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*  Microbiology Society Journals | Individual influenza A virus mRNAs show differential dependence on cellular NXF1/TAP for their...
... an inhibitor of RNA polymerase II phosphorylation previously shown to inhibit nuclear export of cellular mRNA as well as ... The influenza A virus RNA-dependent RNA polymerase produces capped and polyadenylated mRNAs in the nucleus of infected cells ... The influenza A virus RNA-dependent RNA polymerase produces capped and polyadenylated mRNAs in the nucleus of infected cells ... Association of the influenza A virus RNA-dependent RNA polymerase with cellular RNA polymerase II. J Virol 79, 5812-5818.[ ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.018564-0
*  RNA cap 4 binding QuickView - Correlation Engine
... cap 4): 7-methylguanosine-ppp-N6, N6, 2'-O-trimethyladenosine-p-2'-O-methyladenosine-p-2'-O-me… ... Interacting selectively and non-covalently with a hypermethylated cap structure consisting of 7-methylguanosine (m(7)G) ...
http://www.nextbio.com/b/search/ov/RNA%20cap%204%20binding?id=30765&type=biogroup
*  Studies of RNA capping enzymes - AFMB UMR 7257
... end of their RNAs. This structure is essential for the translation of mRNA into proteins. In addition, (...) ... Many viruses add a cap structure at the 5' ... Studies of RNA capping enzymes. Studies of RNA capping enzymes ... Mechanism of cap structure synthesis. Some references. The viral RNA capping machinery as a target for antiviral drugs. (2012) ... In addition, the cap structure lures the detection of foreign RNA by innate immunity sensors. The addition of the cap structure ...
http://www.afmb.univ-mrs.fr/studies-of-rna-capping-enzymes
*  RNA Cap Methyltransferase Activity Assay -BIO-PROTOCOL
G-capped RNA substrate and S-adenosylmethionine (SAM) to produce RNAs with N7-methylated caps. The extent of cap methylation is ... The protocol described here includes additional steps for generating the [32P]G-capped RNA substrate and for preparing nuclear ... Here we provide a detailed protocol for the biochemical analysis of RNA cap methyltransferase activity of biological samples. ... This assay is also applicable to analyzing the cap methyltransferase activity of other biological samples, including ...
https://bio-protocol.org/e2767
*  CMTr1-DXH15 interactions in RNA capping | Philosophical Transactions of the Royal Society B: Biological Sciences
... characteristic for RNA polymerase II-transcribed RNAs, plays important roles in RNA metabolism. In humans, RNA cap formation ... 2014 RNA methyltransferases involved in 5′ cap biosynthesis. RNA Biol. 11, 1597-1607. (doi:10.1080/15476286.2015.1004955). ... Our findings suggest a new mechanism underlying the regulatory role of DHX15 in the RNA capping process. RNAs with highly ... Thus far, RNA methylation has been found to influence RNA structure. In particular, m6A alters local RNA structure and acts as ...
http://rstb.royalsocietypublishing.org/content/373/1762/20180161
*  Kits and methods for generating 5′ capped RNA - CellScript, LLC
... capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. In particular, ... capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. In particular, the ... present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such ...
http://www.freepatentsonline.com/8846348.html
*  Rapidly Synthesize Large Amounts of Capped RNA | Thermo Fisher Scientific - ZA
Each reaction synthesizes 10-50 times the yield of capped RNA obtained with conventional in vitro transcription reactions. ... High Yield Capped RNA Transcription Kits use Ambion's patented high yield transcription technology. ... We also offer the mMESSAGE mMACHINE T7 Ultra Kit for the generation of RNA transcripts capped with Anti Reverse Cap Analog ( ... Capped RNA that Gives Higher Protein Yields mMESSAGE mMACHINE T7 Ultra › * High Yield Transcription for Every Application , ...
https://www.thermofisher.com/za/en/home/references/ambion-tech-support/large-scale-transcription/tech-notes/rapidly-synthesize-large-amounts-of-capped-rna.html
*  RNA trimethylguanosine cap binding QuickView - Correlation Engine
... end of some RNA molecules. Such trimethylated cap structures, generally produced by posttranscriptional modification of a 7- ...
http://www.nextbio.com/b/search/ov/RNA%20trimethylguanosine%20cap%20binding?id=30764&type=biogroup
*  RNA Capping by Transcription Initiation with Non-canonical Initiating Nucleotides (NCINs): Determination of Relative...
... for transcription initiation by bacterial and eukaryotic cellular RNA polymerases (RNAPs) and that the efficiency of the ... Keywords: RNA polymerase, Transcription, Non-canonical initiating nucleotide (NCIN), RNA capping, ab initio RNA capping, NAD+, ... Ebright, R. H. (2000). RNA polymerase: structural similarities between bacterial RNA polymerase and eukaryotic RNA polymerase ... followed by quantifying NCIN-initiated RNA and total RNA, followed by plotting observed ratios of NCIN-initiated RNA to total ...
https://bio-protocol.org/p.aspx?id=e2336
*  US Patent Application for Synthesis and Use of Anti-Reverse Phosphorothioate Analogs of the Messenger RNA Cap Patent...
New RNA cap analogs are disclosed containing one or more phosphorothioates groups. The analogs also contain modifications at ... "capped." In addition, there are some other forms of RNA that are also capped, for instance small nuclear RNAs (snRNAs). The cap ... 5′ Degradation of mRNAs Capped with Phosphorothioate Cap Analogs. Because short RNAs capped with m27,2′-OGppspG (D2) were ... A. Pasquinelli et al., "Reverse 5′ caps in RNAs made in vitro by phage RNA polymerases," RNA, vol. 1, pp. 957-967 (1995), ...
https://patents.justia.com/patent/20100233757
*  Robotic Packaging System Packs Filler Caps - RNA Automation
... with a bespoke robot gripper to suit the cap and the corrugated interleafs. ... RNA develped an automated robotic packaging system for packaging filler caps, ... Written by: RNA Automation. RNA developed an automated robotic packaging system for packaging 2 different types of filler caps ... RNA developed an automated robotic packaging system for packaging 2 different types of filler caps. The system utilises a ...
https://www.rnaautomation.com/blog/robotic-packaging-system-packs-filler-caps/
*  A yeast-like mRNA capping apparatus in Plasmodium falciparum | PNAS
... the diphosphate end is capped with GMP by GTP:RNA guanylyltransferase, and the GpppN cap is methylated by AdoMet:RNA (guanine- ... G) RNA cap formation. The RNA reaction product was analyzed by TLC before and after digestion with nuclease P1 and alkaline ... Trypanosoma brucei Encodes a Bifunctional Capping Enzyme Essential for Cap 4 Formation on the Spliced Leader RNA ... 200-aa N-terminal RNA triphosphatase domain present in metazoan and higher plant capping enzymes (4). Metazoan RNA ...
http://www.pnas.org/content/98/6/3050.full
*  A Single Amino Acid Mutation in the PA Subunit of the Influenza Virus RNA Polymerase Inhibits Endonucleolytic Cleavage of...
To induce the capped RNA-specific endonuclease activity of the polymerase, required for generating capped RNA primers for ... Partially purified wild-type RNA polymerase, cross-linked to capped RNA radiolabeled in its cap structure, produced two ... In the capped RNA-primed transcription assays, the ApG was replaced with an 11-nt 32P-labeled capped RNA (about 5,000 cpm; see ... a capped RNA can be cross-linked to both PB1 and PB2, and amino acid sequences involved in binding and cleaving capped RNA have ...
https://jvi.asm.org/content/76/18/8989
*  Cofactor Genomics Launches Pinnacle - a CAP-CLIA Certified RNA-Based Oncology Assay | RNA-Seq Blog
Cofactor became one of the first laboratories to receive CAP-accreditation for RNA-seq. Pinnacle is built on the foundation of ... Recognizing RNA's ability to identify relevant targets in cancer drug development, as well as more accurately predict the ... Pinnacle is offered as a laboratory-developed test exclusively through Cofactor's CAP-accredited laboratories. Pinnacle will be ... A Unique Cancer Diagnostic Tool that Harnesses the Power of RNA' on Tuesday, February 21 at 11:45 am - 12:15 pm in the ...
http://www.rna-seqblog.com/cofactor-genomics-launches-pinnacle-a-cap-clia-certified-rna-based-oncology-assay/
*  A Protective Cap for Bacterial RNA « Quantum Related Diseases Research Group (QRDRG)
A Protective Cap for Bacterial RNA. For the first time, researchers from Heidelberg University have deciphered the function of ... These molecular helpers remove the protective cap at the start of ribonucleic acid (RNA) molecules. This decapping destabilises ... Dr Andres Jäschke and his bioorganic chemistry working group now revealed these mechanisms in bacterial RNA. (Mehr in: ... August 3rd, 2016 , Tags: cell, CT, researcher, RNA , Category: Medizin-Biologie-Physik ...
http://diseasesresearchgroup.xonl.de/2016/08/03/a-protective-cap-for-bacterial-rna/
*  Rational design of novel N-alkyl-N capped biostable RNA nanostructures for efficient long-term inhibition of gene expression
RNA. Molecular dynamics. DNA--Analysis. RNA. Breast cancer cells. RNA interference (RNAi). Oligonucleotide sequences. ADN. ... Rational design of novel N-alkyl-N capped biostable RNA nanostructures for efficient long-term inhibition of gene expression ... while showing interference RNA activity. The in silico designed structure consists of a 24-29 bp duplex RNA region linked on ... Computational techniques have been used to design a novel class of RNA architecture with expected improved resistance to ...
http://www.recercat.cat/handle/2072/281749
*  Cap Binding Complex in RNA Processing and Transport - Tracy Zimmermann
The goal of the proposed research is to understand in detail the role of the cap binding complex (CBC) in mRNA processing and ... Cap Binding Complex in RNA Processing and Transport Zimmermann, Tracy K. Dana-Farber Cancer Institute, Boston, MA, United ... Cap Binding Complex in RNA Processing and Transport. Zimmermann, Tracy K. / Dana-Farber Cancer Institute. $37,516. ... Cap Binding Complex in RNA Processing and Transport. Zimmermann, Tracy K. / Dana-Farber Cancer Institute. ...
http://grantome.com/grant/NIH/F32-GM019487-01
*  Synergy between Escherichia coli CAP protein and RNA polymerase in the lac promoter open complex.
CAP protein and RNA polymerase, separated on non-denaturing polyacrylamide gels and footprinted in the gel ...
https://www.wizdom.ai/publication/10.1016/0022-2836
*  LSM1 Gene - GeneCards | LSM1 Protein | LSM1 Antibody
Gene Ontology (GO) annotations related to this gene include RNA cap binding. ... RNA splicing, via transesterification reactions. TAS. 10369684. GO:0000956. nuclear-transcribed mRNA catabolic process. IEA. -- ... Inhibitory RNA Products. * Origene shrna, sirna, and RNAi products in human, mouse, rat for LSM1 ... Inhibitory RNA Products. * Origene shrna, sirna, and RNAi products in human, mouse, rat for LSM1 ...
https://www.genecards.org/cgi-bin/carddisp.pl?gene=LSM1
*  EIF4A1 - Eukaryotic initiation factor 4A-I - Homo sapiens (Human) - EIF4A1 gene & protein
In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is ... ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding ... RNA cap binding Source: UniProtKB ,p>Traceable Author Statement,/p> ,p>Used for information from review articles where the ... ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding ...
http://www.uniprot.org/uniprot/P60842
*  SNUPN - Snurportin-1 - Homo sapiens (Human) - SNUPN gene & protein
Binds specifically to the terminal m3G-cap U snRNAs. ... Involved in the trimethylguanosine (m3G)-cap-dependent nuclear ... RNA cap binding Source: ProtInc ,p>Traceable Author Statement,/p> ,p>Used for information from review articles where the ... Involved in the trimethylguanosine (m3G)-cap-dependent nuclear import of U snRNPs. Binds specifically to the terminal m3G-cap U ... "Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure.". Huber J., Cronshagen U., Kadokura M. ...
https://www.uniprot.org/uniprot/O95149
*  A proposed model for the stage-specific mRNA degradatio | Open-i
RNase-H-RNA blot analysis revealed that a major part of the rapid promastigote-specific degradation of the amastin mRNA is not ... RNase-H-RNA blot analysis revealed that a major part of the rapid promastigote-specific degradation of the amastin mRNA is not ... This is in contrast to the amastin mRNA in amastigotes and to reporter RNAs lacking the URE, which, in common with most ... This is in contrast to the amastin mRNA in amastigotes and to reporter RNAs lacking the URE, which, in common with most ...
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2275140_gkn019f7&req=4
*  Capping enzyme - Wikipedia
When this complex of RNA polymerase II and the capping enzymes is achieved, the capping enzymes are able to add the cap to the ... cap is therefore specific to RNAs synthesized by this polymerase rather than those synthesized by RNA polymerase I or RNA ... A capping enzyme (CE) is an enzyme that catalyzes the attachment of the 5' cap to messenger RNA molecules that are in the ... Last, RNA methyltransferase transfers a methyl group to the guanosine cap to yield 7-methylguanosine cap that is attached to ...
https://en.wikipedia.org/wiki/Capping_enzyme
*  Optimize Your TNT® Reticulocyte Lysate Systems Reaction
Capped RNA is not Required. For in vitro translation kits, capping of the RNA is not usually required for protein synthesis( ... capping of the RNA may be beneficial. The effect of capping on translation efficiency will be template-dependent. The TnT® ... which does not need to be capped for translation. To determine if a 5´ cap is required for efficient translation of your RNA of ... Svitkin, Y.V. et al. (1996) General RNA binding proteins render translation cap dependent. EMBO J. 15, 7147-55. ...
https://www.promega.com/resources/pubhub/enotes/optimize-your-tnt-reticulocyte-lysate-systems-reaction/
*  Anti-eIF4E antibody [Y449] Recombinant (ab33768) | Abcam
Messanger RNA Cap Binding Protein eIF 4E antibody. *MGC111573 antibody. *mRNA cap binding protein antibody ... Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1- ... Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. ... Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and ...
https://www.abcam.com/eif4e-antibody-y449-ab33768.html