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*  New Saccharomyces Sequences 04/27/95
SCU22109 U22109 1948bp DNA PLN 26-APR-1995 Saccharomyces cerevisiae Ytp1p (YTP1) gene, complete cds. YTP1; Ytp1p. SCU22156 U22156 3680bp DNA PLN 26-APR-1995 Saccharomyces cerevisiae Hfm1p (HFM1) gene, complete cds. HFM1; Hfm1p. SCU22361 U22361 5599bp DNA PLN 26-APR-1995 Saccharomyces cerevisiae Rlr1p (RLR1) gene, complete cds. RLR1; Rlr1p. SCU23811 U23811 1494bp DNA PLN 26-APR-1995 Saccharomyces cerevisiae RNA polymerase II holoenzyme component (SRB7) gene, complete cds. SRB7; RNA polymerase II holoenzyme component. SCU23812 U23812 4849bp DNA PLN 26-APR-1995 Saccharomyces cerevisiae RNA polymerase II holoenzyme component (SRB9) gene, complete cds. SRB9; RNA polymerase II holoenzyme component. SCU24129 U24129 2965bp DNA PLN 26-APR-1995 Saccharomyces cerevisiae sporulation-specific septin (SPR3) gene, complete ...
http://www.bio.net/bionet/mm/yeast/1995-April/002850.html
*  Saccharomyces cerevisiae DNA polymerase delta : high fidelity for base substitutions but lower fidelity for single- and multi...
Base Sequence, DNA Polymerase II/chemistry, DNA Polymerase III/*chemistry/metabolism, DNA Replication, Molecular Sequence Data, Saccharomyces cerevisiae/enzymology/*genetics, Saccharomyces cerevisiae Proteins/*chemistry ...
http://umu.diva-portal.org/smash/record.jsf?pid=diva2:146023
*  Frontiers | Effect of Temperature on the Prevalence of Saccharomyces Non cerevisiae Species against a S. cerevisiae Wine Strain...
Saccharomyces cerevisiae is the main microorganism responsible for the fermentation of wine. Nevertheless, in the last years wineries are facing new challenges due to current market demands and climate change effects on the wine quality. New yeast starters formed by non-conventional Saccharomyces species (such as S. uvarum or S. kudriavzevii) or their hybrids (S. cerevisiae x S. uvarum and S. cerevisiae x S. kudriavzevii) can contribute to solve some of these challenges. They exhibit good fermentative capabilities at low temperatures, producing wines with lower alcohol and higher glycerol amounts. However S. cerevisiae can competitively displace other yeast species from wine fermentations, therefore the use of these new starters requires an analysis of their behaviour during competition with S. cerevisiae during wine fermentation. In the present study we analyzed the survival capacity of ...
https://www.frontiersin.org/articles/10.3389/fmicb.2017.00150/full
*  "Method for Instant Saccharomyces cerevisiae Kill of Samples" by Melissa Kohner and Sara Dick
It is essential when studying the circadian rhythm in cells to be able to effectively stop them in time. In this experiment, we tested what would be the most successful killing agent on Saccharomyces cerevisiae. Six different agents were tested at different concentrations and amounts. After the S. cerevisiae was added to the test tube containing the agent, it was streaked on a plate after 5 and 10 minutes. The plates were incubated and then checked for growth. Ethanol was the most efficient killing agent. After an effective killing agent is determined, it can be used in further experiments measuring Gapdehydrogenase activity using a colorimetric assay to examine the circadian rhythm in Saccharomyces cerevisiae. Gapdehydrogenase results will also be presented.
https://scholar.valpo.edu/cus/248/
*  New Saccharomyces Sequences 02/05/00
New DNA Sequences ======================= AF200324 AF200324 579bp DNA PLN 03-FEB-2000 Saccharomyces cerevisiae Tim18p (TIM18) gene, complete cds; nuclear gene for mitochondrial product. TIM18; Tim18p. =========== Updated Sequence Features/Annotations ============= D37948 D37948 1777bp DNA PLN 01-FEB-2000 Saccharomyces cerevisiae DNA for F1F0-ATPase alpha subunit precursor, complete cds. ATP1; F1F0-ATPase alpha subunit precursor; F1F0-ATPase alpha subunit. D37949 D37949 1778bp DNA PLN 01-FEB-2000 Saccharomyces cerevisiae DNA for defective F1F0-ATPase alpha subunit precursor, complete cds. F1F0-ATPase alphadefective F1F0-ATPase alpha subunit precursor; defective F1F0-ATPase alpha subunit. YSC9SF D00347 870bp DNA PLN 01-FEB-2000 Saccharomyces cerevisiae 9kDa stabilizing factor. ATP synthase; ATP synthase inhibitor protein; mitochondria; 9kDa stabilizing factor mature protein. ...
http://www.bio.net/bionet/mm/yeast/2000-February/009235.html
*  A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during...
Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose mixtures and N-limited chemostats with varying sugar concentrations were performed. Sugar utilisation and metabolite production during fermentation were monitored. YCR17 exhibited a faster xylose consumption rate than YUSM ...
http://scholar.sun.ac.za/handle/10019.1/11872
*  Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
Recently, a new type of hybrid resulting from the hybridization between Saccharomyces cerevisiae and Saccharomyces kudriavzevii was described. These strains exhibit physiological properties of potential biotechnological interest. A preliminary characterization of these hybrids showed a trend to reduce the S. kudriavzevii fraction of the hybrid genome. We characterized the genomic constitution of several wine S. cerevisiae × S. kudriavzevii strains by using a combined approach based on the restriction fragment length polymorphism analysis of gene regions, comparative genome hybridizations with S. cerevisiae DNA arrays, ploidy analysis, and gene dose determination by quantitative real-time PCR. The high similarity in the genome structures of the S. cerevisiae × S. kudriavzevii hybrids under study indicates that they originated from a single hybridization event. After hybridization, the hybrid genome underwent ...
http://roderic.uv.es/handle/10550/31687
*  In vitro Fermentation Characteristics and Rumen Microbial Population of Diet Supplemented with Saccharomyces cerevisiae and...
Abstract: The objective of this study was to select three strains of probiotic Saccharomyces cerevisiae and to evaluate the effect of S. cerevisiae and rumen bacteria isolate (MR4) supplementation and their combination on rumen fermentability and rumen microbial population. Experiment 1 was designed in a 4 x 5 factorial randomized block design with 3 replications. The first factor was S. cerevisiae strain consisted of control treatment (without S. cerevisiae supplementation), NBRC 10217, NRRL Y 567 and NRRL 12618, and the second factor was incubation time consisted of 0, 1, 2, 3, and 4 h. Ration was basal ration for feedlot with forage to concentrate ratio (F:C)= 60:40. Dosage of each treatment with S. cerevisiae was 5 x 1010 cfu/kg ration. Experiment 2 was designed in randomized block design with 4 treatments: P0= basal ration of feedlot; P1= P0 + S. cerevisiae; P2= P0 + MR4 isolate (5 x ...
http://www.e-jurnal.com/2016/11/in-vitro-fermentation-characteristics.html
*  Cells | Free Full-Text | Assays to Monitor Autophagy in Saccharomyces cerevisiae
Autophagy is an intracellular process responsible for the degradation and recycling of cytoplasmic components. It selectively removes harmful cellular material and enables the cell to survive starvation by mobilizing nutrients via the bulk degradation of cytoplasmic components. While research over the last decades has led to the discovery of the key factors involved in autophagy, the pathway is not yet completely understood. The first studies of autophagy on a molecular level were conducted in the yeast Saccharomyces cerevisiae. Building up on these studies, many homologs have been found in higher eukaryotes. Yeast remains a highly relevant model organism for studying autophagy, with a wide range of established methods to elucidate the molecular details of the autophagy pathway. In this review, we provide an overview of methods to study both selective and bulk autophagy, including intermediate steps in the yeast Saccharomyces cerevisiae. We ...
http://www.mdpi.com/2073-4409/6/3/23
*  Identification of replication factor C from Saccharomyces cerevisiae: a component of the leading-strand DNA replication complex...
A number of proteins have been isolated from human cells on the basis of their ability to support DNA replication in vitro of the simian virus 40 (SV40) origin of DNA replication. One such protein, replication factor C (RFC), functions with the proliferating cell nuclear antigen (PCNA), replication protein A (RPA), and DNA polymerase delta to synthesize the leading strand at a replication fork. To determine whether these proteins perform similar roles during replication of DNA from origins in cellular chromosomes, we have begun to characterize functionally homologous proteins from the yeast Saccharomyces cerevisiae. RFC from S. cerevisiae was purified by its ability to stimulate yeast DNA polymerase delta on a primed single-stranded DNA template in the presence of yeast PCNA and RPA. Like its human-cell counterpart, RFC from S. cerevisiae (scRFC) has an associated DNA-activated ATPase activity as well as a primer-template, structure-specific ...
http://repository.cshl.edu/24978/
*  Saccharomyces cerevisiae afr1 protein is a protein phosphatase 1/glc7-targeting subunit that regulates the septin cytoskeleton...
Article Saccharomyces cerevisiae afr1 protein is a protein phosphatase 1/glc7-targeting subunit that regulates the septin cytoskeleton during mating. Glc7, the type1 serine/threonine phosphatase in the yeast Saccharomyces cerevisiae, is targeted by a...
https://www.environmental-expert.com/articles/saccharomyces-cerevisiae-afr1-protein-is-a-protein-phosphatase-1-glc7-targeting-subunit-that-regulat-36468
*  MIG1 overexpression causes flocculation in Saccharomyces cerevisiae. An important role of glutathione and gamma...
MIG1 overexpression causes flocculation in Saccharomyces cerevisiae. An important role of glutathione and gamma-glutamyltranspeptidase in the supply of growth requirements during nitrogen starvation of the yeast Saccharomyces cerevisiae
http://www.readabstracts.com/Biological-sciences/MIG1-overexpression-causes-flocculation-in-Saccharomyces-cerevisiae.html
*  Pet191 is a cytochrome c oxidase assembly factor in saccharomyces cerevisiae on Environmental XPRT
Article Pet191 is a cytochrome c oxidase assembly factor in saccharomyces cerevisiae. The twin-Cx9C motif protein Pet191 is essential for cytochrome c oxidase maturation. The motif Cys residues are functionally important and appear to be present in d...
https://www.environmental-expert.com/articles/pet191-is-a-cytochrome-c-oxidase-assembly-factor-in-saccharomyces-cerevisiae-36494
*  Comparative lipidomic profiling of Saccharomyces cerevisiae to reveal lipid composition changes in the plasma membrane upon...
During pretreatment of lignocellulose raw material, compounds that severely inhibit microbial activity including Saccharomyces cerevisiae strains are released [1]. These compounds, which include furaldehydes and weak organic acids, inhibit yeast metabolism and affect yeast viability and, as a consequence, reduces the overall productivity of an ethanol production process [2]. Elucidation of the molecular mechanisms behind inhibition can suggest new strategies to prevent the inhibitory effect. In the present study, the possible effect on the plasma membrane in S. cerevisiae is studied as a response to inhibitors present in lignocellulose raw material. A comparative lipidomic profiling will be carried out on S. cerevisiae cultured in the absence and presence of lignocellulose inhibitors. LC-CAD and GC-MS will be used to extensively characterize the composition of the plasma membrane. Changes in membrane composition will be correlated with the ...
http://publications.lib.chalmers.se/publication/170576-comparative-lipidomic-profiling-of-saccharomyces-cerevisiae-to-reveal-lipid-composition-changes-in-t
*  A Method to Discriminate Between the Candida stellata and Saccharomyces cerevisiae in Mixed Fermentation on WLD and Lysine Agar...
This paper presents a simple method to distinguish between Candida stellata and Saccharomyces cerevisiae yeasts during microbiological analyses. The method is based on differential yeast growth on a medium containing cycloheximide and a medium containing lysine as only nitrogen source (lysine agar). The cycloheximide resistance of 45 yeast strains belonging to Candida stellata, Hanseniaspora uvarum, Hanseniaspora guilliermondii, Metschnikowia pulcherrima, Torulaspora delbrueckii, Zygosaccharomy
https://www.sasev.org/journal/list-of-journals/a-method-to-discriminate-between-the-candida-stellata-and-saccharomyces-cerevisiae-in-mixed-fermentation-on-wld-and-lysine-agar-media/?id=14&entryId=117
*  Experimental design trade-offs for gene regulatory network inference: an in silico study of the yeast Saccharomyces cerevisiae...
en] Time-series of high throughput gene sequencing data intended for gene regulatory network (GRN) inference are often short due to the high costs of sampling cell systems. Moreover, experimentalists lack a set of quantitative guidelines that prescribe the minimal number of samples required to infer a reliable GRN model. We study the temporal resolution of data vs.quality of GRN inference in order to ultimately overcome this deficit. The evolution of a Markovian jump process model for the Ras/cAMP/PKA pathway of proteins and metabolites in the G1 phase of the Saccharomyces cerevisiae cell cycle is sampled at a number of different rates. For each time-series we infer a linear regression model of the GRN using the LASSO method. The inferred network topology is evaluated in terms of the area under the precision-recall curve (AUPR). By plotting the AUPR against the number of samples, we show that the trade-off has a, roughly speaking, sigmoid shape. An optimal number of samples ...
http://publications.uni.lu/handle/10993/33833
*  Global and Chinese HK/Hexokinase from Saccharomyces cerevisiae (CAS 9001-51-8) Industry, 2016 Market Research Report :...
[150 Pages Report] Check for Discount on Global and Chinese HK/Hexokinase from Saccharomyces cerevisiae (CAS 9001-51-8) Industry, 2016 Market Research Report report by Prof Research. The ''Global and Chinese HK/Hexokinase from Saccharomyces cerevisiae Industry,...
http://www.reportsnreports.com/reports/592980-global-and-chinese-hk-hexokinase-from-saccharomyces-cerevisiae-cas-9001-51-8-industry-2016-market-research-report.html
*  Induction of the synthesis of an additional family of long-chain dolichols in the yeast Saccharomyces cerevisiae. Effect of...
The yeast Saccharomyces cerevisiae strain W303 synthesizes in the early logarithmic phase of growth dolichols of 14-18 isoprene residues. The analysis of the polyisoprenoids present in the stationary phase revealed an additional family which proved to be also dolichols but of 19-24 isoprene residues, constituting 39% of the total dolichols. The transfer of early logarithmic phase cells to a starvation medium lacking glucose or nitrogen resulted in the synthesis of the longer chain dolichols. The additional family of dolichols represented 13.8% and 10.3% of total dolichols in the glucose and nitrogen deficient media, respectively. The level of dolichols in yeast cells increased with the age of the cultures. Since both families of dolichols are present in stationary phase cells we postulate that the longer chain dolichols may be responsible for the physico-chemical changes in cellular membranes allowing yeast cells to adapt to nutrient deficient conditions to maintain ...
http://psjd.icm.edu.pl/psjd/element/bwmeta1.element.bwnjournal-article-abpv49i3p781kz
*  "Isolation of the gene encoding the Saccharomyces cerevisiae centromere" by Richard E. Baker and Daniel C. Masison
CP1 is a sequence-specific DNA-binding protein of the yeast Saccharomyces cerevisiae which recognizes the highly conserved DNA element I (CDEI) of yeast centromeres. We cloned and sequenced the gene encoding CP1. The gene codes for a protein of molecular weight 39,400. When expressed in Escherichia coli, the CP1 gene directed the synthesis of a CDEI-binding protein having the same gel mobility as purified yeast CP1. We have given the CP1 gene the genetic designation CEP1 (centromere protein 1). CEP1 was mapped and found to reside on chromosome X, 2.0 centimorgans from SUP4. Strains were constructed in which most of CEP1 was deleted. Such strains lacked detectable CP1 activity and were viable; however, CEP1 gene disruption resulted in a 35% increase in cell doubling time and a ninefold increase in the rate of mitotic chromosome loss. An unexpected consequence of CP1 gene disruption was methionine auxotrophy genetically linked to cep1. This result and the recent finding that ...
http://escholarship.umassmed.edu/gsbs_sp/70/
*  The ribosomal protein gene RPS3 is an essential single copy gene of the yeast Saccharomyces cerevisiae. - Semantic Scholar
The communication reports the cloning, sequencing, and analysis of the RPS3 gene from yeast, which codes for the ribosomal protein YS3. Sequence analyses of a 2.45 kb DNA fragment revealed an open reading frame with the potential to code for a 240 amino-acid long protein. The first 20 amino acids display a 90% identity to a 20 amino-acid long protein sequence of yeast ribosomal protein S3, that was obtained by protein sequencing of purified yeast ribosomal proteins. The promoter region of the RPS3 gene contains several upstream conserved sequence elements (UASrpg, T-rich region) that usually regulate transcription of ribosomal protein genes. Northern blot experiments demonstrate that this ORF is transcribed into an approximately 900 nt long mRNA. The major start site of transcription is located near position -20. The RPS3 gene is a single copy gene in yeast. Its disruption yields non viable haploid spores of Saccharomyces cerevisiae.
https://www.semanticscholar.org/paper/The-ribosomal-protein-gene-RPS3-is-an-essential-si-Fingen-Eigen-Domdey/2211a2135b0aa96285783d68b19d250f4a87b476
*  Expression of the Aspergillus niger InuA gene in Saccharomyces cerevisiae permits growth on the plant storage carbohydrate...
The plant storage carbohydrate inulin represents an attractive biomass feedstock for fueling industrial scale bioconversion processes due to its low cost, ability for cultivation on arid and semi-arid lands, and amenability to consolidated bioprocessing applications. As a result, increasing efforts are emerging towards engineering industrially relevant microorganisms, such as yeast, to efficiently ferment inulin into high value fuels and chemicals. Although some strains of the industrially relevant yeast model Saccharomyces cerevisiae can naturally ferment inulin, the efficiency of this process is often supplemented through expression of exogenous inulinase enzymes that externally convert inulin into its more easily fermentable component monomeric sugars. Here, the effects of overexpressing the Aspergillus niger InuA inulinase enzyme in an S. cerevisiae strain incapable of endogenously fermenting inulin were evaluated to determine their impact on growth. ...
https://www.osti.gov/scitech/biblio/1265289-expression-aspergillus-niger-inua-gene-saccharomyces-cerevisiae-permits-growth-plant-storage-carbohydrate-inulin-low-enzymatic-concentrations
*  Electron-microscopic study of the spindle and chromosome movement in the yeast Saccharomyces cerevisiae | Journal of Cell...
Mitosis in yeast Saccharomyces cerevisiae was investigated in thick (0-25-I mum) serial sections with a high voltage electron microscope and in preparations of spheroplasts spread on a water surface. Spindle microtubules originate from a plaque-like structure called the spindle pole bosis the SPB duplicates and a set of long and short microtubules develops on each SPB. The spindle arises as the SPBs separate on the nuclear membrane adense and are not individually visible. Genetic studies, however, have indicated that there are 17 linkage groups. The number of microtubules was determined in diploid and haploid spindles on serial stereo micrographs. In diploid mitosis about 40 microtubules issue from a SPB. Most are non-continuous and often they are visibly associated with a chromatin fibre. The spindle in haploid cells is similar except that the number of microtubules is about half that in diploid cells and the SPB is smaller. The pole-to-pole microtubules vary in number from ...
http://jcs.biologists.org/content/22/2/219
*  Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris | Biochemical Journal
In Saccharomyces cerevisiae the activity for the lactate-proton symporter is dependent on JEN1 gene expression. Pichia pastoris was transformed with an integrative plasmid containing the JEN1 gene. After 24 h of methanol induction, Northern and Western blotting analyses indicated the expression of JEN1 in the transformants. Lactate permease activity was obtained in P. pastoris cells with a Vmax of 2.1 nmol·s−1·mg of dry weight−1. Reconstitution of the lactate permease activity was achieved by fusing plasma membranes of P. pastoris methanol-induced cells with Escherichia coli liposomes containing cytochrome c oxidase, as proton-motive force. These assays in reconstituted heterologous P. pastoris membrane vesicles demonstrate that S. cerevisiae Jen1p is a functional lactate transporter. Moreover, a S. cerevisiae strain deleted in the JEN1 gene was transformed with a centromeric plasmid containing JEN1 under the control of the ...
http://www.biochemj.org/content/376/3/781
*  Cell death with predominant apoptotic features in Saccharomyces cerevisiae mediated by deletion of the histone chaperone ASF1...
Background Although no potential homologues of multicellular apoptotic genes (e.g. Bax, Bak, Bcl-2, caspases and p53) have been identified in a unicellular eukaryote, previous reports contain several implications of the apoptotic behaviour of yeasts (i.e. Saccharomyces cerevisiae and Schizosaccharomyces pombe). Therefore, whether or not yeast undergoes apoptosis has been a topic of some debate. hCCG1, which is the largest subunit of TFIID and a histone acetyltransferase, appears to be involved in the regulation of apoptosis. The factor hCIA interacts with hCCG1 and functions as a histone chaperone in mammalian cells; its homologue in yeast is Asf1p/Cia1p. Therefore, we anticipated that a yeast mutant in Asf1p/Cia1p would be a valuable tool for studying apoptosis in yeast.. Results We established a strain of S. cerevisiae lacking the histone chaperone ASF1/CIA1. This disruptant, asf1/cia1, arrested preferentially at the G2/M-phase and died. ...
http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2443.2001.00487.x/abstract?globalMessage=0
*  Effect of Mutations in Genes PH085 and PH04 on Proline Utilization in Yeast Saccharomyces cerevisiae, Russian Journal of...
Read "Effect of Mutations in Genes PH085 and PH04 on Proline Utilization in Yeast Saccharomyces cerevisiae, Russian Journal of Genetics" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
https://www.deepdyve.com/lp/springer_journal/effect-of-mutations-in-genes-ph085-and-ph04-on-proline-utilization-in-0oUegg0Dpu