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*  MS2 tagging - Wikipedia
When tracking more than one RNA sequence in the nucleus of cultured cells, more than one target sequence is needed. This could ... More recently, the technique has been used to monitor the appearance of RNA in living cells, at the site of transcription, or ... Both the RNA that tagged with MS2 and the MS2 protein tag were expressed, and then, the affinity interaction was used to help ... The MS2 tag is also effective for small molecules when an MS2 coat protein is used to isolate a variety of ribonucleoprotein ...
https://en.wikipedia.org/wiki/MS2_tagging
*  SAG (gene) - Wikipedia
2002). "Expressed sequence tag analysis of human retina for the NEIBank Project: retbindin, an abundant, novel retinal cDNA and ... Lee KB, Ptasienski JA, Bunemann M, Hosey MM (2000). "Acidic amino acids flanking phosphorylation sites in the M2 muscarinic ... Yamaki K, Tsuda M, Shinohara T (1988). "The sequence of human retinal S-antigen reveals similarities with alpha-transducin". ... "Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence" (PDF). ...
https://en.wikipedia.org/wiki/SAG_(gene)
*  Re2c - Wikipedia
... stag an s-tag: saves the last input position at which @stag matches in a variable named stag #mtag an m-tag: saves all input ... Each block consists of a sequence of rules, definitions and configurations (they can be intermixed, but it is generally better ... See the official re2c website for more complex examples. #include static int lex(const char *YYCURSOR) { const char *YYMARKER ... Ulya, Trofimovich (2017). "Tagged Deterministic Finite Automata with Lookahead" (PDF). draft. Ville, Laurikari (2001). " ...
https://en.wikipedia.org/wiki/Re2c
*  DNA footprinting - Wikipedia
Binding sites of transcription factors have been successfully identified this way. Next-generation sequencing has enabled a ... sequencing gels or capillary electrophoresis have been successful in analyzing footprinting of fluorescent tagged fragments. A ... Site-centric methods, on the other hand, find footprints given the open chromatin profile around motif-predicted binding sites ... Thus is more likely to have its action blocked by a bound protein on a DNA sequence. In addition, the DNase I enzyme is easily ...
https://en.wikipedia.org/wiki/DNA_footprinting
*  ZNF44 - Wikipedia
2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags". Proc. Natl. Acad. Sci. U.S.A. 97 (7): ... "Clustering of C2-H2 zinc finger motif sequences within telomeric and fragile site regions of human chromosomes". Genomics. 13 ( ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. doi:10.1016/ ...
https://en.wikipedia.org/wiki/ZNF44
*  Mark Boguski - Wikipedia
One database effort in particular, the database of Expressed Sequence Tags (dbEST, 1993), has enjoyed a particularly long and ... this was the first instance of Science magazine using the World Wide Web to publish results, provide hyper-linked information ... Division of Biology and Biomedical Sciences Medical Scientist Training Program Bioinformatics Expressed Sequence Tags database ... Sequence analysis of the NF1 gene and its GTPase-activating (GAP) domain led Boguski and his team into the ras pathway where ...
https://en.wikipedia.org/wiki/Mark_Boguski
*  Ceramide kinase - Wikipedia
Sequence analysis of CERK putatively suggests that the following post-translational modification sites exist: 4 N-glycosylation ... BLAST searches of expressed sequence tag (ESTs) by Sugiura and colleagues have yielded results showing orthologous CERK genes ... sites, 15 phosphorylation sites, 5 prenylation sites, and 2 amidation sites. The complete gene of mouse CERK differed slightly ... The decreased length of the open reading frame results in the loss of 2 prenylation sites and 1 amidation site. In human CERK, ...
https://en.wikipedia.org/wiki/Ceramide_kinase
*  List of birds of American Samoa - Wikipedia
The following tags have been used to highlight several categories, but not all species fall into one of these categories. Those ... Hunting and introduced predators have reduced their numbers but there are still some important breeding sites such as Lata ... This list's taxonomic treatment (designation and sequence of orders, families and species) and nomenclature (common and ...
https://en.wikipedia.org/wiki/List_of_birds_of_American_Samoa
*  Strep-tag - Wikipedia
Over the years, the Strep-tag was systemically optimized, to permit a greater flexibility in the choice of attachment site. ... This peptide sequence exhibits intrinsic affinity towards Strep-Tactin®, a specifically engineered streptavidin and can be N- ... Just like other short-affinity tags (His-tag, FLAG-tag), the Strep-tag can be easily fused to recombinant proteins during ... Streptamer Protein tag Schmidt, Thomas GM; Skerra, Arne (2007). "The Strep-tag system for one-step purification and high- ...
https://en.wikipedia.org/wiki/Strep-tag
*  Fluorescence in the life sciences - Wikipedia
Automated sequencing of DNA by the chain termination method; each of four different chain terminating bases has its own ... The DNA microarray Immunology: An antibody has a fluorescent chemical group attached, and the sites (e.g., on a microscopic ... many biological molecules have an intrinsic fluorescence that can sometimes be used without the need to attach a chemical tag. ... Bilirubin, for instance, is highly fluorescent when bound to a specific site on serum albumin. Zinc protoporphyrin, formed in ...
https://en.wikipedia.org/wiki/Fluorescence_in_the_life_sciences
*  TEV protease - Wikipedia
The preferred, native cleavage sequence was first identified by examining the cut sites in the native polyprotein substrate for ... One of the main uses of this protein is for removing affinity tags from purified recombinant fusion proteins. The reason for ... 7 cleavage sites). The native TEV protease also contains an internal self-cleavage site. This site is slowly cleaved to ... Carrington JC, Dougherty WG (May 1988). "A viral cleavage site cassette: identification of amino acid sequences required for ...
https://en.wikipedia.org/wiki/TEV_protease
*  Hardlink - Wikipedia
SMS tags and RFID tags. The hardlink method establishes a reference link between a physical world object and a .mobi web page ... and enters some alphanumeric sequence found on the target object. This alphanumeric sequence may be the objects part number or ... The hardlink method does not require a graphical object tag or any special software be loaded on the users cell phone, but does ... Unlike Japan, few US cell phone providers currently offer graphical tag readers or other support for object hyperlinking ...
https://en.wikipedia.org/wiki/Hardlink
*  Chemical biology - Wikipedia
iTRAQ, Tandem mass tags (TMT) and Isotope-coded affinity tag (ICAT) are protein mass-tags that consist of a covalently ... Samples for Proteomics contain a myriad of peptide sequences, the sequence of interest may be highly represented or of low ... Development of new enrichment strategies is needed in areas like non-ser/thr/tyr phosphorylation sites and other post ... "Oligopeptide biases in protein sequences and their use in predicting protein coding regions in nucleotide sequences". Proteins ...
https://en.wikipedia.org/wiki/Chemical_biology
*  PBDC1 - Wikipedia
There are also multiple conserved phosphorylation sites located in the amino acid sequence at tyrosine 72 and serine 126. ... The BABAM1 interaction was found in both databases using an anti-tag coimmunoprecipitation assay while POLR2H was based on a ... A multiple sequence alignment of 20 orthologous protein sequences reveals very strong conservation of the polysaccharide ... There were no other predicted exons within the genomic CXorf26 sequence when 3000 base pairs were added on either side in the ...
https://en.wikipedia.org/wiki/PBDC1
*  Repressilator - Wikipedia
... a carboxy terminal tag based on the ssRA RNA sequence was added at the 3' end of each repressor gene. This tag is recognized by ... It was found that these oscillations were favoured by strong promoters coupled to efficient ribosome binding sites, tight ... regions were replaced with a tighter hybrid promoter which combined the λ PL promoter with LacL and TetR operator sequences. ...
https://en.wikipedia.org/wiki/Repressilator
*  XDNA - Wikipedia
... and can be used as an affinity tag for interstrand bonding. DNA RNA DNA sequencing Genetic engineering Nanobiotechnology ... yDNA also uses a benzene ring, similar to xDNA, with the only difference being the site of addition of the aromatic ring. The ... and its high-affinity binding to complementary DNA and RNA sequences means that it can not only help locate these sequences ... In the end, which of the two has the strongest overall stacking interactions is dependent on the sequence; xT and yT bind A ...
https://en.wikipedia.org/wiki/XDNA
*  Autotaxin - Wikipedia
"Shotgun sequencing of the human transcriptome with ORF expressed sequence tags". Proceedings of the National Academy of ... Koh E, Clair T, Woodhouse EC, Schiffmann E, Liotta L, Stracke M (May 2003). "Site-directed mutations in the tumor-associated ... and partial sequence analysis of autotaxin, a novel motility-stimulating protein". The Journal of Biological Chemistry. 267 (4 ... "Stimulation of tumor cell motility linked to phosphodiesterase catalytic site of autotaxin". The Journal of Biological ...
https://en.wikipedia.org/wiki/Autotaxin
*  Protein methods - Wikipedia
Fusion proteins are made by inserting protein tags, such as the His-tag, to produce a modified protein that is easier to track ... Some proteins have never been directly sequenced, however by translating codons from known mRNA sequences into amino acids by a ... See genetic code.) Site-directed mutagenesis selectively introduces mutations that change the structure of a protein. The ... Some examples of genetic methods include conceptual translation, Site-directed mutagenesis, using a fusion protein, and ...
https://en.wikipedia.org/wiki/Protein_methods
*  Protein purification - Wikipedia
This often involves engineering a protease cleavage site between the tag and the protein. High performance liquid ... A common technique involves engineering a sequence of 6 to 8 histidines into the N- or C-terminal of the protein. The ... Recombinant expression allows the protein to be tagged, e.g. by a His-tag or Strep-tag, to facilitate purification, which means ... Another way to tag proteins is to engineer an antigen peptide tag onto the protein, and then purify the protein on a column or ...
https://en.wikipedia.org/wiki/Protein_purification
*  Web analytics - Wikipedia
... page tagging or 'Web bugs'. In the mid-1990s, Web counters were commonly seen - these were images included in a web page that ... Sometimes it is used interchangeable with the term "loyalty." Click path - the chronological sequence of page views within a ... There are at least two categories of web analytics; off-site and on-site web analytics. Off-site web analytics refers to web ... visitor Web bug Website correlation Website localization Web log analysis software Web mining Web traffic Web traffic ...
https://en.wikipedia.org/wiki/Web_analytics
*  CLIP - Wikipedia
Tag-Based Next Generation Sequencing. p. 153. doi:10.1002/9783527644582.ch10. ISBN 978-3527644582. König, J; Zarnack, K; ... CLIP-seq depends on cross-linking induced mutation sites (CIMS) to localized protein-RNA binding sites. Because CIMS are ... The isolated RNA is converted into a cDNA library and deep sequenced using high-throughput sequencing technology. Cross-linking ... Because reverse transcription stops prematurely at the cross-link site, iCLIP allows RNA-protein interaction sites to be ...
https://en.wikipedia.org/wiki/CLIP
*  LGR5 - Wikipedia
Sequence alignment showed that the second N-glycosylation site in LGR5 (Asn 208) aligns with that on the sixth repeat of ... California using expression sequence tags based on putative glycoprotein hormone receptors in Drosophila. Experimental evidence ... Sequence analyses showed that the transmembrane regions and cysteine-flanked junction between TM1 and the extracellular domain ...
https://en.wikipedia.org/wiki/LGR5
*  Init - Wikipedia
To remove the requirement to edit /etc/rc, BSD variants have long supported a site-specific /etc/rc.local file that is run in a ... The order in which scripts are executed is determined by the rcorder script based on the requirements stated in these tags. ... sub-shell near the end of the boot sequence. A fully modular system was introduced with NetBSD 1.5 and ported to FreeBSD 5.0 ... this system uses explicit dependency tags placed within each script. ...
https://en.wikipedia.org/wiki/Init
*  SORBS2 - Wikipedia
"Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... ArgBP2 contains several potential Abl phosphorylation sites; Arg and c-Abl represent the mammalian members of the Abelson ... Hartley JL, Temple GF, Brasch MA (Nov 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 (11 ... sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research. 11 (3): 422-35. doi:10.1101/gr. ...
https://en.wikipedia.org/wiki/SORBS2
*  PARM1 - Wikipedia
2002). "Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, ... Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788-95 ... 2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40-5. doi:10.1038/ ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...
https://en.wikipedia.org/wiki/PARM1