-mediated double-strand breaks recruit the non-homologous end-joining DNA ...
https://www.jove.com/video/55586/using-fluorescent-pcr-capillary-gel-electrophoresis-technique-to
*  Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system | PNAS
The mix of gRNA and Cas9 RNA was then injected into one-cell-stage embryos to induce RNA-guided targeted DNA double-stranded ... an endonuclease Cas9 and two small RNAs, CRISPR RNA (crRNA) and a transacting RNA (tracrRNA), are sufficient for RNA-guided ... A recent breakthrough using the purified Cas9 protein and designer gRNAs to achieve site-specific DNA double-stranded breaks in ... Recent studies show that a single guide RNA (gRNA) chimera that mimics the crRNA:tracrRNA complex can guide Cas9 to introduce ...
https://www.pnas.org/content/110/34/13904
*  Transfection of CRISPR/Cas9 Nuclease NLS ribonucleoprotein (RNP) into adherent mammalian cells using Lipofectamine® RNAiMAX
... and the Cas9 endonuclease. To create gene disruptions, a single guide RNA is generated to direct the ... ... The CRISPR/Cas genome editing system consists of a single guide RNA (sgRNA) ... a single guide RNA is generated to direct the Cas9 nuclease to a specific genomic location. Cas9-induced double strand breaks ... are repaired via the Non Homologous End Joining (NHEJ) DNA repair pathway. The repair is error prone, and thus insertions and ...
https://www.diagenode.com/en/protocols/cas9-nls-protocol
*  Interactive Fly, Drosophila
Unlike endonucleases that recognize a specific DNA sequence, FEN-1 recognizes a specific DNA structure, independent of the DNA ... single-stranded DNA (5' flap). This 'double-flap' or 'overlap-flap' structure results from DNA polymerase activity that ... accessory protein PCNA removes RNA primers or damaged DNA, generating a product for ligation by DNA ligase I. Several lines of ... PCNA binds to flap endonuclease 1 (FEN-1), a structure-specific endonuclease involved in DNA replication. There is a direct ...
http://www.sdbonline.org/sites/fly/polycomb/pcna2a.htm