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*  Structural basis for CRISPR RNA-guided DNA recognition by Cascade. - Oxford Neuroscience
... and a 61-nucleotide CRISPR RNA (crRNA) with 5'-hydroxyl and 2',3'-cyclic phosphate termini. The crRNA guides Cascade to dsDNA ... immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids. In Escherichia coli, immunity ... Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM., Lundgren M., van Duijn E., Bultema JB., Westra ER ... RNA, Bacterial, RNA, Guide, Ribonucleoproteins, Structure-Activity Relationship ...
https://www.neuroscience.ox.ac.uk/publications/382766
*  Publications - Herschlag Lab
Lessons from Enzyme Kinetics Reveal Specificity Principles for RNA-Guided Nucleases in RNA Interference and CRISPR-Based Genome ... RNA Structural Modules Control the Rate and Pathway of RNA Folding and Assembly. PMCID: PMC5048535. (Medline) (PDF File) ( ... Unwinding RNA's Secrets: Advances in the Biology, Physics, and Modeling of Complex RNAs. PMCID: PMC2574980. (Medline) (PDF File ... Principles of RNA Compaction: Insights From the Equilibrium Folding Pathway of the P4-P6 RNA Domain in Monovalent Cations. PMID ...
http://herschlaglab.stanford.edu/publications/
*  "Small RNA Sorting in Drosophila Produces Chemically Distinct Functiona" by Michael D. Horwich
Small RNA sorting also has chemical consequences for the small RNA guide. Although siRNAs were presumed to have the signature 2 ... RNA guides that repress eukaryotic gene expression using distinct RNA Induced Silencing Complexes (RISCs). At its core, RISC is ... This allows structural information embedded in small RNA duplexes to direct small RNA loading into Ago1 and/or Ago2, resulting ... ends by the RNA 2´-O-methyltransferase DmHen1. In plants Hen1 modifies the 3´ ends all small RNAs duplexs, protecting and ...
http://escholarship.umassmed.edu/gsbs_diss/384/
*  FlyRNAi: 2016
Housden BE, Hu Y, Perrimon N. Design and Generation of Drosophila Single Guide RNA Expression Constructs. Cold Spring Harb ... RNA biology (30) RNA splicing (2) RNA-seq (1) RNAi rescue (5) screen hit follow-up (142) silkworm RNAi (2) single-cell image ... Housden BE, Hu Y, Perrimon N. Design and Generation of Drosophila Single Guide RNA Expression Constructs. Cold Spring Harb ... Finally, we show that gap repair induced by two guide RNAs more reliably converts the intervening target sequence, whereas the ...
http://flyrnai.blogspot.com.au/2016/
*  Review on RNA interference (RNAi) : gene silencing post-transcriptional process by siRNA or shRNA
... is a post-transcriptional process triggered by the introduction of double-stranded RNA (dsRNA) which leads to gene silencing in ... Once unwound, the guide strand is incorporated into the RNA Interference Specificity Complex (RISC), while the passenger strand ... HomeReview List RNA Interference - Review RNA Interference - Review. 2005. RNA interference (RNAi) is one of the most exciting ... Overexpression of exportin 5 enhances RNA interference mediated by short hairpin RNAs and microRNAs. RNA. 11(2):220-6. 11. ...
http://www.invivogen.com/review-rna-interference
*  Bioinformatic and physical characterizations of genome-scale ordered RNA structure in mammalian RNA viruses - WRAP: Warwick...
Single-stranded antisense siRNAs guide target RNA cleavage in. RNAi. Cell 110:563-574.. 28. Mason, P. W., S. V. Bezborodova, ... RNA virus entirely from cloned complementary DNAs. Proc. Natl. Acad.. Sci. USA 93:15400-15404.. 8. Cullen, B. R. 2006. Is RNA ... Long-range RNA-RNA interactions circularize the dengue. virus genome. J. Virol. 79:6631-6643.. 2. Ameres, S. L., J. Martinez, ... The RNA helicase RIG-I. has an essential function in double-stranded RNA-induced innate antiviral. responses. Nat. Immunol. 5: ...
http://wrap.warwick.ac.uk/28971/
*  Difference Between Prokaryotic and Eukaryotic RNA Polymerase l Prokaryotic vs Eukaryotic RNA Polymerase
Prokaryotic RNA polymerase produces polycistronic mRNA. Eukaryotic RNA polymerase... ... What is the difference between Prokaryotic and Eukaryotic RNA Polymerase? ... RNA polymerase can give rise to mRNA, rRNA, and tRNA. Transcription factors and transcription mediated complex are guiding the ... RNA polymerization terminates by RNA polymerase I after recognizing Sal box. RNA polymerization termination by RNA polymerase ...
http://www.differencebetween.com/difference-between-prokaryotic-and-vs-eukaryotic-rna-polymerase/
*  Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease | Genetics
This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila. ... Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease. Scott J. Gratz, Alexander M. Cummings, Jennifer N. ... Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease Message Subject (Your Name) has forwarded a page to ... Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease. Scott J. Gratz, Alexander M. Cummings, Jennifer N. ...
http://www.genetics.org/content/194/4/1029.long
*  A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity | Science
RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex. Cell 139, 945 (2009). doi:10.1016/j.cell.2009.07.040 pmid:19945378 ... RNA-guided genetic silencing systems in bacteria and archaea. Nature 482, 331 (2012). doi:10.1038/nature10886 pmid:22337052. ... Small CRISPR RNAs guide antiviral defense in prokaryotes. Science 321, 960 (2008). doi:10.1126/science.1159689 pmid:18703739. ... Structures of the RNA-guided surveillance complex from a bacterial immune system. Nature 477, 486 (2011). doi:10.1038/ ...
http://science.sciencemag.org/content/337/6096/816?ijkey=b1aeefa844a5d0e4a4718f6b6c41862be78de2f0&keytype2=tf_ipsecsha
*  Addgene: RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA. Nat ... A two plasmid system for editing bacterial genomes using an RNA-guided Cas9 nuclease. ...
https://www.addgene.org/browse/article/6312/
*  Guide RNA - Wikipedia
Guide RNAs (a.k.a. gRNA) are the RNAs that guide the insertion or deletion of uridine residues into mitochondrial mRNAs in ... The phrase "guide RNA" and "gRNA" are also used in DNA editing in the case of Cas9. Trypanosomatid protists and other ... The insertions and deletions are mediated by short guide RNA (gRNAs) which encode the editing information in the form of ... The initial editing event occurs when a gRNA forms an RNA duplex with a complementary mRNA sequence just downstream of the ...
https://en.wikipedia.org/wiki/Guide_RNA
*  Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease | Development
... a small guide RNA (sgRNA) complementary to a 20 base target DNA sequence guides an ectopically expressed, bacterially derived ... Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease Message Subject (Your Name) has sent you a ... Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease. G. Parker Flowers, Andrew T. Timberlake, ... Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease. G. Parker Flowers, Andrew T. Timberlake, ...
http://dev.biologists.org/content/141/10/2165.long
*  RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex
... Caryn R. Hale, Peng Zhao, Sara Olson, Michael O. Duff, Brenton R. ... The activity of the psiRNA-Cmr protein complex (RNA-guided RNA cleavage) bears an interesting resemblance to that of Argonaute ... Small RNA deep sequencing. Small RNA libraries were prepared using the Illumina small RNA Sample preparation kit as described ... RNAs from the CRISPR loci are hypothesized to guide the CRISPR-Cas defense response based on their potential to base pair with ...
http://pubmedcentralcanada.ca/pmcc/articles/PMC2951265/
*  Cas9 and Guide RNA with DNA [image] | EurekAlert! Science News
Upon binding with guide RNA, the two structural lobes of Cas9 reorient so that the two nucleic acid binding clefts face each ... Upon binding with guide RNA, the two structural lobes of Cas9 reorient so that the two nucleic acid binding clefts face each ... Cas9 and Guide RNA with DNA (image). DOE/Lawrence Berkeley National Laboratory ...
https://www.eurekalert.org/multimedia/pub/68343.php?from=259826
*  JCVI: RNA-guided DNA Rearrangements In Ciliates: Is the Best Genome Defence a Good Offence?
RNA-guided DNA Rearrangements In Ciliates: Is the Best Genome Defence a Good Offence? ... transcripts from the maternal genome are compared with transcripts from the zygotic genome that are processed through an RNA ... employ a genome self-defensive manoeuvre to recognise and silence such elements by homology-dependent interactions with RNA- ...
http://www.jcvi.org/cms/publications/listing/abstract/article/rna-guided-dna-rearrangements-in-ciliates-is-the-best-genome-defence-a-good-offence/
*  Guide RNA design tool
Allows an entire gene to be quickly reviewed for the best guides ... Guide RNA design tool. ...for CRISPR genome editing experiments ...
https://www.horizondiscovery.com/cell-lines/all-products/guide-rna-vector-overview/guidebook-grna-design-tool
*  PDB-6c66: CRISPR RNA-guided surveillance complex, pre-nicking - Yorodumi
Name: CRISPR RNA-guided surveillance complex with Cas3 bound in its pre-nicking state. Type: COMPLEX / Entity ID: 1,. 2,. 3,. 4 ... Title: Structure basis for RNA-guided DNA degradation by Cascade and Cas3.. Authors: Yibei Xiao / Min Luo / Adam E Dolan / ... DNA BINDING PROTEIN/DNA/RNA / CRISPR-Cas / Cascade / Cas3 / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA-RNA complex. Function ... CRISPR RNA-guided surveillance complex, pre-nicking. Components. *(CRISPR-associated protein, ...) x 4*CRISPR-associated ...
https://pdbj.org/emnavi/quick.php?id=pdb-6c66
*  Shortening guide RNA markedly improves specificity of CRISPR-Cas nucleases
... 27.01.2014 ... investigators describe how adjusting the length of the the guide RNA (gRNA) component of the synthetic enzymes called CRISPR- ... Cas RNA-guided nucleases (RGNs) can substantially reduce the occurrence of DNA mutations at sites other than the intended ... RNA »RNA segment »ZiFiT »human cell »neurodegenerative disorder ...
http://www.innovations-report.com/html/reports/life-sciences/shortening-guide-rna-markedly-improves-specificity-225341.html
*  Guide RNA vector for human INPP5A
... Description. Guide RNA vector for human INPP5A.. Please be advised this product can only be ... You're reviewing: Guide RNA vector for human INPP5A How do you rate this product? *. 1 1 star ...
https://www.horizondiscovery.com/guide-rna-vector-for-human-inpp5a
*  Emerging Technology: Concerning RNA-guided gene drives for the alteration of wild populations | eLife
RNA-guided genome editing via Cas9.. The Cas9 nuclease protein and guide RNA must first be delivered into the target cell. This ... Applications of RNA-guided gene drives. RNA-guided gene drives have the potential to merge the fields of genomic and ecological ... The guide RNA directs Cas9 to bind target DNA 'protospacer' sequences that match the 'spacer' sequence within the guide RNA. ... the RNA-guided Cas9 nuclease can be readily directed to cleave additional sequences by expressing additional guide RNAs (Figure ...
https://elifesciences.org/articles/03401
*  Addgene: Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, ...
https://www.addgene.org/browse/article/6441/
*  METHODS AND COMPOSITIONS FOR RNA-GUIDED TREATMENT OF HIV INFECTION - TEMPLE UNIVERSITY OF THE COMMONWEALTH SYSTEM OF HIGHER...
... more than one guide RNA sequence is employed, for example a first guide RNA sequence and a second guide RNA sequence, with the ... The guide RNA can be a sequence complimentary to a coding or a non-coding sequence. For example, the guide RNA can be an HIV ... Exemplary guide RNA sequences that target the U3 region of HIV-1 are shown in FIG. 13. A guide RNA sequence can comprise, for ... The guide RNA sequence can be a sense or anti-sense sequence. The guide RNA sequence generally includes a proto-spacer adjacent ...
http://www.freepatentsonline.com/y2016/0250300.html
*  Structures of Active Guide Rna Molecules and Method of Selection - Patzel, Volker
RNA-induced silencing complex) formation. The present invention also rela ... The present invention relates to methods and compositions for modulating RNA silencing efficiency by providing selective RISC ( ... calculated for isolated guide-RNA and RNAi provide compelling evidence that guide-RNA structures play a crucial role in RNA ... base-matching with guide-RNA 5′domains monitors for target specificity. Upon targeting, wobble pairings with guide-RNA 5′ ...
http://www.freepatentsonline.com/y2008/0280848.html
*  CRISPR-Cas9 in Complex with Guide RNA and DNA
The limit of a 20 bp guide is a larger restraint. As seen in the RNA-Guided DNA Targeting Section, Cas9 s end-capping ... RNA-Guided DNA Targeting Mechanism VII. Implications VIII. References I. Introduction. Clustered regularly interspaced short ... in Complex with Guide RNA and DNA. Trevor Manz '17 and Laura Duncan '17 ... A Programmable Dual-RNA Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 17: 816-821.. ...
http://biology.kenyon.edu/BMB/jsmol2015/CRISPR/indexCRISPR.html