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*  Vaccines | Free Full-Text | Replicon RNA Viral Vectors as Vaccines
Replicon RNA vectors have also been subjected to clinical trials. Overall, immunization with self-replicating RNA viruses ... Immunization of mice, chicken, pigs and primates with virus-like particles, naked RNA or layered DNA/RNA plasmids has provided ... Administration of replicon RNA vectors has resulted in strong immune responses and generation of neutralizing antibodies in ... Moreover, recombinant particles and replicon RNAs have been encapsulated by liposomes to improve delivery and targeting. ...
http://www.mdpi.com/2076-393X/4/4/39
*  Viral RNA Replication Modes: Evolutionary and Dynamical Implications | SpringerLink
Sardanyés J. (2014) Viral RNA Replication Modes: Evolutionary and Dynamical Implications. In: Corral Á., Deluca A., Font-Clos F ... J. Sardanyés, F. Martínez, J.A. Daròs, S.F. Elena, Dynamics of alternative modes of RNA replication for positive-sense RNA ... L. Chao, C.U. Rang, L.E. Wong, Distribution of spontaneous mutants and inferences about the replication mode of the RNA ... F. Martínez, J. Sardanyés, J.A. Daròs, S.F. Elena, Dynamics of a plant RNA virus intracellular accumulation: stamping machine ...
https://link.springer.com/chapter/10.1007/978-3-319-08138-0_21
*  quantitative viral RNA assay - Molecular Biology
quantitative viral RNA assay - (Jun/08/2001 ). I'd like to determine viral RNA level changes in virus producing cell culture ...
http://www.protocol-online.org/biology-forums/posts/955.html
*  The Effectiveness of HIV RNA Viral Load Testing in Determining Treatment Type in HIV-Infected Patients - Full Text View -...
The Effectiveness of HIV RNA Viral Load Testing in Determining Treatment Type in HIV-Infected Patients. This study has been ... A Randomized Controlled Clinical Study to Determine If the Addition of HIV RNA Viral Load Is an Effective Tool in Determining ... RNA Virus Infections. Virus Diseases. Sexually Transmitted Diseases, Viral. Sexually Transmitted Diseases. Immunologic ... whether an antiretroviral treatment regimen based upon clinical evaluation and CD4 counts plus HIV RNA viral load is more ...
https://clinicaltrials.gov/show/NCT00002376?order=323
*  Zika virus infection alters human and viral RNA | EurekAlert! Science News
These modifications -- chemical tags known as methyl groups -- influence viral replication and the human immune response. ... of California San Diego School of Medicine have discovered that Zika virus infection leads to modifications of both viral and ... the researchers removed the human enzymes responsible for adding methyl groups to viral RNA. Without m6A, the viral RNA was ... the cell modifies viral RNA with m6A as a means to get rid of the infection. RNA tagged with m6A is a beacon for human enzymes ...
https://www.eurekalert.org/pub_releases/2016-10/uoc--zvi101816.php
*  Link uncovered between viral RNA and human immune response
Bevilacqua »HIV-1 genome »Molecular Biology »PKR »RNA »RNA dimers »TAR »double-stranded RNAs »genetic material »human cells » ... Further reports about: , Bevilacqua , HIV-1 genome , Molecular Biology , PKR , RNA , RNA dimers , TAR , double-stranded RNAs , ... One way for this to happen is for the viral RNA to first form linked pairs called dimers. These RNA dimers then allow separate ... Link uncovered between viral RNA and human immune response. 06.08.2009. In its fight against an intruding virus, an enzyme in ...
http://www.innovations-report.com/html/reports/life-sciences/link-uncovered-viral-rna-human-immune-response-137329.html
*  Imaging Technique Captures RNA Viral Infections in Progress | Science and Enterprise
... and Vanderbilt University devised a method to follow viral infections without affecting the virus or its host. ... A super-resolution optical image of a single viral protein filament, in red, with viral RNA, in green (Eric Alonas and Philip ... The respiratory syncytial virus also uses ribonucleic acid or RNA as its viral material to infect healthy host cells, which ... The technique combines short DNA and RNA strands with the fluorescent chemicals to become a protein that binds to the nucleic ...
https://sciencebusiness.technewslit.com/?p=16376
*  Microbial Transcriptomics | Bacterial and viral RNA-Seq
RNA-Seq) offers an unbiased approach for analyzing and quantifying bacterial, viral, and other microbial transcripts. ... RNA-Seq for Microbial Transcript Analysis. Bacterial, viral, and other microbial RNA-Seq experiments enable annotation and ... Note: If using the TruSeq RNA library prep kit, you will NOT need to extract RNA and transcribe to cDNA. If you use an RNA ... Streamlined, cost-efficient, and scalable solution for total RNA analysis.. Note: If you use a TruSeq RNA library prep kit, you ...
https://www.illumina.com/areas-of-interest/microbiology/microbial-sequencing-methods/microbial-transcriptomics.html
*  PureLink Viral RNA/DNA Mini Kit - Thermo Fisher Scientific
The PureLink Viral Mini Kit is specifically designed to isolate high-q ... The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or ... The PureLink® Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or ... The PureLink® Viral RNA/DNA Mini Kit contains enough reagents for 50 reactions.. Kit Contents:. • 32 ml Viral Lysis buffer (L22 ...
https://www.thermofisher.com/order/catalog/product/12280050
*  Viral RNA/DNA Purification | Thermo Fisher Scientific - US
Viral RNA and DNA capture, purification and qRT-PCR and qPCR analyses; featuring PureLink™, RNA UltraSense™, & Dynabeads® ... Specific Capture of Viral RNA/DNA Target a specific viral RNA or DNA sequence and capture only that specific nucleic acid ... Dynabeads® SILANE Viral RNA/DNA Sensitive and highly consistent isolation of viral RNA and DNA from cell-free samples. These ... Viral RNA/DNA purification Both spin-column and 96-well plate kits are designed for fast and easy isolation of viral RNA or DNA ...
https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/viral-rna-dna-purification.html
*  Application # 2007/0265222. Nucleoside Aryl Phosphoramidates for the Treatment of Rna-Dependent Rna Viral Infection -...
These compounds are precursors to inhibitors of RNA-dependent ... which are precursors to inhibitors of RNA-dependent RNA viral ... inhibiting RNA-dependent RNA viral replication, and/or a method for treating RNA-dependent RNA viral infection in a mammal in ... RNA-dependent RNA viral polymerase is a positive-sense single-stranded RNA-dependent RNA viral polymerase. In a class of this ... RNA-dependent RNA viral replication is a positive-sense single-stranded RNA-dependent RNA viral replication. In a class of this ...
http://patents.com/us-20070265222.html
*  Clinical Prognostic Value of RNA Viral Load and CD4 Cell Counts during Untreated HIV-1 Infection-A Quantitative Review
Comparing a patient with RNA at upper 75th centile with a patient at median RNA, 5-year relative risks were 1.4 (95% CI 1.2-1.7 ... Median within-population interquartile ranges were 74,000 copies/mL for RNA with no significant change during the course of ... without pre-treatment RNA monitoring - while not precluding earlier treatment based on clinical, socio-demographic or public ... of population-level variation in RNA, and 25% of variation in CD4, were due to within-patient fluctuations. ...
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0005950
*  A Viral RNA Structural Element Alters Host Recognition of Nonself RNA | Science
A Viral RNA Structural Element Alters Host Recognition of Nonself RNA Message Subject. (Your Name) has forwarded a page to you ... A Viral RNA Structural Element Alters Host Recognition of Nonself RNA. By Jennifer L. Hyde, Christina L. Gardner, Taishi Kimura ... A Viral RNA Structural Element Alters Host Recognition of Nonself RNA. By Jennifer L. Hyde, Christina L. Gardner, Taishi Kimura ... As an example, 2'-O methylation of the 5′ cap of viral RNA subverts mammalian antiviral responses by evading restriction of ...
http://science.sciencemag.org/content/early/2014/01/29/science.1248465
*  Nuclear Export of Viral RNAs by Joachim Hauber, P. K. Vogt | Waterstones
Buy Nuclear Export of Viral RNAs by Joachim Hauber, P. K. Vogt from Waterstones today! Click and Collect from your local ... Nuclear Export of Viral RNAs - Current Topics in Microbiology and Immunology 259 (Paperback). Joachim Hauber (editor), P. K. ... CRM I appears to be involved in the nucleocytoplasmic translocation of the vast majority of viral and cellular proteins that ...
https://www.waterstones.com/book/nuclear-export-of-viral-rnas/joachim-hauber/p-k-vogt/9783642625220
*  DIGITAL.CSIC: A comparison of viral RNA-dependent RNA polymerases
A comparison of viral RNA-dependent RNA polymerases. Autor : Ferrer-Orta, Cristina ; Arias, Armando; Escarmís, Cristina; ... Comparing these data with results from previous structural analyses of viral RNA-dependent RNA polymerases that catalyze de ... Genome replication in picornaviruses is catalyzed by a virally encoded RNA-dependent RNA polymerase, termed 3D. These viruses ... The recent explosion of structural information on picornaviral 3D polymerases has provided insights into the initiation of RNA ...
https://digital.csic.es/handle/10261/110069
*  Plasma RNA viral load is not associated with intrapatient quasispecies heterogeneity in HIV-1 infection, Archives of Virology |...
"Plasma RNA viral load is not associated with intrapatient quasispecies heterogeneity in HIV-1 infection, Archives of Virology" ... Plasma RNA viral load is not associated with intrapatient quasispecies heterogeneity in HIV-1 infection. Plasma RNA viral load ... In this study, we analyzed the association between intrapatient viral diversity and RNA viral load in 16 antiretroviral therapy ... In this study, we analyzed the association between intrapatient viral diversity and RNA viral load in 16 antiretroviral therapy ...
https://www.deepdyve.com/lp/springer_journal/plasma-rna-viral-load-is-not-associated-with-intrapatient-quasispecies-s6tjZh0vCP
*  Exploring Methods for the Characterization of Viral RNA-protein Complexes
The viral genome is composed of two viral RNA's: RNA 1 and RNA 2. Deletion and mutation studies in the N- and C-terminus of ... The C-terminus is needed for packaging of both viral RNAs. The identified protein regions involved in packaging viral RNAs bind ... A well studied RNA-protein interaction, the HIV-1 Rev-RRE, was used to develop the methodology for the surface based system. ... protein alpha have identified protein regions required for the packaging of FHV viral RNAs. Residues 2-31 on the N-terminus ...
https://uwspace.uwaterloo.ca/handle/10012/4718
*  Structure Cluster - 1L2X: Atomic Resolution Crystal Structure of a Viral RNA Pseudoknot 3D Similarity Report Page
Metal ions and flexibility in a viral RNA pseudoknot at atomic resolution. ... Description: RNA pseudoknot rna , Length: 28 This entity is NOT a polypeptide entity and therefore cannot be considered for the ...
http://www.rcsb.org/pdb/explore/structureCluster.do?structureId=1L2X
*  IDEALS @ Illinois: Single molecule pull-down of viral RNA-protein complexes
viral RNA total internal reflection fluorescence microscopy Ribonucleic acid (RNA). Abstract:. Protein and RNA molecules ... The viral DI RNA associates with multiple viral proteins during replication, and is therefore expected to form heterogeneous ... Using the RNA sequence information of DI RNA, we designed several short complementary DNA probes to capture single DI RNA ... Utilizing a biological system of virally infected mammalian cells, the substrate targeted in the study are viral RNA-protein ...
https://www.ideals.illinois.edu/handle/2142/31924
*  Isolation of poliovirus 2C mutants defective in viral RNA synthesis. | Journal of Virology
... a drug whose action also affects viral RNA synthesis. Thus, protein 2C is one that is needed continually for viral RNA ... Both mutants have a severe temperature-sensitive defect in viral RNA synthesis but little effect on the types of viral protein ... Isolation of poliovirus 2C mutants defective in viral RNA synthesis. Message Subject (Your Name) has forwarded a page to you ... Isolation of poliovirus 2C mutants defective in viral RNA synthesis.. J P Li, D Baltimore ...
https://jvi.asm.org/content/62/11/4016
*  ASMscience | Viral RNA Synthesis
... models that have been proposed to explain how viral positive and negative RNA species are made by the viral RNA-dependent RNA ... It specifically provides the roles of viral proteins and RNA sequences in RNA replication, and describes the kinetics and ... and negative-strand RNA synthesis, the source of specificity for the viral template RNA, and the relationship between ... enteroviruses encode several proteins required for viral RNA synthesis. Open questions about the mechanism of viral synthesis ...
http://www.asmscience.org/content/book/10.1128/9781555818326.chap4
*  The ESCRT-I Subunit TSG101 Controls Endosome-to-Cytosol Release of Viral RNA
... is dispensable for viral envelope fusion with endosomal membranes and viral RNA transport to late endosomes but is necessary ... The ESCRT-I Subunit TSG101 Controls Endosome-to-Cytosol Release of Viral RNA. ... There, the viral envelope undergoes fusion with endosomal membranes, thereby releasing the nucleocapsid into the cytoplasm and ... Previously, we reported that the viral envelope fuses preferentially with the membrane of vesicles present within ...
https://insights.ovid.com/traff/200812000/00128667-200812000-00022
*  Preference of RIG-I for short viral RNA molecules in infected cells revealed by next-generation sequencing | PNAS
We conclude that viral replicating RNA constitutes the majority of the viral immunostimulatory RNA associated with RIG-I. In ... purified SeV-C RNA, total RNA from SeV-C-infected cells, and poly(I:C) were used in various experiments. Viral RNA was isolated ... or trailer RNA. Comparison of relative abundances of DI RNA and genome RNA/L mRNA between total RNA from infected cells, RIG-I ... Analysis of RIG-I-Associated RNA at Early Times of SeV Infection.. We next determined whether the same or different viral RNA ...
http://www.pnas.org/content/107/37/16303
*  BIOL 230 Lecture Guide - Production of Viral mRNA from a ss plus RNA Viral Genome
RNA viral genome. To produce viral mRNA molecules. RNA-dependent RNA polymerase enzymes copy the (-) RNA strand into (+) viral ... To replicate the viral genome, RNA-dependent RNA polymerase enzymes copy the (+) RNA genome producing ss (-) RNA. RNA-dependent ... 10E: Replication of a Single-Stranded Plus RNA Viral Genome and Production of Viral mRNA. ... The (+) viral mRNA can then be transtated into viral proteins by host cell ribosomes. ...
http://faculty.ccbcmd.edu/courses/bio141/lecguide/unit4/viruses/ssplusRNA_lc.html
*  Mutational analysis of a viral RNA element that counteracts rapid RNA decay by interaction with the polyadenylate tail | PNAS
Mutational analysis of a viral RNA element that counteracts rapid RNA decay by interaction with the polyadenylate tail. ... Mutational analysis of a viral RNA element that counteracts rapid RNA decay by interaction with the polyadenylate tail ... Mutational analysis of a viral RNA element that counteracts rapid RNA decay by interaction with the polyadenylate tail ... Mutational analysis of a viral RNA element that counteracts rapid RNA decay by interaction with the polyadenylate tail ...
https://www.pnas.org/content/104/25/10412?ijkey=b52cbdbb4b70971a13cefc3b903a4628cfd737a9&keytype2=tf_ipsecsha