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*  DNA-directed RNA interference - Wikipedia
DNA constructs are designed to express self-complementary double-stranded RNAs, typically short-hairpin RNAs (shRNA), that once ... Any RNA, including endogenous mRNAs or viral RNAs, can be silenced by designing constructs to express double-stranded RNA ... DNA-directed RNA interference (ddRNAi) is a gene-silencing technique that utilizes DNA constructs to activate an animal cell's ... Unlike small interfering RNA (siRNA) therapeutics that turn over within a cell and consequently only silence genes transiently ...
https://en.wikipedia.org/wiki/DNA-directed_RNA_interference
*  Natural antisense RNAs in the nervous system : Sussex Research Online
Natural antisense RNAs are endogenous molecules that are complementary to RNA transcripts of already established function. They ... Korneev, S and O'Shea, M (2005) Natural antisense RNAs in the nervous system. Reviews in the Neurosciences, 16 (3). pp. 213-222 ... Here we discuss the major known types of natural antisense RNAs in eukaryotic systems and focus on their potential roles in the ... Probably the most exciting outcome of these studies is that natural antisense RNAs are particularly prevalent in the nervous ...
http://sro.sussex.ac.uk/30312/
*  DNA-directed RNA polymerase, subunit E/RPC8 (IPR004519) | InterPro | EMBL-EBI
... are responsible for the polymerisation of ribonucleotides into a sequence complementary to the template DNA. In eukaryotes, ... Eukaryotic nuclei contain three distinct types of RNA polymerases that differ in the RNA they synthesise: *RNA polymerase I: ... RNA synthesis follows after the attachment of RNA polymerase to a specific site, the promoter, on the template DNA strand. The ... RNA polymerase III: also occurs in the nucleoplasm, synthesises the precursors of 5S ribosomal RNA, the tRNAs, and a variety of ...
http://www.ebi.ac.uk/interpro/entry/IPR004519
*  r-nastiseq - Bioconda documentation
Pairs of RNA molecules transcribed from partially or entirely complementary loci are called cis-natural antisense transcripts ( ... A promising experimental tool for profiling sense and antisense transcription is strand-specific RNA sequencing (ssRNA-seq). To ...
http://bioconda.github.io/recipes/r-nastiseq/README.html
*  Bent helix formation between RNA hairpins with complementary loops | Science
Bent helix formation between RNA hairpins with complementary loops Message Subject. (Your Name) has forwarded a page to you ... The initial interaction between the ColE1 plasmid specific transcripts RNA I and RNA II, which function as antisense regulators ... comprises a transient complex between complementary loops found within the RNA secondary structures. Multidimensional ... spectroscopy was used to characterize complexes formed between model RNA hairpins having seven nucleotide complementary loops. ...
http://science.sciencemag.org/content/268/5216/1448
*  miRcore: Understanding BLAST and Reverse Complementary RNA, and Discussing miRcore's Future Leadership
The 5' end of the RNA will match up with the 3' end of the DNA, and vice versa. The RNA goes in the reverse direction compared ... For the example below, the reverse complementary RNA for the positive strand, read from the 5' end, would be CAUCCU . . . the ... Home » Blog » 2017-18 School Year » Understanding BLAST and Reverse Complementary RNA, and Discussing miRcore's Future ... However, another element of complexity is that RNA is processed by having parts removed, i.e. introns. So not all the RNA that ...
http://www.mircore.org/3242018-blog-post-by-eleanor-lin/
*  Recovery of Infectious Ebola Virus from Complementary DNA: RNA Editing of the GP Gene and Viral Cytotoxicity | Science
Recovery of Infectious Ebola Virus from Complementary DNA: RNA Editing of the GP Gene and Viral Cytotoxicity ... Recovery of Infectious Ebola Virus from Complementary DNA: RNA Editing of the GP Gene and Viral Cytotoxicity ... Recovery of Infectious Ebola Virus from Complementary DNA: RNA Editing of the GP Gene and Viral Cytotoxicity ... Recovery of Infectious Ebola Virus from Complementary DNA: RNA Editing of the GP Gene and Viral Cytotoxicity ...
http://science.sciencemag.org/content/291/5510/1965
*  Rna Base Pairing 4 Basics Bases Complementary Rules For - NapTimeisforDrinking
rna base pairing 4 basics bases complementary rules for.. rna intramolecular base pairing drives phase transitions the ... dna to rna base pairing example double stranded acid forms by between chapter 12 and,dna rna base pairing rules complementary ... dna rna base pairing rules of complementary structure and dynamics a perspective,hydrogen bonding in base pairs does rna have ... rna base pairing worksheet complementary dna to nitrogen rules,dna to rna base pairing example cytoplasm use the rules choose ...
http://naptimeisfordrinking.me/rna-base-pairing/rna-base-pairing-4-basics-bases-complementary-rules-for/
*  Plus it
complementary RNA. DMSO. dimethylsulfoxide. GluR. glutamate receptor subunit. GPCR. G protein-coupled receptor. HRP. ... The T7 in vitro transcription kit was used for NR1 and NR2A complementary RNAs (cRNAs), and the SP6 kit was used for NR2B cRNAs ... Preparation of Complementary RNA.. Plasmids were linearized with appropriate restriction enzymes prior to in vitro ... transcription using mMESSAGE mMACHINE High Yield Capped RNA Transcription kits (Ambion, Inc., Austin, TX). Plasmids containing ...
http://molpharm.aspetjournals.org/content/84/2/261
*  Subcellular distribution of signal recognition particle and 7SL-RNA determined with polypeptide-specific antibodies and...
... distribution of signal recognition particle and 7SL-RNA determined with polypeptide-specific antibodies and complementary DNA ... Thus it is likely that the major-if not the only-cellular function of 7SL-RNA is as a part of SRP. ... Furthermore, it is shown that greater than 75% of the total cellular 7SL-RNA is associated with SRP polypeptide in these ... Walter P, Blobel G. Subcellular distribution of signal recognition particle and 7SL-RNA determined with polypeptide-specific ...
http://walterlab.ucsf.edu/publication/562/subcellular-distribution-of-signal-recognition-particle-and-7sl-rna-determined-with-polypeptide-specific-antibodies-and-complementary-dna-probe/
*  piRNAs initiate an epigenetic memory of non-self RNA in the C. elegans germline
21U-RNAs complementary to gfp are correlated with 22G biogenesis. Our findings suggest that transgene silencing is initiated by ... EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans. Curr ... 40 nt window around the site of 21U-RNA complementarity on the target RNA. We found 8 regions on gfp where 22G-RNAs were ... Piwi-interacting (pi) RNAs (21U-RNAs in C. elegans) are genomically encoded and appear to be expressed as Pol II transcripts ...
http://pubmedcentralcanada.ca/pmcc/articles/PMC3597741/?lang=en-ca
*  Final Exam- Lecture Chap. 10 Flashcards by LILIAN COLLADA | Brainscape
manufacture of a strand of RNA complementary to a strand of DNA ... The transfer of information from DNA into an RNA molecule is ... RNA contains the nitrogenous base ________ instead of ________, which is found only in DNA ... What type of chemical bond joins the bases of complementary DNA strands? ...
https://www.brainscape.com/flashcards/final-exam-lecture-chap-10-5517710/packs/8026850
*  Virus | Encyclopedia.com
Although viruses share several features with living organisms, such as the presence of genetic material (DNA or RNA), they are ... An RNA or DNA molecule with the reverse complementary sequence to mRNA is a negative-sense strand. A few viruses have been ... RNA-dependent RNA polymerase) and associated cofactors. Single-stranded negative-sense RNA viruses of animals must also carry a ... Complementary RNA. A U U G G G C U C. negative. ... since this RNA-to-RNA enzymatic activity is typically lacking ...
https://www.encyclopedia.com/plants-and-animals/microbes-algae-and-fungi/moneran-and-protistan/virus
*  Abbreviations and Conventions | Journal of Clinical Microbiology
cDNA (complementary DNA). *RNA (ribonucleic acid). *cRNA (complementary RNA). *RNase (ribonuclease). *DNase (deoxyribonuclease) ... tRNA (transfer RNA). *AMP, ADP, ATP, dAMP, ddATP, and GTP, etc. (for the respective 5′ phosphates of adenosine and other ...
https://jcm.asm.org/content/abbreviations-and-conventions
*  Programmable RNA recognition using a CRISPR-associated Argonaute | PNAS
S1 and S5A) and can bind complementary RNA substrates more than 300 times more efficiently than noncomplementary RNAs with a ... ON-target RNA), or an excess of total RNA purified from HEK293T cells (OFF-target RNA) (SI Appendix, Fig. S2A). Notably, in the ... For example, some pAgos can use either DNA or RNA guides, and can target either DNA or RNA substrates (12⇓⇓-15). Differing from ... However, binding of fully complementary target RNAs to MpAgo RNPs with gRNAs that have longer 3′ extensions can destabilize the ...
https://www.pnas.org/content/115/13/3368
*  HBG1 - Wikipedia
1977). "Human beta-globin messenger RNA. I. Nucleotide sequences derived from complementary RNA". J. Biol. Chem. 252 (14): 5019 ... Proudfoot NJ, Brownlee GG (1976). "3' non-coding region sequences in eukaryotic messenger RNA". Nature. 263 (5574): 211-4. doi: ...
https://en.wikipedia.org/wiki/HBG1
*  Transcriptomic profiling of bovine IVF embryos revealed candidate genes and pathways involved in early embryonic development |...
Using microarrays, genome-wide RNA expression was profiled and compared for in vitro fertilization (IVF) - derived blastocysts ... Linear amplification and labeling of complementary RNA (cRNA). Due to the limited amount of RNA present in embryos, a two-round ... RNA extracted/amplified from pools of embryos and RNA used for real time RT-PCR. Sources of RNA used for the real time RT-PCR ... Pooling of embryos and amplification of RNA in this study were necessitated by scarcity of RNA present in embryos [5, 6]. These ...
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-11-23
*  Plus it
complementary RNA. FRET. Förster resonance energy transfer. FLIM. fluorescence lifetime imaging. YPD. yeast peptone dextrose. ... we included complementary RNAs (cRNAs) for each of the VAMP constructs in a 1:4 KAT1:VAMP ratio, and expression was verified by ... These measurements were then repeated with the complementary VAMP721 constructs VAMP721∆C-GFP, mCherry-VAMP721∆C-GFP, and ... that the R-SNARE plays a key role in coordinating vesicle traffic and ion transport in a manner complementary to SYP121. ...
http://www.plantphysiol.org/content/173/1/536
*  Chromosome biorientation and APC activity remain uncoupled in oocytes with reduced volume | JCB
complementary RNA. H2B. Histone 2B. MI. meiosis I. NEB. nuclear envelope breakdown. PBE. polar body extrusion. SAC. spindle ... To verify that the oocytes' reduced volume did not affect their ability to form a spindle, we microinjected complementary RNA ( ... RNA polymerase kit (Ambion) (Lane and Jones, 2014). cRNA was suspended in nuclease-free water, and its concentration was ...
http://jcb.rupress.org/content/early/2017/10/03/jcb.201606134.full
*  The netrin receptor UNC5B promotes angiogenesis in specific vascular beds | Development
Placentas from E12.5 embryos were hemisected and probed with antisense RNA complementary to Unc5b mRNA. (A) Unc5b+/flox;+/Tg( ... were probed with RNA complementary to Unc5b mRNA. As shown in Fig. 3A,B, binding of this probe was restricted to the labyrinth ... Antisense digoxigenin-labeled riboprobe was generated from mouse Unc5b DNA using a DIG RNA Labeling Kit (Roche). In situ ...
http://dev.biologists.org/content/135/4/659.long
*  Plus it
RNA and Complementary DNA Preparation for T-Cell Repertoire Analysis.. RNA was extracted using guanidium hydrochloride- ... First-strand cDNA synthesis was performed using oligo(dT) as a primer for reverse transcription of 1 μg of total RNA using ...
http://cancerres.aacrjournals.org/content/64/21/8068
*  Abbreviations and Conventions | Microbiology and Molecular Biology Reviews
cDNA (complementary DNA). *RNA (ribonucleic acid). *cRNA (complementary RNA). *RNase (ribonuclease). *DNase (deoxyribonuclease) ... tRNA (transfer RNA). *AMP, ADP, ATP, dAMP, ddATP, and GTP, etc. (for the respective 5′ phosphates of adenosine and other ...
https://mmbr.asm.org/content/abbreviations-and-conventions
*  Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APCCdh1 | JCB
Oocytes were coinjected with a 70-kD rhodamine-dextran to provide an accurate timing of GVBD and with complementary RNA (cRNA) ... Abbreviations used in this paper: APC, anaphase-promoting complex; cRNA, complementary RNA; CSF, cytostatic factor; Emi1, early ... and transcribed to synthesize 35S-labeled RNA probes. Hybridization mixtures with antisense and sense RNA probes were added to ...
http://jcb.rupress.org/content/176/1/65.long
*  Patent US8153422 - Dopaminergic neurons differentiated from embryonic cells for treating ... - Google Patents
... overexpressing in the pluripotent cells small interfering RNA complementary to the RNA encoding the signaling pathway component ... synthetic RNA, recombinantly produced RNA) as well as altered RNA that differs from naturally occurring RNA by the addition, ... dsRNAs are typically used to mediate RNA interference. "RNA interference (RNAi)" describes the use of double-stranded RNA ( ... wherein said Smad4 is inhibited by overexpressing small interfering RNA complementary to the mRNA encoding Smad4 in said ...
http://www.google.com/patents/US8153422
*  Patent US6811977 - Rapid, quantitative method for the mass spectrometric analysis of nucleic ... - Google Patents
The presence and absolute or relative amount of a given mass tag will reflect the amount of the target complementary RNA ... RNA preparation, or other biological sample containing RNA or a nucleotide representation of the RNA such as cDNA) by the ... Thus, RNA extraction must be from sizable amounts of tissue, cell or embryo cultures, or that the initial RNA sample must be ... RNAs, complementary to a detector oligonucleotide species. In certain embodiments of the invention, separation is not necessary ...
http://www.google.com/patents/US6811977?dq=6,125,447