Spliced leader RNA silencing (SLS) - a programmed cell death pathway in Trypanosoma brucei that is induced upon ER stress |...
... the spliced leader (SL) is added to all mRNAs from a small RNA, the SL RNA. Trypanosomes lack the machinery for the unfolded ... trypanosomes elicit the spliced leader silencing (SLS) pathway. In SLS, the transcription of the SL RNA gene is extinguished, ... splicing and polyadenylation. In trans- splicing, which is essential for processing of each mRNA, an exon, ... SLS leads to complete shut-off of trans-splicing. In this review, I discuss the UPR in mammals and compare it to the ER stress ...https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-5-107
Three Small Nucleolar RNAs Identified from the Spliced Leader-Associated RNA Locus in Kinetoplastid Protozoans | Molecular and...
1994) Identification of a small RNA that interacts with the 5′ splice site of the Trypanosoma brucei spliced leader RNA in vivo ... the spliced leader (SL) RNA (12, 30, 38), four U small nuclear RNAs involved in trans splicing (U2, U4, and U6 [39, 51, 52] and ... Three Small Nucleolar RNAs Identified from the Spliced Leader-Associated RNA Locus in Kinetoplastid Protozoans. T. Guy Roberts ... Three Small Nucleolar RNAs Identified from the Spliced Leader-Associated RNA Locus in Kinetoplastid Protozoans ...https://mcb.asm.org/content/18/8/4409
The 35-nucleotide spliced leader sequence is common to all trypanosome messenger RNA's | Science
The 35-nucleotide spliced leader sequence is common to all trypanosome messenger RNA's ... The 35-nucleotide spliced leader sequence is common to all trypanosome messenger RNA's ... The 35-nucleotide spliced leader sequence is common to all trypanosome messenger RNA's ... The 35-nucleotide spliced leader sequence is common to all trypanosome messenger RNA's ...http://science.sciencemag.org/content/233/4763/569
Spliced‐leader RNA silencing: a novel stress‐induced mechanism in Trypanosoma brucei | EMBO Reports
... the main RNA molecule is the spliced‐leader (SL) RNA, which donates the SL sequence to all messenger RNA through trans‐splicing ... Spliced‐leader RNA silencing is activated under pH stress. (A) SL RNA and mRNA reduction under low pH. (a) RNA was prepared ... SL RNA, spliced‐leader RNA; snoRNA, small nucleolar RNA; SRα, SRP receptor α; SRP, signal‐recognition particle. ... leader RNA transcription and messenger RNA production. (A) The effect of SRα or SRP54 depletion on RNA level. RNA (20 μg) was ...http://embor.embopress.org/content/8/4/408
The Genome Sequence of Caenorhabditis briggsae: A Platform for Comparative Genomics
Spliced Leader RNA Genes. A characteristic of many C. elegans genes is the presence of a trans-spliced leader, a short RNA that ... Two types of spliced leader, SL1 and SL2, have been described.. The SL1 RNA gene that donates the SL1 spliced leader is present ... small nucleolar RNA; snRNA, small nuclear RNA; tRNA, transfer RNA; UTR, untranslated region; WGS, whole-genome shotgun ... Ribosomal RNA Genes. The genes for three of the four ribosomal RNA (rRNA) components, 18S, 5.8S, and 26S, are known to occur in ...http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0000045
Comparative Genomic and Transcriptomic Characterization of the Toxigenic Marine Dinoflagellate Alexandrium ostenfeldii
... trans-splicing and mRNA transposition mechanisms. Alexandrium ostenfeldii is known to produce macrocyclic imine toxins, ... 2007) Spliced leader RNA trans-splicing in dinoflagellates. Proceedings of The National Academy of Sciences of The United ... Lidie K, Van Dolah F (2007) Spliced leader RNA-mediated trans-splicing in a dinoflagellate, Karenia brevis. Journal of ... Zhang H, Campbell DA, Sturm NR, Lin S (2009) Dinoflagellate spliced leader RNA genes display a variety of sequences and genomic ...https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0028012
Foreign genes and novel hydrophilic protein genes participate in the desiccation response of the bdelloid rotifer Adineta...
2005). Spliced leader RNA-mediated trans-splicing in phylum Rotifera. Mol. Biol. Evol. 22, 1482-1489. ... The presence of a spliced leader, found at the 5′ end of a proportion of bdelloid mRNAs (Pouchkina-Stantcheva and Tunnacliffe, ... RNA extraction. RNA was extracted using TRIzol reagent (Invitrogen, Paisley, UK) according to manufacturer's protocol, washed ... RNA was extracted from both samples; cDNA synthesis was performed for both driver (non-desiccated) and tester (desiccated) ...http://jeb.biologists.org/content/214/1/59.full
Heat shock causes a decrease in polysomes and the appearance of stress granules in trypanosomes independently of eIF2α...
Northern blot probed for total mRNA using an antisense oligonucleotide recognising the spliced leader; the spliced leader RNA ( ... Ullu, E. and Tschudi, C. (1991). Trans splicing in trypanosomes requires methylation of the 5′ end of the spliced leader RNA. ... McNally, K. P. and Agabian, N. (1992). Trypanosoma brucei spliced-leader RNA methylations are required for trans splicing in ... To investigate the fate of mRNA during heat shock, RNA was prepared and northern blots were probed for the 5′-spliced leader ...http://jcs.biologists.org/content/121/18/3002
Gemma B. Danks | University of Bergen
spliced-leader led RNA trans-splicing. *nutrient-dependent translational control. *dynamics of promoter usage ... 2015).Trans-splicing and operons in metazoans: translational control in maternally regulated development and recovery from ... Trans-splicing and operons in metazoans: Translational control in maternally regulated development and recovery from growth ... 2015). Trans-splicing in metazoans: A link to translational control? Worm. 4(3):e1046030. http://dx.doi.org/10.1080/ ...https://www.uib.no/en/persons/Gemma.Danks
Trans-Spliced Exon Coupled RNA End Determination - Wikipedia
... end of the messenger RNA require the presence of a trans-spliced leader sequence. Spliced leader sequences are short sequences ... Huang XY, Hirsh D (November 1989). "A second trans-spliced RNA leader sequence in the nematode Caenorhabditis elegans". Proc. ... Spliced leaders have been seen in trypanosomatids, Euglena, flatworms, Caenorhabditis. Some species contain only one spliced ... Total RNA is purified from the specimen of interest. Poly A messenger RNA is then purified from total RNA and subsequently ...https://en.wikipedia.org/wiki/Trans-Spliced_Exon_Coupled_RNA_End_Determination
Extensive Differences in Gene Expression Between Symbiotic and Aposymbiotic Cnidarians | G3: Genes | Genomes | Genetics
2007 Spliced leader RNA trans-splicing in dinoflagellates. Proc. Natl. Acad. Sci. USA 104: 4618-4623. ... The resulting RNA was purified using the RNA Clean and Concentrator-25 Kit (Zymo Research R1017). For RNA-Seq, the RIN of each ... In experiments 2 and 3, total RNA was extracted using the ToTALLY RNA Total RNA Isolation Kit (Ambion AM1910) following the ... RNA isolation and sequencing. In experiment 1, total RNA was extracted from whole anemones using the RNAqueous-4PCR Kit (Ambion ...http://www.g3journal.org/content/4/2/277
Operons | SpringerLink
The spliced leader sequence (black box) is derived from a spliced leader (SL) RNA which carries the 5′ RNA cap (circle) . ... In trans-splicing, a donor splice-leader RNA donates a short leader sequence to a splice acceptor site within the polycistronic ... Ganot P, Kallesoe T, Reinhardt R, Chourrout D, Thompson EM (2004) Spliced-leader RNA trans splicing in a chordate, Oikopleura ... Pouchkina-Stantcheva NN, Tunnacliffe A (2005) Spliced leader RNA-mediated trans-splicing in phylum Rotifera. Mol Biol Evol 22: ...https://link.springer.com/article/10.1007%2Fs00018-009-0114-3
Characterization of trans-splicing in Euglenoids | SpringerLink
We have looked for trans-splicing of nuclear mRNAs in several Euglenoid species. In Cyclidiopsis acus, Phacus curvicauda, ... Key words Euglenoid Trans-splicing Spliced leader-sequence RNA Euglena gracilis Received: 15 November 1999 / 10 January ... splicing process: we identified SL-RNA genes whose 5′ ends (SLs for spliced leader-sequences) were transferred to the 5′ ... The SL-RNA genes are located on repeated DNA fragments which also encode 5S rRNA in P. curvicauda and C. acus. The potential ...https://link.springer.com/article/10.1007%2Fs002940000116
The American Journal of Tropical Medicine and Hygiene | Infection Rates of Triatoma protracta (Uhler) with Trypanosoma cruzi in...
Incongruence of 24Sα ribosomal RNA phylogeny with current T. cruzi genotype classification supports non-clonality of some T. ... protracta was conducted for the first time by using partial 18S ribosomal RNA and 24Sα ribosomal RNA sequences. ... Trypanosomatid biodiversity in Costa Rica: genotyping of parasites from heteroptera using the spliced leader RNA gene. ... protracta was conducted for the first time by using partial 18S ribosomal RNA and 24Sα ribosomal RNA sequences. Incongruence of ...http://www.ajtmh.org/content/journals/10.4269/ajtmh.2010.10-0167
Trans-splicing - Wikipedia
... a capped splice leader RNA is transcribed; simultaneously, genes are transcribed in long polycistrons. The capped splice leader ... Campbell, D. A.; Sturm, N. R.; Yu, M. C. (2000-02-01). "Transcription of the kinetoplastid spliced leader RNA gene". ... Trans-splicing is a special form of RNA processing in eukaryotes where exons from two different primary RNA transcripts are ... Whereas "normal" (cis-)splicing processes a single molecule, trans-splicing generates a single RNA transcript from multiple ...https://en.wikipedia.org/wiki/Trans-splicing
Lukeš Julius | Řádní členové Učené společnosti | Členové | Učená společnost České republiky
O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma brucei. Mol. Cell. Biol. 27 ... Two sequence classes of kinetoplastid 5S ribosomal RNA gene revealed among bodonid spliced leader RNA gene arrays. FEMS Lett. ... Zeiner G., Foldynová S., Sturm N.R., Lukeš J. & Campbell D.A. (2004). SmD1 is required for spliced leader RNA biogenesis. ... Diversity of insect trypanosomatids assessed from the spliced leader RNA and 5S rRNA genes and intergenic regions. J. Euk. ...https://www.learned.cz/cz/clenove-ucene-spolecnosti/radni-clenove-ucene-spolecnosti/lukes-julius.html
Searle Scholars Program : Scott A. Strobel (1997)
We have established collaborations to map the splice leader RNA, U6 snRNA, RNaseP and the decoding site in rRNA. We will also ... an RNA component, (ii) determination of the RNA tertiary structure that defines a molecular zip code for cytoplasmic RNA ... RNA Structure and Cellular Localization Overview: The research efforts of my lab focus on the biochemical basis of RNA ... 3. Defining the Chemical Basis of RNA Structure and Catalysis. Formation of higher order RNA structure is achieved by long ...https://searlescholars.net/person/180
Christian Tschudi, PhD > MD-PhD Program | Yale School of...
Ruan JP, Shen S, Ullu E, Tschudi C: Evidence for a capping enzyme with specificity for the trypanosome spliced leader RNA. Mol ... Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference- ... Arhin GK, Ullu E, Tschudi C: 2'-O-methylation of position 2 of the trypanosome spliced leader cap 4 is mediated by a 48 kDa ... We are applying RNA interference (RNAi), namely down-regulation of gene expression by homologous double-stranded RNA, to ...https://medicine.yale.edu/mdphd/people/christian_tschudi-2.profile
Institute of Cell Biology: Prof. Dr. Torsten Ochsenreiter - University of Bern
The nuclear RNA binding protein RBP33 influences mRNA and spliced leader RNA abundance in Trypanosoma brucei. Molecular and ... Spliced leader trapping reveals widespread alternative splicing patterns in the highly dynamic transcriptome of Trypanosoma ... Hajduk, Stephen; Ochsenreiter, Torsten (2010). RNA editing in kinetoplastids. RNA biology, 7(2), pp. 229-36. Georgetown, Tex.: ... Group leader. Mail. email@example.com. Phone. +41 31 631 46 56. PhD students. Name / Titel. Hélène Baudouin ...http://www.izb.unibe.ch/research/prof_dr_torsten_ochsenreiter/index_eng.html
Control of gene expression in Trypanosomatidae
Trypanosoma cruzi strains partition into two groups based on the structure and function of the spliced leader RNA and rRNA gene ... or spliced leader, SL) that is joined to the 5' end of every mRNA in a transesterification reaction called trans-splicing. ... "cis-splicing" reactions. RNA polymerase II is the enzyme responsible for the transcription of protein-coding genes, whereas RNA ... 2. Donelson JE & Zeng W (1990). A comparison of trans-RNA splicing in trypanosomes and nematodes. Parasitology Today, 6: 327- ...http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200001&lng=en&nrm=iso
Faculty Directory › UConn Health
Individual mRNAs are then processed from precursor RNA by spliced leader (SL) trans splicing and polyadenylation. Since trans ... Trypanosome cdc2-related kinase 9 controls spliced leader RNA cap4 methylation and phosphorylation of RNA polymerase II subunit ... Spliced leader RNA gene transcription in Trypanosoma brucei requires transcription factor TFIIH. Lee, Ju Huck; Nguyen, Tu N; ... A TFIIH-associated mediator head is a basal factor of small nuclear spliced leader RNA gene transcription in early-diverged ...http://facultydirectory.uchc.edu/profile?profileId=Gunzl-Arthur
The transcriptome of the novel dinoflagellate Oxyrrhis marina (Alveolata: Dinophyceae): response to salinity examined by 454...
Zhang H, Hou Y, Miranda L, Campbell DA, Sturm NR, Gaasterland T, Lin S: Spliced leader RNA trans-splicing in dinoflagellates. ... In both cases spliced leader sequences were spliced at an 'AG' acceptor site (Figure 6).. Table 3 Oxyrrhis marina genes ... basal dinoflagellates or Perkinsus spp). In all cases 454 contigs were truncated and no spliced leader sequences  were ... For example, it is now clear that the RNA trans-splicing mechanism, seemingly ubiquitous within the dinoflagellates, also ...https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-12-519
... end to the RNA splice leader SL1 (Krause and Hirsh, 1987). The SL1-derived consensus sequence was found at the 5′ end of the ... composing the putative RNA trans-splice leader sequence (SL1). Amino acids are numbered (in italics) beginning with the first ... Trans-splicing occurs at the 5′ end of the transcript to the 22-nucleotide leader SL1 (open box). The putative polyadenylation ... 1987) Trans-spliced leader sequence on actin mRNA in C. elegans. Cell 49:753-761. ...http://www.jneurosci.org/content/18/24/10438
Senjie Lin | Marine Sciences
Spliced leader RNA trans-splicing in dinoflagellates. Proc. Natl. Acad. Sci. USA 104: 4618-4623. ... Spliced leader-based metatranscriptomic analyses lead to recognition of hidden genomic features in dinoflagellates. Proc. Natl ...https://marinesciences.uconn.edu/faculty/lin/
The Symbiodinium kawagutii genome illuminates dinoflagellate gene expression and coral symbiosis | Science
Spliced leader RNA trans-splicing in dinoflagellates. Proc. Natl. Acad. Sci. U.S.A. 104, 4618-4623 (2007). doi:10.1073/pnas. ... Proof that dinoflagellate spliced leader (DinoSL) is a useful hook for fishing dinoflagellate transcripts from mixed microbial ... Fungal small RNAs suppress plant immunity by hijacking host RNA interference pathways. Science 342, 118-123 (2013). pmid: ... We identified a double-stranded RNA (dsRNA)-gated channel protein Systemic RNA Interference Deficiency-1 (SID-1) (9) required ...https://science.sciencemag.org/content/350/6261/691?ijkey=b6d6e1211e267b744b7aa9179a01496023621ddc&keytype2=tf_ipsecsha