These short RNAs participate in a diverse array of cellular processes including gene regulation, chromatin dynamics and genome ... RNAs, and the centromere repeat associated short interacting (crasi) RNAs in the tammar. We examined each of these small RNA ... we have characterized the major classes of small RNAs, micro (mi) RNAs, piwi interacting (pi) ... These small RNAs are derived largely from centromere-enriched retroelements, including a novel SINE. This study encompasses the ...
... end Processing Machinery Wu, Yixuan 2018 Theses BiologyBiochemistryCatalytic RNA ... You searched for: Subject Catalytic RNA ✖ Remove constraint Subject: Catalytic RNA Degree Level Doctoral ✖ Remove constraint ... 1. Structural Studies of the Integrator Complex -- pre-UsnRNA 3- ...
... end processing. RNA pull-down assay showed that Star-PAP could bind to 3′region of miR-449a. In line with these results, ... end processing and expression of a specific set of mRNAs and microRNAs. This process is very critical to stability and post- ... end processing way in breast cancer cells (Fig. 2E). Furthermore, the proposed Star-PAP-miR-449a/34a-TPD52 axis is involved in ... end processing by Star-PAP is still at a nascent stage. Given that TPD52 is closely associated with tumor progression, our ...
... end processing and transcription initiation. Mapendano, C. K., Lykke-Andersen, S., Kjems, J., Bertrand, E. & Jensen, T. H., ... Lloret-Llinares, M., Mapendano, C. K., Martlev, L. H., Lykke-Andersen, S. & Jensen, T. H., 2016, I: RNA Biology. 13, 1, s. 6-14 ... An ending is a new beginning: transcription termination supports re-initiation. Lykke-Andersen, S., Mapendano, C. K. & Jensen, ... RNA exosome depletion reveals transcription upstream of active human promoters. Preker, P., Nielsen, J., Kammler, S., Lykke- ...
... is essential for processing and maturation of mammalian 5.8S and 28S ribosomal RNAs. Applying a mass spectrometric analysis, we ... end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex. ... This recruitment is RNA-dependent and occurs independently of the PeBoW-complex. Interestingly, knockdown of DDX27, but not of ... end formation of 47S rRNA independently of the PeBoW-complex. ...
To this end, we raised an antiserum that selectively recognizes the 5-HT1D receptor. Although our studies do not answer the ... Corneal tissue was processed as free-floating sections.. Ligated sciatic nerve. Male Sprague Dawley rats (300-400 gm) were ... Pierce PA, Xie GX, Meuser T, Peroutka SJ ( 1997) 5-Hydroxytryptamine receptor subtype messenger RNAs in human dorsal root ... Dural tissue was collected after perfusion and processed as a whole mount. To collect corneas, the eyes were superfused with ...
Two reporter-expressing ZIKVs were then generated by transfection of ZIKV-susceptible BHK-21 cells with infectious RNAs derived ... 10.8 kb plus-strand RNA genome that can cause neurological disease. To facilitate the identification of potential antivirals, ... end for in vitro run-off transcription. Following RNA transfection, the infectious ZIKV RNAs, like the viral genomic RNA, ... long-distance RNA-RNA interactions [1,26]. The ORF encodes a large polyprotein that is processed by both host cell- and virus- ...
... expressed in the nucleus and associated with the C-terminal domain of RNA polymerase (RNA Pol II). The work of the scientists ... These modifications include the addition of a cap at the 5 end and polyadenosines tail at the 3 end, as well as the addition ... In living organisms, gene expression is a complex process that results in the production of proteins from the genome in a time ... RNA 2023. Pandey RR, Delfino E, Homolka D, Roithova A, Chen KM, Li L, Franco G, Vågbø CB, Taillebourg E, Fauvarque MO and ...
... end processing of long noncoding RNA initiates construction of nuclear paraspeckles. EMBO J 31:4020-4034. ... Paraspeckles trap RNA, RNA-binding proteins, and transcription factors to suppress the transition of RNA into the cytoplasm ( ... By using FITC RNA Labeling Mix and ScriptMAX Thermo T7 Transcription Kit, FITC-labeled antisense RNA probe was transcribed ... A) Schematic representation of NEAT1_2 RNA and the target region of the RNA probe. (B) FISH assay for NEAT1_2 and ...
Strong evidence indicates that transcription elongation by RNA polymerase II (pol II) is a highly regulated process. Here we ... end processing. The conservation of Paf1 complex function in higher eukaryotes has been confirmed in human cells, Drosophila ... The complex regulates histone H2B ubiquitination, histone H3 methylation, RNA polymerase II carboxy-terminal domain (CTD) Ser2 ... Crystal structure of RNA polymerase-associated protein RTF1 homolog Plus-3 domain. ...
The recruitment of this machinery results in the production of viroid-derived small RNAs (vd-sRNAs) that mediate RNA ... Members of the Pospiviroidae family replicate in the nucleus through double-stranded RNA intermediates, attracting the host RNA ... Viroids are pathogenic circular, non-coding, single-stranded RNA molecules. ... At least in plants, DNA regions that become de novo methylated can be defined by homologous RNA molecules in a process termed ...
... that act directly on the CMS conferring mitochondrial transcripts by binding them specifically and promoting processing events ... that act directly on the CMS conferring mitochondrial transcripts by binding them specifically and promoting processing events ... Jonietz, C., Forner, J., Hölzle, A., Thuss, S., and Binder, S. (2010). RNA PROCESSING FACTOR2 is required for 5′ end processing ... suggesting that they may be involved in RNA stabilization, processing or transport. Some class IV GRPs also have an RNA- ...
Linking RNA Processing and Function. Run-Wen Yao, Chu-Xiao Liu, and Ling-Ling Chen. Attenuation of Eukaryotic Protein-Coding ... End for Tailing and Trimming during Target-Directed microRNA Degradation. Paulina Pawlica, Jessica Sheu-Gruttadauria, Ian J. ... Long Noncoding RNAs; RNA & Development; Membrane-Less Organelles; Phase Separation; RNA-Based Diseases; and Novel RNA Functions ... The topics covered at the meeting included: RNA-Based Structures; RNA Modifications; Nuclear Localization of RNA; Quality ...
Linking RNA Processing and Function. Run-Wen Yao, Chu-Xiao Liu, and Ling-Ling Chen. Attenuation of Eukaryotic Protein-Coding ... End for Tailing and Trimming during Target-Directed microRNA Degradation. Paulina Pawlica, Jessica Sheu-Gruttadauria, Ian J. ... Long Noncoding RNAs; RNA & Development; Membrane-Less Organelles; Phase Separation; RNA-Based Diseases; and Novel RNA Functions ... The topics covered at the meeting included: RNA-Based Structures; RNA Modifications; Nuclear Localization of RNA; Quality ...
Linking RNA Processing and Function. Run-Wen Yao, Chu-Xiao Liu, and Ling-Ling Chen. Attenuation of Eukaryotic Protein-Coding ... End for Tailing and Trimming during Target-Directed microRNA Degradation. Paulina Pawlica, Jessica Sheu-Gruttadauria, Ian J. ... Long Noncoding RNAs; RNA & Development; Membrane-Less Organelles; Phase Separation; RNA-Based Diseases; and Novel RNA Functions ... The topics covered at the meeting included: RNA-Based Structures; RNA Modifications; Nuclear Localization of RNA; Quality ...
Most cellular processes are regulated by RNA-binding proteins (RBPs). These RBPs usually use defined binding sites to recognize ... end is protected from premature degradation by synthesis of a poly(A) tail. These processes are catalyzed by four multi-protein ... In this PhD thesis I studied how RNA processing is driven from RBP binding by analyzing iCLIP-derived sequencing datasets. ... Using deep RNA-Sequencing (RNA-Seq), I investigated transcriptome changes of three human cell lines from lung, cervical and ...
... end RNA processing. Our attempts to examine the fate of polymerase II itself were inconclusive, because only trace ... end RNA processing. Our attempts to examine the fate of polymerase II itself were inconclusive, because only trace ... end RNA processing. Our attempts to examine the fate of polymerase II itself were inconclusive, because only trace ... end RNA processing. Our attempts to examine the fate of polymerase II itself were inconclusive, because only trace ...
total RNA. Extraction protocol. Single-cell suspensions were processed through Drop-Seq to generate single-cell cDNA libraries ... end of the cDNA prepared for sequencing using a modified Nextera XT protocol.. ... Data processing. Base calls performed with bcl2fastq2.15. Read 2 was aligned with Star v2.4.0. Each aligned read was tagged ... Microbeads were counted, and amplified by PCR, and the 3 ... Processed data provided as supplementary file. Processed data ...
... end processing complex that led to the discovery of several novel proteins that connect mRNA processing with various nuclear ... Stefania Policicchio Post Doc 3D Chromatin Conformation and RNA Genomics Ilaria Rapone Support Administrative Assistant D3 ... Julien Jean-Claude Michel Ouvrard Post Doc 3D Chromatin Conformation and RNA Genomics ... Morgana Dalla Palma PhD Student Fellow 3D Chromatin Conformation and RNA Genomics ...
The output of protein-coding genes shifts to circular RNAs when the pre-mRNA processing machinery is limiting. Mol Cell 68: 940 ... ends and theoretically can circularize. However, every exon does not circularize, and, in some cases, multiple exons are ... producing circular noncoding RNAs rather than linear messenger RNAs. For some genes, the abundance of the circular RNA exceeds ... Upon inhibiting RNA polymerase II termination, circular RNA levels were similarly increased due to readthrough transcripts ...
... trimming one or both of the ends of the primary transcript to the mature RNA length; removing ... There are three main types of RNA processing events: ... Messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA ( ... removing internal RNA sequences by a process called RNA splicing; and modifying RNA nucleotides either at the ends of an RNA or ... There are three types of RNA involved in the translation process: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA ...
To this end, we develop models to explain various properties of RNAs, such as the usage of specific processing sites or the ... end processing at the coding-region proximal site leads to a short 3UTR isoform, while the processing at the distal site ... The production, processing and degradation of RNAs is modulated by various protein complexes that recognize short sequence ... This enabled us to identify novel regulators of RNA processing. We are currently extending KAPAC to work with a more general ...
RNA uses uracil (U) instead of thymine (T) - DNA and RNA molecules have two chemically distinct ends (called 5 and 3) - DNA ... small RNAs (e.g., snoRNA play key roles in mRNA processing) - DNA serves mainly for information storage - proteins are often ... messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA) - structure and function of proteins - function of DNA and RNA ... proteins and RNA and started discussing the basic processes of gene expression (transcription and translation). Important ...
... has a great contribution in the RNA replication process. The most crucial NS proteins are the viral helicase and the viral RNA- ... ends. The translated polyprotein consists of three structural [capsid (C), membrane (M) and envelope (E)] and seven non- ... Study of the langat virus RNA-dependent RNA polymerase through homology modeling. EMBnet J. 26(e944)2021.PubMed/NCBI View ... An updated evolutionary study of Flaviviridae NS3 helicase and NS5 RNA-dependent RNA polymerase reveals novel invariable motifs ...
The RNA FRABASE 2.0 search engine algorithms operate on the database of the RNA sequences and the new library of RNA secondary ... Our intention is that this advanced version of the RNA FRABASE will be of interest to all researchers working in the RNA field. ... This search is based upon the user-defined RNA secondary structure pattern. In this paper, we present and discuss RNA FRABASE ... RNA FRABASE 2.0 has stored information on 1565 PDB-deposited RNA structures, including all NMR models. ...
... end processing of long noncoding RNA initiates construction of nuclear paraspeckles. EMBO J 2012;31:4020-34. [Crossref] [PubMed ... A recent study by Knutsen et al., has assessed the expression of NEAT1 isoforms in breast cancer (10). Using RNA-FISH and RNA- ... Aims and Scope Non-coding RNA Investigation (Non-coding RNA Investig; NCRI; online ISSN: 2522-6673) is an international, open ... RNA 2017;23:872-81. [Crossref] [PubMed]. *Knutsen E, Lellahi SM, Aure MR, et al. The expression of the long NEAT1_2 isoform is ...
Metabolism of RNA (Homo sapiens) * * tRNA processing (Homo sapiens) * tRNA processing in the mitochondrion (Homo sapiens) * ... end of pre-tRNA (Homo sapiens) * CTP [mitochondrial matrix] ... TRNT1 polymerizes CCA at the 3 end of pre-tRNA (Homo sapiens) ...
It is not necessary to enrich for small RNA. If, however, it is desired to work with small RNA samples, small RNA represents ... To go from sample to sequencer, the process takes only eight hours, with minimal hands-on time. Up to a maximum of 48 samples ... Samples and data generated with single-end indexed libraries should only be compared with other samples and data generated with ... "RNA fragments or other small RNAs that arent miRNAs"; these are common to see in total RNA samples, being particularly strong ...
... end of viral RNA (U3), and from sequences repeated at both ends of the viral RNA. The integrated provirus is larger than the ... Reverse transcriptase is the essential enzyme that carries out the reverse transcription process that take the RNA genome to a ... end of viral RNA (U5), from sequences unique to the 3′ ... The RNA genome in the free retrovirus is arranged as a diploid ... They use an integrase that acts in a site-specific manner to join the ends of the viral cDNA to target sequences in host cell ...