"Linkage disequilibrium across six prion gene regions spanning 20 kbp " by Benedict T. Green, Michael P. Heaton et al.
Four sequence tagged sites (i.e., STS or amplicons) totaling 3869 bp and spanning 20 kbp of genomic PRNP sequence were ... Analysis of these sequences identified 36 previously unreported polymorphisms. In combination with two previously characterized ... sequenced in a diverse panel of 90 sires representing ten popular U.S. breeds of sheep. ... coding sequence of domestic sheep (Ovis aries) are associated with genetic predisposition to scrapie, a transmissible ...http://digitalcommons.unl.edu/hruskareports/320/
"Expressed sequence tags from <i>Diabrotica virgifera virgifera</i> mid" by Blair D. Siegfried, N....
Of the remaining sequences, most predict proteins with either catalytic (62%) or binding (19%) functions, as expected for ... Here we describe a collection of 691 sequences from the Western corn rootworm midgut, 27% of which predict proteins with no ... Expressed sequence tags from Diabrotica virgifera virgifera midgut identify a coleopteran cadherin and a diversity of ... Of the remaining sequences, most predict proteins with either catalytic (62%) or binding (19%) functions, as expected for ...https://digitalcommons.unl.edu/entomologyfacpub/74/
"Adenovirus Preterminal Protein Binds to the CAD Enzyme at Active Sites" by Peter C. Angeletti and Jeffrey A. Engler
Amino acid sequencing of the 150-kDa band revealed sequence identity to carbamyl phosphate synthetase I (CPS I) and a high ... Additionally, in vitro-translated epitope-tagged pTP and CAD were immunoprecipitable as a complex, indicating the occurrence of ... Both pTP and CAD were confirmed to colocalize with active sites of replication detected by bromodeoxyuridine incorporation. ... replicative complexes form at discrete sites on the nuclear matrix (NM) via an interaction mediated by the precursor of the ...http://digitalcommons.unl.edu/virologypub/29/
"Effect of Diet on the Rumen Microbial Community Composition of Finish" by Allison L. Knoell, Christopher L. Anderson et al.
Microbial community analysis via 16S tag sequencing, displayed structuring of microbial communities (Bacteria and Archaea) by ... Microbial community analysis via 16S tag sequencing, displayed structuring of microbial communities (Bacteria and Archaea) by ... Home , Animal Science , Nebraska Beef Cattle Reports , 888 Animal Science Department Nebraska Beef Cattle Reports. ...http://digitalcommons.unl.edu/animalscinbcr/888/
"CHARACTERIZATION OF THE SERO-REACTIVITY OF PROTEINS MAP1152 AND MAP115" by Avery L. Paulson
Maltose-binding protein (MBP) tagged recombinant MAP proteins were purified from Escherichia coli. Western immunoblotting ... Development of genetic tools and completion of the MAP genome sequencing project expanded opportunities for antigen discovery. ... Development of genetic tools and completion of the MAP genome sequencing project expanded opportunities for antigen discovery. ... Home , Veterinary and Biomedical Sciences , Vet & Biomed Science theses , 6 Veterinary and Biomedical Sciences, Department of. ...https://digitalcommons.unl.edu/vetscidiss/6/
"PRNP Haplotype Associated with Classical BSE Incidence in European Ho" by Brenda M. Murdoch, Michael L. Clawson et al.
A combination of Illumina goldengate assay, sequencing and PCR amplification was used to genotype 18 htSNPs and 2 indels in 95 ... Methodology/Principal Findings: In this study, haplotype tagging single nucleotide polymorphisms (htSNPs) within PRNP were used ... Methodology/Principal Findings: In this study, haplotype tagging single nucleotide polymorphisms (htSNPs) within PRNP were used ... Home , USDA-ARS , MARC , 286 U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska. Roman L. Hruska ...https://digitalcommons.unl.edu/hruskareports/286/
"Abiotic Stress Responses in Photosynthetic Organisms" by Joseph Msanne
3-MA induced TAG accumulation in cells growing in both nitrogen-deprived and control media. The increase in TAG content in ... In addition to TAG accumulation, nitrogen depletion triggered an early synthesis of starch and up-regulation of several genes ... Protein degradation in nitrogen-deprived cells might provide carbon skeletons for TAG biosynthesis. In a related study, the ... TAG). Since microalgae have emerged as suitable feedstocks for renewable biofuel production, the purpose of this analysis was ...https://digitalcommons.unl.edu/natresdiss/37/
"Functional Genomic Analyses of Switchgrass Developmental Processes" by Nathan Palmer
This study added about 30,000 new expressed-sequence tags to the public databases and provided the first details on the ... This study added about 30,000 new expressed-sequence tags to the public databases and provided the first details on the ... Home , Biochemistry , Biochemistry theses , 20 Biochemistry, Department of. Theses and Dissertations in Biochemistry. ...https://digitalcommons.unl.edu/biochemdiss/20/
"A thraustochytrid diacylglycerol acyltransferase 2 with broad substra" by Chunyu Zhang, Umidjon Iskandarov et al.
45-50% identity). Recombinant TaDGAT2 restored TAG biosynthesis to the Saccharomyces cerevisiae H1246 TAG-deficient mutant, and ... The deduced TaDGAT2 sequence contains a ~460 amino acid domain most closely related to DGAT2s from Dictyostelium sp. ( ... is influenced in part by the fatty acid composition of host cells and occurs not by enhancing oleic acid content at the TAG sn- ... TAG) biosynthesis and is an important determinant of cellular oil content and quality. In this study, a gene, designated ...https://digitalcommons.unl.edu/plantscifacpub/149/
"The Nuclear Localization of the Arabidopsis Transcription Factor TIP I" by Tao Ren, Feng Qu et al.
The subcellular localization of TIP was also investigated by transiently expressing green fluorescence protein (GFP)-tagged TIP ... Secondly, we have performed gel shift experiments demonstrating that TIP does not bind to DNA in a sequence-specific manner. ... in Nicotiana benthamiana plant cells, which showed that GFP-tagged TIP localizes primarily to nuclei. Significantly, co- ... Secondly, we have performed gel shift experiments demonstrating that TIP does not bind to DNA in a sequence-specific manner. ...http://digitalcommons.unl.edu/virologypub/126/