Loading...
*  Site-specific recombinase technology - Wikipedia
In the following "knock-in" step, the tagged genomic sequence was replaced by homologous genomic sequences with certain ... These efforts mark the top of various "tag-and-exchange" strategies, which are dedicated to tagging a distinct genomic site ... Turan, S.; Bode, J. (2011). "Site-specific recombinases: From tag-and-target- to tag-and-exchange-based genomic modifications ... The tagging step per se may address certain classes of integration sites by exploiting integration preferences of retroviruses ...
https://en.wikipedia.org/wiki/Site-specific_recombinase_technology
*  TRAPPC2 - Wikipedia
1993). "Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library". Nat. ... 2004). "Identification of three novel SEDL mutations, including mutation in the rare, non-canonical splice site of exon 4". ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ... coding sequence are not a common cause of early primary osteoarthritis in men". Clin. Genet. 62 (1): 94-5. doi:10.1034/j.1399- ...
https://en.wikipedia.org/wiki/TRAPPC2
*  BDH1 - Wikipedia
1997). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807-28. doi:10.1101/gr.6.9.807. ... site-directed mutagenesis of a new recombinant fusion protein". Biochemistry. 39 (32): 9687-97. doi:10.1021/bi000274z. PMID ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...
https://en.wikipedia.org/wiki/BDH1
*  Cancer Genome Anatomy Project - Wikipedia
... and physically mapped using sequence-tagged sites (STS). The data for BAC clones are also available through CGAP and NCBI ... The resultant part of the sequence was termed the expressed sequence tag (EST) and is always at the end of the sequence close ... This allows for easier identification of a SAGE tag's corresponding sequence. In addition, there are tools associated with ... The sequencing of cDNA will produce the entire mRNA transcript that generated it. Practically, only part of the sequence is ...
https://en.wikipedia.org/wiki/Cancer_Genome_Anatomy_Project
*  Cis-natural antisense transcript - Wikipedia
Another resource is the extensive expressed sequence tag (EST) libraries but these small sequences must first be assigned an ... Currently there are a variety of web and software resources that can be used to look for antisense pairs. The NATsdb or Natural ... Studies that use mRNA have sequences whose orientations are known, but the amount of mRNA sequence information available is ... In the areas of overlap between the cis-NATs the RNA polymerases will collide and stop at the crash site. Transcription is ...
https://en.wikipedia.org/wiki/Cis-natural_antisense_transcript
*  Transcriptomics technologies - Wikipedia
... low-throughput sequencing using the Sanger method was used to sequence random transcripts, producing Expressed Sequence Tags ( ... informed by canonical splice site sequences and known intron splice site information. Identification of intron splice junctions ... An expressed sequence tag (EST) is a short nucleotide sequence generated from a single RNA transcript. RNA is first copied as ... Unique molecular identifiers (UMIs) are short random sequences that are used to individually tag sequence fragments during ...
https://en.wikipedia.org/wiki/Transcriptomics_technologies
*  ATP6V1G1 - Wikipedia
"Identification of genes expressed in human CD34+ hematopoietic stem/progenitor cells by expressed sequence tags and efficient ... The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of three V1 domain G subunit proteins. ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...
https://en.wikipedia.org/wiki/ATP6V1G1
*  Optical mapping - Wikipedia
... assembled sequence contigs can be scanned for restriction sites in silico using known sequence data and aligning them to the ... Other categories of methods include using microarrays, pulsed-field gel electrophoresis, cytogenetics and paired-end tags. ... Optical sequencing is a single molecule DNA sequencing technique that follows sequence-by-synthesis and uses optical mapping ... While most next generation sequencing technologies aim of massive amounts of smalls sequence reads, these small sequence reads ...
https://en.wikipedia.org/wiki/Optical_mapping
*  Restriction site associated DNA markers - Wikipedia
The most efficient way to find RAD tags is by high-throughput DNA sequencing, called RAD tag sequencing, RAD sequencing, RAD- ... The use of the flanking DNA sequences around each restriction site is an important aspect of RAD tags. The density of RAD tags ... disrupt restriction sites and lead to the absence of RAD tags or substantial DNA sequence polymorphisms that disrupt RAD tag ... The use of high-throughput sequencing to analyze RAD tags can be classified as reduced-representation sequencing, which ...
https://en.wikipedia.org/wiki/Restriction_site_associated_DNA_markers
*  Peptide microarray - Wikipedia
... pathways Detection of contaminating enzyme activities Consensus sequence and key residues determination Identifying sites for ... After scanning the microarray slides, the scanner records a 20-bit, 16-bit or 8-bit numeric image in tagged image file format ... epitope spreading Flexible design for peptide sequence (i.e. posttranslational modifications, sequence diversity, non-natural ... Usually, the secondary antibody is tagged by a fluorescence label that can be detected by a fluorescence scanner. Other label- ...
https://en.wikipedia.org/wiki/Peptide_microarray
*  Diazirine - Wikipedia
The carbene compound may include a bioorthogonal tag or handle by which the protein of interest can be isolated. The protein ... The carbene will covalently bond to residues in the binding site of the receptor. ... can then be digested and sequenced by mass spectrometry in order to the identity which residues the carbene containing ligand ... Researchers used a plant hormone analog with a diazirine crosslinking moiety and a biotin tag for isolation to identity the new ...
https://en.wikipedia.org/wiki/Diazirine
*  DERB - Wikipedia
Reporter tags for FRET, BiFC, PFC etc. detection. Recombinase recognizable sites for the insertion of ORF of the proteins of ... The sequences of two or more separate sets of promoters. ... which produces two or more proteins which are labeled or tagged ...
https://en.wikipedia.org/wiki/DERB
*  Vector (molecular biology) - Wikipedia
... but a protease cleavage site positioned in the polypeptide linker region between the protein and the tag allows the tag to be ... Translation initiation site - Shine-Dalgarno sequence enclosed in the RBS, 8 base-pairs upstream of the AUG start codon. ... Protein purification tags: Some expression vectors include proteins or peptide sequences that allows for easier purification of ... Cloning site: This may be a multiple cloning site or other features that allow for the insertion of foreign DNA into the vector ...
https://en.wikipedia.org/wiki/Vector_(molecular_biology)
*  Epistasis and functional genomics - Wikipedia
Tagging at the deletion sites with molecular barcodes, unique 20-bp sequences, allows for the identification and study of ... Sequencing of the entire yeast genome has made it possible to generate a library of knock-out mutants for nearly every gene in ...
https://en.wikipedia.org/wiki/Epistasis_and_functional_genomics
*  Cas9 - Wikipedia
This approach is made possible by hybridizing ssDNA with a PAM complement sequence to ssRNA allowing for a dsDNA-RNA PAM site ... ability to isolate endogenous RNA transcripts in cells without the need to induce chemical modifications to RNA or RNA tagging ... Although the low sequence similarity, the sequence similar to RNase H has a RuvC fold (one member of RNase H family) and the ... Previous sequence analysis and biochemical studies have suggested Cas9 contain RNase H and HNH endonuclease homologous domains ...
https://en.wikipedia.org/wiki/Cas9
*  Immunomics - Wikipedia
The first set of clones were selected if identified expressed sequence tags (ESTs) were unique or enriched specifically in ... Epitope mapping identifies the sites of antibodies to which their target antigens bind. In the past, scientists would have to ... Only specific peptide sequences from some pathogen-specific antigens are needed to stimulate T- and B- cell responses; that is ... The 'immunome' of a pathogen is described by its set of epitopes, and can be defined by comparing genome sequences and applying ...
https://en.wikipedia.org/wiki/Immunomics
*  GATA4 - Wikipedia
2000). "A minimal critical region of the 8p22-23 amplicon in esophageal adenocarcinomas defined using sequence tagged site- ... Evans T, Reitman M, Felsenfeld G (1988). "An erythrocyte-specific DNA-binding factor recognizes a regulatory sequence common to ...
https://en.wikipedia.org/wiki/GATA4
*  Factor X - Wikipedia
... after the arginine in the sequence Ile-Glu/Asp-Gly-Arg, IEGR or IDGR) can easily be engineered between a tag sequence and the ... The active site of factor Xa is divided into four sub pockets as S1, S2, S3 and S4. The S1 subpocket determines the major ... The S4 sub-pocket has three ligand binding domains: the "hydrophobic box", the "cationic hole" and the water site. Factor Xa ... McMullen BA, Fujikawa K, Kisiel W, Sasagawa T, Howald WN, Kwa EY, Weinstein B (June 1983). "Complete amino acid sequence of the ...
https://en.wikipedia.org/wiki/Factor_X
*  Epigenetics of physical exercise - Wikipedia
... the same region most transcription factors use to read a DNA sequence. A common epigenetic tag found in DNA is the covalent ... CpG sites in a gene are most commonly found in the promoter regions of a gene while also being present in non promoter regions ... Telomeres are repetitive sequences located at the end of chromosomes whose purpose are to slow the process of shortening and ... The CpG sites in non promoter regions tend to be constitutively methylated, causing transcription machinery to ignore them as ...
https://en.wikipedia.org/wiki/Epigenetics_of_physical_exercise
*  Restriction fragment length polymorphism - Wikipedia
"Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia". ... TRFLP works by PCR amplification of DNA using primer pairs that have been labeled with fluorescent tags. The PCR products are ... The sequence changes directly involved with an RFLP can also be analyzed more quickly by PCR. Amplification can be directed ... In allele "a", restriction site 2 has been lost by a mutation, so the probe now detects the larger fused fragment running from ...
https://en.wikipedia.org/wiki/Restriction_fragment_length_polymorphism
*  TANGO2 - Wikipedia
Expression data from Expressed Sequence Tag mapping, microarray and in situ hybridization show high expression for Homo sapiens ... 1] www.tango2.it - Disease website [2] www.tango2research.org - Research disease website -. ... It is characterized by the NRDE superfamily domain (DUF883), which is strictly known for the conserved amino acid sequence of ( ... sites of neuromuscular junctions, and in neurons. The NRDE (DUF883) domain, is a domain of unknown function spanning majority ...
https://en.wikipedia.org/wiki/TANGO2
*  CCNB1IP1 - Wikipedia
1997). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807-28. doi:10.1101/gr.6.9.807. ... Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788-95 ... 2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40-5. doi:10.1038/ ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...
https://en.wikipedia.org/wiki/CCNB1IP1
*  Tracking - Wikipedia
... finding the location in each frame of a video sequence Web visitor tracking, the analysis of visitor behavior on a website ... Animal migration tracking, performed by attaching a tag to an animal Environmental tracking, a concept developed by the ...
https://en.wikipedia.org/wiki/Tracking
*  Outline of genetics - Wikipedia
... ribosome risk communication sequence-tagged site (STS) sex chromosome sex-linked shotgun sequencing single-nucleotide ... mutation non-coding DNA non-directiveness nonsense mutation Northern blot Nucleic acid sequence nucleus oligo oncogene ... contig craniosynostosis cystic fibrosis cytogenetic map deletion diabetes mellitus diploid DNA replication DNA sequencing ... screening genome genotype germ line haploid haploinsufficiency hematopoietic stem cell heterozygous highly conserved sequence ...
https://en.wikipedia.org/wiki/Outline_of_genetics
*  Template switching polymerase chain reaction - Wikipedia
... where the polyadenylation site binds to a bead-specific primer containing a unique identifying sequence. This primer also ... subsequent rounds of PCR will incorporate the tag, which permits each isolated cDNA that is sequenced to be tracked back to a ... amplification that relies on a natural PCR primer sequence at the polyadenylation site and adds a second primer through the ... of contaminating rRNA sequence. Frequently the goal is some form of whole transcriptome shotgun sequencing. It is marketed by ...
https://en.wikipedia.org/wiki/Template_switching_polymerase_chain_reaction