Promoters for Chlamydia Type III Secretion Genes Show a Differential Response to DNA Supercoiling That Correlates with Temporal...
Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis. Proc. Natl. Acad. Sci. U. S. A ... The topoisomers were resolved on 1.4% agarose gels in 1× TAE buffer (40 mM Tris-acetate, 1 mM EDTA) with concentrations of ... in a total of 40 μl containing 50 mM Tris-HCl (pH 7.6), 0.1 mM EDTA, 1 mM dithiothreitol, 50 mM NaCl, 10% glycerol, and various ...